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Tutorial:
"developing" with HDF5Array
7.9 years ago
Vincent J. Carey, Jr.
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Comment: Mean variance curve troubleshoot filtering
by
gingergeiger22
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0
Gordon, thank for your response. 'filterByExpr()' was marginally better. I can see high variability in samples within the groups from the M…
Comment: What is the importance of `--countReadPairs` in featureCounts on paired end data
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rohitsatyam102
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Since I don't know which organism you are working on, I would recommend using the following:
`-s 0` if library is unstranded
`-C` Discard…
Comment: What is the importance of `--countReadPairs` in featureCounts on paired end data
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rohitsatyam102
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Yes use this option. It is recommended for PE and if you don't use it you will observe nearly twice the number of counts in your results. H…
Comment: Incorporating Salmon offset matrix into Wilcoxon DEG analysis
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Andre
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I edited the previous post. I meant to remove the estimateGLMRobustDisp line in the second snipper :
````
DGE_list_object <- estimateGLMR…
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Sorry, I had forgotten estimateGLMDisp() produces weights as well as NB dispersions. The weights from estimatGLMRobustDisp() were intended …
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