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rnaseq
•
reset
1
vote
4
replies
3.1k
views
Should I perform batch correction of normalized RNA-seq counts prior to GSVA?
GSVA
GSVAdata
RNAseq
19 months ago
Lucy
▴ 60
0
votes
4
replies
1.2k
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 19 months ago by
Shaozhen
• 0
0
votes
0
replies
827
views
Cell type deconvolution of RNAseq data- Create signature matrix of cells
deconvolution
rnaseqGene
RNAseq
20 months ago
JG13
• 0
0
votes
1
reply
799
views
Significant genes in DESeq2 analysis
DESeq2
RNASeq
updated 20 months ago by
James W. MacDonald
68k • written 20 months ago by
cbio
• 0
1
vote
9
replies
4.2k
views
Most enriched pathway is not expressed when modifying universe parameter
DESeq2
clusterProfiler
Pathways
RNASeq
org.Mm.eg.db
updated 20 months ago by
James W. MacDonald
68k • written 20 months ago by
adrip
▴ 10
1
vote
3
replies
1.1k
views
Including infectivity information to RNA-Seq analysis
RNASeqData
DESeq2
RNA-Seq
RNASeq
updated 20 months ago by
James W. MacDonald
68k • written 20 months ago by
Ben
▴ 50
2
votes
2
replies
1.1k
views
How to remove batch effect to combine RNAseq data from 2 different experiments?
BatchQC
BatchEffect
RNASeq
updated 21 months ago by
jessica.anderson
▴ 10 • written 21 months ago by
ttt
• 0
0
votes
6
replies
2.5k
views
Using featureCounts with SAF annotation file gives "Error: only GTF files contain additional attributes"
RNASeq
Rsubread
21 months ago
atan
• 0
3
votes
2
replies
1.4k
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
21 months ago
mohammedtoufiq91
▴ 10
0
votes
0
replies
727
views
scRNA-seq data trained model can be used for predictions on bulk RNA-seq data?
RNASeq
scRNAseq
21 months ago
Bibi
• 0
10
votes
11
replies
3.4k
views
SGSeq: moving toward diffex from SGSeq analysis
sgseq
rnaseq
updated 21 months ago by
Sara
• 0 • written 8.5 years ago by
Sylvain Foisy
▴ 80
1
vote
3
replies
1.2k
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 21 months ago by
Gordon Smyth
53k • written 21 months ago by
Ashley
• 0
0
votes
3
replies
1.9k
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 22 months ago by
ATpoint
★ 5.0k • written 22 months ago by
james.zhang20
• 0
0
votes
1
reply
949
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 22 months ago by
Michael Love
43k • written 22 months ago by
kcarey
• 0
0
votes
2
replies
871
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 22 months ago by
taur.vil
• 0
0
votes
2
replies
1.4k
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
22 months ago
catv
• 0
5
votes
3
replies
1.8k
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 21 months ago by
Gordon Smyth
53k • written 22 months ago by
Shaimaa Gamal
• 0
0
votes
1
reply
1.7k
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 22 months ago by
Michael Love
43k • written 22 months ago by
09191919
• 0
0
votes
1
reply
1.1k
views
Can you find the correct transcript in scRNA seq data
scRNAseq
RNAseq
updated 22 months ago by
ATpoint
★ 5.0k • written 22 months ago by
Merav
• 0
0
votes
2
replies
2.4k
views
Filtering and Reference Level for DESeq2
DESeq2
GeneFiltering
RNAseq
23 months ago
kcarey
• 0
2
votes
2
replies
938
views
Question about extracting co-efficients for pairwise comparisons in edgeR
limma
fit
design
RNASeq
edgeR
23 months ago
Sabiha
▴ 20
0
votes
2
replies
4.1k
views
RNA sequencing raw counts gene filtering
DESeq2
limma
RNAseq
23 months ago
kcarey
• 0
1
vote
4
replies
1.0k
views
DESeq2 Replicates
DESeq2
RNASeq
23 months ago
kcarey
• 0
1
vote
3
replies
1.2k
views
DESEQ2 Batch Effect
DESeq2
BatchEffect
RNASeq
updated 24 months ago by
swbarnes2
★ 1.4k • written 24 months ago by
sap275
▴ 10
0
votes
4
replies
1.1k
views
Interactions in batches of varying size with different number of replicates using limma
limma
RNASeq
DifferentialExpression
24 months ago
mperalc
• 0
0
votes
2
replies
1.3k
views
Error in featureCounts matrix
GenomeWideAssociation
countsimQC
RNASeq
updated 24 months ago by
Gordon Smyth
53k • written 24 months ago by
ryann
• 0
3
votes
5
replies
1.9k
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 24 months ago by
Michael Love
43k • written 2.0 years ago by
sap275
▴ 10
2
votes
1
reply
819
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 24 months ago by
Michael Love
43k • written 24 months ago by
sap275
▴ 10
3
votes
7
replies
2.2k
views
Average biological replicates in RNA-seq
Mfuzz
RNASeq
2.0 years ago • updated 5 months ago
boczniak767
▴ 740
0
votes
0
replies
847
views
