Showing : qPCR • reset
3
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500
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Creating qPCRset for nondetects anlaysis from QuantStudio data
nondetects qPCR qPCRData
3.0 years ago jhackett ▴ 30
3
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4
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3.0k
views
Using limma for the analysis of qPCR data
NormqPCR EasyqpcR qPCR HTqPCR limma
updated 2.5 years ago by ▴ 380 • written 3.3 years ago by ▴ 660
1
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2
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1.6k
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qRT-PCR Sample Maximization software analysis and proper experimental design advice
qPCR Sample Maximization Analysis Experimental Design qRT-PCR
updated 9.0 years ago by 50k • written 9.0 years ago by ▴ 450
1
vote
1
reply
1.8k
views
Are the logFC confidence intervals returned by topTable adjusted for multiple comparisons?
Microarray qPCR limma
updated 9.6 years ago by 50k • written 9.7 years ago by ▴ 30
1
vote
7
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1.6k
views
EDGE: Persistent lrt() and odp() command pi0est() error due to low p values
edge qpcr qvalue
7.2 years ago • updated 6.2 years ago joe.d.moore.ii • 0
1
vote
3
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2.4k
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ddCt calculation using bioconductor package
ddct qPCR
updated 2.9 years ago by ▴ 60 • written 9.1 years ago by ▴ 40
1
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0
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1.0k
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HTqPCR package data layout - how flexible is it?
qPCR qPCR
9.9 years ago Guest User ★ 13k
1
vote
4
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1.8k
views
ddCT Package for StepOnePlus qPCR output
qPCR ddCt geneexpression
updated 6.8 years ago by ▴ 120 • written 6.8 years ago by ▴ 60
1
vote
1
reply
1.2k
views
qRT-PCR - reading tab-delimited file (biol & tech replicates)
qPCR ReadqPCR qPCR ReadqPCR
11.3 years ago boczniak767 ▴ 720
1
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0
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1.8k
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DE analysis of PCR array [was: dataset dim for siggenes]
qPCR Normalization multtest limma siggenes
9.6 years ago • updated 7.3 years ago Gordon Smyth 50k
0
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3
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1.5k
views
RNASeq, differential expression between group, and large variance within groups
RNASeq qPCR edgeR DESeq RNASeq qPCR edgeR DESeq
updated 13.2 years ago by ★ 6.0k • written 13.2 years ago by 50k
0
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0
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735
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alternative CDF (Tarca, Adi)
qPCR cdf probe affy qPCR cdf probe affy
18.0 years ago raffaele calogero ▴ 500
0
votes
0
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937
views
4 replicates, of 3 tissues- one good hyb (2xreps), one bad hyb (2xreps). Best way forward?
qPCR affy affydata vsn limma gcrma matchprobes affyPLM affyio BRAIN qPCR affy affydata
17.7 years ago k. brand ▴ 420
0
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2
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949
views
how to 'bootstrap' expression data?
qPCR GO qPCR GO
updated 16.6 years ago by ★ 6.5k • written 16.6 years ago by ▴ 410
0
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1
reply
1.2k
views
R: Degradation plots - Affy package
Transcription qPCR Genetics probe affy Transcription qPCR Genetics probe affy
updated 17.7 years ago by ▴ 360 • written 17.7 years ago by ▴ 10
0
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4
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1.2k
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HTqPCR questions
qPCR HTqPCR qPCR HTqPCR
updated 13.3 years ago by ▴ 330 • written 13.3 years ago by ★ 2.0k
0
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2
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1.5k
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HTqPCR : readCtData problem?
