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DeSeq2
•
reset
0
votes
2
replies
760
views
RNA-Seq p value range variation
DESeq2
pvalue
RNA-Seq
16 months ago
paul.meraner
• 0
0
votes
0
replies
673
views
News:
RNAseq data analysis with Bioconductor (online course)
DESeq2
SummarizedExperiment
16 months ago • updated 15 months ago
info
▴ 10
1
vote
6
replies
1.6k
views
heatmap with batch effect
DESeq2
BatchEffect
written 16 months ago by
tothuhien
• 0
0
votes
2
replies
820
views
How can I obtain DEGs that are resulted from using salmon in alignment-based mode or mapping-based mode?
tximport
DESeq2
updated 16 months ago by
Michael Love
43k • written 16 months ago by
oguzhankaraosmanoglu
• 0
1
vote
1
reply
1.3k
views
Deseq2 sfType
Microbiome
DESeq2
RNASeq
updated 16 months ago by
James W. MacDonald
68k • written 16 months ago by
Caroline
• 0
1
vote
1
reply
756
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 16 months ago by
ATpoint
★ 5.0k • written 16 months ago by
Pimpa
• 0
1
vote
1
reply
626
views
DEseq 2 LFC shrinkage with apeglm in single-cell pseudobulk: strange volcano plot results
DESeq2
updated 16 months ago by
ATpoint
★ 5.0k • written 16 months ago by
picasa1983
• 0
1
vote
2
replies
965
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 16 months ago by
Michael Love
43k • written 16 months ago by
Ben
▴ 10
0
votes
2
replies
1.1k
views
DESeq2 analysis on 2 plant lines infected with virus, with 2 time points (days post infection)
DESeq2
updated 16 months ago by
Michael Love
43k • written 16 months ago by
duplessisantzel
• 0
1
vote
2
replies
1.1k
views
DESeq2 design and contrasts with 2 drug-treated and one control group
DESeq2
Bioconductor
updated 16 months ago by
Michael Love
43k • written 16 months ago by
JT
▴ 10
1
vote
6
replies
2.1k
views
New to Deseq2 - DEG questions / MA plot
genomes
DESeq2
Transcriptomics
updated 15 months ago by
ATpoint
★ 5.0k • written 16 months ago by
Newbie
• 0
0
votes
1
reply
504
views
Many DEGs (Is a high percentage of DEGs acceptable in DESeq2)?
DESeq2
updated 16 months ago by
Michael Love
43k • written 16 months ago by
madivinakristidiscar
• 0
17
votes
9
replies
8.5k
views
How to use removeBatchEffect for removing effect of multiple confounding variables
limma
removebatcheffect
deseq2
updated 16 months ago by
James W. MacDonald
68k • written 9.7 years ago by
ompandey
▴ 10
0
votes
1
reply
685
views
Dealing with 0 miRNA counts in DESeq2 analysis and influence in adjusted p-value
microRNAome
DESeq2
updated 16 months ago by
Michael Love
43k • written 16 months ago by
eric
• 0
0
votes
2
replies
890
views
PCA analysis RNA-Seq QC looks strange?
DESeq2
updated 16 months ago by
swbarnes2
★ 1.4k • written 17 months ago by
Megan
• 0
0
votes
5
replies
2.4k
views
DESeq2 Multi-factor design not full rank
RNASeq
DESeq2
written 4.7 years ago by
jehimi
• 0
3
votes
6
replies
2.1k
views
[DESeq2] Normalization of External Test Dataset for Machine Learning (ML) Application
DESeq2
updated 17 months ago by
Michael Love
43k • written 17 months ago by
micolak0115
• 0
3
votes
6
replies
32k
views
Deseq: What does "BaseMean" mean?
deseq2
written 7.4 years ago by
183227851
• 0
0
votes
2
replies
1.8k
views
Understanding Contrasts and Interaction in DESeq2 for 2 genotypes, 2 treatments
RNASeq
DESeq2
updated 17 months ago by
swbarnes2
★ 1.4k • written 17 months ago by
Shwetal
• 0
4
votes
11
replies
1.9k
views
Size factor normalized counts for between sample comparisons - correct?
