Showing : DESeq2 • reset
0
votes
6
replies
159
views
Once again a "Model matrix not full rank"
DESeq2 design coldata
updated 12 hours ago by ★ 1.3k • written 1 day ago by ▴ 130
2
votes
2
replies
103
views
Too many significant genes when integrating gtex and tcga
RNA-seq DESeq2
updated 23 hours ago by 41k • written 23 hours ago by • 0
0
votes
0
replies
35
views
Handling multiple differential expression comparisons
DESeq2
1 day ago Zainab • 0
0
votes
2
replies
78
views
DESeq2 v1.35.0
DESeq2
updated 2 days ago by 41k • written 2 days ago by • 0
0
votes
2
replies
111
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
2 days ago • updated 1 day ago kcarey • 0
0
votes
3
replies
112
views
Differences in differential analysis methods for RNA-Seq
DESeq2 StatisticalMethod Proteomics DifferentialExpression RNASeq
updated 2 days ago by ★ 4.1k • written 2 days ago by • 0
0
votes
1
reply
121
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 2 days ago by ★ 4.1k • written 2 days ago by • 0
0
votes
3
replies
233
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 2 days ago by 41k • written 3 days ago by • 0
1
vote
1
reply
94
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind ChIPSeqData DESeq2
updated 4 days ago by 41k • written 4 days ago by • 0
0
votes
2
replies
109
views
Timecourse RNASeq analysis
DESeq2 ImpulseDE2 timecour timecoursedata moanin
4 days ago • updated 1 day ago Aurora • 0
0
votes
1
reply
93
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 4 days ago by 41k • written 4 days ago by • 0
0
votes
1
reply
94
views
putting my own expression data in deseq2
DESeq2
updated 4 days ago by ★ 4.1k • written 4 days ago by • 0
3
votes
3
replies
207
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2 contrasts
updated 5 days ago by ★ 4.1k • written 5 days ago by ▴ 10
0
votes
2
replies
193
views
PCA plot suggestions
DESeq2 PCAtools
updated 8 days ago by 41k • written 9 days ago by • 0
1
vote
3
replies
203
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2 celda
updated 8 days ago by 41k • written 8 days ago by • 0
2
votes
9
replies
363
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2 RUVSeq RUVseq BatchEffect DES
updated 8 days ago by 65k • written 9 days ago by • 0
0
votes
1
reply
143
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2 RNAseq
updated 9 days ago by 41k • written 10 days ago by • 0
1
vote
3
replies
205
views
Different Shrunk Log2FC according with reference
DESeq2
updated 10 days ago by 41k • written 10 days ago by ▴ 20
1
vote
4
replies
313
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
10 days ago • updated 8 days ago winwater0928 • 0
3
votes
1
reply
303
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 10 days ago by 41k • written 11 days ago by ▴ 40
0
votes
8
replies
340
views
DESeq2 output used for PCA plot on R studio
DESeq2 RSTUDIO PCAtools
updated 9 days ago by ★ 1.3k • written 11 days ago by • 0
3
votes
3
replies
220
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 11 days ago by 41k • written 12 days ago by • 0
0
votes
1
reply
132
views
rlog transformation
DESeq2
updated 16 days ago by 41k • written 16 days ago by • 0
0
votes
1
reply
111
views
ONT long-read RNA sequencing - data analysis options
DESeq2 LongRead DEXSeq IsoformSwitchAnalyzeR RNASeq
updated 16 days ago by 41k • written 16 days ago by • 0
1
vote
2
replies
703
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
17 days ago • updated 8 days ago ussarizona ▴ 10
0
votes
1
reply
130
views
covariate with negative and positive values
DESeq2
updated 16 days ago by 41k • written 17 days ago by • 0
0
votes
1
reply
145
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
updated 16 days ago by ★ 4.1k • written 17 days ago by • 0
0
votes
4
replies
235
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind DESeq2 ATACSeq
17 days ago • updated 14 days ago lonn • 0
0
votes
3
replies
203
views
Interaction Terms in DESeq2 - Shrinkage
DESeq2
updated 18 days ago by 41k • written 19 days ago by ▴ 20
1
vote
2
replies
239
views
How to retrieve detailed regression results from deseq2
DESeq2
23 days ago • updated 14 days ago Weiqian ▴ 10
