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DESEQ2
•
reset
0
votes
0
replies
13
views
DESeq2 interactions
DESeq2
6 hours ago
Michael
• 0
3
votes
14
replies
728
views
DESeq2 used with zinbwave weights - Differences on contrasts calculations
zinbwave
DESeq2
written 6 weeks ago by
Aurélie
• 0
0
votes
2
replies
111
views
3-factor DESeq2 trime-series analysis
time-series
DESeq2
4 days ago • updated 3 days ago
gogeni5529
• 0
0
votes
3
replies
212
views
Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
RUVSeq
DESeq2
updated 3 days ago by
Michael Love
43k • written 12 days ago by
yongjie.wang
• 0
0
votes
1
reply
185
views
Which of apeglm and ashr may be more appropriate for pseudobulked DESeq2 analysis of single-cell RNA-seq data?
apeglm
DESeq2
updated 6 days ago by
Michael Love
43k • written 20 days ago by
KS
• 0
3
votes
5
replies
926
views
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
DESeq2
updated 12 days ago by
James W. MacDonald
68k • written 18 days ago by
snijesh
▴ 200
17
votes
45
replies
12k
views
Can NanoString data be analyzed using DESeq2?
NanoString
Differential Expressed Genes Analysis
DESeq2
updated 19 days ago by
James W. MacDonald
68k • written 6.1 years ago by
lim6432
▴ 60
0
votes
0
replies
84
views
DESeq2 on non-RNASeq counts data
DESeq2
25 days ago
vs
• 0
1
vote
9
replies
755
views
Using DESeq2 on single cell RNA Seq data of same cell type but different conditions that were pseudo bulked
DESeq2
scRNAseq
27 days ago • updated 23 days ago
Raya
• 0
0
votes
11
replies
414
views
Getting way too many DEGs with LRT for time-series RNA-seq!
TimeCourse
DESeq2
29 days ago • updated 24 days ago
Denise
• 0
2
votes
6
replies
371
views
Very different log2 Fold changes with DESeq2 and explict calculation
DESeq2
4 weeks ago
richardallenfriedmanbrooklyn
▴ 30
0
votes
3
replies
179
views
DESeq2 inconsistent fits depending on model design with interacting factors
des
DESeq2
written 4 weeks ago by
fedor.bezrukov
▴ 10
0
votes
4
replies
777
views
Using shrunken LFC and s-value from DESeq2 as DGE results
apeglm
s-value
DESeq2
updated 5 weeks ago by
Michael Love
43k • written 8 weeks ago by
Erika
• 0
1
vote
2
replies
326
views
Differential expression on the transcript-level
DESeq2
updated 6 weeks ago by
swbarnes2
★ 1.4k • written 7 weeks ago by
泓朴
• 0
3
votes
11
replies
1.8k
views
Highly DE genes but small count difference within conditions
RNASeq
DESeq2
RNASeqData
written 11 months ago by
bioshot.com
• 0
4
votes
8
replies
1.7k
views
Should Xenium pseudobulk experiments be analysed like microarrays (limma) or like RNASeq (edgeR,Deseq2, or limma-voom)
Deseq2
edgeR
limma
xenium
Voom
9 weeks ago • updated 8 weeks ago
richardallenfriedmanbrooklyn
▴ 30
7
votes
9
replies
5.3k
views
competitive enrichment test yields too few significant results, self-contained too many
limma
DESeq2
fry
edgeR
9 weeks ago • updated 8 weeks ago
bbao
▴ 10
0
votes
2
replies
895
views
Install old version of DESeq2
apeglm
Install
DESeq2
version
updated 9 weeks ago by
James W. MacDonald
68k • written 9 weeks ago by
Asma
• 0
0
votes
1
reply
427
views
discrepancy in IDs from file and tx2gene (IDs don't match)
tximport
DESeq2
updated 9 weeks ago by
James W. MacDonald
68k • written 9 weeks ago by
IUH
• 0
0
votes
2
replies
893
views
gene detection across experiments
DESeq2
Normalization
11 weeks ago • updated 10 weeks ago
Mara
• 0
5
votes
11
replies
3.0k
views
parameters of filterByExpr
edgeR
DESeq2
filterByExpr
written 4 months ago by
caroline.zanchi
▴ 10
1
vote
5
replies
3.7k
views
Pre-filtering, adjusting independent filtering, or lfcthreshold() on DESeq2? Low counts and dropouts produce interesting volcano plots.
DESeq2
updated 7 weeks ago by
Michael Love
43k • written 3 months ago by
Alexander
• 0
1
vote
1
reply
2.1k
views
DESeq2 LRT: Testing Effects of Treatment1, Treatment2, and Their Interaction in a Four-Factor Design
DESeq2
multifactorialdesign
interaction
InteractionSet
updated 12 weeks ago by
Michael Love
43k • written 12 weeks ago by
Integrative
• 0
0
votes
2
replies
1.1k
views
6 groups to compare DESeq2 (Wald vs LRT)
DESeq2
updated 12 weeks ago by
Michael Love
43k • written 12 weeks ago by
Rocio
• 0
3
votes
2
replies
1.3k
views
Biological replicates consideration in DESeq2 design formula.
DESeq2
RNASeqData
RNASeq
written 3 months ago by
Christian
▴ 20
1
vote
2
replies
2.5k
views
Deseq2 paired sample in the design is considered as "batch"?
