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DESeq2
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0
votes
0
replies
6
views
How to add Ensembl ids after Pseudobulk analysis by DESeq2
Seurat
DESeq2
single-cell
scRNA
Pseudobulk
1 hour ago
Sara
• 0
0
votes
1
reply
51
views
GSVA function
DESeq2
GSVA
ssGSEA
updated 1 day ago by
Robert Castelo
★ 3.3k • written 1 day ago by
michelafrancesconi8
• 0
0
votes
1
reply
55
views
Normalisation using clinical traits in RNA seq using DESeq2
DESeq2
clinicaltraits
Normalization
batchnormalisation
updated 1 hour ago by
ATpoint
★ 4.2k • written 1 day ago by
sukeshinik5
• 0
0
votes
4
replies
149
views
Volcano plot of scRNA-seq data not "centered"
DESeq2
updated 1 hour ago by
ATpoint
★ 4.2k • written 2 days ago by
tjl
• 0
1
vote
8
replies
163
views
Slightly different count plots from R and spreadsheet
DESeq2
ggplot2
3 days ago
boczniak767
▴ 720
1
vote
3
replies
155
views
LRT, more than two conditions and pairwise comparisons
DESeq2
updated 5 days ago by
James W. MacDonald
66k • written 6 days ago by
boczniak767
▴ 720
0
votes
6
replies
616
views
Numerical differences in DESeq2 output depending on different design matrix construction
DESeq2
9 days ago • updated 5 days ago
Ken C
• 0
2
votes
4
replies
228
views
DESeq2- Mysterious interaction suppresses DEGs
DESeq2
PCAplot
RNA-Seq
DesignModel
updated 5 days ago by
Michael Love
42k • written 8 days ago by
shaunpeterson
• 0
4
votes
9
replies
330
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 9 days ago by
ATpoint
★ 4.2k • written 11 days ago by
pangtaihin
• 0
0
votes
3
replies
163
views
Assymetric MA plot with no overall trend
DESeq2
DifferentialExpression
updated 10 days ago by
Michael Love
42k • written 11 days ago by
Kasper
• 0
0
votes
1
reply
124
views
normalization across different species for DESeq2 analysis
DESeq2
normalization
Normalization
acrossspecies
updated 11 days ago by
Michael Love
42k • written 12 days ago by
piemmea
• 0
0
votes
8
replies
573
views
Can I trust the differential expression analysis when the two groups overlap on the PCA?
DESeq2
pcaExplorer
updated 11 days ago by
BioinfGuru
▴ 30 • written 12 days ago by
Eric Katagirya
• 0
1
vote
2
replies
277
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
DESeq2
eigencorplot
PCAtools
12 days ago • updated 9 days ago
BioinfGuru
▴ 30
0
votes
1
reply
201
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 16 days ago by
ATpoint
★ 4.2k • written 16 days ago by
sajadahmad41454
• 0
0
votes
1
reply
123
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 17 days ago by
ATpoint
★ 4.2k • written 17 days ago by
sajadahmad41454
• 0
2
votes
7
replies
466
views
deseq2 results
DESeq2
DifferentialExpression
rnaseqGene
DifferentialRegulation
18 days ago • updated 14 days ago
sajadahmad41454
• 0
1
vote
1
reply
199
views
Handling multiple differential expression comparisons
DESeq2
updated 20 days ago by
Michael Love
42k • written 22 days ago by
Zainab
▴ 10
0
votes
6
replies
320
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 22 days ago by
swbarnes2
★ 1.4k • written 23 days ago by
arfranco
▴ 130
2
votes
3
replies
276
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 21 days ago by
ATpoint
★ 4.2k • written 22 days ago by
Reza
• 0
0
votes
2
replies
187
views
DESeq2 v1.35.0
DESeq2
updated 23 days ago by
Michael Love
42k • written 23 days ago by
Espresso
• 0
0
votes
2
replies
222
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
24 days ago • updated 23 days ago
kcarey
• 0
0
votes
3
replies
245
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 23 days ago by
ATpoint
★ 4.2k • written 24 days ago by
james.zhang20
• 0
1
vote
3
replies
325
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 18 days ago by
ATpoint
★ 4.2k • written 24 days ago by
angelathuynh5
• 0
0
votes
3
replies
310
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 24 days ago by
Michael Love
42k • written 25 days ago by
HAK
• 0
1
vote
1
reply
164
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
updated 25 days ago by
Michael Love
42k • written 25 days ago by
marcusn
• 0
0
votes
2
replies
181
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
26 days ago • updated 23 days ago
Aurora
• 0
0
votes
1
reply
144
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 25 days ago by
Michael Love
42k • written 26 days ago by
Agung
• 0
0
votes
1
reply
143
views
