Showing : genomicalignments • reset
4
votes
5
replies
793
views
Strand information in a GAlignmentsList object derived from strand-specific paired-end RNA-seq data
GenomicAlignments
15 months ago • updated 14 months ago Robert Castelo ★ 3.3k
1
vote
0
replies
548
views
Possible to customize the parallel computation used in readGAlignments?
R readGAlignments GenomicAlignments
18 months ago chen_ying ▴ 10
3
votes
4
replies
978
views
mapToAlignments_issue
GenomicAlignments
updated 20 months ago by 16k • written 21 months ago by • 0
0
votes
1
reply
1.2k
views
Determining alignment coordinates from BAM file
GenomicAlignments GenomicA
updated 23 months ago by • 0 • written 23 months ago by • 0
0
votes
9
replies
1.6k
views
Subsetting operation on CompressedGRangesList object 'x' produces a result that is too big to be represented as a CompressedList object.
DegNorm GenomicAlignments
2.0 years ago • updated 23 months ago Jiping Wang ▴ 90
1
vote
0
replies
966
views
How could I include the unmapped reads of a BAM file for further analysis?
GenomicAlignments Biostrings Rsamtools
2.1 years ago L ▴ 50
0
votes
1
reply
3.1k
views
Error: BiocParallel errors
Bioconductor metagene GenomicAlignments
updated 22 months ago by • 0 • written 2.3 years ago by • 0
0
votes
0
replies
552
views
readGAlignmentPairs() hanging while reading reads in specified intervals
genomicalig GenomicAlignments
3.0 years ago Malcolm Perry ▴ 40
0
votes
1
reply
1.8k
views
Error in GenomicAlignments::summarizeOverlaps: attempt to select less than one element in OneIndex
SummarizedExperiment GenomicAlignments
3.1 years ago Matthias Munz ▴ 10
0
votes
2
replies
911
views
Visualising read alignments in
GenomicAlignments Rsamtools
written 3.1 years ago by • 0
4
votes
4
replies
3.4k
views
Best way to handle tags of bam files
GenomicAlignments bam Rsamtools
written 3.2 years ago by ▴ 20
0
votes
3
replies
944
views
first(aln) on GAlignmentPairs object always and only return 2 entries
readGAlignmentPairs GenomicAlignments
3.4 years ago Marco Blanchette ▴ 30
0
votes
0
replies
809
views
Counting reads in genes with summarizeOverlaps
genomicAlignments summarizeOverlaps
3.8 years ago dequattro.concetta ▴ 10
0
votes
0
replies
1.5k
views
summarizeOverlaps - (GenomicAlignments) - gives warnings 'package:stats' may not be available when loading
GenomicAlignments summerizeOverlaps-Method DEXSeq
updated 4.0 years ago by 25k • written 4.0 years ago by ▴ 10
1
vote
2
replies
838
views
Rsamtools/GenomicAlignments segmentation faults
Rsamtools GenomicAlignments
updated 4.1 years ago by 25k • written 4.1 years ago by ▴ 10
4
votes
4
replies
1.6k
views
Issues loading a BAM file resulted from featurecounts
featurecount rsubread GenomicAlignments
4.1 years ago Konstantinos Yeles ▴ 80
2
votes
2
replies
1.4k
views
"S" bases on mapFromAlignments - counted or not?
genomicalignments
updated 4.2 years ago by 16k • written 4.2 years ago by • 0
0
votes
8
replies
1.6k
views
exonsBy does not order exons by ascending rank, is it a bug or outdated documentation?
GenomicFeatures GenomicAlignment GenomicAlignments exonBy coverageByTranscript
updated 4.5 years ago by 16k • written 4.5 years ago by ▴ 90
0
votes
2
replies
790
views
Should GenomicAlignments::mapToAlignments() terminate when no alignments are found for one read?
GenomicAlignments
updated 4.5 years ago by ★ 6.8k • written 4.5 years ago by ▴ 40
4
votes
6
replies
1.6k
views
How to query a base along an aligned read by genomic position?
