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rnaseq
•
reset
0
votes
6
replies
2.1k
views
Using featureCounts with SAF annotation file gives "Error: only GTF files contain additional attributes"
RNASeq
Rsubread
16 months ago
atan
• 0
3
votes
2
replies
1.1k
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
16 months ago
mohammedtoufiq91
▴ 10
0
votes
0
replies
593
views
scRNA-seq data trained model can be used for predictions on bulk RNA-seq data?
RNASeq
scRNAseq
16 months ago
Bibi
• 0
10
votes
11
replies
3.0k
views
SGSeq: moving toward diffex from SGSeq analysis
sgseq
rnaseq
updated 16 months ago by
Sara
• 0 • written 8.1 years ago by
Sylvain Foisy
▴ 80
1
vote
3
replies
1.0k
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 16 months ago by
Gordon Smyth
53k • written 16 months ago by
Ashley
• 0
0
votes
3
replies
1.4k
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 16 months ago by
ATpoint
★ 4.9k • written 16 months ago by
james.zhang20
• 0
0
votes
1
reply
797
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 17 months ago by
Michael Love
43k • written 17 months ago by
kcarey
• 0
0
votes
2
replies
726
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 17 months ago by
taur.vil
• 0
0
votes
2
replies
1.1k
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
17 months ago
catv
• 0
5
votes
3
replies
1.5k
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 16 months ago by
Gordon Smyth
53k • written 17 months ago by
Shaimaa Gamal
• 0
0
votes
1
reply
1.2k
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 17 months ago by
Michael Love
43k • written 17 months ago by
09191919
• 0
0
votes
1
reply
904
views
Can you find the correct transcript in scRNA seq data
scRNAseq
RNAseq
updated 17 months ago by
ATpoint
★ 4.9k • written 17 months ago by
Merav
• 0
0
votes
2
replies
1.9k
views
Filtering and Reference Level for DESeq2
DESeq2
GeneFiltering
RNAseq
18 months ago
kcarey
• 0
2
votes
2
replies
756
views
Question about extracting co-efficients for pairwise comparisons in edgeR
limma
fit
design
RNASeq
edgeR
18 months ago
Sabiha
▴ 20
0
votes
2
replies
3.0k
views
RNA sequencing raw counts gene filtering
DESeq2
limma
RNAseq
18 months ago
kcarey
• 0
1
vote
4
replies
850
views
DESeq2 Replicates
DESeq2
RNASeq
18 months ago
kcarey
• 0
1
vote
3
replies
998
views
DESEQ2 Batch Effect
DESeq2
BatchEffect
RNASeq
updated 18 months ago by
swbarnes2
★ 1.4k • written 18 months ago by
sap275
▴ 10
0
votes
4
replies
963
views
Interactions in batches of varying size with different number of replicates using limma
limma
RNASeq
DifferentialExpression
19 months ago
mperalc
• 0
0
votes
2
replies
1.1k
views
Error in featureCounts matrix
GenomeWideAssociation
countsimQC
RNASeq
updated 19 months ago by
Gordon Smyth
53k • written 19 months ago by
ryann
• 0
3
votes
5
replies
1.6k
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 18 months ago by
Michael Love
43k • written 19 months ago by
sap275
▴ 10
2
votes
1
reply
696
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 19 months ago by
Michael Love
43k • written 19 months ago by
sap275
▴ 10
3
votes
7
replies
1.8k
views
Average biological replicates in RNA-seq
Mfuzz
RNASeq
19 months ago • updated 10 days ago
boczniak767
▴ 740
0
votes
0
replies
723
views
Differential expression analysis between different species
DEG
RNASeqData
DifferentialExpression
SCBN
RNASeq
19 months ago
Abril
• 0
2
votes
2
replies
1.7k
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
19 months ago
jackafiore
• 0
1
vote
2
replies
2.4k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 2.4 years ago by
informatician
• 0
0
votes
4
replies
954
views
Ballgown error in scan for c.tab files
ballgown
RNASeq
20 months ago • updated 19 months ago
candacenewcome
• 0
1
vote
3
replies
1.1k
views
DESeq2 analysis on time-series RNA experiment
RNASeqData
DESeq2
RNASeq
20 months ago • updated 19 months ago
island
• 0
1
vote
3
replies
1.3k
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics
Normalization
RNASeq
DESeq2
written 20 months ago by
georgii.vdovin
• 0
0
votes
1
reply
614
views
Error in GDCRNATools
ceRNA
GDCRNATools
RNASeq
updated 20 months ago by
Ram
▴ 220 • written 20 months ago by
Matvii Mykhailichenko
• 0
2
votes
4
replies
2.0k
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
20 months ago
Sabiha
▴ 20
4
votes
6
replies
1.5k
views
filtering lowly expressed reads
RNASeq
limma
DifferentialExpression
Preprocessing
