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Showing :
featureCounts
•
reset
0
votes
2
replies
176
views
featureCounts error: Assertion `numOfBuckets > 0' failed
featureCounts
Subread
Bioconductor
9 days ago
Jessica
• 0
4
votes
2
replies
284
views
Behaviour of featureCounts when quantifying multi-mapping reads
Bioconductor
featurecounts
subread
6 weeks ago
Rachael
• 0
2
votes
4
replies
696
views
Odd behavior of Rsubread::featureCounts when setting `nonOverlap = 0`
featureCounts
Rsubread
3 months ago • updated 6 weeks ago
isaac.vock
• 0
0
votes
0
replies
236
views
Feature Counts -R XS Read Assignment Tag
FeatureCounts
Rsubread
4 months ago
chris2.a.white
• 0
1
vote
1
reply
416
views
featureCounts unable to assign long reads to one specific isoform
featureCounts
Rsubread
updated 4 months ago by
Wei Shi
★ 3.6k • written 5 months ago by
Alex
• 0
7
votes
28
replies
6.5k
views
Rsubread::featureCounts extremely slow on some annotations
rsubread
featurecounts
updated 5 months ago by
Gordon Smyth
51k • written 9.4 years ago by
Ryan C. Thompson
★ 7.9k
0
votes
4
replies
601
views
Adjusting featureCounts settings in Galaxy for miRNA overlaps
featureCounts
Rsubread
updated 5 months ago by
Gordon Smyth
51k • written 5 months ago by
tjgray4
• 0
4
votes
6
replies
885
views
[BUG] Rsubread::featureCounts quirk in CORE files: different target list ordering for same target lists
featurecounts
Rsubread
6 months ago • updated 5 months ago
isaac.vock
• 0
0
votes
2
replies
3.8k
views
Dealing with multi-overlapping and multi-mapping reads
RNA-seq
featureCounts
ht-seq
written 4.0 years ago by
ag1805x
▴ 80
0
votes
0
replies
1.2k
views
Tutorial:
Read data file containing both counts and annotation into edgeR
rpkm
edgeR
featureCounts
11 months ago • updated 10 months ago
Gordon Smyth
51k
0
votes
2
replies
1.3k
views
understanding differences in RSEM, STAR outputs and featureCounts
RNASeqData
Rsubread
mapping
featurecounts
updated 11 months ago by
James W. MacDonald
66k • written 11 months ago by
vitoriastavis
• 0
1
vote
1
reply
757
views
featureCounts bug with unpaired reads
Rsubread
featureCounts
updated 12 months ago by
Yang Liao
▴ 440 • written 12 months ago by
StevenE.Strong
• 0
0
votes
1
reply
997
views
Potential segfault bug in featureCounts using long read data
Bioconductor
Rsubread
featureCounts
updated 14 months ago by
Wei Shi
★ 3.6k • written 15 months ago by
Carlos
• 0
0
votes
0
replies
479
views
How to extract the counts for non-split reads from paired end RNA-seq data?
rsubread
featurecounts
Bioconductor
countsextraction
15 months ago
barrypraveen
• 0
2
votes
9
replies
1.9k
views
featureCounts with NCBI T2T not capturing all genes
CHM13v2.0
featureCounts
T2T
Rsubread
16 months ago
Hilary
▴ 40
0
votes
2
replies
971
views
Possible bugs in Rsubread/stad-alone featureCounts options fracOverlap and largestOverlap with fractional counts
Rsubread
featureCounts
updated 17 months ago by
Wei Shi
★ 3.6k • written 20 months ago by
Konstantin
• 0
0
votes
0
replies
716
views
Subread2.0.3 featureCounts segmentation fault when loading gtf file
Subread
GTF
featureCounts
segmentationfault
17 months ago
yao hua
• 0
0
votes
1
reply
901
views
Subread installation on Linux ARM64
subread
featureCounts
updated 17 months ago by
Yang Liao
▴ 440 • written 18 months ago by
Keyu
• 0
0
votes
1
reply
660
views
DEG analysis with Paired-end RNA-seq data using DESeq2, do I need to contain the library type when I using DESeq() function if I already using featur…
DESeq2
featurecounts
updated 18 months ago by
swbarnes2
★ 1.4k • written 18 months ago by
Bo
• 0
3
votes
3
replies
3.3k
views
Why is it better to count genes at the exon-level using subread::featureCounts()?