GenomicAlignments BAM CIGAR
updated 4.5 years ago by ▴ 740 • written 4.5 years ago by ▴ 40
1
vote
2
replies
1.5k
views
Coverage function assigns wrong weights for gapped alignments
genomicalignments splicing coverage
updated 4.5 years ago by ★ 11k • written 7.1 years ago by ▴ 10
0
votes
4
replies
898
views
coverageByTranscript coudble counts overlapped region for paired-end data
GenomicAlignments RNA-seq paired-end read coverage
updated 4.7 years ago by 16k • written 4.7 years ago by ▴ 90
1
vote
9
replies
1.4k
views
stackStringsFromBam equivalent for GAlignments with seq
genomicalignments stackStringsFromBam sequenceLayer
4.8 years ago Janet Young ▴ 740
1
vote
8
replies
2.7k
views
How to use Rsamtool or GenomicAlignments to calculate RAN-seq read coverage curves
Rsamtools GenomicAlignments RNA-seq coverage score
updated 4.9 years ago by 16k • written 4.9 years ago by ▴ 90
4
votes
4
replies
1.2k
views
Mimic Counting of HTSeq-count Using countOverlaps
GenomicAlignments countOverlaps
updated 4.9 years ago by 25k • written 4.9 years ago by ★ 1.5k
0
votes
9
replies
1.4k
views
summarizeOverlaps bplapply error in DeSeq2 pipeline
summarizeOverlaps R GenomicAlignments
updated 4.9 years ago by 25k • written 4.9 years ago by • 0
3
votes
3
replies
998
views
How to tell a GAlignmentsList singleton is mate1 or mate2
Rsamtools GenomicAlignments GAlignmentsList
updated 5.1 years ago by 65k • written 5.1 years ago by ▴ 100
3
votes
2
replies
1.2k
views
Error in summarizeOverlaps
GenomicAlignments
updated 5.1 years ago by 65k • written 5.1 years ago by • 0
2
votes
1
reply
1.0k
views
Iterating over BAM file by genomic chunk
GenomicAlignments
updated 5.1 years ago by 25k • written 5.1 years ago by 65k
0
votes
2
replies
897
views
Duplicated reads if 'which' has overlapping reads in ScanBamParam
documentation Rsamtools GenomicAlignments
5.1 years ago Christian Mertes • 0
2
votes
3
replies
1.2k
views
indexBam(), summarizeOverlaps() error: RNA-seq
genomicAlignments RNA-seq Rsamtools
updated 5.2 years ago by ★ 6.5k • written 5.2 years ago by ▴ 70
1
vote
3
replies
1.1k
views
Efficient counting of nucleotide changes in short-reads
GenomicAlignments Biostrings
updated 5.4 years ago by ★ 11k • written 5.4 years ago by ★ 3.3k
0
votes
1
reply
1.2k
views
Subset bam file in R
rsamtools genomicalignments genomicranges
updated 5.4 years ago by ★ 11k • written 5.4 years ago by • 0
0
votes
2
replies
966
views
Preparation of summarizedExperiment for dexSeq
dexseq genomicalignments genomicfeatures genomicranges
5.5 years ago A ▴ 40
0
votes
2
replies
1.3k
views
Extracting fasta sequences from a RE digested genome .bed file
biostrings genomicalignments rsamtools
updated 5.7 years ago by 16k • written 5.7 years ago by • 0
1
vote
1
reply
1.3k
views
How to parse supplementary (not secondary) alignments
genomicalignments rsamtools
updated 5.7 years ago by 25k • written 5.7 years ago by ▴ 30
0
votes
1
reply
805
views
Scoring DNA motifs by conservation
conservation sequence genomicalignments
5.7 years ago martin.busch • 0
2
votes
2
replies
1.1k
views
How to write a preprocess.reads function for summarizeOverlaps that requires different logic for paired and single end reads?
