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Microbiome
•
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1
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1
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137
views
Software Compatibility Issue Between phyloseq and lme4 Packages
phyloseq
microbiome
Software
updated 9 days ago by
ATpoint
★ 3.4k • written 10 days ago by
HULFRED
• 0
0
votes
0
replies
97
views
Try to understand better how to set out an effective sample size within the MetagenomSeq package in microbiome ?
Metagenomics
metagenomeSeq
microbiome
phyloseq
microbiomeDataSets
5 weeks ago
Mohamed
▴ 20
0
votes
1
reply
240
views
Query regarding phyloseq object construct with QIIME output
phyloseq
microbiome
Metagenomics
written 3 months ago by
abhisek001
• 0
0
votes
2
replies
634
views
stat_compare_means function - Error 'must have same length'
microbiomeDataSets
Microbiome
7 months ago
Elif
• 0
1
vote
0
replies
272
views
News:
Virtual talk “Model Misspecification in Microbiome Studies” by Amy D Willis
MicrobiomeData
Microbiome
9 months ago
Levi Waldron
1.1k
1
vote
3
replies
5.5k
views
DESeq2 - Error: full model matrix is less than full rank
microbiome
DESeq2
updated 12 months ago by
adR
▴ 30 • written 2.9 years ago by
Daniel
• 0
0
votes
1
reply
405
views
How to add rowLinks, rowTree in SummarizedExperiment manually.
TreeSummarizedExperiment
phylogenetic
microbiome
SummarizedExperiment
updated 12 months ago by
James W. MacDonald
63k • written 12 months ago by
Muhammad
• 0
0
votes
3
replies
1.0k
views
I cannot install "microbiome"
GenomeInfoDbData
microbiome
phyloseq
updated 13 months ago by
Marcel Ramos
680 • written 13 months ago by
kikiki
• 0
1
vote
1
reply
1.0k
views
Deseq2 for 16s Data
Microbiome
16s
Deseq2
16srRNA
updated 17 months ago by
Michael Love
40k • written 17 months ago by
Katelyn
• 0
0
votes
1
reply
565
views
DESeq2 differences in Wald and LRT in multi-factorial design
microbiome
LRT
DESeq2
updated 20 months ago by
Michael Love
40k • written 20 months ago by
Mir
• 0
0
votes
1
reply
412
views
pairwise within sample
DESeq2
microbiome
updated 2.1 years ago by
Michael Love
40k • written 2.1 years ago by
laubitz
• 0
0
votes
1
reply
644
views
Editing Plots Generated Using MicrobiomeAnalyst
MicrobiomeData
microbiome
offtopic
updated 2.2 years ago by
James W. MacDonald
63k • written 2.2 years ago by
gwagz8
• 0
0
votes
1
reply
645
views
Adding insect abundance data to SparCC Analysis
sparcc
Microbiome
microbiomeExplorer
MicrobiomeData
updated 2.2 years ago by
Kevin Blighe
3.8k • written 2.2 years ago by
GlycineMax
• 0
0
votes
0
replies
691
views
Pattern Search in MicrobiomeAnalyst
metagenomeSeq
Metagenomics
microbiome
microbiomeExplorer
2.3 years ago
GlycineMax
• 0
0
votes
0
replies
803
views
News:
Early career researchers: nominate to present your work at BVCN virtual conference on microbiome research
microbiome
news
Conference
2.6 years ago
Matthew Ritchie
▴ 1000
0
votes
2
replies
897
views
Question: export edgeR normalised OTU table
microbiome
edgeR
2.7 years ago
Pozdrawiam
• 0
0
votes
2
replies
1.3k
views
Differential Abundance Analysis wih DESeq2 and metabolites as continuous predictor variables
deseq2
microbiome
R
updated 3.0 years ago by
Michael Love
40k • written 3.0 years ago by
greenm11
• 0
0
votes
1
reply
1.0k
views
MetagenomeSeq: analysing paired samples
