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RNASeqData
•
reset
1
vote
3
replies
1.7k
views
Mapping EntrezId to Ensembl IDs returns NA for pseudogenes and snoRNA
RNASeqData
RNASeq
RNASeqR
3.0 years ago
dantasezequiel1
• 0
2
votes
3
replies
1.4k
views
RNA-seq analysis without replicates
DESeq2
RESEQ
RNASeqData
updated 13 months ago by
Cynthia
• 0 • written 13 months ago by
4214811
• 0
0
votes
2
replies
656
views
Is there any reason to think that normalization (e.g. TMM) doesn't work well with samples that that have very different raw counts?
limma
RNASeqData
edgeR
updated 14 months ago by
Hilary
▴ 30 • written 14 months ago by
Lucas
• 0
1
vote
7
replies
844
views
DuplicateCorrelation() block by duplicate sample or by cell line ?
variancePartition
RNASeqData
limma
BulkRNAseq
RNASeq
updated 5 months ago by
Gordon Smyth
50k • written 7 months ago by
Gimena
• 0
1
vote
2
replies
1.1k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 12 months ago by
informatician
• 0
1
vote
2
replies
2.6k
views
Understanding how to contrast two treatments in DESeq2
DESeq2
RNASeqData
updated 20 months ago by
swbarnes2
★ 1.3k • written 20 months ago by
Jennifer M
• 0
3
votes
6
replies
1.3k
views
Different contrast coding strategies in DESeq2 produce slightly different output?
DESeq2
RNASeqData
DifferentialExpression
updated 3.4 years ago by
Michael Love
41k • written 3.4 years ago by
Ben
▴ 50
0
votes
10
replies
2.8k
views
GenomicAlignments - Speeding up readGAlignmentPairs()
Cancer
RNAseqData
GenomicFeatures
Cancer
RNAseqData
GenomicFeatures
updated 9.9 years ago by
Fong Chun Chan
▴ 320 • written 9.9 years ago by
Valerie Obenchain
★ 6.8k
0
votes
1
reply
785
views
Differential Expression Analysis-Input is DESeq Standardized
rnaseq
RNASeqData
limma
limmaGUI
updated 2.1 years ago by
Michael Love
41k • written 2.1 years ago by
sushimoto
• 0
0
votes
6
replies
782
views
Questionable limma results for differential expression on RNA-seq
RNASeqData
DifferentialExpression
limma
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
gld-bioinf
• 0
0
votes
1
reply
1.0k
views
RNASeq analysis - Test for effect of two variables on one (Genotype)
deseq2
multiple factor design
rnaseqdata
updated 8.1 years ago by
Michael Love
41k • written 8.1 years ago by
Dimitris Polychronopoulos
▴ 80
0
votes
5
replies
2.2k
views
Can Differential Isoform expression analysis can be performed using DESeq2 package
RNASeqData
isoform
DESeq2
2.1 years ago • updated 23 months ago
priyanka.m
• 0
6
votes
4
replies
11k
views
How to use combat in order to remove batch effects?
BatchEffect
DESeq2
RNASeqData
updated 2.3 years ago by
BioNovice247
• 0 • written 3.2 years ago by
Emma
▴ 10
3
votes
4
replies
1.4k
views
GEO + RNA-seq. Total Newbie.
RNASeqData
RNASeq
GEO
RNASeqR
HELP
3.1 years ago
hgalmoore
• 0
2
votes
4
replies
2.8k
views
Compare 3 RNASeq groups with no control in DESeq2
anova
RNASeqData
tukeyTest
DESeq2
3.5 years ago • updated 3.4 years ago
ecg1g15
▴ 20
0
votes
1
reply
657
views
Can I transform already normalized rna-seq data with Z-score?
