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DESEq2
•
reset
6
votes
9
replies
5.9k
views
Centering and scaling numeric variables
deseq2
updated 5.7 years ago by
adR
▴ 40 • written 5.7 years ago by
MiKappa
▴ 30
0
votes
4
replies
735
views
How to know what genes are differentially expression in DESeq2 simulation?
deseq2
updated 5.7 years ago by
Kevin Blighe
★ 4.0k • written 5.7 years ago by
megapode32559
• 0
0
votes
1
reply
374
views
Choosing DESeq2 design
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
zen
• 0
1
vote
3
replies
1.2k
views
DE analysis outlier removal
deseq2
outlier
normalization
removal
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
knholm
• 0
0
votes
1
reply
863
views
DESeq dispersion parameter
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
stephen.hartley
• 0
0
votes
2
replies
1.0k
views
phyloseq_to_deseq2 design strategy for multiple factors analysis (2 or more)
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
pyveronneau71
• 0
5
votes
2
replies
1.7k
views
DESeq2 pairwise comparisons w/ 3 factors
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
aharkey
▴ 10
0
votes
6
replies
1.7k
views
Remove batch effect for DESeq2
deseq2
updated 5.7 years ago by
James W. MacDonald
68k • written 5.7 years ago by
luongthang1908
• 0
0
votes
3
replies
2.1k
views
DESeq2 - cannot find function?
deseq2
r
software error
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
lindsay
• 0
4
votes
3
replies
1.2k
views
RNA-Seq analysis with DESeq2: showing two conditions do *not* significantly differ
deseq2
RNA-Seq
uniform distribution
data visualization
updated 5.7 years ago by
Steve Lianoglou
★ 13k • written 5.7 years ago by
Ndimensional
▴ 20
0
votes
1
reply
358
views
DESeq2 : Model clarification
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
Nicolas Rosewick
▴ 20
0
votes
8
replies
3.4k
views
DESeq LRT reduced model
deseq2
LRT
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
bio_inc
• 0
0
votes
5
replies
1.2k
views
Negative Binomial model for microbiome data
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
wisam.tariqsaleem
• 0
1
vote
1
reply
7.1k
views
How to import featureCounts data into DESeq2
DESEq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
chunter
• 0
0
votes
1
reply
1.4k
views
Adjusting for unwanted variation in DESeq2
DESeq2
deseq2
sva
rna-seq
differential expression anlaysis
updated 5.7 years ago by
James W. MacDonald
68k • written 5.7 years ago by
Nikolay Ivanov
• 0
1
vote
1
reply
1.1k
views
multifactor desing with interaction LRT or Wald test?
deseq2
LRT
wald
updated 5.7 years ago by
Kevin Blighe
★ 4.0k • written 5.7 years ago by
jnaviapelaez
▴ 10
0
votes
1
reply
790
views
Meaning of log2 fold change in 2 x 4 interaction
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
julia.chariker
▴ 10
1
vote
3
replies
2.2k
views
In DESeq2, how do you interpret results based on the order of variables in the "contrast" argument?
deseq2
updated 5.7 years ago by
Kevin Blighe
★ 4.0k • written 5.7 years ago by
gpreising
• 0
1
vote
1
reply
564
views
mate pairs read counts as input for DESeq2
deseq2
updated 5.7 years ago by
James W. MacDonald
68k • written 5.7 years ago by
jnaviapelaez
▴ 10
1
vote
2
replies
1.1k
views
Data not getting normalised after DESeq2
deseq2
normalization
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
tg369
▴ 40
3
votes
6
replies
961
views
MA plot shows inflation of downregulated genes.
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
spr
▴ 10
0
votes
1
reply
3.3k
views
Where in the procedure do I remove low-expressed genes?
deseq2
edger
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
marcha.verheijen
• 0
0
votes
3
replies
1.3k
views
DESeq2 accounting for two confounders with interaction in the design matrix
deseq2
design matrix
multi-confounders
linear models
rna-seq
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
mohamedrefaat.1.1992
• 0
1
vote
3
replies
1.4k
views
DESeq2 - controlling for other variables
deseq2
DEG
covariate
study design
variables
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
Yonatan Amzaleg
• 0
1
vote
1
reply
484
views
DESeq2: Identify similarly expressed genes
deseq2
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
wangy660
• 0
0
votes
8
replies
1.4k
views
DESeq2 notice about GLM convergence
deseq2
RNASeq
updated 5.7 years ago by
Michael Love
43k • written 5.7 years ago by
stephen.hartley
• 0
3
votes
3
replies
2.0k
views
Repeated error loading DESqe2 - package xxx not found
deseq2
package
software error
updated 5.7 years ago by
Martin Morgan
25k • written 5.8 years ago by
DcL-A
• 0
0
votes
6
replies
2.1k
views
Bizarre Dispersion Plots
deseq2
RNA
RNASeq
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
stephen.hartley
• 0
2
votes
2
replies
1.3k
views
RNA-seq analysis with DESeq2
deseq2
updated 5.8 years ago by
mikhael.manurung
▴ 280 • written 5.8 years ago by
alexandr.gopanenko
▴ 50
2
votes
8
replies
1.5k
views
DESEQ2 design with unequal matching and replicates?