Differential expression analysis between different species
DEG
RNASeqData
DifferentialExpression
SCBN
RNASeq
2.0 years ago
Abril
• 0
2
votes
2
replies
1.9k
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
2.0 years ago
jackafiore
• 0
1
vote
2
replies
2.7k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 2.8 years ago by
informatician
• 0
0
votes
4
replies
1.1k
views
Ballgown error in scan for c.tab files
ballgown
RNASeq
2.1 years ago
candacenewcome
• 0
1
vote
3
replies
1.3k
views
DESeq2 analysis on time-series RNA experiment
RNASeqData
DESeq2
RNASeq
2.1 years ago
island
• 0
1
vote
3
replies
1.4k
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics
Normalization
RNASeq
DESeq2
written 2.1 years ago by
georgii.vdovin
• 0
0
votes
1
reply
722
views
Error in GDCRNATools
ceRNA
GDCRNATools
RNASeq
updated 2.1 years ago by
Ram
▴ 220 • written 2.1 years ago by
Matvii Mykhailichenko
• 0
2
votes
4
replies
3.2k
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
2.1 years ago
Sabiha
▴ 20
4
votes
6
replies
1.8k
views
filtering lowly expressed reads
RNASeq
limma
DifferentialExpression
Preprocessing
updated 2.0 years ago by
Gordon Smyth
53k • written 2.1 years ago by
Shaimaa Gamal
• 0
3
votes
1
reply
968
views
Dataset imbalance
RNASeq
DifferentialExpression
RnaSeqSampleSizeData
RnaSeqSampleSize
updated 2.1 years ago by
Gordon Smyth
53k • written 2.1 years ago by
Shaimaa Gamal
• 0
0
votes
3
replies
1.7k
views
dittobarplot Var error
RNASeq
ditto
dittoSeq
SingleR
written 2.1 years ago by
Fayrouz
• 0
0
votes
1
reply
908
views
DESeq Design for Time-Series Analysis
RNASeq
DESeq2
timecourse
2.1 years ago
WS
• 0
2
votes
3
replies
4.2k
views
Using STAR's readspergene.tab.out outputs to make gene-level count matrix for DESeq2 using tximport
STAR
tximport
RNASeq
DESeq2
updated 2.2 years ago by
swbarnes2
★ 1.4k • written 2.2 years ago by
Bo
• 0
1
vote
2
replies
1.4k
views
How to perform longitudinal analysis using EdgeR
StatisticalMethod
FoldChange
edgeR
RNASeq
2.2 years ago
Sabiha
▴ 20
1
vote
2
replies
1.3k
views
RNASeq time course analysis, missing time points for control
RNASeqData
RnaSeqSampleSize
RNASeq
RNASeqPower
2.2 years ago
drewhaydensteele
• 0
12
votes
5
replies
5.7k
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR
limma
voom
RNASeq
muscat
updated 8 months ago by
Geoff
• 0 • written 2.2 years ago by
annikagable
• 0
0
votes
3
replies
1.4k
views
'Subtracting' counts on basis of Negative control media and water samples
limma
edgeR
RNASeq
DESeq2
2.3 years ago • updated 2.2 years ago
Karthik
• 0
1
vote
1
reply
883
views
Using DESeq2 statistical framework with to identify differentially expressed loci instead of genes
rnaseq
DESeq2
updated 2.2 years ago by
Michael Love
43k • written 2.3 years ago by
pl23
• 0
2
votes
3
replies
1.7k
views
DESeq2 estimateSizeFactors iterate takes too long
RNASeq
DESeq2
2.3 years ago • updated 2.2 years ago
Karthik
• 0
2
votes
2
replies
1.2k
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
2.3 years ago
Manthos
• 0
2
votes
4
replies
1.6k
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom
RNASeq
limma
2.3 years ago
Tyler Sagendorf
▴ 10
1,414 results • Page
2 of 29
Recent ...
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Answer: Reproducibility of code in various Bioconductor versions
by
Gordon Smyth
53k
I'm not familiar with the packages you mention, but the whole reason why Bioconductor has versions is that code and annotation change. Code…
Comment: Question on processing compound protein groups in limpa
by
JKim
• 0
Many thanks. I appreciate your advice.
Answer: Question on processing compound protein groups in limpa
by
Gordon Smyth
53k
Different people have different ways of dealing with compound protein groups. You could just leave them in the analysis, in which case limp…
Answer: Correct usage of limpa for protein input
by
Gordon Smyth
53k
limpa can work on protein-level quantifications, but they must be intensity-based and must be on the log scale. From what I read (<https://…
Comment: A problem occurs when running the monocle2
by
a2a2hset32aa
• 0
Troubleshooting monocle2 errors in R 4.5.1 often points to package incompatibilities, particularly with newer versions of igraph. For resea…
Votes
Answer: Question on processing compound protein groups in limpa
Answer: Correct usage of limpa for protein input
Answer: Correct usage of limpa for protein input
Answer: Correct usage of limpa for protein input
A: Interpretaion of dendrogram height in WGCNA?
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