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rnaseq
•
reset
0
votes
4
replies
257
views
Using featureCounts with SAF annotation file gives "Error: only GTF files contain additional attributes"
RNASeq
Rsubread
updated 3 days ago by
Wei Shi
★ 3.6k • written 4 days ago by
atan
• 0
2
votes
2
replies
147
views
Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
NanoStringNCTools
limma
ExpressionData
RNASeq
edgeR
6 days ago • updated 5 days ago
mohammedtoufiq91
▴ 10
1
vote
1
reply
200
views
How to remove batch effect to combine RNAseq data from 2 different experiments?
BatchQC
BatchEffect
RNASeq
updated 7 days ago by
James W. MacDonald
66k • written 7 days ago by
ttt
• 0
0
votes
0
replies
60
views
scRNA-seq data trained model can be used for predictions on bulk RNA-seq data?
RNASeq
scRNAseq
8 days ago
Bibi
• 0
1
vote
3
replies
291
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 13 days ago by
Gordon Smyth
50k • written 14 days ago by
Ashley
• 0
0
votes
3
replies
248
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 25 days ago by
ATpoint
★ 4.2k • written 26 days ago by
james.zhang20
• 0
0
votes
1
reply
177
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
kcarey
• 0
0
votes
2
replies
202
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 5 weeks ago by
taur.vil
• 0
0
votes
2
replies
257
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
5 weeks ago
catv
• 0
0
votes
1
reply
141
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
09191919
• 0
5
votes
3
replies
333
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 17 days ago by
Gordon Smyth
50k • written 5 weeks ago by
Shaimaa Gamal
• 0
0
votes
1
reply
161
views
Can you find the correct transcript in scRNA seq data
scRNAseq
RNAseq
updated 6 weeks ago by
ATpoint
★ 4.2k • written 7 weeks ago by
Merav
• 0
0
votes
2
replies
292
views
Filtering and Reference Level for DESeq2
DESeq2
GeneFiltering
RNAseq
9 weeks ago
kcarey
• 0
2
votes
2
replies
295
views
Question about extracting co-efficients for pairwise comparisons in edgeR
limma
fit
design
RNASeq
edgeR
10 weeks ago
Sabiha
▴ 20
0
votes
2
replies
339
views
RNA sequencing raw counts gene filtering
DESeq2
limma
RNAseq
10 weeks ago
kcarey
• 0
1
vote
4
replies
344
views
DESeq2 Replicates
DESeq2
RNASeq
11 weeks ago
kcarey
• 0
1
vote
3
replies
400
views
DESEQ2 Batch Effect
DESeq2
BatchEffect
RNASeq
updated 12 weeks ago by
swbarnes2
★ 1.4k • written 12 weeks ago by
sap275
▴ 10
0
votes
4
replies
342
views
Interactions in batches of varying size with different number of replicates using limma
limma
RNASeq
DifferentialExpression
3 months ago • updated 12 weeks ago
mperalc
• 0
0
votes
2
replies
363
views
Error in featureCounts matrix
GenomeWideAssociation
countsimQC
RNASeq
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
ryann
• 0
2
votes
1
reply
282
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 3 months ago by
Michael Love
42k • written 3 months ago by
sap275
▴ 10
3
votes
5
replies
680
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 12 weeks ago by
Michael Love
42k • written 3 months ago by
sap275
▴ 10
3
votes
6
replies
615
views
Average biological replicates in RNA-seq
Mfuzz
RNASeq
3 months ago
boczniak767
▴ 720
0
votes
0
replies
252
views
Differential expression analysis between different species
DEG
RNASeqData
DifferentialExpression
SCBN
RNASeq
3 months ago
Abril
• 0
2
votes
2
replies
453
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
3 months ago
jackafiore
• 0
0
votes
4
replies
451
views
Ballgown error in scan for c.tab files
ballgown
RNASeq
3 months ago
candacenewcome
• 0
1
vote
3
replies
369
views
DESeq2 analysis on time-series RNA experiment
RNASeqData
DESeq2
RNASeq
3 months ago
island
• 0
1
vote
3
replies
644
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics
Normalization
RNASeq
DESeq2
written 4 months ago by
georgii.vdovin
• 0
0
votes
1
reply
269
views
Error in GDCRNATools
ceRNA
GDCRNATools
RNASeq
updated 4 months ago by
Ram
▴ 210 • written 4 months ago by
Matvii Mykhailichenko
• 0
2
votes
4
replies
671
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
4 months ago
Sabiha
▴ 20
4
votes
6
replies
662
views
filtering lowly expressed reads
RNASeq
limma
DifferentialExpression
Preprocessing
updated 3 months ago by
Gordon Smyth
50k • written 4 months ago by
Shaimaa Gamal
• 0
3
votes
1
reply
342
views
Dataset imbalance
RNASeq
DifferentialExpression
RnaSeqSampleSizeData
RnaSeqSampleSize
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
