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Showing :
featureCounts
•
reset
0
votes
0
replies
219
views
Blank gene_id in featureCounts
featureCounts
Rsubread
4 months ago
Meg
• 0
5
votes
10
replies
2.4k
views
Feature Counts - potential bug with outfile.jcounts
featurecounts
Rsubread
written 9 months ago by
chris2.a.white
• 0
0
votes
1
reply
890
views
To count or not to count multi-overlapping reads?
RNA-Seq
featureCounts
Rsubread
updated 7 months ago by
Gordon Smyth
53k • written 7 months ago by
Arindam
▴ 80
0
votes
1
reply
803
views
Feature Counts -R XS Read Assignment Tag
FeatureCounts
Rsubread
updated 9 months ago by
Gordon Smyth
53k • written 19 months ago by
chris2.a.white
• 0
1
vote
3
replies
1.6k
views
featureCounts fails to load annotation file
featurecounts
rnas
RNASeqPower
11 months ago
yasmin.hilliam
▴ 10
0
votes
1
reply
579
views
[BUG] featureCounts doesn't assign primary alignment of a multi-mapped read, even with `--primary` specified
featureCounts
Rsubread
12 months ago
isaac.vock
• 0
6
votes
5
replies
4.0k
views
What is the importance of `--countReadPairs` in featureCounts on paired end data?
featureCounts
DESeq2
updated 9 months ago by
rohitsatyam102
▴ 20 • written 13 months ago by
Bioinformagician
• 0
0
votes
4
replies
1.1k
views
featureCounts error: Assertion `numOfBuckets > 0' failed
featureCounts
Subread
Bioconductor
14 months ago
Jessica
• 0
1
vote
2
replies
858
views
mates counted separately in case one mate is unmapped (when otherwise counting read pairs)
featureCounts
Rsubread
14 months ago
Anke
• 0
4
votes
2
replies
1.8k
views
Behaviour of featureCounts when quantifying multi-mapping reads
Bioconductor
featurecounts
subread
15 months ago
Rachael
• 0
2
votes
4
replies
1.5k
views
Odd behavior of Rsubread::featureCounts when setting `nonOverlap = 0`
featureCounts
Rsubread
18 months ago • updated 15 months ago
isaac.vock
• 0
1
vote
1
reply
1.0k
views
featureCounts unable to assign long reads to one specific isoform
featureCounts
Rsubread
updated 19 months ago by
Wei Shi
★ 3.6k • written 20 months ago by
Alex
• 0
7
votes
28
replies
8.3k
views
Rsubread::featureCounts extremely slow on some annotations
rsubread
featurecounts
updated 20 months ago by
Gordon Smyth
53k • written 10.6 years ago by
Ryan C. Thompson
★ 7.9k
0
votes
4
replies
1.4k
views
Adjusting featureCounts settings in Galaxy for miRNA overlaps
featureCounts
Rsubread
updated 20 months ago by
Gordon Smyth
53k • written 20 months ago by
tjgray4
• 0
4
votes
6
replies
1.6k
views
[BUG] Rsubread::featureCounts quirk in CORE files: different target list ordering for same target lists
featurecounts
Rsubread
20 months ago
isaac.vock
• 0
0
votes
2
replies
4.8k
views
Dealing with multi-overlapping and multi-mapping reads
RNA-seq
featureCounts
ht-seq
written 5.2 years ago by
Arindam
▴ 80
0
votes
0
replies
2.0k
views
Tutorial:
Read data file containing both counts and annotation into edgeR
rpkm
edgeR
featureCounts
2.1 years ago
Gordon Smyth
53k
0
votes
2
replies
2.4k
views
understanding differences in RSEM, STAR outputs and featureCounts
RNASeqData
Rsubread
mapping
featurecounts
updated 2.2 years ago by
James W. MacDonald
68k • written 2.2 years ago by
vitoriastavis
• 0
1
vote
1
reply
1.2k
views
featureCounts bug with unpaired reads
Rsubread
featureCounts
updated 2.2 years ago by
Yang Liao
▴ 450 • written 2.2 years ago by
StevenE.Strong
• 0
0
votes
1
reply
1.5k
views
Potential segfault bug in featureCounts using long read data
Bioconductor
Rsubread
featureCounts
updated 2.4 years ago by
Wei Shi
★ 3.6k • written 2.4 years ago by
Carlos
• 0
0
votes
0
replies
773
views
How to extract the counts for non-split reads from paired end RNA-seq data?
