Log In
Sign Up
about
faq
Ask a question
Latest
News
Jobs
Tutorials
Tags
Users
New Post
Latest
News
Jobs
Tutorials
Tags
Users
Log In
Sign Up
About
Limit
all time
today
this week
this month
this year
Unanswered
All posts
Sort
Update
Answers
Bookmarks
Creation
Replies
Rank
Views
Votes
Showing :
RNAseq
•
reset
0
votes
0
replies
711
views
how to create PHENO_DATA
RNASeq
RNASeqData
RNASeqRData
3.1 years ago
marymshahi2021
• 0
0
votes
2
replies
2.4k
views
maSigPro: how to extract the genes in the same cluster
rnaseq
updated 3.1 years ago by
oseias.rf.junior
• 0 • written 9.5 years ago by
simonjean434
▴ 10
1
vote
2
replies
768
views
how to plot the union of the 100 most expressed genes
DESeq2
RNASEQ
updated 3.1 years ago by
ATpoint
★ 5.0k • written 3.1 years ago by
reem
• 0
0
votes
1
reply
1.4k
views
Correlation between two different datasets: between results of RNAseq and absence/presence of Type3 Secretion System
RNASeq
Correlation
DESeq2
updated 3.2 years ago by
Michael Love
43k • written 3.2 years ago by
keshav.prasad.gubbi
• 0
2
votes
8
replies
3.0k
views
Error in Create switchAnalyzeRlist
AlternativeSplicing
RNASeq
IsoformSwitchAnalyzeR
gtf
written 3.2 years ago by
bpshtiwan
▴ 10
0
votes
2
replies
2.6k
views
Error with TCGA package - TCGAbiolinks
TCGAbiolinks
RNASeq
CancerData
updated 3.2 years ago by
Tiago C. Silva
▴ 270 • written 3.3 years ago by
JAcky
• 0
0
votes
0
replies
790
views
DEXSeq aggregated genes/exons result in NA values
RNASeq
R
DEXSeq
3.2 years ago
osieman52
• 0
4
votes
5
replies
3.4k
views
Which is the best type of data for correlation or survival analysis
TPM
RNASeq
RSEM
DESeq2
updated 3.2 years ago by
Michael Love
43k • written 3.2 years ago by
Yang Shi
▴ 10
1
vote
1
reply
800
views
Handling heterogeneous data in DESeq2: multiple covariates to control against
RNASeq
DESeq2
updated 3.2 years ago by
ATpoint
★ 5.0k • written 3.2 years ago by
seeta_rajpara
• 0
1
vote
5
replies
3.1k
views
DESeq2 outlier options and LFC Shrinkage
DESeq2
RNASeq
RnaSeqSampleSize
apeglm
updated 3.2 years ago by
Michael Love
43k • written 3.2 years ago by
Riley
• 0
0
votes
6
replies
2.2k
views
Is DESeq2 generating false postives when changing different pre-filtering thresholds?
RNASeq
DEG
DESeq2
3.2 years ago
Weiqian
▴ 10
0
votes
0
replies
591
views
Can I loosely say that a gene is expressed by simply look at the gene count from RNA-Seq?
RNASeqData
RNASeq
3.2 years ago
Pang
• 0
1
vote
1
reply
1.3k
views
Filtering Orthologus Genes Before or After TMM normalization?
RNASeq
Normalization
TMM
edgeR
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
e.g.w.miller
▴ 10
0
votes
2
replies
1.4k
views
IsoformSwitchAnalyzeR package on mouse data
rnaseq
IsoformSwitchAnalyzeR
updated 3.2 years ago by
k.vitting.seerup
▴ 120 • written 3.2 years ago by
Ryan
• 0
0
votes
1
reply
1.4k
views
Job:
'Omics data analyst for HPCBio, University of Illinois
Metagenomics
GenomicVariation
Genomics
RNASeq
ChIPSeq
3.2 years ago
Jenny Drnevich
★ 2.0k
5
votes
6
replies
2.6k
views
Removing the duplicated genes before limma::voom by using limma::avereps
RSEM
avereps
voom
limma
RNASeq
3.2 years ago
Yang Shi
▴ 10
1
vote
5
replies
1.3k
views
DeSeq2 Matched Analysis
RNASeq
DESeq2
3.2 years ago
Andrea
• 0
3
votes
4
replies
1.5k
views
Can I use limma::avereps to remove dupicated genes before conduct DEG based on RSEM Expected_count data?