miRNA qPCR HTqPCR miRNA qPCR HTqPCR
13.5 years ago Heidi Dvinge ★ 2.0k
0
votes
2
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1.9k
views
reading in data in ReadqPCR
qPCR Annotation HTqPCR qPCR Annotation HTqPCR
11.2 years ago Guest User ★ 13k
0
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0
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984
views
qPCR workshops in autumn 2008
qPCR qPCR
15.6 years ago qPCR Platform - the reference in real-t… ▴ 170
0
votes
3
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992
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HTqPCR and limma and factorial design with no replicates
Microarray qPCR Classification limma HTqPCR ASSIGN Microarray qPCR Classification limma
updated 14.3 years ago by ★ 6.0k • written 14.3 years ago by ▴ 810
0
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0
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902
views
Noch freie Workshop Plätze im Oktober 2008
qPCR qPCR
15.6 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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0
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892
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Noch freie qPCR Workshop Plätze im Oktober 2008
qPCR qPCR
15.6 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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0
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1.2k
views
Talk & Poster Call - qPCR 2009 Event
SNP qPCR Normalization Visualization Regression Cancer probe PROcess microRNA SNP qPCR
15.5 years ago qPCR Platform - the reference in real-t… ▴ 170
0
votes
1
reply
1.4k
views
Talk & Poster - Final Call - qPCR 2009 Event
SNP qPCR Normalization Visualization Regression Cancer probe PROcess microRNA SNP qPCR
15.4 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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0
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1.5k
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qPCR NEWS - December 2008
qPCR graph PROcess microRNA qPCR graph PROcess microRNA
15.4 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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3
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1.1k
views
Manual creation of Affybatch object to study highthrouput qPCR experiments.
miRNA qPCR affy miRNA qPCR affy
updated 12.6 years ago by ▴ 300 • written 12.6 years ago by ★ 13k
0
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0
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1.4k
views
qPCR NEWS - February 2009
qPCR Normalization PROcess microRNA qPCR Normalization PROcess microRNA
15.2 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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0
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967
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New microRNA & qPCR workshop in April 2009
SNP qPCR Normalization Regression microRNA SNP qPCR Normalization Regression microRNA
15.1 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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0
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625
views
High Throughput RTqPCR data
qPCR qPCR
14.8 years ago james perkins ▴ 300
0
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0
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945
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PLS help: is it sound to normalized highthrouput qPCR arrays using vsn?
qPCR Normalization vsn qPCR Normalization vsn
12.0 years ago Ali Mohammadian ▴ 290
0
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0
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1.6k
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HTqPCR
qPCR limma ddCt HTqPCR qPCR limma ddCt HTqPCR
11.6 years ago Heidi Dvinge ★ 2.0k
0
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0
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1.2k
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qPCR 2011 Symposium Talk and Poster CALL
qPCR Genetics microRNA qPCR Genetics microRNA
13.4 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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0
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1.6k
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qPCR event preview - April 2010
miRNA qPCR Normalization probe microRNA cycle miRNA qPCR Normalization probe microRNA
14.0 years ago qPCR Platform - the reference in real-t… ▴ 170
0
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0
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1.1k
views
qPCR plates containing multiple samples in HTqPCR
qPCR HTqPCR qPCR HTqPCR
14.2 years ago Heidi Dvinge ★ 2.0k
0
votes
1
reply
1.1k
views
HTqPCR and limma and factorial design with no replicates
Microarray qPCR Classification limma HTqPCR ASSIGN Microarray qPCR Classification limma
