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RNASeqData
•
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0
votes
0
replies
53
views
Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2
RNASeqData
3 days ago
KR
• 0
0
votes
1
reply
97
views
combining raw counts and estimated counts
RNASeqData
GEO
TCGAbiolinks
updated 4 days ago by
O'Brien
• 0 • written 6 days ago by
Noor
• 0
1
vote
1
reply
241
views
DESeq2- merging technical vs biological replicates
DESeq2
RNASeqData
updated 27 days ago by
Michael Love
43k • written 4 weeks ago by
Nikita
• 0
0
votes
4
replies
635
views
RNA-seq: samples form different experiments, how to do DGEs?
DGEs
edgeR
RNASeqData
9 weeks ago • updated 8 weeks ago
Philippine
• 0
0
votes
2
replies
359
views
ESTMATE, MCPcounter, EPIC, xCell input
DESeq2
ESTIMATE
RNASeqData
MCPcounter
written 9 weeks ago by
Aleksandra
• 0
3
votes
11
replies
784
views
Highly DE genes but small count difference within conditions
RNASeq
DESeq2
RNASeqData
written 12 weeks ago by
bioshot.com
• 0
1
vote
1
reply
360
views
WGCNA
DEGselection
RNASeqData
WGCNA
updated 11 weeks ago by
Lluís Revilla Sancho
▴ 760 • written 11 weeks ago by
Nasim
• 0
1
vote
1
reply
263
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 3 months ago by
ATpoint
★ 4.6k • written 3 months ago by
Pimpa
• 0
1
vote
1
reply
468
views
Help with design matrix for RNA-seq data using edgeR
edgeR
RNASeqData
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
erik.burchard
• 0
1
vote
2
replies
1.1k
views
How to use compareCluster in ClusterProfiler?
RNASeqData
GSEA
clusterProfiler
written 3 months ago by
b8177
• 0
1
vote
2
replies
343
views
Questions in Regards to LFC shrinkage in DESeq2
Dseq2
RNASeqData
apeglm
4 months ago
Gordon
• 0
0
votes
3
replies
595
views
Error when trying to do different pairwise comparisons and lfcshrink than what's listed on resultsNames()
RNASeqData
DESeq2
RNASeq
DifferentialExpression
updated 5 months ago by
ATpoint
★ 4.6k • written 5 months ago by
Mark
• 0
0
votes
4
replies
608
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 5 months ago by
Shaozhen
• 0
1
vote
3
replies
989
views
Combining two bulk RNA seq datasets
RNASeqData
DESeq2
Normalization
6 months ago
Tanvi
• 0
1
vote
3
replies
589
views
Including infectivity information to RNA-Seq analysis
RNASeqData
DESeq2
RNA-Seq
RNASeq
updated 6 months ago by
James W. MacDonald
67k • written 6 months ago by
Ben
▴ 50
0
votes
1
reply
376
views
How to identify transcripts/genes with differential kinetics in two genotypes, using time-course RNA seq data?
