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limma
•
reset
1
vote
2
replies
255
views
Multi-factorial, longitudinal disease progression analysis with unbalanced patient sampling - duplicateCorrelation or blocking?
rna-seq
duplicateCorrelation
limma
voom
9 days ago • updated 8 days ago
Dylan.Sheerin
• 0
2
votes
2
replies
269
views
error in limma , contrast.matrix Number of rows of contrast matrix must match number of coefficients in fit
limma
10 days ago • updated 4 days ago
zzrammal
• 0
3
votes
10
replies
441
views
Extremely small p-values using Limma for proteomic data
Proteomics
limma
updated 7 days ago by
James W. MacDonald
65k • written 10 days ago by
Abbey
• 0
1
vote
2
replies
398
views
limma Intercept vs No-intercept models completely changing DMR results?
limma
DMRcate
MethylationArray
EPICV2
updated 14 days ago by
Gordon Smyth
50k • written 14 days ago by
Kim
• 0
0
votes
1
reply
154
views
Gene-by-sample covariates in limma
Metabolomics
limma
updated 15 days ago by
Gordon Smyth
50k • written 15 days ago by
c53aba27
• 0
3
votes
4
replies
260
views
Help with error using camera() in limma
CAMERA
limma
17 days ago
Chris
▴ 10
0
votes
3
replies
273
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
updated 17 days ago by
James W. MacDonald
65k • written 18 days ago by
SSSJec
• 0
0
votes
2
replies
190
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 25 days ago by
taur.vil
• 0
0
votes
2
replies
237
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
28 days ago • updated 25 days ago
catv
• 0
15
votes
17
replies
1.3k
views
Why p value inconsistent when using different number of pathway score?
limma
27 days ago • updated 16 days ago
Chris
▴ 10
5
votes
3
replies
304
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 8 days ago by
Gordon Smyth
50k • written 29 days ago by
Shaimaa Gamal
• 0
0
votes
7
replies
362
views
Methylation Analysis experiment design using Limma
DNAMethylation
limma
methylationArrayAnalysis
4 weeks ago • updated 24 days ago
hortense96
• 0
1
vote
3
replies
368
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 4 weeks ago by
Gordon Smyth
50k • written 5 weeks ago by
Simran
• 0
5
votes
9
replies
974
views
Light difference when using coef vs omit a group when compare 3 groups.
limma
5 weeks ago • updated 29 days ago
Chris
▴ 10
2
votes
6
replies
459
views
How to make boxplot with pairwise and overall p value from Limma
t.test
boxplot
limma
anova
5 weeks ago
Chris
▴ 10
0
votes
1
reply
185
views
Limma design matrix only showing 3/4 treatments?
limma
designmatrix
updated 5 weeks ago by
Gordon Smyth
50k • written 6 weeks ago by
avery
• 0
0
votes
4
replies
330
views
Help with design.matrix
limma
6 weeks ago • updated 5 weeks ago
andrea.rodriguez-martinez13
• 0
1
vote
4
replies
462
views
Using a change in a continuous explanatory variable to test changes in gene expression with limma
limma
updated 6 weeks ago by
Gordon Smyth
50k • written 6 weeks ago by
jari.karppinen
• 0
0
votes
2
replies
207
views
voom(calcNormFactors()) for normalization of RNAseq counts for WGCNA
Voom
Limma
WGCNA
6 weeks ago
Gimena
• 0
15
votes
23
replies
2.0k
views
The difference when using Limma compare between 3 conditions vs 2 conditions
limma
7 weeks ago • updated 17 days ago
Chris
▴ 10
2
votes
8
replies
560
views
Microarray Limma Model fitting and DEG
limma
DGEanalysis
updated 7 weeks ago by
Gordon Smyth
50k • written 7 weeks ago by
Amit
• 0
5
votes
25
replies
986
views
limma models out of memory issue on Slurm HPC
Regression
limma
STATISITICS
HPC
updated 6 weeks ago by
Gordon Smyth
50k • written 7 weeks ago by
Jitendra
▴ 10
7
votes
6
replies
481
views
Batch Effect Correction, Gene Filtering
DGE
limma
updated 8 weeks ago by
Gordon Smyth
50k • written 8 weeks ago by
Amit
• 0
0
votes
1
reply
250
views
Using splicing junction counts with edgeR/limma-voom
splicing
limma
edgeR
updated 8 weeks ago by
Gordon Smyth
50k • written 8 weeks ago by
scoops_streams_06
• 0
0
votes
4
replies
410
views
Unable to annotate ExpressionSet object from hugene11st chip
oligo
limma
hugene11stprobeset.db
AffymetrixChip
8 weeks ago • updated 7 weeks ago
Efra
• 0
5
votes
7
replies
484
views
DEG Filtering
MicroarrayData
limma
Genefiltering
updated 7 weeks ago by
Gordon Smyth
50k • written 8 weeks ago by
Amit
• 0
2
votes
2
replies
289
views
Question about extracting co-efficients for pairwise comparisons in edgeR
limma
fit
design
RNASeq
edgeR
9 weeks ago
Sabiha
▴ 20
0
votes
2
replies
313
views
RNA sequencing raw counts gene filtering
DESeq2
limma
RNAseq
9 weeks ago
kcarey
• 0
1
vote
3
replies
439
views
Cross-species analysis - how to compute the mid parent value?
