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EDGER
•
reset
0
votes
0
replies
14
views
How to model DE for RNA-seq in concordant monozygotic twins?
RNASeq
variancePartition
RUVSeq
limma
edgeR
13 hours ago
ilaria.massaiu
• 0
0
votes
0
replies
27
views
Streamlining the computing time for MiloDE p-value correction in large dataset?
SingleCellExperiment
edgeR
miloR
Bioconductor
2 days ago
Joseph
• 0
0
votes
2
replies
128
views
replace cat with message in glmTreat when lfc = 0
edgeR
glmTreat
updated 13 days ago by
Gordon Smyth
53k • written 17 days ago by
oliverd
• 0
0
votes
4
replies
275
views
voomLmFit fitted log-CPM values
limma
edgeR
updated 26 days ago by
Gordon Smyth
53k • written 27 days ago by
Lina
▴ 10
2
votes
9
replies
743
views
Transcript expression analysis in edgeR from gtf file obtained from merging long read rna-seq and standard gencode gtf
RNASeq
edgeR
LongRead
Transcript
7 weeks ago
mohammedtoufiq91
▴ 10
3
votes
2
replies
796
views
Transcript expression analysis using EdgeR
edgeR
Transcript
RNASeq
10 weeks ago
mohammedtoufiq91
▴ 10
0
votes
1
reply
872
views
Using offset in edgeR
edgeR
updated 10 weeks ago by
Gordon Smyth
53k • written 11 weeks ago by
mmadrzyk
• 0
4
votes
8
replies
1.8k
views
Should Xenium pseudobulk experiments be analysed like microarrays (limma) or like RNASeq (edgeR,Deseq2, or limma-voom)
Deseq2
edgeR
limma
xenium
Voom
11 weeks ago
richardallenfriedmanbrooklyn
▴ 30
7
votes
9
replies
5.4k
views
competitive enrichment test yields too few significant results, self-contained too many
limma
DESeq2
fry
edgeR
11 weeks ago
bbao
▴ 10
5
votes
11
replies
3.0k
views
parameters of filterByExpr
edgeR
DESeq2
filterByExpr
written 5 months ago by
caroline.zanchi
▴ 10
2
votes
4
replies
2.5k
views
Altered (negative or positive) logFC values for same gene from two intitial count matrices, one being a subset of the other
Transcriptomics
edgeR
DEG
4 months ago
Maziya
• 0
2
votes
3
replies
1.3k
views
Differential gene expression analysis on haplotype-resolved diploid assembly
edgeR
DESeq2
haplotype
limma
4 months ago
Paulito
• 0
0
votes
2
replies
815
views
edgeR in DMR mode - replicates give batch effect - how to build design matrix with condition and replicate
edgeR
updated 4 months ago by
Paul Harrison
▴ 110 • written 4 months ago by
Francesco
• 0
0
votes
3
replies
914
views
Model performance for spline modelling of RNA-seq timecourses in edgeR
edgeR
limma
written 5 months ago by
skiaphrene
▴ 10
10
votes
13
replies
2.3k
views
edgeR::cpm() results in negative values from raw counts of 0
edgeR
updated 5 months ago by
Gordon Smyth
53k • written 5 months ago by
Kinnary
▴ 20
2
votes
2
replies
1.4k
views
Improving performance of edgeR and limma while adjusting for multiple confounders
edgeR
limma
updated 6 months ago by
ATpoint
★ 4.9k • written 6 months ago by
robinpaul85
• 0
5
votes
3
replies
1.1k
views
Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with multiple cell types
edgeR
limma
voomLmFit
updated 6 months ago by
Gordon Smyth
53k • written 6 months ago by
Devin
▴ 10
2
votes
2
replies
1.5k
views
Limma - Complex 2-way multi-level contrast with paired samples
edgeR
contrastmatrix
limma
6 months ago
Elizabeth
• 0
0
votes
2
replies
680
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 6 months ago by
Michael Love
43k • written 6 months ago by
rohitsatyam102
▴ 20
0
votes
1
reply
404
views
Is it possible to model DNA methylation as a dose response in edgeR?
edgeR
updated 7 months ago by
Gordon Smyth
53k • written 7 months ago by
Isaiah
• 0
0
votes
4
replies
1.4k
views
DiffBind edgeR normalization --- why are the normalization factors all equal to 1?