updated 19 months ago by
Gordon Smyth
53k • written 20 months ago by
Shaimaa Gamal
• 0
3
votes
1
reply
798
views
Dataset imbalance
RNASeq
DifferentialExpression
RnaSeqSampleSizeData
RnaSeqSampleSize
updated 20 months ago by
Gordon Smyth
53k • written 20 months ago by
Shaimaa Gamal
• 0
0
votes
3
replies
1.5k
views
dittobarplot Var error
RNASeq
ditto
dittoSeq
SingleR
written 20 months ago by
Fayrouz
• 0
0
votes
1
reply
729
views
DESeq Design for Time-Series Analysis
RNASeq
DESeq2
timecourse
20 months ago
WS
• 0
2
votes
3
replies
3.4k
views
Using STAR's readspergene.tab.out outputs to make gene-level count matrix for DESeq2 using tximport
STAR
tximport
RNASeq
DESeq2
updated 21 months ago by
swbarnes2
★ 1.4k • written 21 months ago by
Bo
• 0
1
vote
2
replies
1.2k
views
How to perform longitudinal analysis using EdgeR
StatisticalMethod
FoldChange
edgeR
RNASeq
21 months ago
Sabiha
▴ 20
1
vote
2
replies
1.1k
views
RNASeq time course analysis, missing time points for control
RNASeqData
RnaSeqSampleSize
RNASeq
RNASeqPower
21 months ago
drewhaydensteele
• 0
12
votes
5
replies
4.8k
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR
limma
voom
RNASeq
muscat
updated 3 months ago by
Geoff
• 0 • written 22 months ago by
annikagable
• 0
0
votes
3
replies
1.2k
views
'Subtracting' counts on basis of Negative control media and water samples
limma
edgeR
RNASeq
DESeq2
22 months ago
Karthik
• 0
1
vote
1
reply
720
views
Using DESeq2 statistical framework with to identify differentially expressed loci instead of genes
rnaseq
DESeq2
updated 22 months ago by
Michael Love
43k • written 22 months ago by
pl23
• 0
2
votes
3
replies
1.4k
views
DESeq2 estimateSizeFactors iterate takes too long
RNASeq
DESeq2
22 months ago
Karthik
• 0
2
votes
2
replies
1.0k
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
22 months ago
Manthos
• 0
2
votes
4
replies
1.3k
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom
RNASeq
limma
22 months ago
Tyler Sagendorf
▴ 10
0
votes
1
reply
769
views
DESeq2 model for two genome system
RNASeq
DESeq2
updated 22 months ago by
Michael Love
43k • written 23 months ago by
Sean
• 0
0
votes
2
replies
1.2k
views
TCGA counts to TPM from recount3 do not seem to right
recount3
TCGA
RNASeq
updated 22 months ago by
Leonardo Collado Torres
★ 1.1k • written 23 months ago by
tangming2005
▴ 200
0
votes
9
replies
3.1k
views
DESeq2 result really different when only use some sample vs all sample
deseq2
rnaseq
updated 22 months ago by
blowfish
• 0 • written 7.9 years ago by
bharata1803
▴ 60
1
vote
4
replies
1.4k
views
controling for batch effect while preserving variation within repeated measures that unevenly distributed across batches
miRNA
BatchEffect
RNASeq
limma
22 months ago
Wending
• 0
0
votes
0
replies
794
views
Include or exclude N/A genes (those without any KEGG annotation) when running Wilcox test?
KEGGREST
KEGG
RNASeq
23 months ago
mch
• 0
5
votes
2
replies
2.1k
views
DecideTest vs topTAGs in edgeR
RNAseq
StatisticalMethod
edgeR
updated 23 months ago by
Gordon Smyth
53k • written 23 months ago by
alvaroperona1
▴ 10
0
votes
6
replies
2.2k
views
DESeq2 Normalization with 4 Groups
Normalization
RNASeq
DESeq2
23 months ago
erika.maldonado-rosado
• 0
1,408 results • Page
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Comment: DiffBind - question on the use of filtered or unfiltered input data
by
deltaexecutor4
• 0
FFH4X is a [Free Fire mod][1] menu APK that offers cheats like auto headshot, aimbot, ESP, wallhack, and unlimited skins. Players use it to…
Comment: GO.db and others package Download failure
by
James W. MacDonald
68k
Also, you never need to use anything but `BiocManager::install` to install any package, which has the beneficial side effect of ensuring th…
Comment: GO.db and others package Download failure
by
James W. MacDonald
68k
Yes. Your packages have odd Y version numbers (where the version is X.Y.Z). That means they are devel packages (release versions have even …
Comment: GO.db and others package Download failure
by
Skyler
• 0
Am I using Bioc-devel?
Comment: GO.db and others package Download failure
by
James W. MacDonald
68k
Is there a reason to use Bioc-devel? If not, you should be using the release version (which I am, and which installs `GO.db` without compla…
Votes
Comment: readDNAStringSet with non DNA characters
Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
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