RNASeqData
DESeq2
DifferentialExpression
updated 6 months ago by
James W. MacDonald
67k • written 6 months ago by
ZhuYi
• 0
0
votes
1
reply
379
views
ReactomeGSA for RNASeq
ReactomeGSA
BatchEffect
RNASeqData
updated 3 months ago by
jg
▴ 20 • written 7 months ago by
Shaimaa Gamal
• 0
0
votes
1
reply
401
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 8 months ago by
Gordon Smyth
52k • written 8 months ago by
z_bahari
• 0
0
votes
1
reply
337
views
Brachypodium distachyon db
EnrichmentBrowser
BrachypodiumDB
RNASeqData
updated 9 months ago by
James W. MacDonald
67k • written 9 months ago by
Leyre
• 0
3
votes
5
replies
1.2k
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 10 months ago by
Michael Love
43k • written 10 months ago by
sap275
▴ 10
0
votes
0
replies
519
views
Differential expression analysis between different species
DEG
RNASeqData
DifferentialExpression
SCBN
RNASeq
10 months ago
Abril
• 0
1
vote
2
replies
1.8k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 20 months ago by
informatician
• 0
1
vote
3
replies
712
views
DESeq2 analysis on time-series RNA experiment
RNASeqData
DESeq2
RNASeq
11 months ago
island
• 0
0
votes
2
replies
529
views
ConsensusClusterPlus dosesn't make an output file
ConsensusClusterPlus
RNASeqData
11 months ago
Diana
• 0
0
votes
1
reply
758
views
WGCNA Soft Power
Normalization
DESeq2
Network
RNASeqData
updated 11 months ago by
ATpoint
★ 4.6k • written 11 months ago by
Ömür Koray
• 0
5
votes
6
replies
1.0k
views
Monotonic genes in results of differential gene expression analysis
DifferentialExpression
rnaseqGene
RNASeqData
11 months ago
Shaimaa Gamal
• 0
1
vote
1
reply
661
views
Deseq2 -Model matrix not full rank error: A complex case
RNASeqData
DifferentialExpression
DESeq2
updated 12 months ago by
ATpoint
★ 4.6k • written 12 months ago by
kubracelikbas4
• 0
0
votes
2
replies
758
views
StringDB: Cannot use payload mechanism to colour nodes
RNASeqData
STRINGdb
DifferentialExpression
GraphAndNetwork
Network
12 months ago
Luca
• 0
0
votes
1
reply
1.0k
views
Interaction terms in DESeq2
RNASeqData
RnaSeqSampleSize
DESeq2
updated 12 months ago by
James W. MacDonald
67k • written 12 months ago by
Mimi
• 0
1
vote
2
replies
788
views
RNASeq time course analysis, missing time points for control
RNASeqData
RnaSeqSampleSize
RNASeq
RNASeqPower
13 months ago
drewhaydensteele
• 0
1
vote
1
reply
651
views
Differential gene expression analysis
RNASeqData
RNAseq123
updated 13 months ago by
ATpoint
★ 4.6k • written 13 months ago by
kaurprab
• 0
1
vote
2
replies
1.2k
views
Different results from blockwiseModules and blockwiseConsensusModules individual TOM
RNASeqData
WGCNA
consensusnetworks
13 months ago • updated 12 months ago
Levi
• 0
0
votes
3
replies
1.6k
views
DESeq: Error in DESeqDataSet(se, design = design, ignoreRank) : some values in assay are negative
RNASeqData
DESeq2
13 months ago
Oenone
• 0
3
votes
4
replies
976
views
Is it appropriate to apply linear mixed models to Voom transformed data?
stats
r
RNASeqData
limma
voom
13 months ago
pl23
• 0
0
votes
1
reply
553
views
DESeq2 and determining effects of treatment on a priori/candidate genes from a bulk-RNAseq experiment
RNASeqData
DifferentialExpression
DESeq2
updated 13 months ago by
Michael Love
43k • written 13 months ago by
Pez
▴ 10
2
votes
2
replies
715
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
13 months ago
Manthos
• 0
0
votes
1
reply
653
views
DESeq2 interaction between one continuous variable and one categorical variable
RNASeqData
DESeq2
updated 13 months ago by
Wolfgang Huber
★ 13k • written 13 months ago by
meroy
• 0
0
votes
2
replies
698
views
Is my data too noisy for DESeq? Should I model noise as unwanted variation?