updated 14.3 years ago by ★ 6.0k • written 14.3 years ago by ▴ 810
0
votes
0
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1.2k
views
NEW deadline - Talk & Poster Call - qPCR 2009 Event
SNP qPCR Normalization Visualization Regression Cancer probe microRNA SNP qPCR Cancer
15.3 years ago qPCR Platform - the reference in real-t… ▴ 170
0
votes
4
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1.1k
views
HTqPCR only works for 384 qPCR plates
qPCR qPCR
updated 14.0 years ago by ★ 2.0k • written 14.0 years ago by ▴ 810
0
votes
9
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1.5k
views
htqPCR question
miRNA qPCR limma miRNA qPCR limma
updated 14.0 years ago by ▴ 810 • written 14.0 years ago by ★ 2.0k
0
votes
12
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1.8k
views
htqPCR question
miRNA qPCR Normalization limma miRNA qPCR Normalization limma
updated 14.0 years ago by ★ 2.0k • written 14.0 years ago by ▴ 810
0
votes
4
replies
1.5k
views
Reading in data with HTqPCR
qPCR Cancer cycle HTqPCR qPCR Cancer cycle HTqPCR
14.0 years ago • updated 13.9 years ago Heidi Dvinge ★ 2.0k
0
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0
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1.2k
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Experimental design for RNA-Seq
Sequencing qPCR Cancer Sequencing qPCR Cancer
13.9 years ago Jakob Hedegaard ▴ 170
0
votes
4
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2.4k
views
HTqPCR to analyze Fluidigm 96.96 Dynamic Array data
qPCR HTqPCR qPCR HTqPCR
updated 13.9 years ago by ★ 2.0k • written 13.9 years ago by ▴ 30
0
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2
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1.7k
views
Bacterial genomics
Sequencing Microarray qPCR genomes Sequencing Microarray qPCR genomes
updated 13.7 years ago by 16k • written 13.7 years ago by ▴ 30
0
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2
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1.0k
views
Advise on setting up a non-specific filter for differential expression
qPCR qPCR
13.7 years ago Lucia Peixoto ▴ 330
0
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2
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1.2k
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Advise on setting up a non-specific filter for differential expression
qPCR qPCR
13.7 years ago Tobias Straub ▴ 430
0
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1
reply
1.3k
views
gcrma vs rma
Microarray qPCR probe ROC limma gcrma Microarray qPCR probe ROC limma gcrma
updated 13.7 years ago by ▴ 260 • written 13.7 years ago by ▴ 330
0
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2
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1.3k
views
questions concerning quantile normalization in HTqPCR
miRNA qPCR miRNA qPCR
updated 13.6 years ago by ★ 2.0k • written 13.7 years ago by ▴ 810
0
votes
1
reply
938
views
normalization usinf the delta Ct method
qPCR Normalization qPCR Normalization
updated 13.7 years ago by ★ 13k • written 13.7 years ago by ▴ 810
0
votes
0
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1.2k
views
qPCR Event Calendar - autumn/winter 2010
qPCR Normalization Clustering probe microRNA qPCR Normalization Clustering probe microRNA
13.6 years ago qPCR Platform - the reference in real-t… ▴ 170
0
votes
0
replies
756
views
Any code for simulation of qPCR studies?
qPCR qPCR
12.2 years ago Ali Mohammadian ▴ 290
50 results • Page 1 of 1
Recent ...
Replies
Comment: package goseq seems to be not available on the latest version of R by Patrycja ▴ 20
limma package also isn't available.Is there any information when these packages will be available?
Comment: Why does GSEA on edgeR results for randomized samples give highly significant p- by Chris • 0
Thanks Gordon for the advice! I ran fgsea, gsva and camera(), the most significant pathways are different. For the purpose of finding pathw…
Comment: How to use bootRanges to bootstrap small RNA loci (nullranges package) by Michael Love 41k
Check the number of bootstrapped data per chromosome compared to the original, and then you can also look at its distribution. For this it'…
Comment: DNAString: Standard checksum function? by Henrik Bengtsson ★ 2.4k
For completeness: SEGUID v2 (https://www.seguid.org/), implemented in CRAN package **[seguid](https://cran.r-project.org/package=seguid)*…
Comment: fgsea significant result by Chris • 0
Thanks ATpoint! Setting a fixed seed can reproduce the same result. However, if someone else analyze the same data with different seed, the…
Votes
Answer: Why does GSEA on edgeR results for randomized samples give highly significant p-
Handling multiple differential expression comparisons
Answer: fgsea significant result
Answer: fgsea significant result
package goseq seems to be not available on the latest version of R
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