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DESeq2
•
reset
1
vote
8
replies
132
views
Slightly different count plots from R and spreadsheet
DESeq2
ggplot2
20 hours ago • updated 12 hours ago
boczniak767
▴ 720
1
vote
3
replies
135
views
LRT, more than two conditions and pairwise comparisons
DESeq2
updated 2 days ago by
James W. MacDonald
66k • written 3 days ago by
boczniak767
▴ 720
0
votes
6
replies
594
views
Numerical differences in DESeq2 output depending on different design matrix construction
DESeq2
5 days ago • updated 2 days ago
Ken C
• 0
2
votes
4
replies
214
views
DESeq2- Mysterious interaction suppresses DEGs
DESeq2
PCAplot
RNA-Seq
DesignModel
updated 2 days ago by
Michael Love
42k • written 5 days ago by
shaunpeterson
• 0
4
votes
9
replies
307
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 6 days ago by
ATpoint
★ 4.1k • written 8 days ago by
pangtaihin
• 0
0
votes
3
replies
150
views
Assymetric MA plot with no overall trend
DESeq2
DifferentialExpression
updated 7 days ago by
Michael Love
42k • written 8 days ago by
Kasper
• 0
0
votes
1
reply
117
views
normalization across different species for DESeq2 analysis
DESeq2
normalization
Normalization
acrossspecies
updated 8 days ago by
Michael Love
42k • written 9 days ago by
piemmea
• 0
0
votes
8
replies
551
views
Can I trust the differential expression analysis when the two groups overlap on the PCA?
DESeq2
pcaExplorer
updated 8 days ago by
BioinfGuru
▴ 30 • written 9 days ago by
Eric Katagirya
• 0
1
vote
2
replies
269
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
DESeq2
eigencorplot
PCAtools
9 days ago • updated 6 days ago
BioinfGuru
▴ 30
0
votes
1
reply
192
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 13 days ago by
ATpoint
★ 4.1k • written 13 days ago by
sajadahmad41454
• 0
0
votes
1
reply
119
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 14 days ago by
ATpoint
★ 4.1k • written 14 days ago by
sajadahmad41454
• 0
2
votes
7
replies
442
views
deseq2 results
DESeq2
DifferentialExpression
rnaseqGene
DifferentialRegulation
15 days ago • updated 11 days ago
sajadahmad41454
• 0
1
vote
1
reply
198
views
Handling multiple differential expression comparisons
DESeq2
updated 17 days ago by
Michael Love
42k • written 19 days ago by
Zainab
▴ 10
0
votes
6
replies
312
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 19 days ago by
swbarnes2
★ 1.4k • written 20 days ago by
arfranco
▴ 130
2
votes
3
replies
267
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 18 days ago by
ATpoint
★ 4.1k • written 19 days ago by
Reza
• 0
0
votes
2
replies
180
views
DESeq2 v1.35.0
DESeq2
updated 20 days ago by
Michael Love
42k • written 20 days ago by
Espresso
• 0
0
votes
2
replies
219
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
21 days ago • updated 20 days ago
kcarey
• 0
0
votes
3
replies
235
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 20 days ago by
ATpoint
★ 4.1k • written 21 days ago by
james.zhang20
• 0
1
vote
3
replies
318
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 15 days ago by
ATpoint
★ 4.1k • written 21 days ago by
angelathuynh5
• 0
0
votes
3
replies
309
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 21 days ago by
Michael Love
42k • written 22 days ago by
HAK
• 0
1
vote
1
reply
161
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
updated 22 days ago by
Michael Love
42k • written 22 days ago by
marcusn
• 0
0
votes
2
replies
175
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
23 days ago • updated 20 days ago
Aurora
• 0
0
votes
1
reply
142
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 22 days ago by
Michael Love
42k • written 23 days ago by
Agung
• 0
0
votes
1
reply
142
views
putting my own expression data in deseq2
DESeq2
updated 22 days ago by
ATpoint
★ 4.1k • written 23 days ago by
Dev
• 0
3
votes
3
replies
278
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 24 days ago by
ATpoint
★ 4.1k • written 24 days ago by
bioinf
▴ 10
0
votes
2
replies
231
views
PCA plot suggestions
DESeq2
PCAtools
updated 26 days ago by
Michael Love
42k • written 28 days ago by
Aaliya
• 0
1
vote
3
replies
261
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 26 days ago by
Michael Love
42k • written 27 days ago by
JalapenoCornbread
• 0
2
votes
9
replies
441
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 27 days ago by
James W. MacDonald
66k • written 27 days ago by
Alexandre
• 0
0
votes
1
reply
169
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 28 days ago by
Michael Love
