Showing : EdgeR • reset
1
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442
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Streamlining the computing time for MiloDE p-value correction in large dataset?
SingleCellExperiment edgeR miloR Bioconductor
updated 5 weeks ago by ★ 4.0k • written 10 weeks ago by • 0
0
votes
1
reply
218
views
How to model DE for RNA-seq in concordant monozygotic twins?
RNASeq variancePartition RUVSeq limma edgeR
updated 9 weeks ago by 53k • written 10 weeks ago by • 0
2
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2
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298
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replace cat with message in glmTreat when lfc = 0
edgeR glmTreat
updated 12 weeks ago by 53k • written 12 weeks ago by • 0
0
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4
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431
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voomLmFit fitted log-CPM values
limma edgeR
updated 3 months ago by 53k • written 3 months ago by ▴ 10
2
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9
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989
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Transcript expression analysis in edgeR from gtf file obtained from merging long read rna-seq and standard gencode gtf
RNASeq edgeR LongRead Transcript
4 months ago mohammedtoufiq91 ▴ 10
3
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2
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906
views
Transcript expression analysis using EdgeR
edgeR Transcript RNASeq
4 months ago mohammedtoufiq91 ▴ 10
0
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1
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945
views
Using offset in edgeR
edgeR
updated 4 months ago by 53k • written 4 months ago by • 0
4
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8
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1.9k
views
Should Xenium pseudobulk experiments be analysed like microarrays (limma) or like RNASeq (edgeR,Deseq2, or limma-voom)
Deseq2 edgeR limma xenium Voom
5 months ago richardallenfriedmanbrooklyn ▴ 30
7
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9
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5.6k
views
competitive enrichment test yields too few significant results, self-contained too many
limma DESeq2 fry edgeR
5 months ago bbao ▴ 10
5
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11
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3.3k
views
parameters of filterByExpr
edgeR DESeq2 filterByExpr
written 7 months ago by ▴ 10
2
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4
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2.7k
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Altered (negative or positive) logFC values for same gene from two intitial count matrices, one being a subset of the other
Transcriptomics edgeR DEG
6 months ago Maziya • 0
2
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3
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1.5k
views
Differential gene expression analysis on haplotype-resolved diploid assembly
edgeR DESeq2 haplotype limma
6 months ago Paulito • 0
0
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2
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888
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edgeR in DMR mode - replicates give batch effect - how to build design matrix with condition and replicate
edgeR
updated 6 months ago by ▴ 110 • written 6 months ago by • 0
0
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3
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1.0k
views
Model performance for spline modelling of RNA-seq timecourses in edgeR
edgeR limma
written 8 months ago by ▴ 10
10
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13
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2.7k
views
edgeR::cpm() results in negative values from raw counts of 0
edgeR
updated 7 months ago by 53k • written 7 months ago by ▴ 20
2
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2
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1.5k
views
Improving performance of edgeR and limma while adjusting for multiple confounders
edgeR limma
updated 8 months ago by ★ 5.0k • written 8 months ago by • 0
5
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3
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1.2k
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Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with multiple cell types
edgeR limma voomLmFit
updated 8 months ago by 53k • written 8 months ago by ▴ 10
2
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2
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1.6k
views
Limma - Complex 2-way multi-level contrast with paired samples
edgeR contrastmatrix limma
9 months ago Elizabeth • 0
0
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2
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750
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Removing UTR from GTF for gene quantification
edgeR DESeq2
updated 9 months ago by 43k • written 9 months ago by ▴ 20
0
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1
reply
453
views
Is it possible to model DNA methylation as a dose response in edgeR?
edgeR
updated 9 months ago by 53k • written 9 months ago by • 0
0
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4
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1.4k
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DiffBind edgeR normalization --- why are the normalization factors all equal to 1?