edgeR
DiffBind
updated 7 months ago by
Harry
• 0 • written 7 months ago by
tifaGis
• 0
2
votes
4
replies
847
views
EdgeR Biological CVs
edgeR
written 8 months ago by
James
• 0
3
votes
5
replies
1.8k
views
Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
limma
edgeR
biomaRt
updated 8 months ago by
James W. MacDonald
68k • written 8 months ago by
Jonathan
▴ 10
0
votes
2
replies
611
views
Mean variance curve troubleshoot filtering
limmavoom
filtering
edgeR
RNASeqData
meanvariancetrend
9 months ago • updated 8 months ago
gingergeiger22
• 0
0
votes
1
reply
566
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 9 months ago by
Michael Love
43k • written 9 months ago by
Jimmy Mak
• 0
1
vote
8
replies
1.5k
views
Incorporating Salmon offset matrix into Wilcoxon DEG analysis
edgeR
9 months ago • updated 8 months ago
Andre
• 0
1
vote
9
replies
1.4k
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 9 months ago by
anna.cot.anna.cot
▴ 30
6
votes
10
replies
3.3k
views
What type of counts data to import for performing Isoform analysis in edgeR
limma
isoform
edgeR
salmon
R
updated 9 months ago by
ATpoint
★ 4.9k • written 10 months ago by
mohammedtoufiq91
▴ 10
7
votes
6
replies
1.3k
views
ANOVA like approach of edgeR
edgeR
updated 10 months ago by
Gordon Smyth
53k • written 10 months ago by
Talip
▴ 10
3
votes
3
replies
865
views
Is it recommended to analyze different tissues together when using edgeR
DifferentialExpression
edgeR
updated 10 months ago by
Gordon Smyth
53k • written 10 months ago by
Lars Roed
• 0
2
votes
4
replies
2.4k
views
Differential Expression Analysis with unbalanced batches without replication between conditions
edgeR
BatchEffect
updated 10 months ago by
Gordon Smyth
53k • written 10 months ago by
Thili
• 0
1
vote
8
replies
2.4k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 11 months ago by
Adam Marstrand
• 0
0
votes
1
reply
845
views
single cell RNA seq pseudobulk batch correction
edgeR
updated 11 months ago by
James W. MacDonald
68k • written 11 months ago by
mairahtkhan
• 0
3
votes
3
replies
1.5k
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 11 months ago by
Satoshi
• 0
0
votes
4
replies
993
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs
edgeR
RNASeqData
11 months ago
Philippine
• 0
4
votes
6
replies
1.7k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
11 months ago
altintas.ali
• 0
1
vote
2
replies
663
views
edgeR 4.2.2 not compiled for Windows
edger
edgeR
11 months ago
Hongjian
• 0
3
votes
3
replies
684
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
11 months ago
Guido Hooiveld
★ 4.1k
4
votes
1
reply
615
views
edgeR 4.2.1 compression bug: a minimally reproducible snippet
edger
edgeR
updated 11 months ago by
Gordon Smyth
53k • written 11 months ago by
Hongjian
• 0
1
vote
1
reply
532
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 12 months ago by
ATpoint
★ 4.9k • written 12 months ago by
Pimpa
• 0
1
vote
2
replies
824
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 12 months ago by
Michael Love
43k • written 12 months ago by
Ben
▴ 10
0
votes
2
replies
740
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 12 months ago by
Gordon Smyth
53k • written 12 months ago by
blmurphy
• 0
1
vote
2
replies
654
views
Inconsistent result in edgeR
edgeR
12 months ago
hiroko.ohmiya
• 0
1
vote
1
reply
806
views
Help with design matrix for RNA-seq data using edgeR
edgeR
RNASeqData
updated 13 months ago by
Gordon Smyth
53k • written 13 months ago by
erik.burchard
• 0
2
votes
9
replies
1.4k
views
Seeking Advice on Using edgeR and variancePartition for RNA-seq Data from Multiple Tissues and Patients
edgeR
variancePartition
updated 13 months ago by
Gordon Smyth
53k • written 13 months ago by
Seymoo
• 0
0
votes
3
replies
964
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2
edgeR
DifferentialExpression
written 13 months ago by
dancingcow
• 0
2
votes
1
reply
2.0k
views
Expected memory usage for analyzing large single-cell seq datasets (> 450,000 cells)?
BigData
Clustering
memory
SingleCellExperiment
edgeR
updated 13 months ago by
Aaron Lun
★ 29k • written 14 months ago by
Joseph
• 0
0
votes
1
reply
718
views
edgeR - design a complex matrix
design
model.matrix
edgeR
complexdesign
updated 13 months ago by
Gordon Smyth
53k • written 14 months ago by
Karla
• 0
3
votes
3
replies
1000
views
Optimizing CPM Calculation for Varied RNA-seq Data
edgeR
updated 14 months ago by
James W. MacDonald
68k • written 14 months ago by
Ahdee
▴ 60
0
votes
4
replies
1.0k
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 14 months ago by
Shaozhen
• 0
2,031 results • Page
1 of 41
Recent ...
Replies
Comment: incomplete imputation of missing values with LEA impute() function
by
Gordon Smyth
53k
Nadia, please tag your question with the name of the package ("LEA") that you are asking about. "impute" is the name of a different Biocond…
Comment: Sharing a collection of datasets in ExperimentHub
by
Aaron Lun
★ 29k
FYI the **scRNAseq** packages uses a non-ExperimentHub storage platform, see [here](https://github.com/ArtifactDB/gypsum-worker) for more d…
Comment: Error Loading Experiment from SimBenchData Package
by
ATpoint
★ 4.9k
Is the Seurat package installed? The error somewhat suggests that it could be related, and at https://www.bioconductor.org/packages/release…
Answer: Help with defining groups
by
Gordon Smyth
53k
Defining sample groups is separate to reading in the DIA-NN data, because the sample conditions and covariates are not necessarily coded in…
Comment: Sharing a collection of datasets in ExperimentHub
by
Peter Hickey
▴ 760
The [**scRNAseq**](https://bioconductor.org/packages/scRNAseq/) package ([source on GitHub](https://github.com/LTLA/scRNAseq)) might serve …
Votes
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
Answer: ordinary t from limma [was: How would I normally compare swirl?]
Comment: readDNAStringSet with non DNA characters
Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
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