Shaimaa Gamal
• 0
0
votes
3
replies
583
views
dittobarplot Var error
RNASeq
ditto
dittoSeq
SingleR
written 4 months ago by
Fayrouz
• 0
0
votes
1
reply
294
views
DESeq Design for Time-Series Analysis
RNASeq
DESeq2
timecourse
4 months ago
WS
• 0
1
vote
2
replies
510
views
How to perform longitudinal analysis using EdgeR
StatisticalMethod
FoldChange
edgeR
RNASeq
5 months ago
Sabiha
▴ 20
2
votes
3
replies
1.3k
views
Using STAR's readspergene.tab.out outputs to make gene-level count matrix for DESeq2 using tximport
STAR
tximport
RNASeq
DESeq2
updated 5 months ago by
swbarnes2
★ 1.4k • written 5 months ago by
Bo
• 0
1
vote
2
replies
478
views
RNASeq time course analysis, missing time points for control
RNASeqData
RnaSeqSampleSize
RNASeq
RNASeqPower
5 months ago
drewhaydensteele
• 0
7
votes
2
replies
898
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR
limma
voom
RNASeq
muscat
updated 5 months ago by
Gordon Smyth
50k • written 5 months ago by
annikagable
• 0
1
vote
1
reply
398
views
Using DESeq2 statistical framework with to identify differentially expressed loci instead of genes
rnaseq
DESeq2
updated 6 months ago by
Michael Love
42k • written 6 months ago by
pl23
• 0
2
votes
3
replies
540
views
DESeq2 estimateSizeFactors iterate takes too long
RNASeq
DESeq2
6 months ago
Karthik
• 0
0
votes
3
replies
499
views
'Subtracting' counts on basis of Negative control media and water samples
limma
edgeR
RNASeq
DESeq2
6 months ago • updated 5 months ago
Karthik
• 0
2
votes
2
replies
447
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
6 months ago
Manthos
• 0
2
votes
4
replies
655
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom
RNASeq
limma
6 months ago
Tyler Sagendorf
▴ 10
1
vote
4
replies
670
views
controling for batch effect while preserving variation within repeated measures that unevenly distributed across batches
miRNA
BatchEffect
RNASeq
limma
6 months ago
Wending
• 0
0
votes
0
replies
379
views
Include or exclude N/A genes (those without any KEGG annotation) when running Wilcox test?
KEGGREST
KEGG
RNASeq
6 months ago
mch
• 0
4
votes
2
replies
704
views
DecideTest vs topTAGs in edgeR
RNAseq
StatisticalMethod
edgeR
updated 7 months ago by
Gordon Smyth
50k • written 7 months ago by
alvaroperona1
▴ 10
0
votes
6
replies
938
views
DESeq2 Normalization with 4 Groups
Normalization
RNASeq
DESeq2
7 months ago
erika.maldonado-rosado
• 0
0
votes
2
replies
570
views
TCGA counts to TPM from recount3 do not seem to right
recount3
TCGA
RNASeq
updated 6 months ago by
Leonardo Collado Torres
★ 1.1k • written 7 months ago by
tangming2005
▴ 190
0
votes
1
reply
464
views
DESeq2 model for two genome system
RNASeq
DESeq2
updated 6 months ago by
Michael Love
42k • written 7 months ago by
Sean
• 0
0
votes
0
replies
358
views
is Dada2 biased toward a particular sequence ?
virology
dada2
amplican
Sequencing
RNASeq
7 months ago
Sara
• 0
0
votes
2
replies
667
views
Will DESeq2 be appropriate tool for analysis?
RNASeq
DESeq2
7 months ago
Karthik
• 0
50 results • Page
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Recent ...
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Comment: Why is longestConsecutive() depreceated in version 2.72 (BioC version 3.19)?
by
ATpoint
★ 4.2k
I don't have an answer to the "why" but maybe a simple reimplemention will do: ```r seqs <- c("ATCATCGTATCAAAGTC", "AAAACGTATCAAAGTC", "TG…
Comment: How to add Ensembl ids after Pseudobulk analysis by DESeq2
by
Guido Hooiveld
★ 4.0k
After checking `?DESeqDataSetFromMatrix` note this information: arguments provided to SummarizedExperiment including rowRanges and met…
Answer: Error in gseDO function
by
Guido Hooiveld
★ 4.0k
Yes, you are missing something. :-) For a GSEA you will need to use as input all genes that you are analyzing; these genes should be ran…
Comment: GSVA function
by
Robert Castelo
★ 3.3k
No, negative values may occur after log transformation when the `log(x)` is taken from a positive number `x with 0 < x < 1`, and therefore …
Comment: conumee with Infinium MethylationEPIC v2.0
by
sgomez
• 0
Hi Max, Could you try this? https://support.bioconductor.org/p/9136138/#9136840
Votes
A: Comparison between two different group's pathway analysis results
DiffBind Batch Effect
A: DiffBind Batch Effect
A: DiffBind Batch Effect
C: DiffBind Batch Effect
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★ 3.6k
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50k
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