Showing : genomicalignments • reset
4
votes
5
replies
774
views
Strand information in a GAlignmentsList object derived from strand-specific paired-end RNA-seq data
GenomicAlignments
14 months ago Robert Castelo ★ 3.2k
1
vote
0
replies
537
views
Possible to customize the parallel computation used in readGAlignments?
R readGAlignments GenomicAlignments
18 months ago chen_ying ▴ 10
3
votes
4
replies
966
views
mapToAlignments_issue
GenomicAlignments
updated 20 months ago by 16k • written 20 months ago by • 0
0
votes
1
reply
1.2k
views
Determining alignment coordinates from BAM file
GenomicAlignments GenomicA
updated 22 months ago by • 0 • written 22 months ago by • 0
0
votes
9
replies
1.5k
views
Subsetting operation on CompressedGRangesList object 'x' produces a result that is too big to be represented as a CompressedList object.
DegNorm GenomicAlignments
2.0 years ago • updated 23 months ago Jiping Wang ▴ 90
1
vote
0
replies
949
views
How could I include the unmapped reads of a BAM file for further analysis?
GenomicAlignments Biostrings Rsamtools
2.1 years ago L ▴ 50
0
votes
1
reply
3.0k
views
Error: BiocParallel errors
Bioconductor metagene GenomicAlignments
updated 21 months ago by • 0 • written 2.2 years ago by • 0
0
votes
0
replies
541
views
readGAlignmentPairs() hanging while reading reads in specified intervals
genomicalig GenomicAlignments
3.0 years ago Malcolm Perry ▴ 40
0
votes
1
reply
1.8k
views
Error in GenomicAlignments::summarizeOverlaps: attempt to select less than one element in OneIndex
SummarizedExperiment GenomicAlignments
3.1 years ago Matthias Munz ▴ 10
0
votes
2
replies
899
views
Visualising read alignments in
GenomicAlignments Rsamtools
written 3.1 years ago by • 0
4
votes
4
replies
3.3k
views
Best way to handle tags of bam files
GenomicAlignments bam Rsamtools
written 3.1 years ago by ▴ 20
0
votes
3
replies
936
views
first(aln) on GAlignmentPairs object always and only return 2 entries
readGAlignmentPairs GenomicAlignments
3.4 years ago Marco Blanchette ▴ 30
0
votes
0
replies
799
views
Counting reads in genes with summarizeOverlaps
genomicAlignments summarizeOverlaps
3.8 years ago dequattro.concetta ▴ 10
0
votes
0
replies
1.5k
views
summarizeOverlaps - (GenomicAlignments) - gives warnings 'package:stats' may not be available when loading
GenomicAlignments summerizeOverlaps-Method DEXSeq
updated 4.0 years ago by 25k • written 4.0 years ago by ▴ 10
1
vote
2
replies
829
views
Rsamtools/GenomicAlignments segmentation faults
Rsamtools GenomicAlignments
updated 4.0 years ago by 25k • written 4.0 years ago by ▴ 10
4
votes
4
replies
1.6k
views
Issues loading a BAM file resulted from featurecounts
featurecount rsubread GenomicAlignments
4.1 years ago Konstantinos Yeles ▴ 80
2
votes
2
replies
1.4k
views
"S" bases on mapFromAlignments - counted or not?
genomicalignments
updated 4.2 years ago by 16k • written 4.2 years ago by • 0
0
votes
8
replies
1.6k
views
exonsBy does not order exons by ascending rank, is it a bug or outdated documentation?
GenomicFeatures GenomicAlignment GenomicAlignments exonBy coverageByTranscript
updated 4.4 years ago by 16k • written 4.4 years ago by ▴ 90
0
votes
2
replies
783
views
Should GenomicAlignments::mapToAlignments() terminate when no alignments are found for one read?
GenomicAlignments
updated 4.4 years ago by ★ 6.8k • written 4.5 years ago by ▴ 40
4
votes
6
replies
1.6k
views
How to query a base along an aligned read by genomic position?