putting my own expression data in deseq2
DESeq2
updated 25 days ago by
ATpoint
★ 4.2k • written 26 days ago by
Dev
• 0
3
votes
3
replies
281
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 27 days ago by
ATpoint
★ 4.2k • written 27 days ago by
bioinf
▴ 10
0
votes
2
replies
239
views
PCA plot suggestions
DESeq2
PCAtools
updated 29 days ago by
Michael Love
42k • written 4 weeks ago by
Aaliya
• 0
1
vote
3
replies
262
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 29 days ago by
Michael Love
42k • written 4 weeks ago by
JalapenoCornbread
• 0
2
votes
9
replies
460
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 4 weeks ago by
James W. MacDonald
66k • written 4 weeks ago by
Alexandre
• 0
0
votes
1
reply
176
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
kcarey
• 0
1
vote
3
replies
249
views
Different Shrunk Log2FC according with reference
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
andrebolerbarros
▴ 20
1
vote
4
replies
374
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
4 weeks ago
winwater0928
• 0
3
votes
1
reply
342
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
i.sudbery
▴ 40
0
votes
8
replies
423
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 4 weeks ago by
swbarnes2
★ 1.4k • written 4 weeks ago by
Aaliya
• 0
3
votes
3
replies
268
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
WardDeb
• 0
0
votes
1
reply
162
views
rlog transformation
DESeq2
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
ishij9717
• 0
0
votes
1
reply
137
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
09191919
• 0
1
vote
2
replies
779
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
5 weeks ago • updated 29 days ago
Patadù
▴ 10
0
votes
1
reply
154
views
covariate with negative and positive values
DESeq2
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
Brynn
• 0
0
votes
1
reply
172
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
updated 5 weeks ago by
ATpoint
★ 4.2k • written 5 weeks ago by
quanah.hudson
• 0
0
votes
4
replies
279
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
5 weeks ago
lonn
• 0
0
votes
3
replies
237
views
Interaction Terms in DESeq2 - Shrinkage
DESeq2
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
andrebolerbarros
▴ 20
1
vote
2
replies
277
views
How to retrieve detailed regression results from deseq2
DESeq2
6 weeks ago • updated 5 weeks ago
Weiqian
▴ 10
0
votes
4
replies
303
views
DEG with low counts
DESeq2
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
Rajat
• 0
0
votes
1
reply
158
views
Deseq2 Rlog transformation
DESeq2
updated 5 weeks ago by
ATpoint
★ 4.2k • written 6 weeks ago by
Sahar
• 0
1
vote
2
replies
218
views
Design Matrix within group comparison more than two groups
DESeq2
6 weeks ago
hemantcnaik
• 0
0
votes
1
reply
156
views
Bioinformatics student
Des
DESeq2
updated 6 weeks ago by
hemantcnaik
• 0 • written 6 weeks ago by
Sahar
• 0
50 results • Page
1 of 1
Recent ...
Replies
Comment: Volcano plot of scRNA-seq data not "centered"
by
ATpoint
★ 4.2k
My comment was generic. If your experiment causes drastic shifts then the situation might be different. The advise is the same: Try to use …
Comment: conumee with Infinium MethylationEPIC v2.0
by
Maximus
• 0
Hi Sole, If there is a combined mixture of MSet of 450K, EPIC and EPICv2, how should I create an annotation object? I tried to create anno…
Comment: Volcano plot of scRNA-seq data not "centered"
by
tjl
• 0
Thank you both. The code is below, simplified a bit for simplicity. ATpoint, your suggestion re: the top 20% of genes by baseMean makes me …
Comment: Cannot install oligo on Fedora
by
James W. MacDonald
66k
Wait a minute. The `oligo` package is *supposed to* be using `zlibbioc`, but apparently it is not. I get the same thing as you do, where it…
Comment: Cannot install oligo on Fedora
by
James W. MacDonald
66k
There is the `zlibbioc` package that provides the headers for zlib 1.2.5, that I think you should be able to use, although that would requi…
Votes
DeSeq2 results converting ENSG IDs to Gene Symbols, more than one ENSG ID per Gene symbol
DESeq2 swap ensembl gene id rownames with gene symbol column
A: DeSeq2 results converting ENSG IDs to Gene Symbols, more than one ENSG ID per Ge
DESeq2: add annotations (from data frame) to DESeqDataSet
Add new columns to DESeqResults from text file by matching rownames.
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