Z-score
RNASeqData
Deseq
DESeq2
updated 7 months ago by
James W. MacDonald
65k • written 7 months ago by
khaserdene99
• 0
0
votes
1
reply
564
views
Controlling for covariates and identifying DEGs using DESeq2
RNASeqData
DESeq2
updated 10 months ago by
Michael Love
41k • written 10 months ago by
Abhishek Singh
▴ 20
1
vote
2
replies
1.6k
views
Binning expression data along with capturing gene identifiers
rnaseqdata
clustering
updated 9.3 years ago by
Johannes Rainer
★ 2.0k • written 9.3 years ago by
Alan Smith
▴ 150
0
votes
2
replies
1.4k
views
How to run vst transformation
RNASeqData
vst
updated 24 months ago by
Michael Love
41k • written 2.0 years ago by
sropri
• 0
0
votes
1
reply
643
views
HSMMSingleCell metadata
ExperimentHubData
ExpressionData
CellDataSet
RNASeqData
2.1 years ago
karl.stamm
▴ 10
2
votes
5
replies
1.4k
views
edgeR Help: Getting no DGEs with an FDR < 0.05
edgeR
RNASeq
RNASeqData
Bioconductor
written 14 months ago by
Rebekah
• 0
1
vote
6
replies
1.4k
views
Contamination in bulkseq samples. Looking at correlated genes?
RNASeqData
DESeq2
rnaseqGene
3.2 years ago
Seaweed
• 0
3
votes
2
replies
931
views
Why some statistically differently expressed genes are not DEGs?
RNASeqData
DEGreport
edgeR
3.2 years ago
Wuschel
▴ 10
0
votes
3
replies
704
views
DESeq: Error in DESeqDataSet(se, design = design, ignoreRank) : some values in assay are negative
RNASeqData
DESeq2
5 months ago
Oenone
• 0
22
votes
15
replies
7.5k
views
biomaRt error unable to fix: need help troubleshooting
biomaRt
RNASeqData
R
updated 5 months ago by
Marie
▴ 10 • written 2.1 years ago by
Dina
▴ 50
1
vote
1
reply
650
views
Strange error while running DESeq() function.
DESeq2
RNASeq
RNASeqData
updated 2.1 years ago by
Michael Love
41k • written 2.1 years ago by
treebig44
▴ 30
4
votes
3
replies
1.2k
views
Replicating iDEP parameters in DESeq2; two conditions and an interaction term.
DESeq2
idep
RNASeqData
updated 2.2 years ago by
swbarnes2
★ 1.3k • written 2.2 years ago by
student2
▴ 10
0
votes
2
replies
347
views
StringDB: Cannot use payload mechanism to colour nodes
RNASeqData
STRINGdb
DifferentialExpression
GraphAndNetwork
Network
4 months ago
Luca
• 0
0
votes
1
reply
559
views
How to find degraded genes but unaffected by condition in a time course RNAseq experiment ?
DESeq2
RNASeqData
DifferentialExpression
updated 15 months ago by
ATpoint
★ 4.1k • written 15 months ago by
Julie
• 0
1
vote
1
reply
598
views
DESEQ2 differential expression for subsets of data?