GenomicAlignments BAM CIGAR
updated 4.5 years ago by ▴ 740 • written 4.5 years ago by ▴ 40
1
vote
2
replies
1.5k
views
Coverage function assigns wrong weights for gapped alignments
genomicalignments splicing coverage
updated 4.5 years ago by ★ 11k • written 7.0 years ago by ▴ 10
0
votes
4
replies
892
views
coverageByTranscript coudble counts overlapped region for paired-end data
GenomicAlignments RNA-seq paired-end read coverage
updated 4.7 years ago by 16k • written 4.7 years ago by ▴ 90
1
vote
9
replies
1.4k
views
stackStringsFromBam equivalent for GAlignments with seq
genomicalignments stackStringsFromBam sequenceLayer
4.8 years ago Janet Young ▴ 740
1
vote
8
replies
2.6k
views
How to use Rsamtool or GenomicAlignments to calculate RAN-seq read coverage curves
Rsamtools GenomicAlignments RNA-seq coverage score
updated 4.9 years ago by 16k • written 4.9 years ago by ▴ 90
4
votes
4
replies
1.1k
views
Mimic Counting of HTSeq-count Using countOverlaps
GenomicAlignments countOverlaps
updated 4.9 years ago by 25k • written 4.9 years ago by ★ 1.5k
0
votes
9
replies
1.4k
views
summarizeOverlaps bplapply error in DeSeq2 pipeline
summarizeOverlaps R GenomicAlignments
updated 4.9 years ago by 25k • written 4.9 years ago by • 0
3
votes
3
replies
993
views
How to tell a GAlignmentsList singleton is mate1 or mate2
Rsamtools GenomicAlignments GAlignmentsList
updated 5.0 years ago by 65k • written 5.0 years ago by ▴ 100
3
votes
2
replies
1.2k
views
Error in summarizeOverlaps
GenomicAlignments
updated 5.0 years ago by 65k • written 5.0 years ago by • 0
2
votes
1
reply
1.0k
views
Iterating over BAM file by genomic chunk
GenomicAlignments
updated 5.1 years ago by 25k • written 5.1 years ago by 65k
0
votes
2
replies
888
views
Duplicated reads if 'which' has overlapping reads in ScanBamParam
documentation Rsamtools GenomicAlignments
5.1 years ago Christian Mertes • 0
2
votes
3
replies
1.2k
views
indexBam(), summarizeOverlaps() error: RNA-seq
genomicAlignments RNA-seq Rsamtools
updated 5.2 years ago by ★ 6.5k • written 5.2 years ago by ▴ 70
1
vote
3
replies
1.1k
views
Efficient counting of nucleotide changes in short-reads
GenomicAlignments Biostrings
updated 5.4 years ago by ★ 11k • written 5.4 years ago by ★ 3.2k
0
votes
1
reply
1.2k
views
Subset bam file in R
rsamtools genomicalignments genomicranges
updated 5.4 years ago by ★ 11k • written 5.4 years ago by • 0
0
votes
2
replies
959
views
Preparation of summarizedExperiment for dexSeq
dexseq genomicalignments genomicfeatures genomicranges
5.5 years ago A ▴ 40
0
votes
2
replies
1.3k
views
Extracting fasta sequences from a RE digested genome .bed file
biostrings genomicalignments rsamtools
updated 5.6 years ago by 16k • written 5.7 years ago by • 0
1
vote
1
reply
1.3k
views
How to parse supplementary (not secondary) alignments
genomicalignments rsamtools
updated 5.7 years ago by 25k • written 5.7 years ago by ▴ 30
0
votes
1
reply
798
views
Scoring DNA motifs by conservation
conservation sequence genomicalignments
5.7 years ago martin.busch • 0
2
votes
2
replies
1.1k
views
How to write a preprocess.reads function for summarizeOverlaps that requires different logic for paired and single end reads?
summarizeoverlaps genomicalignments
updated 6.0 years ago by ★ 6.8k • written 6.0 years ago by ★ 7.9k
0
votes
0
replies
1.1k
views
large memory footprint with summarizeOverlaps method for BamViews
genomicalignments rsamtools BamViews summarizeoverlaps
updated 6.1 years ago by 25k • written 6.1 years ago by ▴ 10
1
vote
4
replies
1.5k
views
error displaying bam files
genomicalignments
6.4 years ago kamal.fartiyal84 ▴ 10
5
votes
8
replies
2.2k
views
How to set 'strandMode' with summarizeOverlaps()
GenomicAlignments
6.4 years ago Robert Castelo ★ 3.2k
2
votes
4
replies
1.5k
views
How to build a RNA-seq table of counts for repeatmasker annotations
GenomicFeatures repeatmasker GenomicAlignments
updated 6.5 years ago by ★ 11k • written 6.5 years ago by ★ 3.2k
2
votes
9
replies
2.1k
views
FDR adjustment in Time-Course DATA DESEQ2
deseq2 fdrtool genomicalignments
updated 6.5 years ago by 41k • written 6.5 years ago by ▴ 20
0
votes
0
replies
999
views
Counting exonic reads with DEXSeq and GenomicAlignments
dexseq genomicalignments exon usage
6.5 years ago igor ▴ 40
5
votes
4
replies
2.4k
views
how to extract read alignments from reads aligning in mulitple locations
rsamtools genomicalignments genomicfeatures
updated 6.8 years ago by ★ 1.5k • written 6.8 years ago by ★ 3.2k
2
votes
5
replies
1.7k
views
NGS with summarizeOverlaps and SAM files
genomicalignments
updated 6.9 years ago by 65k • written 6.9 years ago by • 0
2
votes
1
reply
1.2k
views
GenomicAlignments/access correct bp positions near indels
genomicranges genomicalignments snp
updated 7.0 years ago by 16k • written 7.0 years ago by ▴ 90
3
votes
2
replies
1.4k
views
How are duplicates determined with readGAlignments/scanBam?
genomicalignments rsamtools
updated 7.1 years ago by ★ 6.5k • written 7.1 years ago by ▴ 30
0
votes
1
reply
1.1k
views
GenomicAlignment: Import genomic intervals described in a bed file into "summarizeOverlaps"
GenomicAlignments summarizeoverlaps
updated 7.2 years ago by 65k • written 7.2 years ago by ▴ 40
2
votes
2
replies
1.2k
views
Integer overflow when tabulating read coverage
genomicalignments genomicranges
updated 7.2 years ago by ★ 11k • written 7.2 years ago by ▴ 70
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I am not entirely clear what you mean by "technical" replicates in this case. Are they resequencing of the same RNA samples or are they fre…
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Hi, I'm hitting this too. As far as I can tell my DataTrack is fine, but perhaps I am missing something. Notably the same code works on o…
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Hi Robert, If I don't want to use all pathway in c2 gene set but only a few pathways of interested, how can I do that? So we try to get a …
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Hi Michael, thanks for the answer! I'll have a look : )
Comment: DESeq filtering specific to contrasts by Michael Love 41k
It will likely not filter it out, but really the per-dataset details would affect the results.
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