DESeq2
written 3 months ago by
corranna98
• 0
3
votes
1
reply
3.7k
views
DESeq2 empirical bayes: variance of the prior for log fold changes (LFC)
DESeq2
updated 3 months ago by
Michael Love
43k • written 3 months ago by
Chenghao
▴ 10
0
votes
1
reply
440
views
DESeq2 results
result
DESeq2
subsetting
updated 3 months ago by
ATpoint
★ 4.9k • written 3 months ago by
rajdeepboral00
• 0
0
votes
1
reply
355
views
Unclear DEseq2 replaceOutliers error message
DESeq2
updated 3 months ago by
Michael Love
43k • written 3 months ago by
Pak
• 0
0
votes
1
reply
453
views
Difference in DESeq2 output and tidybulk DESeq2 method
DifferentialExpression
DESeq2
tidybulk
RNASeq
updated 3 months ago by
Michael Love
43k • written 4 months ago by
s.malik
• 0
3
votes
2
replies
2.0k
views
Deseq2 DE analysis of host-pathogen samples (model separately or jointly?)
DESeq2
4 months ago • updated 3 months ago
dudutchy
▴ 10
2
votes
3
replies
1.2k
views
Differential gene expression analysis on haplotype-resolved diploid assembly
edgeR
DESeq2
haplotype
limma
3 months ago
Paulito
• 0
4
votes
4
replies
1.9k
views
Adding gene names as row annotations to an RNAseq heatmap made with ComplexHeatmap
DESeq2
ComplexHeatmap
3 months ago
caroline.zanchi
▴ 10
0
votes
1
reply
361
views
DESeq2 from phyloseq error
DESeq2
phyloseq
3 months ago
asafpr
• 0
3
votes
1
reply
546
views
DESeq2 Apeglm Coefficient Reordering / Log2FoldChange Comparison Change
DESeq2
apeglm
updated 4 months ago by
James W. MacDonald
68k • written 4 months ago by
sk
• 0
1
vote
5
replies
1.3k
views
Heatmap of a transformed deseq object with pheatmap
DESeq2
heatmaps
updated 4 months ago by
James W. MacDonald
68k • written 4 months ago by
caroline.zanchi
▴ 10
8
votes
3
replies
3.0k
views
DESeq2 class error: superclass "ExpData" not defined in the environment of the object's class
DESeq2
updated 3 months ago by
Michael Love
43k • written 4 months ago by
Cassia
▴ 40
2
votes
4
replies
1.6k
views
Filtering after DESeq
DESeq2
IHW
written 4 months ago by
caroline.zanchi
▴ 10
0
votes
4
replies
1.1k
views
DESEQ2 - Correctly altering log2foldchange when switching comparison direction (HELP GREATLY APPRECIATED)
DESeq2
4 months ago
sk
• 0
0
votes
6
replies
1.7k
views
Discrepancies in normalised count data vs unnormalised
DESeq2
4 months ago • updated 3 months ago
AMgroup
• 0
0
votes
2
replies
628
views
Design formula for circadian time-course data for vst() function
DESeq2
4 months ago
Vishnu
• 0
0
votes
2
replies
765
views
Analyzing TnSeq data with DESeq2
DESeq2
written 4 months ago by
newfunland
• 0
0
votes
1
reply
1.1k
views
How do I set up my DESeq analysis when I know a few vehicle control samples provide background effects we don't want to see in any of the subsequent …
DESeq2
RUVSeq
updated 4 months ago by
Michael Love
43k • written 5 months ago by
Simran
• 0
0
votes
2
replies
654
views
Differing results with DESeq2
DESeq2
updated 4 months ago by
JKim
• 0 • written 5 months ago by
hampsdiv
• 0
0
votes
2
replies
539
views
RNA Seq using Lrt
DESeq2
4 months ago
Rob
• 0
0
votes
3
replies
937
views
Log2foldchange strange behavior
DESeq2
updated 5 months ago by
ATpoint
★ 4.9k • written 5 months ago by
RambakKulit
• 0
0
votes
1
reply
834
views
WGCNA
WGCNA
DESeq2
updated 5 months ago by
ATpoint
★ 4.9k • written 5 months ago by
bioinformaticssrm2011
• 0
0
votes
1
reply
1.0k
views
Comparison of results obtained from a subset of the data versus the complete dataset for a few selected genes.
DESeq2
updated 5 months ago by
swbarnes2
★ 1.4k • written 5 months ago by
Bioinformatician_R
▴ 20
1
vote
1
reply
1.3k
views
PhD scholar
RNASeqAnalysis
DESeq2
updated 5 months ago by
shepherl
4.2k • written 5 months ago by
Richa
• 0
0
votes
1
reply
447
views
DESeq2 - am I extracting results incorrectly?
DESeq2
updated 5 months ago by
James W. MacDonald
68k • written 5 months ago by
bd2000
▴ 30
4,835 results • Page
1 of 97
Recent ...
Replies
Comment: Heatmap of the Count Matrix
by
ArnulfoByrd
• 0
Perhaps PCA loadings offer a more comprehensive perspective. Now, thinking back, I recall struggling with a similar data reduction dilemma …
Answer: readBam vs read_bam_insert_metrics
by
James W. MacDonald
68k
Depends on what you mean by input. If you mean to ask if you use `readBam` to generate something to use as an argument to `read_bam_insert_…
Answer: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
James W. MacDonald
68k
There is an example in the help page for `forgeBSgenomePkgFromTwobitFile`. Have you tried emulating that?
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
by
Gordon Smyth
53k
What you describe sounds a good approach to me. I would also use sample weights (`sample.weights=TRUE` when running `voomLmFit`) and robust…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment". I don't know about `offst()` function, what is the scale or direction, or whether you a…
Votes
Answer: Limpa Pipeline Order
A: How barcode-plot enrichment is calculated?
A: How barcode-plot enrichment is calculated?
Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
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