rsubread
featurecounts
Bioconductor
countsextraction
2.5 years ago
barrypraveen
• 0
2
votes
9
replies
3.2k
views
featureCounts with NCBI T2T not capturing all genes
CHM13v2.0
featureCounts
T2T
Rsubread
2.6 years ago
Hilary
▴ 40
0
votes
2
replies
1.4k
views
Possible bugs in Rsubread/stad-alone featureCounts options fracOverlap and largestOverlap with fractional counts
Rsubread
featureCounts
updated 2.6 years ago by
Wei Shi
★ 3.6k • written 2.8 years ago by
Konstantin
• 0
0
votes
0
replies
1.1k
views
Subread2.0.3 featureCounts segmentation fault when loading gtf file
Subread
GTF
featureCounts
segmentationfault
2.6 years ago
yao hua
• 0
0
votes
1
reply
1.4k
views
Subread installation on Linux ARM64
subread
featureCounts
updated 2.7 years ago by
Yang Liao
▴ 450 • written 2.7 years ago by
Keyu
• 0
0
votes
1
reply
1.1k
views
DEG analysis with Paired-end RNA-seq data using DESeq2, do I need to contain the library type when I using DESeq() function if I already using featur…
DESeq2
featurecounts
updated 2.7 years ago by
swbarnes2
★ 1.4k • written 2.7 years ago by
Bo
• 0
3
votes
3
replies
6.7k
views
Why is it better to count genes at the exon-level using subread::featureCounts()?
RNASeq
Rsubread
featureCounts
Quantification
updated 2.7 years ago by
Gordon Smyth
53k • written 2.7 years ago by
Sara
• 0
0
votes
1
reply
3.8k
views
FeatureCounts: Low assigned reads, can I proceed? Do I need to do another pre-processing step?
LowAssignedReads
STAR
featureCounts
updated 2.8 years ago by
swbarnes2
★ 1.4k • written 2.8 years ago by
lalala
• 0
1
vote
1
reply
3.7k
views
Quantification of Genes with RSubread::featureCounts() at exon-level vs gene-level?
Subread
RNASeq
featureCounts
quantification
updated 2.9 years ago by
Gordon Smyth
53k • written 2.9 years ago by
Sara
• 0
0
votes
0
replies
956
views
Option request for featureCounts: Add an order of read manipulation to 'shift > reduction > extension'
SubRead
featureCounts
updated 2.9 years ago by
ATpoint
★ 4.9k • written 2.9 years ago by
Leon
• 0
2
votes
3
replies
2.3k
views
Rsubread featureCounts outputs dozens of temp files, no counts
RNAseq
R
Rsubread
featurecounts
RNASeq
updated 2.9 years ago by
Gordon Smyth
53k • written 2.9 years ago by
Jon
• 0
2
votes
2
replies
1.3k
views
featureCounts: No effect of setting -d and -D?
subread
featureCounts
3.0 years ago
dario.beraldi
• 0
0
votes
0
replies
907
views
Correct GFF3 translation for FeatureCounts
RNASeq
FeatureCounts
Rna
3.0 years ago
rridley3
• 0
0
votes
2
replies
2.5k
views
ERROR: failed to find the gene identifier attribute in the 9th column of the provided GTF file.