DESeq2
17 months ago
boczniak767
▴ 740
3
votes
4
replies
901
views
Gene filtering in DESeq2
DESeq2
updated 17 months ago by
ATpoint
★ 5.0k • written 17 months ago by
Claudio
• 0
0
votes
2
replies
889
views
Inquiry on Using DESeq2 for Analyzing Differential Translation Efficiency
RiboSeq
DESeq2
written 17 months ago by
noi.cohen
• 0
1
vote
2
replies
528
views
Deseq2 - differenc in results
DESeq2
written 17 months ago by
rajan
• 0
0
votes
7
replies
1.2k
views
DESeq design with and without intercept is giving different results
DESeq2
written 17 months ago by
ktz
• 0
1
vote
5
replies
1.7k
views
RNASeq normlization for samples with global shift in gene expression
qsmooth
DESeq2
limma
Normalization
updated 17 months ago by
Gordon Smyth
53k • written 17 months ago by
Xuan Yi
• 0
0
votes
3
replies
1.2k
views
More significant genes after lfcShrink in DEseq2?
DESeq2
written 17 months ago by
m.orlov
• 0
0
votes
3
replies
1.0k
views
Differential expression analysis in time course experiments with DESeq2
DESeq2
limma
DifferentialExpression
updated 17 months ago by
Gordon Smyth
53k • written 17 months ago by
Jiahao Tian
• 0
0
votes
2
replies
737
views
Analysis of transcription inhibition with DESeq2
SpikeIn
DESeq2
Normalization
written 17 months ago by
ADopico
• 0
6
votes
5
replies
4.8k
views
What is the importance of `--countReadPairs` in featureCounts on paired end data?
featureCounts
DESeq2
updated 13 months ago by
rohitsatyam102
▴ 20 • written 17 months ago by
Bioinformagician
• 0
3
votes
3
replies
1.9k
views
the count matrix have decimal values
DESeq2
DifferentialExpression
updated 17 months ago by
James W. MacDonald
68k • written 17 months ago by
Ibrahim
• 0
0
votes
2
replies
1.3k
views
R is Removing the Gene Names in My Count Data
DESeq2
updated 17 months ago by
BioinfGuru
▴ 70 • written 17 months ago by
thatolivebranch
• 0
1
vote
4
replies
1.7k
views
Recommendations for DESeq2 Independent Filtering with Pseudobulk Data
DESeq2
independentfiltering
glmGamPoi
18 months ago • updated 17 months ago
Bob
▴ 30
0
votes
1
reply
656
views
Using DESeq2 for Analyzing AMR Gene Variations in Water Samples Post-Treatment
DESeq2
NanoporeRNASeq
DNASeq
updated 17 months ago by
Michael Love
43k • written 17 months ago by
Dot
• 0
0
votes
3
replies
1.2k
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2
edgeR
DifferentialExpression
written 17 months ago by
dancingcow
• 0
3
votes
5
replies
2.7k
views
DESeq2: How to Compare the Condition Effects of Two Different Treatments?
DESeq2
RNAseq
DifferentialExpression
updated 17 months ago by
swbarnes2
★ 1.4k • written 18 months ago by
BioNovice247
• 0
2
votes
1
reply
1.9k
views
DESeq2 is it OK to scale transformed data in order to plot heatmaps?
DESeq2
heatmaps
updated 17 months ago by
ATpoint
★ 5.0k • written 17 months ago by
jeba95
• 0
2
votes
2
replies
951
views
Why is the inclusion of RUVs not reflected in the EDA plots produced from normalised counts extracted from deseq2?
RUVSeq
DESeq2
dese
17 months ago
BioinfGuru
▴ 70
1
vote
2
replies
840
views
Combining DESeq2 and Within-Group Variability to Identify Stochastic Genes
DESeq2
18 months ago • updated 17 months ago
DL
• 0
0
votes
3
replies
987
views
RNA-seq with DESeq2 shrinkage estimation
Regression
DESeq2
rnaseqGene
updated 18 months ago by
James W. MacDonald
68k • written 18 months ago by
David W. H.