0
votes
4
replies
261
views
DEG with low counts
DESeq2
updated 15 days ago by 41k • written 19 days ago by • 0
0
votes
1
reply
130
views
Deseq2 Rlog transformation
DESeq2
updated 19 days ago by ★ 4.1k • written 20 days ago by • 0
1
vote
2
replies
191
views
Design Matrix within group comparison more than two groups
DESeq2
22 days ago • updated 21 days ago hemantcnaik • 0
0
votes
1
reply
132
views
Bioinformatics student
Des DESeq2
updated 22 days ago by • 0 • written 22 days ago by • 0
0
votes
1
reply
174
views
DESeq2 gives an error and R crashes
DESeq2
23 days ago • updated 22 days ago Shefali • 0
0
votes
2
replies
218
views
Interaction term vs grouping variable design considerations
DESeq2 DifferentialExpression
updated 24 days ago by 41k • written 29 days ago by ▴ 30
0
votes
1
reply
160
views
IgG control in RIP-seq with Deseq2
DESeq2 RIP-seq
updated 24 days ago by 41k • written 25 days ago by • 0
0
votes
2
replies
241
views
Regressing out batch effects
DESeq2
updated 24 days ago by 41k • written 4 weeks ago by • 0
0
votes
2
replies
166
views
What to the log 2 fold changes mean using LRT on time course in DESeq2
DESeq2 timecourse DifferentialExpression
updated 29 days ago by 65k • written 29 days ago by • 0
1
vote
4
replies
474
views
DEseq2 coefficient
DESeq2 coefficient
10 weeks ago • updated 3 days ago JKim • 0
0
votes
7
replies
415
views
DESeq filtering specific to contrasts
DESeq2
updated 15 days ago by 41k • written 11 weeks ago by • 0
0
votes
4
replies
595
views
How to correct for known and surrogate variables in DESeq2?
DESeq2 BatchEffect sva limma
updated 18 days ago by 41k • written 6 months ago by • 0
2
votes
5
replies
1.1k
views
DESeq2 remove batch effect
BatchEffect RNASeqData WGCNA DESeq2
updated 17 days ago by • 0 • written 10 months ago by • 0
6
votes
19
replies
2.5k
views
DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
deseq2
updated 4 days ago by • 0 • written 4.6 years ago by ▴ 10
2
votes
5
replies
744
views
DESeq2 setting significant p-value
DESeq2
updated 15 days ago by 41k • written 5.0 years ago by ▴ 10
9
votes
12
replies
7.8k
views
Multi factor design edgeR/Deseq2
deseq2 edgeR rna-seq
updated 16 days ago by • 0 • written 8.8 years ago by ▴ 30
46 results • Page 1 of 1
Recent ...
Replies
Answer: Once again a "Model matrix not full rank" by swbarnes2 ★ 1.3k
Replicate numbers, like the 1 in control_1 are fine in sample names, but never add them to anything else in colData. From the computer's p…
Answer: limma Intercept vs No-intercept models completely changing DMR results? by Gordon Smyth 50k
I'll add a little bit of general advice to James' answer. You seem to have the misunderstanding that you can change the design matrix but …
Answer: package goseq seems to be not available on the latest version of R by Gordon Smyth 50k
It is true that goseq isn't available for Bioc 3.19. That is because goseq depends on txbmaker, which is itself not yet available for Bioc…
Answer: limma Intercept vs No-intercept models completely changing DMR results? by James W. MacDonald 65k
This part: ``` design_intercept <- model.matrix(~Alt+Sex+Age+PC1, data=targets_Sherpa) colnames(design_intercept) <- c("AltSHP_LA","Al…
Comment: CombineArrays for EPIC and EPIC V2 by Kim • 0
Thank you Tim, this is a great help in getting me started!
Votes
Answer: limma Intercept vs No-intercept models completely changing DMR results?
Answer: CombineArrays for EPIC and EPIC V2
Answer: Too many significant genes when integrating gtex and tcga
Comment: Too many significant genes when integrating gtex and tcga
A: Print Differentially Expressed Exons From Dexseq Results
Awards • All
Popular Question to Gordon Smyth 50k
Popular Question to Zainab • 0
Popular Question to Theo ▴ 10
Popular Question to gene_bioconductor ▴ 10
Popular Question to rohitsatyam102 ▴ 20
Locations • All
Ireland, 13 minutes ago
Germany, 34 minutes ago
France, 40 minutes ago
The city by the bay, 41 minutes ago
United States, 53 minutes ago
Traffic: 563 users visited in the last hour
Content Search
Users
Tags
Badges
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6