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DEseq2
•
reset
0
votes
2
replies
1.3k
views
DESeq2 LFC changes sign after adjusting for surrogates
sva
DESeq2
3.3 years ago
Marie
• 0
0
votes
5
replies
3.5k
views
Issues installing DESeq2 package with R version 4.2.1
DESeq2
3.3 years ago
lucabartolinimd
• 0
2
votes
2
replies
2.3k
views
How to get started with DeseqDataSet import using CSV? Is it necessary to have ColData command?
Metagenomics
DESeq2
updated 3.3 years ago by
ATpoint
★ 5.0k • written 3.3 years ago by
Muhammad
• 0
4
votes
5
replies
3.5k
views
Which is the best type of data for correlation or survival analysis
TPM
RNASeq
RSEM
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
Yang Shi
▴ 10
1
vote
1
reply
830
views
Handling heterogeneous data in DESeq2: multiple covariates to control against
RNASeq
DESeq2
updated 3.3 years ago by
ATpoint
★ 5.0k • written 3.3 years ago by
seeta_rajpara
• 0
2
votes
5
replies
1.6k
views
Detection of reference genes by Deseq2
lessAbs
reference_genes
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
sj
▴ 10
1
vote
5
replies
3.2k
views
DESeq2 outlier options and LFC Shrinkage
DESeq2
RNASeq
RnaSeqSampleSize
apeglm
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
Riley
• 0
1
vote
3
replies
1.6k
views
Swish: contrasts and interactions
fishpond
DESeq2
updated 2.6 years ago by
Michael Love
43k • written 3.3 years ago by
alexyz
• 0
2
votes
2
replies
1.1k
views
Contradictory results in smallRNA differential expression analysis using DESeq2
SmallRNA
DESeq2
3.3 years ago
jucosie
• 0
1
vote
1
reply
1.4k
views
Dispersion estimates with DESeq2
dispersionestimates
dispersion
DESeq2
estimates
deseq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
Julia
▴ 10
0
votes
6
replies
2.3k
views
Is DESeq2 generating false postives when changing different pre-filtering thresholds?
RNASeq
DEG
DESeq2
3.3 years ago
Weiqian
▴ 10
0
votes
1
reply
1.1k
views
DESeq2 - Batch effect correction in 2 conditions + 2 genotypes + interaction term design
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.4 years ago by
barbara.mariotti
• 0
1
vote
1
reply
1.3k
views
CQN to DESeq2
cqn
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
bioinfo
• 0
1
vote
5
replies
1.5k
views
what should be our references when 2 samples which I compare are treated and there is not any control?
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
Maryam.sed1400
• 0
0
votes
1
reply
1.1k
views
I have a query regarding differential gene expression using limma-voom.
RNASeqData
DESeq2
updated 3.3 years ago by
Gordon Smyth
53k • written 3.3 years ago by
btadfaramin
• 0
2
votes
1
reply
1.1k
views
Subsetting a DESeq object - gene-wise dispersions
DESeq2
updated 3.3 years ago by
ATpoint
★ 5.0k • written 3.3 years ago by
andrebolerbarros
▴ 20
0
votes
1
reply
997
views
DESeq2 Interaction Term Error Occurred
interaction
DESeq2
updated 3.3 years ago by
swbarnes2
★ 1.4k • written 3.3 years ago by
Tait
• 0
2
votes
7
replies
25k
views
Install DESeq2
DESeq2
3.3 years ago
Chris
▴ 20
0
votes
3
replies
1.8k
views
DESeq2 - transcript length correction necessary when quantifying reads from ends of transcripts?
Transcriptomics
DESeq2
3.3 years ago
marius.wenzel
• 0
1
vote
2
replies
2.2k
views
DESeq2 aggregated single cell data
pseudobulk
SingleCell
DESeq2
3.3 years ago
fluentin44
• 0
0
votes
1
reply
1.3k
views
Help with featureCounts
DESeq2
updated 3.3 years ago by
ATpoint
★ 5.0k • written 3.3 years ago by
lastarr
• 0
3
votes
2
replies
1.4k
views
DESeq2 is renaming gene names and lack of downregulated genes
DESeq
DESeq2
3.3 years ago
manwar
• 0
0
votes
8
replies
4.5k
views
DESeq2 different log2FC and p-value filtering parameters results comparison
log2
DESeq2
3.4 years ago • updated 3.3 years ago
User000
• 0
2
votes
4
replies
2.1k
views
pre-ranked GSEA or not?
log2FC
DESeq2
GSEA
voom
limma
updated 3.3 years ago by
Gordon Smyth
53k • written 3.4 years ago by
Yang Shi
▴ 10
1
vote
5
replies
1.4k
views
DeSeq2 Matched Analysis
RNASeq
DESeq2
3.4 years ago • updated 3.3 years ago
Andrea
• 0
0
votes
2
replies
2.8k
views
[DESeq2] setting LFC threshold
DESeq2
3.4 years ago
Shail
• 0
0
votes
1
reply
1.9k
views
lfcShrink types for Volcano plots
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
alanh
• 0
3
votes
4
replies
1.5k
views
Can I use limma::avereps to remove dupicated genes before conduct DEG based on RSEM Expected_count data?
