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RnaSeq
•
reset
0
votes
2
replies
244
views
Standardization of rlog transformed counts before PCA
RNASeq
DESeq2
Visualization
updated 9 days ago by
ATpoint
★ 4.6k • written 14 days ago by
bbao
• 0
1
vote
5
replies
458
views
Deseq2 Design
RNASeq
DESeq2
updated 10 days ago by
James W. MacDonald
67k • written 14 days ago by
pm_25
• 0
0
votes
2
replies
436
views
sva: get a sv for RIN scores
limma
sva
variancePartition
voom
RNASeq
updated 19 days ago by
Gordon Smyth
52k • written 20 days ago by
Lina
▴ 10
0
votes
1
reply
156
views
WGCNA with TPM tables
RNASeq
WGCNA
ComparativeGenomics
updated 4 weeks ago by
ATpoint
★ 4.6k • written 4 weeks ago by
siobhan.dietz
• 0
0
votes
4
replies
316
views
Identifying potentially associated gene set to drug response using GSVA
drug
RNASeq
GSVA
updated 4 weeks ago by
Robert Castelo
★ 3.4k • written 5 weeks ago by
Kent
• 0
0
votes
1
reply
146
views
eRNAs count
RNASeq
DESeq2
updated 5 weeks ago by
ATpoint
★ 4.6k • written 5 weeks ago by
Irene
• 0
1
vote
8
replies
1.1k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 7 weeks ago by
Adam Marstrand
• 0
0
votes
0
replies
148
views
Combining two closely related bacterial annotated genomes to analyze as one pooled group
genomes
RNASeq
7 weeks ago
brayas13
• 0
3
votes
11
replies
770
views
Highly DE genes but small count difference within conditions
RNASeq
DESeq2
RNASeqData
written 11 weeks ago by
bioshot.com
• 0
2
votes
4
replies
444
views
RNASeq Time Course Question
DESeq2
RNASeq
TimeCourse
11 weeks ago
jihoon.kim.1495
▴ 10
1
vote
1
reply
320
views
Deseq2 sfType
Microbiome
DESeq2
RNASeq
updated 12 weeks ago by
James W. MacDonald
67k • written 12 weeks ago by
Caroline
• 0
1
vote
2
replies
480
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 12 weeks ago by
Michael Love
43k • written 3 months ago by
Ben
▴ 10
0
votes
3
replies
421
views
Meta-analysis on RNA-seq data sets
meta-analysis
sva
Combat_Seq
RNAseq
3 months ago
Travis
• 0
0
votes
5
replies
1.7k
views
DESeq2 Multi-factor design not full rank
RNASeq
DESeq2
written 3.6 years ago by
jehimi
• 0
0
votes
2
replies
918
views
Understanding Contrasts and Interaction in DESeq2 for 2 genotypes, 2 treatments
RNASeq
DESeq2
updated 3 months ago by
swbarnes2
★ 1.4k • written 3 months ago by
Shwetal
• 0
3
votes
5
replies
1.0k
views
DESeq2: How to Compare the Condition Effects of Two Different Treatments?
DESeq2
RNAseq
DifferentialExpression
updated 4 months ago by
swbarnes2
★ 1.4k • written 4 months ago by
BioNovice247
• 0
2
votes
3
replies
561
views
RUV - Does my data need further normalisation?
EDASeq
plotRLE
RNAseq
4 months ago
BioinfGuru
▴ 70
3
votes
6
replies
825
views
A question occur when using DESeq2 on a complex design
DESeq2
rnaseqGene
RNASeq
written 5 months ago by
MedocRafael
• 0
4
votes
3
replies
1.2k
views
DESeq fitNbinomGLMs Warning Issue: In fitNbinomGLMs(objectNZ[fitidx, , drop = FALSE], alpha_hat = alpha_hat[fitidx], : 4189rows had non-positive e…
DESeq2
RNAseq
written 5 months ago by
mila
▴ 10
0
votes
3
replies
593
views
Error when trying to do different pairwise comparisons and lfcshrink than what's listed on resultsNames()
RNASeqData
DESeq2
RNASeq
DifferentialExpression
updated 5 months ago by
ATpoint
★ 4.6k • written 5 months ago by
Mark
• 0
1
vote
4
replies
1.5k
views
Should I perform batch correction of normalized RNA-seq counts prior to GSVA?
GSVA
GSVAdata
RNAseq
5 months ago
Lucy
▴ 60
0
votes
4
replies
603
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
written 5 months ago by
Shaozhen
• 0
0
votes
0
replies
352
views
Cell type deconvolution of RNAseq data- Create signature matrix of cells
deconvolution
rnaseqGene
RNAseq
6 months ago
JG13
• 0
0
votes
1
reply
385
views
Significant genes in DESeq2 analysis
DESeq2
RNASeq
updated 6 months ago by
James W. MacDonald
67k • written 6 months ago by
cbio
• 0
1
vote
9
replies
1.5k
views
Most enriched pathway is not expressed when modifying universe parameter
DESeq2
clusterProfiler
Pathways
RNASeq
org.Mm.eg.db
updated 6 months ago by
James W. MacDonald
67k • written 6 months ago by
adrip
▴ 10
1
vote
3
replies
584
views
Including infectivity information to RNA-Seq analysis
RNASeqData
DESeq2
RNA-Seq
RNASeq
updated 6 months ago by
James W. MacDonald
67k • written 6 months ago by
Ben
▴ 50
2
votes
2
replies
649
views
How to remove batch effect to combine RNAseq data from 2 different experiments?