limma
RNASeq
Expected_count
DESeq2
3.2 years ago
Yang Shi
▴ 10
1
vote
2
replies
3.0k
views
DEseq2 anlysis - LfcThreshold and Lfcshrink options
lfcshrink
DESeq2
RNASeq
lfcthreshold
3.2 years ago
ClaudiaE
• 0
1
vote
0
replies
887
views
Which algorithm would you use for the cell-cell communication analysis of bulk RNA seq datasets?
RNASeq
3.3 years ago
curiousmind007
▴ 10
3
votes
4
replies
3.5k
views
Why Negative value from GSVA
GSVA
RNASeq
edgeR
3.3 years ago
Yang Shi
▴ 10
5
votes
8
replies
27k
views
Confused about the CPM and TMM normalization in edgeR
RNASeq
R
edgeR
updated 14 months ago by
Ahdee
▴ 60 • written 3.3 years ago by
Mohamed
▴ 30
2
votes
2
replies
1.3k
views
Can't load UCSC transcript IDs
RNASeq
updated 3.3 years ago by
Guido Hooiveld
★ 4.1k • written 3.3 years ago by
becker.jemima
• 0
2
votes
4
replies
2.3k
views
deseq2 lfc shrinkage with few DEGs
RNASeq
DESeq2
updated 6 months ago by
Michael Love
43k • written 3.3 years ago by
Victor
▴ 10
3
votes
3
replies
1.3k
views
Nested analysis or Collapsed-replicate analysis?
RNASeq
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
giulia.lopatriello
• 0
0
votes
1
reply
949
views
lfc Shrinkage with multiple comparison groups
RNAseq
lfcShrinkage
DESeq2
updated 3.3 years ago by
ATpoint
★ 5.0k • written 3.3 years ago by
m.lorgen.06
• 0
0
votes
1
reply
754
views
Nested analysis or Collapsed-replicate analysis?
RNASeq
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
giulia.lopatriello
• 0
1
vote
1
reply
1.1k
views
RNA-Seq normalization and batch effect removal
RNASeq
BatchEffect
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
Alexandre
• 0
1
vote
1
reply
1.1k
views
NOIseq Probability column
RNAseq
RNASeqData
NOISeq
3.3 years ago
Pratibha
▴ 10
9
votes
3
replies
9.5k
views
Limma vs deseq2 results
limma
RNASeq
R
3.4 years ago
k___
▴ 30
3
votes
3
replies
2.4k
views
Question on RNA-Seq single sample data analysis in EdgeR
R
edgeR
limma
RNASeq
Normalization
updated 3.4 years ago by
Gordon Smyth
53k • written 3.4 years ago by
mohammedtoufiq91
▴ 10
3
votes
9
replies
3.2k
views
Warnings when using "ashr" in lfcshrinkage for DESeq2
RNASeq
DESeq2
updated 2.8 years ago by
ayabalbaa1990
• 0 • written 3.4 years ago by
andrebolerbarros
▴ 20
1
vote
2
replies
2.2k
views
Looking for a package that compares my RNA seq data to find published dataset with a similar expression pattern
RNASeq
written 3.4 years ago by
curiousmind007
▴ 10
1
vote
1
reply
2.1k
views
Using featureCounts and downloading Rsubread
featureCounts
RNASeq
Rsubread
R
updated 3.4 years ago by
Gordon Smyth
53k • written 3.4 years ago by
margo
• 0
1
vote
2
replies
1.7k
views
How to properly use DESeq2 for an experiment
ExperimentalDesign
RNASeq
DifferentialExpression
DESeq2
updated 3.4 years ago by
Michael Love
43k • written 3.4 years ago by
Aurélien
• 0
3
votes
11
replies
4.3k
views
RNA-seq: compare two technical replicates
RNASeq
updated 3.4 years ago by
Gordon Smyth
53k • written 3.5 years ago by
Marianna
▴ 20
0
votes
2
replies
985
views
edgeR
rnaseq
edgeR
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
edusbioinfo
• 0
0
votes
3
replies
6.7k
views
Best way to do RNA-seq analysis for a small number of samples obtained from 2 different batches
RNASeq
updated 3.5 years ago by
swbarnes2
★ 1.4k • written 3.5 years ago by
theemperor79
• 0
5
votes
6
replies
4.2k
views
Large difference in PCA plot based on LCPM (log counts per million) and VST (variance stabilizing transformations)
PCAtools
