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EDGER
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0
votes
3
replies
131
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What type of counts data to import for performing Isoform analysis in edgeR
limma
isoform
edgeR
salmon
R
updated 7 hours ago by
ATpoint
★ 4.5k • written 23 hours ago by
mohammedtoufiq91
▴ 10
7
votes
6
replies
641
views
ANOVA like approach of edgeR
edgeR
updated 1 day ago by
Gordon Smyth
52k • written 6 days ago by
Talip
▴ 10
3
votes
3
replies
467
views
Is it recommended to analyze different tissues together when using edgeR
DifferentialExpression
edgeR
updated 8 days ago by
Gordon Smyth
52k • written 8 days ago by
Lars Roed
• 0
2
votes
4
replies
1.5k
views
Differential Expression Analysis with unbalanced batches without replication between conditions
edgeR
BatchEffect
updated 17 days ago by
Gordon Smyth
52k • written 21 days ago by
Thili
• 0
1
vote
8
replies
1.0k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 24 days ago by
Adam Marstrand
• 0
0
votes
1
reply
427
views
single cell RNA seq pseudobulk batch correction
edgeR
updated 27 days ago by
James W. MacDonald
67k • written 27 days ago by
mairahtkhan
• 0
3
votes
3
replies
843
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 4 weeks ago by
Satoshi
• 0
0
votes
4
replies
573
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs
edgeR
RNASeqData
4 weeks ago
Philippine
• 0
4
votes
6
replies
1.1k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
5 weeks ago • updated 4 weeks ago
altintas.ali
• 0
1
vote
2
replies
295
views
edgeR 4.2.2 not compiled for Windows
edger
edgeR
6 weeks ago
Hongjian
• 0
3
votes
3
replies
283
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
6 weeks ago
Guido Hooiveld
★ 4.1k
4
votes
1
reply
309
views
edgeR 4.2.1 compression bug: a minimally reproducible snippet
edger
edgeR
updated 6 weeks ago by
Gordon Smyth
52k • written 7 weeks ago by
Hongjian
• 0
1
vote
1
reply
231
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 8 weeks ago by
ATpoint
★ 4.5k • written 8 weeks ago by
Pimpa
• 0
1
vote
2
replies
441
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 8 weeks ago by
Michael Love
43k • written 9 weeks ago by
Ben
▴ 10
0
votes
2
replies
337
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 8 weeks ago by
Gordon Smyth
52k • written 9 weeks ago by
blmurphy
• 0
1
vote
2
replies
313
views
Inconsistent result in edgeR
edgeR
10 weeks ago
hiroko.ohmiya
• 0
1
vote
1
reply
431
views
Help with design matrix for RNA-seq data using edgeR
edgeR
RNASeqData
updated 11 weeks ago by
Gordon Smyth
52k • written 12 weeks ago by
erik.burchard
• 0
2
votes
9
replies
792
views
Seeking Advice on Using edgeR and variancePartition for RNA-seq Data from Multiple Tissues and Patients
edgeR
variancePartition
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
Seymoo
• 0
0
votes
3
replies
484
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2
edgeR
DifferentialExpression
written 3 months ago by
dancingcow
• 0
1
vote
1
reply
623
views
Expected memory usage for analyzing large single-cell seq datasets (> 450,000 cells)?
BigData
Clustering
memory
SingleCellExperiment
edgeR
updated 3 months ago by
Aaron Lun
★ 28k • written 3 months ago by
Joseph
• 0
0
votes
1
reply
360
views
edgeR - design a complex matrix
design
model.matrix
edgeR
complexdesign
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
Karla
• 0
3
votes
3
replies
547
views
Optimizing CPM Calculation for Varied RNA-seq Data
edgeR
updated 3 months ago by
James W. MacDonald
67k • written 3 months ago by
Ahdee
▴ 50
0
votes
4
replies
560
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 4 months ago by
Shaozhen
• 0
1
vote
8
replies
934
views
Advice on appropriateness of an offset in glmGamPoi/edgeR
DESeq2
glmGamPoi
offset
edgeR
scRNAseq
updated 4 months ago by
Gordon Smyth
52k • written 5 months ago by
Alec
• 0
3
votes
5
replies
1.6k
views
Right way to pseudobulk donors across replicates in edgeR
pseudobulk
edgeR
DifferentialExpression
updated 5 months ago by
Reza Ghamsari
• 0 • written 8 months ago by
Jack S.
▴ 50
0
votes
4
replies
586
views
Single Cell Experiment : Differential Expression "List of length 0" - Help!