BatchQC
BatchEffect
RNASeq
updated 6 months ago by
jessica.mcclintock
▴ 10 • written 7 months ago by
ttt
• 0
0
votes
6
replies
1.3k
views
Using featureCounts with SAF annotation file gives "Error: only GTF files contain additional attributes"
RNASeq
Rsubread
7 months ago
atan
• 0
3
votes
2
replies
688
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
7 months ago
mohammedtoufiq91
▴ 10
0
votes
0
replies
310
views
scRNA-seq data trained model can be used for predictions on bulk RNA-seq data?
RNASeq
scRNAseq
7 months ago
Bibi
• 0
10
votes
11
replies
2.4k
views
SGSeq: moving toward diffex from SGSeq analysis
sgseq
rnaseq
updated 7 months ago by
Sara
• 0 • written 7.3 years ago by
Sylvain Foisy
▴ 80
1
vote
3
replies
667
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 7 months ago by
Gordon Smyth
52k • written 7 months ago by
Ashley
• 0
0
votes
3
replies
821
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 7 months ago by
ATpoint
★ 4.6k • written 7 months ago by
james.zhang20
• 0
0
votes
1
reply
525
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 8 months ago by
Michael Love
43k • written 8 months ago by
kcarey
• 0
0
votes
2
replies
462
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 8 months ago by
taur.vil
• 0
0
votes
2
replies
612
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
8 months ago
catv
• 0
5
votes
3
replies
934
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 7 months ago by
Gordon Smyth
52k • written 8 months ago by
Shaimaa Gamal
• 0
0
votes
1
reply
622
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 8 months ago by
Michael Love
43k • written 8 months ago by
09191919
• 0
0
votes
1
reply
549
views
Can you find the correct transcript in scRNA seq data
scRNAseq
RNAseq
updated 8 months ago by
ATpoint
★ 4.6k • written 8 months ago by
Merav
• 0
0
votes
2
replies
1.1k
views
Filtering and Reference Level for DESeq2
DESeq2
GeneFiltering
RNAseq
9 months ago
kcarey
• 0
2
votes
2
replies
539
views
Question about extracting co-efficients for pairwise comparisons in edgeR
limma
fit
design
RNASeq
edgeR
9 months ago
Sabiha
▴ 20
0
votes
2
replies
1.4k
views
RNA sequencing raw counts gene filtering
DESeq2
limma
RNAseq
9 months ago
kcarey
• 0
1
vote
4
replies
572
views
DESeq2 Replicates
DESeq2
RNASeq
9 months ago
kcarey
• 0
1
vote
3
replies
706
views
DESEQ2 Batch Effect
DESeq2
BatchEffect
RNASeq
updated 9 months ago by
swbarnes2
★ 1.4k • written 10 months ago by
sap275
▴ 10
0
votes
4
replies
685
views
Interactions in batches of varying size with different number of replicates using limma
limma
RNASeq
DifferentialExpression
10 months ago
mperalc
• 0
0
votes
2
replies
791
views
Error in featureCounts matrix
GenomeWideAssociation
countsimQC
RNASeq
updated 10 months ago by
Gordon Smyth
52k • written 10 months ago by
ryann
• 0
3
votes
5
replies
1.2k
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 9 months ago by
Michael Love
43k • written 10 months ago by
sap275
▴ 10
2
votes
1
reply
506
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 10 months ago by
Michael Love
43k • written 10 months ago by
sap275
▴ 10
3
votes
6
replies
1.2k
views
Average biological replicates in RNA-seq
Mfuzz
RNASeq
10 months ago
boczniak767
▴ 740
0
votes
0
replies
518
views
Differential expression analysis between different species
DEG
RNASeqData
DifferentialExpression
SCBN
RNASeq
10 months ago
Abril
• 0
50 results • Page
1 of 1
Recent ...
Replies
Comment: ChAMP.DMR error - Error in makeGenomicRatioSetFromMatrix
by
O'Brien
• 0
Consult the ChAMP.DMR documentation for more information on supported array types and expected data formats: https://bioconductor.org/packa…
Answer: combining raw counts and estimated counts
by
O'Brien
• 0
It is possible to combine raw counts from TCGA and estimated counts from GEO datasets (Kalisto pseudoalignment) while mitigating batch effe…
Comment: Packages don't update, but no error or warning is thrown
by
neighsanlo
• 0
This is useful information.
Comment: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
by
freemanmclaughlin9969289
• 0
Tools such as Pathway Analysis with Deweighting of Overlapping Genes ([block blast][1]) or Gene Set Enrichment Analysis for Single Cell RNA…
Comment: How to get around the warning message: mzR has been built against a different Rc
by
Nan
• 0
Hello, I was wondering if you were able to resolve this issue? I've recently encountered a similar problem and would like to ask for your a…
Votes
A: RNA-seq Normalisation - normalise all samples in experiment or only the ones use
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
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