metagenomeseq
paired samples
microbiome
updated 3.5 years ago by
jananee15
• 0 • written 6.8 years ago by
marypadilha
• 0
0
votes
2
replies
615
views
DESeq2: seeking advice regarding before/after mutliple treatements + extra factor
deseq2
16s
microbiome
updated 3.7 years ago by
Michael Love
40k • written 3.7 years ago by
mstagliamonte
▴ 10
0
votes
0
replies
767
views
Job:
Bioinformatician Positions at the University of Chicago BSD Bioinformatics Core
RNA-seq
DNA-seq
ATAC-seq
proteomics
microbiome
Job
3.7 years ago
lhuang7
▴ 50
1
vote
0
replies
672
views
Job:
Bioinformatician: NEW YORK CITY - Institute for Data Science and Genomic Technology, Mount Sinai School of Medicine
job
bioinformatician
systems biology
microbiome
genomics
Job
4.0 years ago
supindab
▴ 10
0
votes
2
replies
1.0k
views
DESeq2 error on analyzing microbiome data
deseq2
microbiome
sequencing
4.2 years ago
zhigang.li
• 0
0
votes
2
replies
1.2k
views
DESeq2 Interaction Term Interpretation for Microbiome Data
deseq2
microbiome
updated 4.3 years ago by
swbarnes2
★ 1.3k • written 4.3 years ago by
ehickman
• 0
0
votes
0
replies
728
views
Aldex2 feature request - controlling for additional variables
ALDEx2
ALDEx
microbiome
16s
4.8 years ago
mstagliamonte
▴ 10
0
votes
1
reply
907
views
metagenomeSeq error question: error on creation of newMRexperiment
metagenomeseq error
metagenomeseq
microbiome
4.8 years ago
slizo001
• 0
2
votes
1
reply
867
views
Rsubread for microbiome data analysis: genome >4GB
alignment
rsubread
microbiome
updated 4.9 years ago by
Wei Shi
★ 3.5k • written 4.9 years ago by
W. Evan Johnson
▴ 850
0
votes
1
reply
823
views
Question about multi-factor Deseq analysis
DESeq
deseq2
microbiome
gut microbiome
updated 4.9 years ago by
Michael Love
40k • written 4.9 years ago by
FaisalH
• 0
0
votes
1
reply
1.4k
views
DeSeq2 for comparison of experimental factors on microbial composition
deseq2
microbiome
fungi
fungal microbiome
updated 5.4 years ago by
Michael Love
40k • written 5.4 years ago by
david.gramaje
• 0
0
votes
0
replies
1.0k
views
Job:
EXTENDED: Bioinformatics Specialist at the University of Illinois
microbiome
epigenomics
bioinformatics
analyst
NGS
Job
5.5 years ago
Jenny Drnevich
★ 2.0k
0
votes
0
replies
1.0k
views
Job:
Bioinformatics Specialist at the University of Illinois
job
bioinformatics
microbiome
metagenomics
epigenomics
Job
5.6 years ago
Jenny Drnevich
★ 2.0k
1
vote
1
reply
1.2k
views
Getting OTUID rather than Taxonomy from Results function in DESeq2
deseq2
R
microbiome
phyloseq
qiime
updated 5.8 years ago by
Michael Love
40k • written 5.8 years ago by
ssingh59
• 0
1
vote
1
reply
1.4k
views
Should I normalize communities separately with metagenomeseq?
metagenomeseq
microbiome
gut microbiome
metagenomics
shotgun metagenomics
updated 5.9 years ago by
Joseph Nathaniel Paulson
▴ 280 • written 5.9 years ago by
lilianeconteville
▴ 10
3
votes
2
replies
2.8k
views
DESeq2 iterative size factor normalization did not converge
deseq2
microbiome
phyloseq
updated 6.1 years ago by
Michael Love
40k • written 6.1 years ago by
couchc
• 0
0
votes
6
replies
1.4k
views
Why DESeq2 gives large logfold for group with all zero count?
microbiome
16s
deseq2
differential gene expression
6.3 years ago
lanhuong
▴ 20
0
votes
3
replies
1.4k
views
Where to find open access microbiome data for case-control comparative study?