edgeR DiffBind
updated 9 months ago by • 0 • written 9 months ago by • 0
2
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4
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925
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EdgeR Biological CVs
edgeR
written 10 months ago by • 0
3
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5
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2.1k
views
Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
limma edgeR biomaRt
updated 11 months ago by 68k • written 11 months ago by ▴ 10
0
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2
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721
views
Mean variance curve troubleshoot filtering
limmavoom filtering edgeR RNASeqData meanvariancetrend
11 months ago gingergeiger22 • 0
0
votes
1
reply
607
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR DESeq2
updated 11 months ago by 43k • written 11 months ago by • 0
1
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8
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1.6k
views
Incorporating Salmon offset matrix into Wilcoxon DEG analysis
edgeR
11 months ago Andre • 0
1
vote
9
replies
1.5k
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma DESeq2 edgeR
written 12 months ago by ▴ 40
6
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10
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3.4k
views
What type of counts data to import for performing Isoform analysis in edgeR
limma isoform edgeR salmon R
updated 12 months ago by ★ 5.0k • written 12 months ago by ▴ 10
7
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6
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1.4k
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ANOVA like approach of edgeR
edgeR
updated 12 months ago by 53k • written 12 months ago by ▴ 10
3
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3
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947
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Is it recommended to analyze different tissues together when using edgeR
DifferentialExpression edgeR
updated 12 months ago by 53k • written 12 months ago by • 0
2
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4
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2.5k
views
Differential Expression Analysis with unbalanced batches without replication between conditions
edgeR BatchEffect
updated 13 months ago by 53k • written 13 months ago by • 0
1
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8
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2.5k
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Combining RNAseq and microarray data using edgeR/limma-voom
limma voom RNASeq edgeR Microarray
written 13 months ago by • 0
0
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1
reply
906
views
single cell RNA seq pseudobulk batch correction
edgeR
updated 13 months ago by 68k • written 13 months ago by • 0
3
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3
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1.5k
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Robust way of dealing with low number of samples for Differential Gene Expression
limma edgeR DESeq2 MAST
written 13 months ago by • 0
0
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4
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1.1k
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs edgeR RNASeqData
13 months ago Philippine • 0
4
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6
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1.9k
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Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma duplicateCorrelation edgeR modelMatrixMeth voomLmFit
14 months ago • updated 13 months ago altintas.ali • 0
1
vote
2
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742
views
edgeR 4.2.2 not compiled for Windows
edger edgeR
14 months ago Hongjian • 0
3
votes
3
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774
views
How to subset voomLmFit (MArrayLM) object?
limma edgeR
14 months ago Guido Hooiveld ★ 4.1k
4
votes
1
reply
681
views
edgeR 4.2.1 compression bug: a minimally reproducible snippet
edger edgeR
updated 14 months ago by 53k • written 14 months ago by • 0
1
vote
1
reply
707
views
Normalization RNA-seq for multiple plates
Normalization DESeq2 RNASeqData edgeR
updated 14 months ago by ★ 5.0k • written 14 months ago by • 0
1
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2
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907
views
Poor dispersion fit in DESeq2
limma DESeq2 RNASeq edgeR
updated 14 months ago by 43k • written 14 months ago by ▴ 10
0
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2
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814
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma edgeR
updated 14 months ago by 53k • written 14 months ago by • 0
1
vote
2
replies
710
views
Inconsistent result in edgeR
edgeR
15 months ago hiroko.ohmiya • 0
1
vote
1
reply
870
views
Help with design matrix for RNA-seq data using edgeR
edgeR RNASeqData
updated 15 months ago by 53k • written 15 months ago by • 0
2
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9
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1.5k
views
Seeking Advice on Using edgeR and variancePartition for RNA-seq Data from Multiple Tissues and Patients
edgeR variancePartition
updated 15 months ago by 53k • written 15 months ago by • 0
0
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3
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1.0k
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2 edgeR DifferentialExpression
written 16 months ago by • 0
2
votes
1
reply
2.2k
views
Expected memory usage for analyzing large single-cell seq datasets (> 450,000 cells)?
BigData Clustering memory SingleCellExperiment edgeR
updated 16 months ago by ★ 29k • written 16 months ago by • 0
0
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1
reply
791
views
edgeR - design a complex matrix
design model.matrix edgeR complexdesign
updated 16 months ago by 53k • written 16 months ago by • 0
3
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3
replies
1.1k
views
Optimizing CPM Calculation for Varied RNA-seq Data
edgeR
updated 16 months ago by 68k • written 16 months ago by ▴ 60
0
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4
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1.1k
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData DEGraph RNASeq edgeR DegNorm
written 17 months ago by • 0
2,031 results • Page 1 of 41
Recent ...
Replies
Comment: Bioinformatician at the Lausanne University Hospital by eja.ger.m • 0
For individuals looking to improve communication and collaboration in demanding medical or research environments like the Genetic Medicine …
Answer: NUPOP usage by shepherl 4.2k
You should read the documentation in the package especially the package vignette https://bioconductor.org/packages/release/bioc/html/NuPoP.…
Comment: How can I correctly use phyloseq with Docker? by fnfgame • 0
In a rocker image, this error usually means the Bioconductor install happened as root but the runtime library paths used by the shiny user …
Comment: DECIPHER DesignProbes unable to design FISH-probes by Erik Wright ▴ 150
Are the sequences aligned (i.e., the same width) before running `TileSeqs()`?
Comment: BiocParallel (and DESeq2) - wrong args for environment subassignment by Michael Love 43k
BTW, I tested with no issue on MacOS ARM on our previous github issue.
Votes
C: DESeq2 Following RSEM
Upcoming Ensembl API and data access changes - new blog post available
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Large logFC but somewhat high FDR
Comment: upstream git branches of bioc-release
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