DESeq2
limma
edgeR
DifferentialExpression
written 3 months ago by
pl23
• 0
2
votes
6
replies
556
views
limma: vooma mean-variance trend and data filtration
MicroarrayData
limma
10 weeks ago • updated 7 weeks ago
Lily Cheang
▴ 10
3
votes
8
replies
540
views
Constrast for Time Course Experiments using Limma
limma
10 weeks ago
dl17032000
• 0
8
votes
6
replies
502
views
DGE analysis with LIMMA
limma
updated 11 weeks ago by
Gordon Smyth
50k • written 11 weeks ago by
Amit
• 0
0
votes
0
replies
165
views
Can I combine collinear variables into a single value
BatchEffect
limma
11 weeks ago
Harrsha
• 0
0
votes
3
replies
318
views
Differential enrichment analysis
limma
Differentialenrichmentanalysis
DEP
updated 10 weeks ago by
Gordon Smyth
50k • written 11 weeks ago by
Laura
• 0
0
votes
4
replies
323
views
Interactions in batches of varying size with different number of replicates using limma
limma
RNASeq
DifferentialExpression
11 weeks ago
mperalc
• 0
3
votes
5
replies
668
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 10 weeks ago by
Michael Love
42k • written 12 weeks ago by
sap275
▴ 10
2
votes
1
reply
278
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 11 weeks ago by
Michael Love
42k • written 11 weeks ago by
sap275
▴ 10
1
vote
2
replies
338
views
Bioconductor unreachable?
limma
12 weeks ago
edoardo
• 0
0
votes
9
replies
589
views
limma fo continous variables
limma
DEGseq
updated 11 weeks ago by
James W. MacDonald
65k • written 12 weeks ago by
mia
• 0
0
votes
2
replies
318
views
limma model matrix questions
limmaGUI
limma
pairedsamples
12 weeks ago
whyw6948
• 0
0
votes
2
replies
347
views
Limma with replicates at different time points
RNAs
limma
Differen
3 months ago • updated 11 weeks ago
alessandro
• 0
2
votes
1
reply
320
views
Normalization factors when there is a possible unidirectional expression
limma
Normalization
DifferentialExpression
updated 3 months ago by
ATpoint
★ 4.1k • written 3 months ago by
Shaimaa Gamal
• 0
1
vote
3
replies
386
views
CAMERA + Gene Ontology Gene Sets
GO
limma
CAMERA
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
Tyler Sagendorf
▴ 10
0
votes
4
replies
393
views
limma with interaction term
R
biostatistics
limma
Epigenetics
3 months ago • updated 11 weeks ago
Jitendra
▴ 10
3
votes
2
replies
317
views
Design matrix after RUVg correction
RUVSeq
limma
DifferentialExpression
3 months ago
Shaimaa Gamal
• 0
2
votes
3
replies
348
views
Longitudinal analysis in Limma: Block=ID as random effect
limma
3 months ago
COPD_Project
• 0
2
votes
2
replies
305
views
Limma design for 3 replicated time courses comparing treatment vs placebo
design
limma
3 months ago
SamGG
▴ 350
1
vote
4
replies
441
views
limma design model for categorical variable
DNAMethylation
limmaGUI
biostatistics
limma
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
Jitendra
▴ 10
4
votes
3
replies
378
views
voom with quality weights after RUV correction
RUVSeq
limma
DifferentialExpression
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
Shaimaa Gamal
• 0
2
votes
3
replies
386
views
TMM normalization after RUVg correction
RUVSeq
limma
DifferentialExpression
edgeR
Normalization
updated 3 months ago by
James W. MacDonald
65k • written 3 months ago by
Shaimaa Gamal
• 0
50 results • Page
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Replies
Comment: Numerical differences in DESeq2 output depending on different design matrix cons
by
JKim
• 0
The 1st model has an interaction term, but the 2nd model doesn't have the interaction term. That makes the difference.
Comment: Ensembl plants at annotationHub?
by
Daniele
• 0
Hi, I'm using Ensembl Plants release 59 with the IWGSC RefSeq v1.1 gene annotation. Thanks a lot for replying so quickly!
Comment: Numerical differences in DESeq2 output depending on different design matrix cons
by
swbarnes2
★ 1.4k
If you restrict yourself to, say, genes with a baseMean > 50, are the numerical differences between the two designs significant?
Comment: DESeq2- Mysterious interaction suppresses DEGs
by
shaunpeterson
• 0
The images I tried to attach didn't attach. Here is the PCA (MDS) of all the samples, with the Cortex outlier highlighed. ![enter image de…
Comment: STRINGdb : error with string_db$plot_network()
by
damian.szk
▴ 80
ok. Forcing non-secure protocol should not be needed anymore, due to changes in the underlying library. However, I will look at why "HTTP" …
Votes
Answer: Error while installing BiocManager::install("org.Vdahliae.eg.db")
Comment: How can I create a eigencorplot of pca created from deseq2 object with PCAtools
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Rsubread counting peaks ATACseq
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