summarizeoverlaps genomicalignments
updated 6.0 years ago by ★ 6.8k • written 6.0 years ago by ★ 7.9k
0
votes
0
replies
1.2k
views
large memory footprint with summarizeOverlaps method for BamViews
genomicalignments rsamtools BamViews summarizeoverlaps
updated 6.1 years ago by 25k • written 6.1 years ago by ▴ 10
1
vote
4
replies
1.5k
views
error displaying bam files
genomicalignments
6.4 years ago kamal.fartiyal84 ▴ 10
5
votes
8
replies
2.2k
views
How to set 'strandMode' with summarizeOverlaps()
GenomicAlignments
6.5 years ago Robert Castelo ★ 3.3k
2
votes
4
replies
1.5k
views
How to build a RNA-seq table of counts for repeatmasker annotations
GenomicFeatures repeatmasker GenomicAlignments
updated 6.5 years ago by ★ 11k • written 6.5 years ago by ★ 3.3k
2
votes
9
replies
2.1k
views
FDR adjustment in Time-Course DATA DESEQ2
deseq2 fdrtool genomicalignments
updated 6.5 years ago by 41k • written 6.6 years ago by ▴ 20
0
votes
0
replies
1.0k
views
Counting exonic reads with DEXSeq and GenomicAlignments
dexseq genomicalignments exon usage
6.6 years ago igor ▴ 40
5
votes
4
replies
2.4k
views
how to extract read alignments from reads aligning in mulitple locations
rsamtools genomicalignments genomicfeatures
updated 6.8 years ago by ★ 1.5k • written 6.8 years ago by ★ 3.3k
2
votes
5
replies
1.7k
views
NGS with summarizeOverlaps and SAM files
genomicalignments
updated 7.0 years ago by 65k • written 7.0 years ago by • 0
2
votes
1
reply
1.2k
views
GenomicAlignments/access correct bp positions near indels
genomicranges genomicalignments snp
updated 7.0 years ago by 16k • written 7.0 years ago by ▴ 90
3
votes
2
replies
1.4k
views
How are duplicates determined with readGAlignments/scanBam?
genomicalignments rsamtools
updated 7.2 years ago by ★ 6.5k • written 7.2 years ago by ▴ 30
0
votes
1
reply
1.1k
views
GenomicAlignment: Import genomic intervals described in a bed file into "summarizeOverlaps"
GenomicAlignments summarizeoverlaps
updated 7.2 years ago by 65k • written 7.2 years ago by ▴ 40
2
votes
2
replies
1.2k
views
Integer overflow when tabulating read coverage
genomicalignments genomicranges
updated 7.2 years ago by ★ 11k • written 7.2 years ago by ▴ 70
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Answer: Dominance Index for Shotgun Metagenomics Data by Leo Lahti ▴ 40
1. Note that Simpson's dominance index (\lambda) is a different index than inverse Simpson (1/\lambda) or reciprocal Simpson (1-\lambda). T…
Comment: Math expression in shapeCustom legend in EnhancedVolcano by pl23 • 0
Thank you very much - that worked perfectly! Word of warning to those who try this: it screws up the other legends because of the markdown …
Answer: PCA plot suggestions by Michael Love 41k
For help with interpretation and design of analysis, I recommend working with a local statistician. Due to time restrictions, I can only…
Comment: DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length by ussarizona ▴ 10
Hi Michael, Yes, I could figure it out what does the error mean. In my matrix I had the first colum GeneID while in my condition I only h…
Answer: Deseq2. decontXcounts not integers. Useful alternative? by Michael Love 41k
Another option would be to put the per sample estimated contamination in the design. This is for example how RUV etc help remove spurious D…
Votes
Answer: Dominance Index for Shotgun Metagenomics Data
Answer: Math expression in shapeCustom legend in EnhancedVolcano
Comment: DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
Comment: Deseq2. decontXcounts not integers. Useful alternative?
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
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