RNASeqData
DESeq2
updated 10 months ago by
ATpoint
★ 4.1k • written 10 months ago by
amnahsiddiqa
▴ 10
0
votes
2
replies
1.2k
views
lncRNA Genes in a Dataset
ncRNAtools
RNASeqData
2.1 years ago
sushimoto
• 0
2
votes
8
replies
2.3k
views
While trying to remove non differentially expressed genes through limma, I am getting a warning
RNASeqData
updated 3.1 years ago by
Gordon Smyth
50k • written 3.1 years ago by
Rishav
▴ 20
0
votes
6
replies
1.2k
views
while doing co-expression network analysis, I am getting an error
RNASeqData
3.1 years ago
Rishav
▴ 20
2
votes
4
replies
1.2k
views
DESeq2: how to integrate a manually edited matrix into full argument of DESeq
RNASeqData
DESeq2
2.7 years ago
khaveh
▴ 10
0
votes
3
replies
753
views
Understanding edgeR differential expression results
Transcriptomics
edgeR
DifferentialExpression
RNASeqData
RNASeq
updated 14 months ago by
Gordon Smyth
50k • written 14 months ago by
Teresa
• 0
1
vote
2
replies
523
views
I changed the reference in DGE contrasts by DESeq2, but the results changed just a bit and not in the sign of Log2FC (positive to negative and invers…
RNASeqData
DESeq2
updated 9 months ago by
Michael Love
41k • written 9 months ago by
mohammad.hossein.banabazi
• 0
1
vote
4
replies
1.0k
views
lncRNA Differential Expression Analysis
DESeq2
limma
RNASeqData
2.1 years ago
sushimoto
• 0
0
votes
4
replies
665
views
filtering out lowly expressed genes by groups
RNASeqData
DESeq2
updated 7 months ago by
James W. MacDonald
65k • written 7 months ago by
sohail.mbio
▴ 10
7
votes
5
replies
3.4k
views
Normalise to a housekeeping gene in DESEq2
DESeq2
housekeeping
R
RNASeqData
3.4 years ago
ecg1g15
▴ 20
2
votes
9
replies
2.3k
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA
r
RNASeqData
voom
limma
22 months ago
Peipei
• 0
1
vote
4
replies
1.3k
views
Inverted Log2FC values after DEseq2 analysis
DESeq2
Log2FC
RNASeqData
16 months ago
Iwan
• 0
3
votes
3
replies
1.4k
views
How to formulate 2 factor nested design in DESeq2
nested_design
RNASeqData
DESeq2
23 months ago
JK Kim
▴ 20
0
votes
1
reply
108
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 4 days ago by
Gordon Smyth
50k • written 4 days ago by
z_bahari
• 0
5
votes
6
replies
557
views
Monotonic genes in results of differential gene expression analysis
DifferentialExpression
rnaseqGene
RNASeqData
3 months ago
Shaimaa Gamal
• 0
1
vote
1
reply
1.1k
views
Help understanding edgeR code for doing exactTest
exacttest
RNASeqData
edgeR
diffGeneAnalysis
updated 2.5 years ago by
Gordon Smyth
50k • written 2.5 years ago by
Julia
• 0
1
vote
1
reply
335
views
Differential gene expression analysis
RNASeqData
RNAseq123
updated 5 months ago by
ATpoint
★ 4.1k • written 5 months ago by
kaurprab
• 0
1
vote
2
replies
672
views
Installation of R-packages for RNA-seq analysis
RNASeqData
14 months ago
Begin-R
• 0
0
votes
2
replies
631
views
RNA-Seq and disease outcome analysis
rnaseqAnalysis
RNASeqData
14 months ago
JonasEnder
• 0
8
votes
2
replies
6.9k
views
handling outliers with DESeq2
RNASeqData
DESeq2
Outliers
3.0 years ago
paty
▴ 60
1
vote
1
reply
398
views
Deseq contrast question
RNASeqData
DESeq2
updated 10 months ago by
Michael Love
41k • written 11 months ago by
Hannah
▴ 10
50 results • Page
1 of 1
Recent ...
Replies
Comment: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
by
Hervé Pagès
16k
Indeed. Calculations involving floating point arithmetic are architecture-dependent, and testing the results should be done with `all.equal…
Comment: How to use bootRanges to bootstrap small RNA loci (nullranges package)
by
Poonam
• 0
I will follow what you suggested. I didn't use max gap anywhere. I was following statistic I and regarding the size of features, my small…
Comment: How to use bootRanges to bootstrap small RNA loci (nullranges package)
by
Michael Love
41k
Oh I see, for the second question, I don't have a great answer. I typically think of one set as the anchor, whose ascertainment is driving …
Comment: Help with running egsea()
by
James W. MacDonald
65k
Oh, right. Ideally you would use NCBI (aka Entrez gene) IDs because they are way more likely to be unique. Gene symbols are broken down int…
Comment: Help with running egsea()
by
Chris
• 0
Thanks James! I update the question. The last question mean when we use `buildIdx()`, could we use gene symbol instead of entrezID. However…
Votes
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Comment: Help with running egsea()
Answer: Trim/Filter out-of-bounds GRanges
Comment: Help with running egsea()
Answer: How to save the DEXSeq results
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