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Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
Michael Love
41k
With 3 vs 3, when one sample increases from 50 to 5000, this can lead to rejection of null hypothesis. I'm not surprised with the above cou…
Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
samer
•
0
This is the result i got when i only looked on day 04 for the gene in example 1 posted above
dds_sub <- dds[, dds$date %in% c("day04"…
Answer: What is the meaning of "CpG interrogation" and "single nucleotide extension(SBE)
by
James W. MacDonald
64k
There are two types of probes used by Illumina. The type I probes bind directly to the CpG site and have two different probes (one for a me…
Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
Michael Love
41k
Then for the remaining genes that you don't think are DE, just plot like this:
```
dds_sub <- dds[,dds$condition %in% c("x","y")]
dropleve…
Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
samer
•
0
yes i turned it off @mikelove
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