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NanoString
•
reset
0
votes
0
replies
1.2k
views
Trying to use a NanoString expression set with batch effects to do GSVA
GSVA
Normalization
BatchEffect
GSVAdata
NanoString
2.2 years ago
Zhijie
• 0
0
votes
3
replies
2.9k
views
[ExpressionSet] Question: Error in validObject(.Object)
ExpressionSet
Assay
NanoString
Error
Data
updated 2.7 years ago by
James W. MacDonald
68k • written 2.7 years ago by
junli1988
• 0
2
votes
3
replies
2.2k
views
Housekeeping genes vary across contrast groups, using DESeq2 on NanoString data
NanoNormIter
DifferentialExpression
Housekeeping
DESeq2
NanoString
updated 3.2 years ago by
Michael Love
43k • written 3.2 years ago by
argonvibio
• 0
1
vote
5
replies
2.3k
views
how to plot multiple RLEs on same plot?
plotRLE
Nanostring
RUVSeq
DESEQ2
updated 7 months ago by
BioinfGuru
▴ 70 • written 3.8 years ago by
xiaofeiwang18266
▴ 50
0
votes
9
replies
1.9k
views
DESeq2 with nanostring data
deseq2
nanostring
5.3 years ago
acs1990
▴ 10
0
votes
4
replies
1.3k
views
Setting up contrasts with 'limma', patient data, small number of repeats
limma
nanostring
contrasts
updated 5.5 years ago by
Gordon Smyth
52k • written 5.5 years ago by
uridavid.akavia
• 0
0
votes
1
reply
2.0k
views
Application of RUV to a small Nanostring dataset
RUV
Nanostring
RUV-III
updated 4.9 years ago by
hermidalc
▴ 20 • written 5.6 years ago by
raf4
▴ 30
3
votes
6
replies
2.3k
views
Why NanoStringDiff package so slow?
NanoStringDiff
NanoString
nCounter
DE analysis
updated 16 months ago by
georgersmith
• 0 • written 5.6 years ago by
lim6432
▴ 50
16
votes
40
replies
11k
views
Can NanoString data be analyzed using DESeq2?
NanoString
Differential Expressed Genes Analysis
DESeq2
updated 3.2 years ago by
Clara
▴ 10 • written 5.6 years ago by
lim6432
▴ 50
0
votes
5
replies
1.6k
views
NanoStringDiff analysis with confounding factors
differential gene expression
nanostring
confounding factors
updated 6.3 years ago by
James W. MacDonald
68k • written 6.3 years ago by
Guillaume Robert
• 0
1
vote
1
reply
1.8k
views
GSVA with NanoString nCounter data
GSVA
Nanostring
6.7 years ago • updated 6.0 years ago
SB
• 0
9
votes
13
replies
6.6k
views
DESeq2 on NanoString Data
deseq2
nanostring
updated 6.8 years ago by
Michael Love
43k • written 6.8 years ago by
casey.rimland
▴ 170
1
vote
6
replies
2.4k
views
How to access normalized data in the NanoStringDiff package?
nanostringdiff
nanostring
NanoStringDiff
updated 6.8 years ago by
James W. MacDonald
68k • written 6.8 years ago by
casey.rimland
▴ 170
1
vote
1
reply
1.3k
views
DESeq2 confounding cartridge
deseq2
confounders
nanostring
rnaseq
differential gene expression
updated 7.0 years ago by
Michael Love
43k • written 7.0 years ago by
kim.malek88
• 0
0
votes
0
replies
1.6k
views
design matrix and contrast for paired experiment using NanoStringDiff for nCounter data
nanostring
NanoStringDiff
design and contrast matrix
differential gene expression
ncounter
7.7 years ago
c.kohler
• 0
3
votes
8
replies
3.9k
views
Using DESeq2 with Nanostring data (for VST only)
deseq2
variancestabilizingtransformation
nanostring
updated 7.9 years ago by
Michael Love
43k • written 7.9 years ago by
johnmcma
▴ 10
2
votes
0
replies
2.1k
views
NanoString Data Normalization Revisited
nanostring
normalization
9.5 years ago
alakatos
▴ 130
24
votes
12
replies
13k
views
Nanostring analysis with limma
nanostring
limma
updated 9.9 years ago by
ker61
▴ 20 • written 9.9 years ago by
mali salmon
▴ 370
18 results • Page
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Answer: CpGs with genomic positions greater than the chromosome size in EPICv2?
by
James W. MacDonald
68k
All of the annotation packages provided by Bioconductor are simply repackaging of existing data from one or more sources. In this case, the…
Comment: Using TPM from tximport of Salmon output for singscore
by
Kent
• 0
Thank you for the suggestions Michael. Would you say that after filtering out the low count genes, `rlog(counts, blind = T)` is doing the s…
Comment: Using TPM from tximport of Salmon output for singscore
by
Michael Love
43k
For clustering generally we recommend `vst()` see the workflow for example. For working with individual samples, I can see how a scaling/t…
Comment: DESeq2 model - How to test case/control time course model when taking account wi
by
Michael Love
43k
I'd recommend working with a local statistician or someone familiar with linear models in R. I have to reserve my time on the support site …
Comment: deseq2 lfc shrinkage with few DEGs
by
Michael Love
43k
Agree with your edit.
Votes
Comment: Using TPM from tximport of Salmon output for singscore
Answer: Using TPM from tximport of Salmon output for singscore
Comment: deseq2 lfc shrinkage with few DEGs
Answer: DESEQ2 looking at main effects while controlling for a continuous variable
Use of clusterProfiler : Error in testForValidKeytype(x, keytype)
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