FeatureCounts
3.1 years ago
KMS
▴ 20
0
votes
1
reply
5.2k
views
gff3 file for featureCounts
gtf/gff3
subread
featureCounts
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
selmanurkeskin
• 0
0
votes
1
reply
1.2k
views
fetaure counts err
featurecounts
updated 3.2 years ago by
Wei Shi
★ 3.6k • written 3.2 years ago by
哲
• 0
0
votes
1
reply
1.3k
views
DEXSeq Exon count not working, please help with an alternative like featureCounts
splicing
Exon
featureCounts
subread
DEXSeq
3.4 years ago • updated 3.3 years ago
NIK
• 0
1
vote
1
reply
2.1k
views
Using featureCounts and downloading Rsubread
featureCounts
RNASeq
Rsubread
R
updated 3.4 years ago by
Gordon Smyth
53k • written 3.4 years ago by
margo
• 0
2
votes
1
reply
2.2k
views
Difference between raw counts and featureCounts for DESeq2
deseq
featurecounts
counts
DESeq2
updated 3.4 years ago by
swbarnes2
★ 1.4k • written 3.4 years ago by
Hicham
▴ 10
0
votes
4
replies
2.6k
views
featureCounts: 0% successfully assigned fragments
featureCounts
3.4 years ago
KMS
▴ 20
1
vote
4
replies
2.4k
views
Annotated file with gene ID (instead of gene symbol)
Rsubread
featureCounts
3.4 years ago
Chise
▴ 10
3
votes
11
replies
5.6k
views
The low successful assignment ratio of FeatureCounts
FeatureCounts
Rsubread
Subread
3.5 years ago • updated 3.4 years ago
Sarah_piggy
▴ 10
1
vote
4
replies
2.8k
views
Subread installation
subread
featureCounts
3.5 years ago
Lucy
▴ 60
1
vote
5
replies
3.3k
views
featureCounts parameter queries - minOverlap, fracOverlap, fracOverlapFeature
subread
Rsubread
featureCounts
RNA-seq
3.6 years ago
Lucy
▴ 60
2
votes
2
replies
8.4k
views
"Paired-end reads were detected in single-end read library"
featureCounts
Rsubread
3.8 years ago
Chise
▴ 10
4
votes
4
replies
6.6k
views
FeatureCounts
counts
subread
RNASeq
featurecounts
3.9 years ago
k___
▴ 30
0
votes
2
replies
2.1k
views
featureCounts: unable to find chromosome in SAM header
RNASeq
featureCounts
updated 3.0 years ago by
marie
• 0 • written 4.1 years ago by
nklier38
• 0
1
vote
1
reply
1.8k
views
Make featureCounts ignore soft clip and insertions when for calculating read overlap
subread
featureCounts
updated 4.2 years ago by
Wei Shi
★ 3.6k • written 4.2 years ago by
dario.beraldi
• 0
1
vote
5
replies
7.2k
views
featureCounts (subread v2.0.2) ERROR: invalid parameter: '−−countReadPairs'
subread
featureCounts
updated 2.2 years ago by
Yang Liao
▴ 450 • written 4.3 years ago by
s.muroy
▴ 10
4
votes
5
replies
3.0k
views
Rsubread issue with featurecounts :----------- FAILURE REPORT -------------- --- failure: length > 1 in coercion to logical --- --- srcref ---
Rsubread
featurecounts
coercion
4.3 years ago • updated 4.2 years ago
Konstantinos Yeles
▴ 90
136 results • Page
1 of 3
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Replies
Comment: incomplete imputation of missing values with LEA impute() function
by
Gordon Smyth
53k
Nadia, please tag your question with the name of the package ("LEA") that you are asking about. "impute" is the name of a different Biocond…
Comment: Sharing a collection of datasets in ExperimentHub
by
Aaron Lun
★ 29k
FYI the **scRNAseq** packages uses a non-ExperimentHub storage platform, see [here](https://github.com/ArtifactDB/gypsum-worker) for more d…
Comment: Error Loading Experiment from SimBenchData Package
by
ATpoint
★ 4.9k
Is the Seurat package installed? The error somewhat suggests that it could be related, and at https://www.bioconductor.org/packages/release…
Answer: Help with defining groups
by
Gordon Smyth
53k
Defining sample groups is separate to reading in the DIA-NN data, because the sample conditions and covariates are not necessarily coded in…
Comment: Sharing a collection of datasets in ExperimentHub
by
Peter Hickey
▴ 760
The [**scRNAseq**](https://bioconductor.org/packages/scRNAseq/) package ([source on GitHub](https://github.com/LTLA/scRNAseq)) might serve …
Votes
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
Answer: ordinary t from limma [was: How would I normally compare swirl?]
Comment: readDNAStringSet with non DNA characters
Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
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