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DESEq2
•
reset
9
votes
12
replies
7.8k
views
Multi factor design edgeR/Deseq2
deseq2
edgeR
rna-seq
updated 24 days ago by
Elizabeth
• 0 • written 8.8 years ago by
gthm
▴ 30
6
votes
19
replies
2.5k
views
DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
deseq2
updated 12 days ago by
Sey R.
• 0 • written 4.6 years ago by
hsbio
▴ 10
0
votes
2
replies
1.5k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 3 days ago by
maria.soler
• 0 • written 15 months ago by
kalavattam
▴ 10
2
votes
5
replies
1.2k
views
DESeq2 remove batch effect
BatchEffect
RNASeqData
WGCNA
DESeq2
updated 26 days ago by
Ashley
• 0 • written 11 months ago by
zxc741xb
• 0
2
votes
5
replies
776
views
DESeq2 setting significant p-value
DESeq2
updated 23 days ago by
Michael Love
42k • written 5.0 years ago by
Merlin
▴ 10
1
vote
2
replies
725
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
25 days ago • updated 16 days ago
ussarizona
▴ 10
0
votes
4
replies
613
views
How to correct for known and surrogate variables in DESeq2?
DESeq2
BatchEffect
sva
limma
updated 26 days ago by
Michael Love
42k • written 6 months ago by
Crystal571
• 0
1
vote
4
replies
499
views
DEseq2 coefficient
DESeq2
coefficient
11 weeks ago • updated 11 days ago
JKim
• 0
0
votes
7
replies
436
views
DESeq filtering specific to contrasts
DESeq2
updated 23 days ago by
Michael Love
42k • written 12 weeks ago by
Emma
• 0
2
votes
9
replies
408
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 17 days ago by
James W. MacDonald
65k • written 18 days ago by
Alexandre
• 0
0
votes
8
replies
365
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 18 days ago by
swbarnes2
★ 1.4k • written 19 days ago by
Aaliya
• 0
1
vote
4
replies
345
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
19 days ago • updated 17 days ago
winwater0928
• 0
3
votes
1
reply
319
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 19 days ago by
Michael Love
42k • written 19 days ago by
i.sudbery
▴ 40
2
votes
7
replies
289
views
deseq2 results
DESeq2
DifferentialExpression
rnaseqGene
DifferentialRegulation
5 days ago • updated 2 days ago
sajadahmad41454
• 0
0
votes
3
replies
281
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 11 days ago by
Michael Love
42k • written 12 days ago by
HAK
• 0
0
votes
4
replies
280
views
DEG with low counts
DESeq2
updated 24 days ago by
Michael Love
42k • written 28 days ago by
Rajat
• 0
1
vote
3
replies
276
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 5 days ago by
ATpoint
★ 4.1k • written 11 days ago by
angelathuynh5
• 0
3
votes
3
replies
255
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 14 days ago by
ATpoint
★ 4.1k • written 14 days ago by
bioinf
▴ 10
0
votes
6
replies
255
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 9 days ago by
swbarnes2
★ 1.4k • written 10 days ago by
arfranco
▴ 130
1
vote
2
replies
250
views
How to retrieve detailed regression results from deseq2
DESeq2
4 weeks ago • updated 23 days ago
Weiqian
▴ 10
0
votes
4
replies
248
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
25 days ago • updated 23 days ago
lonn
• 0
1
vote
3
replies
239
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 17 days ago by
Michael Love
42k • written 17 days ago by
JalapenoCornbread
• 0
3
votes
3
replies
235
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 20 days ago by
Michael Love
42k • written 20 days ago by
WardDeb
• 0
1
vote
3
replies
226
views
Different Shrunk Log2FC according with reference
DESeq2
updated 19 days ago by
Michael Love
42k • written 19 days ago by
andrebolerbarros
▴ 20
2
votes
3
replies
220
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 8 days ago by
ATpoint
★ 4.1k • written 9 days ago by
Reza
• 0
0
votes
2
replies
220
views
PCA plot suggestions
DESeq2
PCAtools
updated 16 days ago by
Michael Love
42k • written 18 days ago by
Aaliya
• 0
0
votes
3
replies
213
views
Interaction Terms in DESeq2 - Shrinkage
DESeq2
updated 27 days ago by
Michael Love
42k • written 27 days ago by
andrebolerbarros
▴ 20
0
votes
3
replies
192
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 11 days ago by
ATpoint
★ 4.1k • written 11 days ago by
james.zhang20
• 0
0
votes
2
replies
185
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
11 days ago • updated 10 days ago
kcarey
• 0
0
votes
1
reply
162
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 18 days ago by
Michael Love
42k • written 18 days ago by
kcarey
• 0
0
votes
1
reply
157
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
updated 25 days ago by
ATpoint
★ 4.1k • written 26 days ago by
quanah.hudson
• 0
1
vote
1
reply
154
views
Handling multiple differential expression comparisons
DESeq2
updated 8 days ago by
Michael Love
42k • written 9 days ago by
Zainab
▴ 10
0
votes
2
replies
149
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
13 days ago • updated 10 days ago
Aurora
• 0
0
votes
1
reply
147
views
rlog transformation
DESeq2
updated 24 days ago by
Michael Love
42k • written 25 days ago by
ishij9717
• 0
1
vote
1
reply
140
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
updated 13 days ago by
Michael Love
42k • written 13 days ago by
marcusn
• 0
0
votes
2
replies
139
views
DESeq2 v1.35.0
DESeq2
updated 10 days ago by
Michael Love
42k • written 10 days ago by
Espresso
• 0
0
votes
1
reply
139
views
covariate with negative and positive values
DESeq2
updated 24 days ago by
Michael Love
42k • written 26 days ago by
Brynn
• 0
0
votes
1
reply
138
views
Deseq2 Rlog transformation
DESeq2
updated 28 days ago by
ATpoint
★ 4.1k • written 29 days ago by
Sahar
• 0
0
votes
1
reply
125
views
putting my own expression data in deseq2
DESeq2
updated 13 days ago by
ATpoint
★ 4.1k • written 13 days ago by
Dev
• 0
0
votes
1
reply
125
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 13 days ago by
Michael Love
42k • written 13 days ago by
Agung
• 0
0
votes
1
reply
119
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 24 days ago by
Michael Love
42k • written 25 days ago by
09191919
• 0
0
votes
1
reply
81
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 3 days ago by
ATpoint
★ 4.1k • written 3 days ago by
sajadahmad41454
• 0
0
votes
1
reply
72
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 4 days ago by
ATpoint
★ 4.1k • written 4 days ago by
sajadahmad41454
• 0
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Comment: ChAMP installation issue
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Without showing the commands you tried and the full output including error it is impossible to help. Please provide additional information…
Answer: Gene set enrichment analysis
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No, that is not possible. The idea of gene set enrichment analysis (GSEA) is that it checks which gene sets (pathways, GO categories) are e…
Comment: error in limma , contrast.matrix Number of rows of contrast matrix must match nu
by
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• 0
thank you for your response. The problem is resolved. When i did a fit for the linear model i put NULL for the design fit <- lmFit(count_…
Comment: More regions in union when increasing DBA$config$mergeOverlap
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I am so confused and frustrated learning to use this software. Isn't how you merge the peaksets one of the most important steps for the com…
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Votes
A: DEXSeq time course design
using variable in design formula
differential expression for Single-cell RNAseq data using edgeR
Why p value inconsistent when using different number of pathway score?
Answer: error in limma , contrast.matrix Number of rows of contrast matrix must match nu
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