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MethylationArray
•
reset
5
votes
5
replies
309
views
limma output for epic array methylation data
linearregression
epicarray
MethylationArray
limma
5 weeks ago
Jitendra
▴ 10
1
vote
1
reply
309
views
urgent error for producing table of topGO() annotated DMPs methylation
methylationArrayAnalysis
topGO
missMethyl
MethylationArrayData
MethylationArray
updated 4 months ago by
belinda.phipson
▴ 40 • written 4 months ago by
Grace
• 0
2
votes
6
replies
577
views
cpg.annotate() in DMRcate error
DNAMethylation
methylationArrayAnalysis
DMRcate
MethylationArrayData
MethylationArray
updated 5 months ago by
Tim Peters
▴ 140 • written 5 months ago by
Grace
• 0
0
votes
1
reply
225
views
LmFit error in EPIC methylation array analysis, Minfi
methylationArrayAnalysis
minfi
MethylationArrayData
minfiDataEPIC
MethylationArray
updated 5 months ago by
Gordon Smyth
48k • written 5 months ago by
Grace
• 0
0
votes
4
replies
463
views
Sex estimation problems in [estimateCellCounts]: methylation arrays
methylationArrayAnalysis
minfiData
minfi
MethylationArrayData
MethylationArray
updated 6 months ago by
James W. MacDonald
63k • written 6 months ago by
Grace
• 0
0
votes
5
replies
486
views
minfi error read.metharray.exp
methylationArrayAnalysis
minfiData
minfi
MethylationArrayData
MethylationArray
written 6 months ago by
Grace
• 0
2
votes
1
reply
461
views
Limma model orientation
limma
methylation
MethylationArray
updated 16 months ago by
James W. MacDonald
63k • written 17 months ago by
fluentin44
• 0
2
votes
2
replies
829
views
Limma vs. lm for methylation data
limma
MethylationArrayData
MethylationArray
17 months ago
fluentin44
• 0
0
votes
0
replies
747
views
Surrogate variable, celltype composition correction and overfitting in DNA methylation data
sva
limma
methylation
MethylationArray
2.7 years ago
epibio
▴ 10
6
votes
6
replies
1.2k
views
Limma Paired Analysis - Force intercept to 0
MethylationArray
limma
2.7 years ago • updated 2.6 years ago
MoltenLight
▴ 10
2
votes
6
replies
1.9k
views
Blood cell composition adjustment in methylation EPIC array: ChAMP
MethylationArray
methylationArrayAnalysis
ChAMP
updated 18 months ago by
l.milicic
• 0 • written 2.8 years ago by
Ankit
▴ 20
0
votes
0
replies
477
views
Diffrential Gene expression in a group of specific methylated prometor patience
GeneExpression
methyAnalysis
MethylationArray
2.9 years ago
coobzie
• 0
12 results • Page
1 of 1
Recent ...
Replies
Answer: Batch effect adjust in model matrix
by
ATpoint
★ 3.4k
Each sample is a different day, that is called "nested", so there is nothing you can do here. Next time do a better experimental design, he…
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Michael Love
40k
Oh, I see. If you can't use counts + offset then I prefer lengthScaledTPM to DESeq2, i.e. `txi$counts` to `DESeqDataSetFromMatrix()`. So …
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Christian
• 0
I´m sorry causing confusion here, but the gene matrices I refer to are actually obtained via https://nf-co.re/rnaseq. The problem with the…
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Michael Love
40k
For the reasons outlined in the Soneson paper, I'd prefer this pipeline: https://nf-co.re/rnaseq You cannot use these scaled TPM approach…
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Christian
• 0
I get your point as we already starting with TPM. This was more to clarify that I understand the principle correctly. However, further I´m…
Votes
batch effect : comBat or blocking in limma ?
Batch correction and repeated measures
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
Comment: Phenotype effect - building a contrast matrix - grouping vs interaction
Answer: Batch correction and repeated measures
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Gordon Smyth
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