RNASeqData
DESeq2
13 months ago
Ben
▴ 10
0
votes
2
replies
1.7k
views
MA plot for DESEq2 analysis
RNASeqData
DESeq2
14 months ago • updated 13 months ago
RHEA
• 0
2
votes
3
replies
1.8k
views
Two factor design including interaction effect with DESeq2 in R
DESeq2
RNASeqData
deseq2
ExperimentalDesign
updated 14 months ago by
swbarnes2
★ 1.4k • written 14 months ago by
Sam
• 0
1
vote
1
reply
738
views
Multiple comparison using DESeq2
RNASeqData
RNASeq
DESeq2
updated 14 months ago by
James W. MacDonald
67k • written 14 months ago by
fuqichen1
• 0
1
vote
7
replies
1.3k
views
DuplicateCorrelation() block by duplicate sample or by cell line ?
variancePartition
RNASeqData
limma
BulkRNAseq
RNASeq
updated 13 months ago by
Gordon Smyth
52k • written 14 months ago by
Gimena
• 0
0
votes
1
reply
688
views
Comparing RNASeq data from multiple studies
RNASeqData
RnaSeqSampleSize
RNASeqPower
RNASeq
updated 15 months ago by
Basti
▴ 780 • written 15 months ago by
Luke
• 0
2
votes
4
replies
1.2k
views
Deseq2 calculated log2foldchange not consistent with RPM in IP-small RNA-seq
RNASeqData
DifferentialExpression
SmallRNAData
DESeq2
15 months ago
Shihui
• 0
0
votes
4
replies
1.1k
views
filtering out lowly expressed genes by groups
RNASeqData
DESeq2
updated 15 months ago by
James W. MacDonald
67k • written 15 months ago by
sohail.mbio
▴ 10
3
votes
3
replies
2.5k
views
Differential expression analysis with only FPKM matrix available from total newbie in R
FPKM
RNASeqData
DEAnalysis
limma
15 months ago
Ethan Nguyen
• 0
1
vote
1
reply
1.2k
views
Can I transform already normalized rna-seq data with Z-score?
Z-score
RNASeqData
Deseq
DESeq2
updated 15 months ago by
James W. MacDonald
67k • written 15 months ago by
khaserdene99
• 0
0
votes
2
replies
820
views
RSubread DESeq2
RNASeqData
DifferentialExpression
DESeq2
15 months ago
Beyza
• 0
1
vote
1
reply
736
views
bulk RNA-Seq batch correction
RNASeqData
rnaseqcomp
RNASeq
updated 15 months ago by
ATpoint
★ 4.6k • written 15 months ago by
jg0428
• 0
0
votes
0
replies
509
views
How to use erccdashboard with erccmix="Single" design
RNASeqData
erccdashboard
16 months ago • updated 15 months ago
adi.rotem
• 0
50 results • Page
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Recent ...
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Answer: DESeq error: counts matrix should be numeric, currently it has mode: character
by
ATpoint
★ 4.6k
R thinks your data are characters (e.g. "1", "2", instead of 1, 2) so given that your `counts_data` indeed only contains counts, do: `mo…
Comment: Any tools to convert .gff to .gtf format
by
ControlF5
• 0
Yes, the Appsierra team recently built a tool to convert .gff to .gtf format. [ControlF5][1] is providing the best software testing service…
Comment: EuroBioC2024 abstract submission is open!
by
Alan Downey
• 0
EuroBioC2024 has opened its abstract submission process, presenting an exciting opportunity for researchers to contribute to groundbreaking…
Comment: ChAMP.DMR error - Error in makeGenomicRatioSetFromMatrix
by
O'Brien
• 0
Consult the ChAMP.DMR documentation for more information on supported array types and expected data formats: https://bioconductor.org/packa…
Answer: combining raw counts and estimated counts
by
O'Brien
• 0
It is possible to combine raw counts from TCGA and estimated counts from GEO datasets (Kalisto pseudoalignment) while mitigating batch effe…
Votes
A: GWAS analysis with Illumina HumanOmni5-4 BeadChip - recommended software and wor
A: GWAS analysis with Illumina HumanOmni5-4 BeadChip - recommended software and wor
A: RNA-seq Normalisation - normalise all samples in experiment or only the ones use
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
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