RNASeq
Rsubread
featureCounts
Quantification
updated 18 months ago by
Gordon Smyth
51k • written 18 months ago by
Sara
• 0
0
votes
1
reply
2.3k
views
FeatureCounts: Low assigned reads, can I proceed? Do I need to do another pre-processing step?
LowAssignedReads
STAR
featureCounts
updated 20 months ago by
swbarnes2
★ 1.4k • written 20 months ago by
lalala
• 0
1
vote
1
reply
2.2k
views
Quantification of Genes with RSubread::featureCounts() at exon-level vs gene-level?
Subread
RNASeq
featureCounts
quantification
updated 20 months ago by
Gordon Smyth
51k • written 20 months ago by
Sara
• 0
0
votes
0
replies
633
views
Option request for featureCounts: Add an order of read manipulation to 'shift > reduction > extension'
SubRead
featureCounts
updated 20 months ago by
ATpoint
★ 4.2k • written 20 months ago by
Leon
• 0
2
votes
3
replies
1.4k
views
Rsubread featureCounts outputs dozens of temp files, no counts
RNAseq
R
Rsubread
featurecounts
RNASeq
updated 20 months ago by
Gordon Smyth
51k • written 20 months ago by
Jon
• 0
2
votes
2
replies
862
views
featureCounts: No effect of setting -d and -D?
subread
featureCounts
21 months ago
dario.beraldi
• 0
0
votes
0
replies
587
views
Correct GFF3 translation for FeatureCounts
RNASeq
FeatureCounts
Rna
21 months ago
rridley3
• 0
0
votes
2
replies
1.6k
views
ERROR: failed to find the gene identifier attribute in the 9th column of the provided GTF file.
FeatureCounts
23 months ago • updated 22 months ago
KMS
▴ 20
0
votes
1
reply
3.7k
views
gff3 file for featureCounts
gtf/gff3
subread
featureCounts
updated 2.0 years ago by
Gordon Smyth
51k • written 2.0 years ago by
selmanurkeskin
• 0
0
votes
1
reply
848
views
fetaure counts err
featurecounts
updated 2.0 years ago by
Wei Shi
★ 3.6k • written 2.1 years ago by
哲
• 0
0
votes
1
reply
952
views
DEXSeq Exon count not working, please help with an alternative like featureCounts
splicing
Exon
featureCounts
subread
DEXSeq
2.2 years ago
NIK
• 0
1
vote
1
reply
1.5k
views
Using featureCounts and downloading Rsubread
featureCounts
RNASeq
Rsubread
R
updated 2.2 years ago by
Gordon Smyth
51k • written 2.2 years ago by
margo
• 0
2
votes
1
reply
1.6k
views
Difference between raw counts and featureCounts for DESeq2
deseq
featurecounts
counts
DESeq2
updated 2.2 years ago by
swbarnes2
★ 1.4k • written 2.2 years ago by
Hicham
▴ 10
0
votes
4
replies
1.9k
views
featureCounts: 0% successfully assigned fragments
featureCounts
2.2 years ago
KMS
▴ 20
1
vote
4
replies
1.6k
views
Annotated file with gene ID (instead of gene symbol)
Rsubread
featureCounts
2.2 years ago
Chise
▴ 10
3
votes
11
replies
4.0k
views
The low successful assignment ratio of FeatureCounts
FeatureCounts
Rsubread
Subread
2.3 years ago • updated 2.2 years ago
Sarah_piggy
▴ 10
1
vote
4
replies
1.8k
views
Subread installation
subread
featureCounts
2.3 years ago
Lucy
▴ 60
1
vote
5
replies
2.2k
views
featureCounts parameter queries - minOverlap, fracOverlap, fracOverlapFeature
subread
Rsubread
featureCounts
RNA-seq
2.5 years ago • updated 2.4 years ago
Lucy
▴ 60
2
votes
2
replies
6.5k
views
"Paired-end reads were detected in single-end read library"
featureCounts
Rsubread
2.6 years ago
Chise
▴ 10
4
votes
4
replies
5.3k
views
FeatureCounts
counts
subread
RNASeq
featurecounts
2.7 years ago
k___
▴ 30
0
votes
2
replies
1.6k
views
featureCounts: unable to find chromosome in SAM header
RNASeq
featureCounts