42k • written 28 days ago by
kcarey
• 0
1
vote
3
replies
243
views
Different Shrunk Log2FC according with reference
DESeq2
updated 28 days ago by
Michael Love
42k • written 29 days ago by
andrebolerbarros
▴ 20
1
vote
4
replies
365
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
29 days ago • updated 27 days ago
winwater0928
• 0
3
votes
1
reply
334
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 29 days ago by
Michael Love
42k • written 29 days ago by
i.sudbery
▴ 40
0
votes
8
replies
407
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 28 days ago by
swbarnes2
★ 1.4k • written 29 days ago by
Aaliya
• 0
3
votes
3
replies
260
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 29 days ago by
Michael Love
42k • written 4 weeks ago by
WardDeb
• 0
0
votes
1
reply
160
views
rlog transformation
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
ishij9717
• 0
0
votes
1
reply
133
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 4 weeks ago by
Michael Love
42k • written 5 weeks ago by
09191919
• 0
1
vote
2
replies
764
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
5 weeks ago • updated 26 days ago
Patadù
▴ 10
0
votes
1
reply
151
views
covariate with negative and positive values
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 5 weeks ago by
Brynn
• 0
0
votes
1
reply
171
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
updated 4 weeks ago by
ATpoint
★ 4.1k • written 5 weeks ago by
quanah.hudson
• 0
0
votes
4
replies
273
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
5 weeks ago • updated 4 weeks ago
lonn
• 0
0
votes
3
replies
237
views
Interaction Terms in DESeq2 - Shrinkage
DESeq2
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
andrebolerbarros
▴ 20
1
vote
2
replies
272
views
How to retrieve detailed regression results from deseq2
DESeq2
6 weeks ago • updated 4 weeks ago
Weiqian
▴ 10
0
votes
4
replies
296
views
DEG with low counts
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 5 weeks ago by
Rajat
• 0
0
votes
1
reply
154
views
Deseq2 Rlog transformation
DESeq2
updated 5 weeks ago by
ATpoint
★ 4.1k • written 5 weeks ago by
Sahar
• 0
1
vote
2
replies
217
views
Design Matrix within group comparison more than two groups
DESeq2
5 weeks ago
hemantcnaik
• 0
0
votes
1
reply
155
views
Bioinformatics student
Des
DESeq2
updated 5 weeks ago by
hemantcnaik
• 0 • written 5 weeks ago by
Sahar
• 0
0
votes
1
reply
202
views
DESeq2 gives an error and R crashes
DESeq2
6 weeks ago • updated 5 weeks ago
Shefali
• 0
0
votes
2
replies
250
views
Interaction term vs grouping variable design considerations
DESeq2
DifferentialExpression
updated 6 weeks ago by
Michael Love
42k • written 6 weeks ago by
nhaus
▴ 40
0
votes
1
reply
196
views
IgG control in RIP-seq with Deseq2
DESeq2
RIP-seq
updated 6 weeks ago by
Michael Love
42k • written 6 weeks ago by
Adelaide
• 0
0
votes
2
replies
267
views
Regressing out batch effects
DESeq2
updated 6 weeks ago by
Michael Love
42k • written 6 weeks ago by
kys91240
• 0
50 results • Page
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Answer: Get gene symbols for a set of hg19 coordinates
by
ATpoint
★ 4.1k
Simply download a GTF file (for example from GENCODE) matching the current genome and/or annotation version you are using in your project a…
Comment: Not able to install any Bioconductor 3.18 package [unable to access index]
by
ATpoint
★ 4.1k
Try the mirror suggestion for now: https://support.bioconductor.org/p/9153734/#9153755
Comment: unable to access bioconductor packages - qvalue installation failed...
by
ATpoint
★ 4.1k
Seems to be a general problem at the moment, can reproduce. The the mirror suggestion here: https://support.bioconductor.org/p/9153734/#915…
Comment: Running ENmix with mouse methylation manifest: readidat error - Error in readman
by
Karosham.Reddy
• 0
Thank you for the suggestion the ENmix version is using is ENmix_1.38.01. I tried your suggested code, R identified the idat files but sti…
Comment: pathview error
by
Guido Hooiveld
★ 4.0k
> Will removing the NA values help solve the problem? Did you try? And, FWIW, in the cases I had ensembl-based RNA-seq datasets I removed…
Votes
Comment: recount3 problem downloading data from source sra
Not able to install any Bioconductor 3.18 package [unable to access index]
unable to access bioconductor packages - qvalue installation failed...
Not able to install any Bioconductor 3.18 package [unable to access index]
can't open mghp.osn.xsede.org
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