microbiome
data analysis
data request
updated 6.5 years ago by
Wolfgang Huber
13k • written 6.5 years ago by
stats134711
• 0
1
vote
1
reply
3.1k
views
Normalization/Transformation Before Microbiome Alpha Diversity Analysis
microbiome
normalization
variancestabilizingtransformation
phyloseq
6.5 years ago
stats134711
• 0
0
votes
2
replies
1.3k
views
metagenomeSeq: cumNormStat normalization default
metagenomeseq
microbiome
6.8 years ago
Brian Smith
▴ 120
6
votes
1
reply
2.7k
views
Using R package 'limma' on pre-normalized counts and the effect of non-equivalent reads/sample on limma linear model
limma
rna-seq
multi-mapped reads
normalization
microbiome
updated 7.0 years ago by
Steve Lianoglou
13k • written 7.0 years ago by
tealfurn
▴ 20
0
votes
5
replies
3.2k
views
DESeq2 16s microbiome size factors
deseq2
metagenomeseq
rnaseq
16s
microbiome
updated 7.1 years ago by
Joseph Nathaniel Paulson
▴ 280 • written 7.1 years ago by
jbatsx
• 0
0
votes
8
replies
2.9k
views
Variance stabilizing transformation seems not working on NGS (bacterial 16srRNA based) data
Microbiome
DESeq2
Microbiome
DESeq2
updated 7.1 years ago by
Wolfgang Huber
13k • written 9.8 years ago by
Michael Love
40k
0
votes
0
replies
1.1k
views
Job:
2 year bioinformatics postdoc in the microbiome and prostate cancer risk @ UEA, Norwich, UK
Cancer
microbiome
postdoc
UK
prostate cancer
Job
7.1 years ago
Daniel Brewer
▴ 100
4
votes
3
replies
1.8k
views
Analyzing microbiome data (many zero-counts) using DESeq2
DESeq2
microbiome
zero-inflated
updated 7.2 years ago by
Michael Love
40k • written 7.2 years ago by
pjgalli2
• 0
0
votes
5
replies
1.3k
views
deseq2 analysis, accounting for pen effect
deseq2
microbiome
updated 7.9 years ago by
Steve Lianoglou
13k • written 7.9 years ago by
bjarke
▴ 10
2
votes
1
reply
1.6k
views
Drastic DE result discrepancies between versions of DESeq2
deseq2
differential expression
microbiome
updated 8.1 years ago by
Michael Love
40k • written 8.1 years ago by
drei
• 0
1
vote
2
replies
1.7k
views
Normalization factors in metagenomeSeq
microbiome
8.5 years ago
jovel_juan
▴ 10
2
votes
4
replies
2.0k
views
normFactors when aggregating in metagenomeSeq
metagenomeSeq
microbiome
updated 9 months ago by
Muhammad
• 0 • written 8.6 years ago by
noelle.noyes
▴ 30
1
vote
1
reply
2.8k
views
Low quantile estimate using CSS normalization in metagenomeSeq
metagenomeSeq
microbiome
updated 8.7 years ago by
Joseph Nathaniel Paulson
▴ 280 • written 8.7 years ago by
noelle.noyes
▴ 30
1
vote
1
reply
1.4k
views
Problem reproducing metagenomeSeq tutorial example
metagenome
microbiome
metagenomeseq
updated 8.7 years ago by
Joseph Nathaniel Paulson
▴ 280 • written 8.7 years ago by
jovel_juan
▴ 10
2
votes
2
replies
1.1k
views
no timeInterval output using fitTimeSeries
metagenomeSeq
fitTimeSeries
microbiome
8.7 years ago
noelle.noyes
▴ 30
2
votes
2
replies
1.4k
views
error using fitTimeSeries from metagenomeSeq
metagenomeSeq
microbiome
8.7 years ago
noelle.noyes
▴ 30
50 results • Page
1 of 1
Recent ...
Replies
Answer: Batch effect adjust in model matrix
by
ATpoint
★ 3.4k
Each sample is a different day, that is called "nested", so there is nothing you can do here. Next time do a better experimental design, he…
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Michael Love
40k
Oh, I see. If you can't use counts + offset then I prefer lengthScaledTPM to DESeq2, i.e. `txi$counts` to `DESeqDataSetFromMatrix()`. So …
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Christian
• 0
I´m sorry causing confusion here, but the gene matrices I refer to are actually obtained via https://nf-co.re/rnaseq. The problem with the…
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Michael Love
40k
For the reasons outlined in the Soneson paper, I'd prefer this pipeline: https://nf-co.re/rnaseq You cannot use these scaled TPM approach…
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
by
Christian
• 0
I get your point as we already starting with TPM. This was more to clarify that I understand the principle correctly. However, further I´m…
Votes
batch effect : comBat or blocking in limma ?
Batch correction and repeated measures
Comment: Does countsFromAbundance="lengthScaledTPM" produce un-normalized counts?
Comment: Phenotype effect - building a contrast matrix - grouping vs interaction
Answer: Batch correction and repeated measures
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