updated 21 months ago by
marie
• 0 • written 2.9 years ago by
nklier38
• 0
1
vote
1
reply
1.3k
views
Make featureCounts ignore soft clip and insertions when for calculating read overlap
subread
featureCounts
updated 3.0 years ago by
Wei Shi
★ 3.6k • written 3.0 years ago by
dario.beraldi
• 0
1
vote
5
replies
5.1k
views
featureCounts (subread v2.0.2) ERROR: invalid parameter: '−−countReadPairs'
subread
featureCounts
updated 12 months ago by
Yang Liao
▴ 440 • written 3.1 years ago by
s.muroy
▴ 10
4
votes
5
replies
2.3k
views
Rsubread issue with featurecounts :----------- FAILURE REPORT -------------- --- failure: length > 1 in coercion to logical --- --- srcref ---
Rsubread
featurecounts
coercion
3.1 years ago • updated 3.0 years ago
Konstantinos Yeles
▴ 80
2
votes
2
replies
2.0k
views
Error when loading annotation featureCounts
featureCounts
Rsubread
subread
CellBiology
updated 3.4 years ago by
Yang Liao
▴ 440 • written 3.4 years ago by
Jason
• 0
0
votes
0
replies
831
views
Are SEQ-QVALs SAM/BAM fields necessary in the workflow of featureCounts?
Rsubread
featureCounts
3.7 years ago
martinag.work
• 0
1
vote
2
replies
3.8k
views
ERROR: failed to find the gene identifier attribute in the 9th column of the provided GTF file.
featureCounts
4.3 years ago
chatterjee.arumoy
▴ 10
2
votes
3
replies
2.0k
views
RNAseq read counts generated by FeatureCounts
featurecounts
RNAseq
updated 4.7 years ago by
James W. MacDonald
66k • written 4.7 years ago by
adeler001
• 0
4
votes
2
replies
1.3k
views
bwa mem to feature Counts
bwa mem
featureCounts
updated 4.8 years ago by
swbarnes2
★ 1.4k • written 4.8 years ago by
ajajoo
• 0
0
votes
2
replies
1.1k
views
Problem in featureCounts file.
rna-seq
featureCounts
SubRead
updated 5.0 years ago by
Wei Shi
★ 3.6k • written 5.0 years ago by
hafiz.talhamalik
• 0
4
votes
3
replies
4.5k
views
Rsubread, Error in featureCounts paired end
Rsubread
paired end
featureCounts
updated 5.0 years ago by
Wei Shi
★ 3.6k • written 5.1 years ago by
hauken_heyken
▴ 80
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Comment: 'path' must be string
by
James W. MacDonald
66k
Yes. We already knew that from the error message, and I told you that in my previous email. The point wasn't to figure out *where* the err…
Comment: 'path' must be string
by
cpierce2
• 0
I ran the debugger and this is the line that causes the error: ``` if (any(startsWith(pkcData[["RTS_ID"]][1L], "RTS")) & any(startsWith(pr…
Comment: Generating surrogate variables across multiple variables of interest
by
Robert
• 0
Yes, we have several biomarkers, and a proteomic set, so we are characterizing each biomarker by it's proteomic association(s). The use for…
Comment: Error running tximeta
by
Guido Hooiveld
★ 4.0k
FYI (since I just finalized a run with `salmon`). For each sample (`.`) an output folder is created that contains 3 files (`cmd_info.json`…
Comment: Error running tximeta
by
Michael Love
42k
Tximeta uses the full directory. There are files that give the key information about the transcriptome. It's not a good idea to selectively…
Votes
Question: Deconvolution Methods on RNA-Seq Data (Mixed cell types)
Answer: Understanding how to contrast two treatments in DESeq2
Answer: Understanding how to contrast two treatments in DESeq2
Cannot extract TxFeatures for a defined GRanges object
Limma time series spline help
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