• 0
6
votes
15
replies
2.7k
views
Deseq2 design formula and analysis with internal controls
DESeq2
updated 17 months ago by
swbarnes2
★ 1.4k • written 18 months ago by
kubracelikbas4
• 0
1
vote
2
replies
1.4k
views
How to use specific size factors and average transcript lengths from tximport in DESeq2?
DESeq2
tximport
18 months ago
grothmn
• 0
0
votes
1
reply
1.4k
views
HELP - DESeq2 difference between LRT and Wald Test in RNA-seq/ATAC-seq
DESeq2
LRT
Wald
updated 18 months ago by
James W. MacDonald
68k • written 18 months ago by
Eric
• 0
4
votes
7
replies
2.6k
views
What does evidence of batch effect in bulk rnaseq look like?
surrogatevariables
DESeq2
BatchEffect
bulkrna-seq
sva-seq
updated 18 months ago by
jessica.anderson
▴ 10 • written 19 months ago by
BioinfGuru
▴ 70
0
votes
1
reply
942
views
DESeq2 - Outliers influencing sig DEGs, filter counts per group samples, not by all samples
filtering
DESeq2
outliers
updated 18 months ago by
Michael Love
43k • written 18 months ago by
smac_head
• 0
0
votes
1
reply
1.1k
views
DESeq2 Likelihood ratio test + contrast option + Volcano plot
DESeq2
volcanoplot
LRT
updated 18 months ago by
Michael Love
43k • written 18 months ago by
Eric
• 0
2
votes
3
replies
1.1k
views
Comparing one group against multiple groups in DESeq2
DESeq2
written 2.0 years ago by
tamivico
• 0
2
votes
3
replies
3.3k
views
Plot PCA with ellipses using ggplot
DESeq2
ggplot
PCA
written 18 months ago by
robledoj13
• 0
3
votes
6
replies
1.4k
views
A question occur when using DESeq2 on a complex design
DESeq2
rnaseqGene
RNASeq
written 18 months ago by
MedocRafael
• 0
4
votes
3
replies
1.7k
views
DESeq fitNbinomGLMs Warning Issue: In fitNbinomGLMs(objectNZ[fitidx, , drop = FALSE], alpha_hat = alpha_hat[fitidx], : 4189rows had non-positive e…
DESeq2
RNAseq
written 18 months ago by
mila
▴ 10
0
votes
3
replies
1.1k
views
Error when trying to do different pairwise comparisons and lfcshrink than what's listed on resultsNames()
RNASeqData
DESeq2
RNASeq
DifferentialExpression
updated 18 months ago by
ATpoint
★ 5.0k • written 18 months ago by
Mark
• 0
4,848 results • Page
4 of 97
Recent ...
Replies
Comment: msmsTests, inconsistent sign between lFC.Av and LogFC
by
Ben
• 0
Continued here due to posting difficulties: Extract from the results table: ``` Inhibitor Vehicle lFC.Av LogFC ... F1 0.0 27…
Answer: DESeq2 for 16S rRNA Analysis
by
James W. MacDonald
68k
The Wald test is only meant for comparison of two groups at a time, so it depends on what you mean by 'analyze across multiple groups at a …
Comment: MetaDE.pvalue: Error in xj[i]: invalid subscript type 'list'
by
varying.mastodon.isyn
• 0
Oh, that error! I remember wrestling with that one for hours when I was first learning Python. Turns out I was trying to use a list itself …
Comment: Confirm that OpenStats will not be archived (pryr dependency)
by
Marina
• 0
Thank you!
Comment: AnnotationForge makeOrgPackage rsqlite_send_query error
by
ansley.r.burtch
• 0
Hi! I am sure you are well into your career now and haven't worked on this for a while, but I wanted to let you know this is still helping …
Votes
A: DESeq2 - VST eliminates zero values
Comment: Normalise to a housekeeping gene in DESEq2
Comment: limpa analysis advice
C: AnnotationForge makeOrgPackage rsqlite_send_query error
Answer: Limpa Pipeline Order
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