limma
RNASeq
Expected_count
DESeq2
3.4 years ago
Yang Shi
▴ 10
0
votes
1
reply
800
views
DESeq2: correct way to extract comparison of comparisons
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
adrtsc
• 0
0
votes
1
reply
597
views
DESeq2 for CaptureSeq
CaptureSeq
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
alistair.poll
• 0
1
vote
6
replies
2.0k
views
Iterating over a column within colData@listData
DESeq2
iterating
SummarizedExperiment
3.4 years ago
BioInfoBeginnerrr
• 0
1
vote
3
replies
1.6k
views
LFC shrinkage setting negative LFC svalues to zero
DESeq2
DifferentialExpression
apeglm
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
81das_col
• 0
1
vote
7
replies
2.9k
views
DESeqDataSetFromMatrix error
BiocGenerics
DESeq2
DEGreport
RNASeqData
DEG
updated 3.4 years ago by
James W. MacDonald
68k • written 3.4 years ago by
Diya
• 0
0
votes
9
replies
2.7k
views
Questions about the false positives indicated by DESeq2 and QQ plot
pre-filterin
DESeq2
updated 2.1 years ago by
yujin
▴ 10 • written 3.4 years ago by
Weiqian
▴ 10
1
vote
3
replies
2.7k
views
ColData and ERROR: rows did not converge in beta, labelled in mcols(object)$betaConv. Use larger maxit argument with nbinomWaldTest
DESeq2
updated 12 months ago by
Michael Love
43k • written 3.4 years ago by
LorManj
• 0
0
votes
1
reply
894
views
DESeq2 LRT not returning genes
LRT
linearmodels
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
meeta.mistry
▴ 30
1
vote
2
replies
3.0k
views
DEseq2 anlysis - LfcThreshold and Lfcshrink options
lfcshrink
DESeq2
RNASeq
lfcthreshold
3.4 years ago
ClaudiaE
• 0
1
vote
12
replies
4.5k
views
How to use "p-value" instead of "p-adj value" in DESeq2
DESeq2
updated 2.1 years ago by
Assa Yeroslaviz
★ 1.5k • written 3.4 years ago by
Maryam.sed1400
• 0
1
vote
1
reply
662
views
Error message : DESeq2
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
Md. Faridul
• 0
1
vote
1
reply
935
views
result does not change after relevel
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
JaneDoe
• 0
0
votes
14
replies
6.2k
views
GenomeInfoDbData had non-zero exit status
DESeq2
RStudio
GenomeInfoDbData
hadnon-zeroexitstatus
updated 3.2 years ago by
wshao9
• 0 • written 3.4 years ago by
JoseR
• 0
2
votes
2
replies
920
views
DESeq2 question
DESeq2
3.4 years ago
Yu
• 0
0
votes
4
replies
1.5k
views
Multi-factorial Time-based design in DEseq2: defining full and reduced model
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
Alex
• 0
1
vote
1
reply
801
views
how do singleton samples contribute to dispersion estimate in DESeq2?
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
Victor
▴ 10
1
vote
4
replies
7.2k
views
Error message: Could not find function "DESeqDataSetFromMatrix"
DESeq2
3.4 years ago
Md. Faridul
• 0
0
votes
1
reply
797
views
Error
DESeq2
updated 3.4 years ago by
ATpoint
★ 5.0k • written 3.4 years ago by
Mahsa
• 0
2
votes
8
replies
5.0k
views
How to get DESeq2 results for a list of genes
DESeq2
3.4 years ago
Lucía
▴ 30
1
vote
1
reply
1.3k
views
setting sizeFactors from scran :: caculateSumFactors.
scran
DESeq2
updated 3.4 years ago by
ATpoint
★ 5.0k • written 3.4 years ago by
OE
• 0
0
votes
4
replies
1.9k
views
Alternative for wininet method in tximeta
DESeq2
tximport
tximportData
tximeta
3.4 years ago
uhlkatie
• 0
0
votes
3
replies
2.3k
views
How can i fix this problem in R
S4Vectors
DESeq2
updated 3.4 years ago by
ATpoint
★ 5.0k • written 3.4 years ago by
Habib
• 0
4,839 results • Page
20 of 97
Recent ...
Replies
Comment: upstream git branches of bioc-release
by
TrentonCollier99
• 0
Thank you very much for taking the time to answer the question. I really appreciate your insights and comments. https://bioc-release.r-univ…
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
by
Ahmed Salah
• 0
Thank you very much! will take that into consideration
Comment: Large logFC but somewhat high FDR
by
JKim
• 0
Thank you very much for taking the time to answer the question. I really appreciate your insights and comments.
Comment: upstream git branches of bioc-release
by
Jeroen
▴ 10
OK I have updated https://bioc-release.r-universe.dev accordingly. Let me know if you find version differences when mirroring binaries.
Comment: Large logFC but somewhat high FDR
by
Gordon Smyth
53k
I don't know what `tp1` is. The relevant histogram would be: ``` hist(fit2$p.value[,"KO_vs_WT"]) ```
Votes
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Large logFC but somewhat high FDR
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
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