deseq2
RNASeq
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
divyak
• 0
2
votes
3
replies
730
views
The basis for the full rank design matrix requirement in DESeq2
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
Nik Tuzov
▴ 90
0
votes
2
replies
735
views
DESeq2 error on function results
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
jrossol
• 0
0
votes
1
reply
1.4k
views
What does an asymmetrical MA plot indicate and how do I know if it means I've made an error?
deseq2
plotMA
rnaseq
community analysis
asymmetry
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
chaco001
• 0
2
votes
3
replies
724
views
Order of levels within factors affects DESeq2 results when interaction is included
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
dfab
• 0
1
vote
5
replies
732
views
subtle differences in results based on how Design Table is coded
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
germaximus
• 0
0
votes
0
replies
922
views
News:
RNAseq data analysis with R and Bioconductor
limma
deseq2
go
edger
News
5.8 years ago
info
▴ 10
0
votes
3
replies
1.2k
views
how to interpret results from 3way design in DESeq2
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
demisa
• 0
0
votes
3
replies
1.6k
views
Highly variable gene analysis including RUVs batch correction
deseq2
RUV
batch
highly variable genes
updated 5.8 years ago by
tmms
• 0 • written 5.8 years ago by
f_pardow
• 0
4
votes
5
replies
1.7k
views
Difference in dimensionality reduction plots based on DESeq2 R package and core functions for RNA-Seq data
deseq2
vst
pca
cpm
5.8 years ago
svlachavas
▴ 840
2
votes
8
replies
3.4k
views
DESeq2 : log2FC discrepencies between results and lfcShrink
deseq2
shrinkage
updated 3.0 years ago by
Michael Love
43k • written 5.8 years ago by
Nicolas Rosewick
▴ 20
2
votes
4
replies
1.6k
views
DESeq2 - problem with inferring correct results.
deseq2
rnaseq
sva
fdrtool
ihw
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
rihards.saksis
• 0
0
votes
7
replies
1.7k
views
tximeta doesn't work downloading the ref genome
deseq2
tximeta
tximport
rnaseq
5.8 years ago
rafaelsolersanblas
▴ 50
1
vote
2
replies
1.8k
views
For loop for DESeq2
loop
deseq2
rnaseq
updated 3.4 years ago by
chm
• 0 • written 5.8 years ago by
NeuroGeneticsBoy
▴ 10
0
votes
1
reply
599
views
DESeq2: basemean count
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
Brian Smith
▴ 120
0
votes
1
reply
509
views
DESeq2: multiple factors, comparison of a main factor between samples with inconsistent magnitudes of gene expression changes
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
jropa
▴ 10
0
votes
1
reply
1.1k
views
DESeq2 with uneven group size
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
Zohaib Anwar
• 0
0
votes
1
reply
2.5k
views
RLE plot of DESeq2 normalisation / extract matrix of normalised counts from DESeqDataSet
deseq2
RLE
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
f_pardow
• 0
1
vote
3
replies
1.1k
views
DESeq2 : Estimating dispersions takes forever to finish
deseq2
5.8 years ago
Nicolas Rosewick
▴ 20
3
votes
5
replies
1.8k
views
Emirical Bayesian shrinkage for dispertion/LFC estimation
rnaseq
deseq2
updated 5.8 years ago by
Michael Love
43k • written 5.8 years ago by
sotogarcia.r
▴ 10
1
vote
2
replies
582
views
I have four groups in my RNAseq experiment and want to compare them all to each other (not them all to a control). How do I do this is deseq2?
deseq2
updated 5.8 years ago by
swbarnes2
★ 1.4k • written 5.8 years ago by
michael_sportiello
• 0
4,840 results • Page
42 of 97
Recent ...
Replies
Comment: Question about DESeq2 Design for Paired Treatment Study
by
ATpoint
★ 5.0k
It's a paired analysis so you don't need any patient-specific covariates such as age and sex. What you call batch effect might just be the …
Comment: problem reading bam files into nucleR
by
efoss
▴ 10
I thought I might be able to track down a problematic line in the bam file by repeatedly splitting the bam file in and then running the co…
Answer: two questions about limma
by
Gordon Smyth
53k
Answered here: https://support.bioconductor.org/p/16318/
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
by
Ahmed Salah
• 0
Thank you very much! will take that into consideration
Comment: Large logFC but somewhat high FDR
by
JKim
• 0
Thank you very much for taking the time to answer the question. I really appreciate your insights and comments.
Votes
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Large logFC but somewhat high FDR
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
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