DifferentialExpression
scran
scater
SingleCellExperiment
edgeR
5 months ago
vohara4
• 0
3
votes
6
replies
820
views
Comapre two groups in edgeR- Coefficient and logFC explanation
edgeR
5 months ago
JG13
• 0
3
votes
2
replies
634
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
6 months ago
mohammedtoufiq91
▴ 10
3
votes
3
replies
684
views
How to correctly adjust for gender in edgeR
design
glm
edgeR
updated 6 months ago by
Gordon Smyth
52k • written 6 months ago by
Guillermo
• 0
1
vote
3
replies
625
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 6 months ago by
Gordon Smyth
52k • written 6 months ago by
Ashley
• 0
0
votes
1
reply
381
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
z_bahari
• 0
4
votes
2
replies
1.1k
views
Why does GSEA on edgeR results for randomized samples give highly significant p-values?
fgsea
edgeR
7 months ago • updated 6 months ago
gene_bioconductor
▴ 10
1
vote
2
replies
721
views
Citation for edgeR user guide
edgeR
written 7 months ago by
k___
▴ 30
4
votes
6
replies
982
views
FilterByExpr low counts with small sample size
edgeR
7 months ago
Jonathan
▴ 10
0
votes
2
replies
372
views
How to make DGEList and design matrix by edgeR package
DGEList
edgeR
7 months ago
prity6459
• 0
0
votes
3
replies
551
views
Finding DEGs by using edgeR
subsettingdata
edgeR
designmatrix
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
prity6459
• 0
5
votes
3
replies
842
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 6 months ago by
Gordon Smyth
52k • written 7 months ago by
Shaimaa Gamal
• 0
3
votes
3
replies
861
views
permutation test in edgeR
rna-seq
edgeR
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
Netanel
• 0
1
vote
3
replies
626
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
Simran
• 0
5
votes
6
replies
854
views
Not fully paired differential gene expression analysis
edgeR
DifferentialExpression
updated 7 months ago by
ATpoint
★ 4.5k • written 7 months ago by
nhaus
▴ 70
1
vote
3
replies
545
views
Lib sizes in the DGE object are different from original lib sizes
edgeR
DifferentialExpression
updated 7 months ago by
James W. MacDonald
67k • written 7 months ago by
Shaimaa Gamal
• 0
0
votes
2
replies
516
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
7 months ago
Marianna
▴ 20
0
votes
4
replies
831
views
Adjust RNA-seq counts (more than one batch variable) for plotting differentially expressed genes
ComBat
sva
edgeR
8 months ago
Lucy
▴ 60
3
votes
5
replies
1.2k
views
Differing results from plotMDS and PCAtools
edgeR
PCAtools
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
Fabian
• 0
0
votes
6
replies
705
views
Doing comparison of RNA-seq data using EdgeR
metaMSdata
edgeR
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
Faisal
• 0
0
votes
1
reply
566
views
Using splicing junction counts with edgeR/limma-voom
splicing
limma
edgeR
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
scoops_streams_06
• 0
6
votes
5
replies
1.7k
views
What benchmark should I use for setting the EdgeR filterByExpr min.count parameter?
edgeR
DifferentialExpression
updated 29 days ago by
Gordon Smyth
52k • written 8 months ago by
Jack S.
▴ 50
2
votes
3
replies
575
views
edgeR, 3 x 2 design with a batch effect and contrasts
design
edgeR
contrasts
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
JKim
• 0
0
votes
2
replies
563
views
RNAseq analysis
rnaseqGene
edgeR
DEGraph
DifferentialExpression
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
z_bahari
• 0
2
votes
2
replies
540
views
between lane normalization upper quantile
EDASeq
edgeR
8 months ago
elesofi
• 0
50 results • Page
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Comment: What type of counts data to import for performing Isoform analysis in edgeR
by
ATpoint
★ 4.5k
Probably nf-core did not run with bootstrap/gibbs replicates. Check the code. See salmon docs on how to turn that on.
Comment: What type of counts data to import for performing Isoform analysis in edgeR
by
mohammedtoufiq91
▴ 10
@atpoint Thank you. I did wanted to try using the `quant.sf` files from the `salmon` run by referring edgeR user guide example `4.6 Differe…
Comment: athPkgBuilder data source :missing probesets
by
bobihanigujjar
• 0
In this article, we'll explore the demand for unbranded BJJ Gis and the benefits of choosing unbranded Gis for customization. [does anyone …
Comment: Crossover design with limma
by
SamGG
▴ 360
IMHO, here are the missing annotations treatmentdrug_orderDrugPlac - treatmentplacebo_orderDrugPlac, # effect of drug for the Drug…
Answer: What type of counts data to import for performing Isoform analysis in edgeR
by
ATpoint
★ 4.5k
You don't need any of these files. You need the `catchSalmon` function which reads `quant.sf` and the inferential (Bootstrap/Gibbs) replica…
Votes
Comment: ANOVA like approach of edgeR
Comment: Crossover design with limma
Answer: DESeq2 normalization vs VST vs rlog
Comment: makeContrastsDream for Interaction
Answer: Limma- Print-tip loess normalisation-printer layout information does not match M
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