edgeR
pcaExplorer
RNASeq
RNAseq123
3.5 years ago
Alan
• 0
0
votes
0
replies
1.2k
views
Only 1 gene/ feature is present in module after WGCNA analysis
RNASeq
Clustering
cluster
WGCNA
3.5 years ago
chiwut.wong
• 0
0
votes
0
replies
1.1k
views
Pathway Analysis using GAGE
gage
RNASeq
gageData
Pathways
3.5 years ago
OST
• 0
6
votes
4
replies
4.3k
views
confused with tximport counts abundance using salmon input
RNASeq
tximport
3.5 years ago
Al90
▴ 10
0
votes
7
replies
2.0k
views
DESeq2 on allelic reads
RNASeq
DifferentialExpression
DESeq2
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
Nayrouz
• 0
0
votes
1
reply
1.0k
views
Rtracklayer
rtracklayer
RNASeq
gff
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
edusbioinfo
• 0
0
votes
1
reply
1.2k
views
Differential Expression Analysis-Input is DESeq Standardized
rnaseq
RNASeqData
limma
limmaGUI
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
sushimoto
• 0
0
votes
3
replies
1.3k
views
DEseq2 Analysis over multiple tissues and conditions only gives 16 coefficients
DESeq2
RNASeq
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
niko.fleischer
• 0
0
votes
2
replies
2.9k
views
DESeq2 counts distribution across both genes and samples
deseq2
rnaseq
updated 3.5 years ago by
BarryGutierrez
• 0 • written 9.1 years ago by
igor
▴ 50
0
votes
0
replies
1.0k
views
How to perform transcription factor enrichment analysis (TFEA) for RNA-seq counts data.
TFEA.ChIP
RNASeq
RNASeqData
3.5 years ago
treebig44
▴ 40
1
vote
0
replies
854
views
Is that possible to find if the sequence is enriched by specific motifs
MotifDiscovery
RNASeq
3.5 years ago
Liliian
• 0
0
votes
0
replies
4.2k
views
Create a Gene Enrichment GO plot from goana output
goana
RNASeq
edgeR
RnaSeq
GO
3.5 years ago
Raito92
▴ 60
1,409 results • Page
5 of 29
Recent ...
Replies
Comment: Help with defining groups
by
Phinney, Brett
▴ 10
I got it working , thank you!!!
Comment: Check removeBatchEffect effectiveness
by
massimo
• 0
I would kindly ask for additional support. When not including the design_timepoint argument in removeBatchEffect and thus keeping the inter…
Comment: Error Loading Experiment from SimBenchData Package
by
ksankaran
• 0
Aha, that was it! The object was class Seurat, but I didn't have it installed. After installing, everything works as expected.
Comment: Streamlining the computing time for MiloDE p-value correction in large dataset?
by
ATpoint
★ 5.0k
Is a method that is so computationally expensive really beneficial and necessary? You seem to have biological replication, don't you, so ps…
Answer: NA values for DESeq2 p-values and adjusted p-values using large sample sizes
by
ATpoint
★ 5.0k
Outliers without replacement, independent filtering or all-zero counts for that gene. That's the three options that trigger NAs https://bio…
Votes
Comment: Check removeBatchEffect effectiveness
Gene expression associated with continuous (quantitative) traits (Limma/edgeR/correlation)
Answer: Error Loading Experiment from SimBenchData Package
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
Awards
• All
Popular Question
to
Peter
• 0
Popular Question
to
Travis
• 0
Popular Question
to
James W. MacDonald
68k
Popular Question
to
Gordon Smyth
53k
Popular Question
to
shepherl
4.2k
Locations
• All
Seattle, WA, United States,
59 minutes ago
United States,
1 hour ago
UK/Cambridge,
1 hour ago
San Diego,
1 hour ago
Denmark,
1 hour ago
Traffic: 768 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6