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DeSeq2
•
reset
1
vote
1
reply
152
views
Subsetting data verses contrasts [DESeq2]?
DESeq2
updated 12 days ago by
ATpoint
★ 5.0k • written 12 days ago by
js101
• 0
0
votes
0
replies
88
views
How to start learning RNA sequence analysis with DESeq2
DESeq2
13 days ago
Syed
• 0
1
vote
4
replies
331
views
Bulk RNAseq analysis for
DESeq2
RNASeq
edgeR
limma
14 days ago • updated 13 days ago
Mariami
• 0
0
votes
5
replies
2.3k
views
DESeq2 contrast cleaning problem
software error
bugs
deseq2
written 10.1 years ago by
john.hu
• 0
0
votes
1
reply
188
views
DESeq2 for 16S rRNA Analysis
DESeq2
updated 24 days ago by
James W. MacDonald
68k • written 24 days ago by
lizflor
• 0
0
votes
5
replies
298
views
Lowly expressed genes reported as significant when using lfcThreshold in DESeq
RNASeq
DESeq2
DifferentialExpression
updated 27 days ago by
Michael Love
43k • written 27 days ago by
Ahmed Salah
• 0
0
votes
2
replies
242
views
lfc shrinkage estimator in DESeq2
DESeq2
written 28 days ago by
julia_mcdonough
• 0
0
votes
13
replies
909
views
Making a Full Rank Model for Allele-Specific Expression with DESeq2
DESeq2
updated 27 days ago by
Gordon Smyth
53k • written 4 weeks ago by
chp265
• 0
0
votes
6
replies
718
views
BiocParallel (and DESeq2) - wrong args for environment subassignment
BiocParallel
DESeq2
updated 4 weeks ago by
Martin Morgan
25k • written 11 weeks ago by
Benjamin
• 0
0
votes
1
reply
330
views
DESeq2 - how to shrink LFC for pairwise comparisons having multiple groups
shrinkage
DESeq2
contrast
updated 4 weeks ago by
ATpoint
★ 5.0k • written 5 weeks ago by
ja569116
• 0
0
votes
1
reply
175
views
News:
Online RNA-seq analysis course 26-29th Janury
EnhancedVolcano
GSEABase
pheatmap
Workshop
DESeq2
written 6 weeks ago by
Frances Turner
▴ 20
0
votes
3
replies
461
views
DESeq2 - how many surrogate variables from svaseq, use of lfcshrink
sva
lfcshrink
DESeq2
9 weeks ago • updated 5 weeks ago
ja569116
• 0
3
votes
6
replies
1.1k
views
Highly similar RNA-seq samples in PCA - pooling or technical duplication?
RNASeq
DESeq2
DifferentialExpression
RNASeqData
written 3 months ago by
Ahmed Salah
• 0
0
votes
1
reply
265
views
Question about DESeq2 Design for Paired Treatment Study
DESeq2
updated 12 weeks ago by
ATpoint
★ 5.0k • written 12 weeks ago by
Junmo
• 0
0
votes
2
replies
1.3k
views
Install old version of DESeq2
apeglm
Install
DESeq2
version
updated 3 months ago by
Kevin Blighe
★ 4.0k • written 7 months ago by
Asma
• 0
1
vote
11
replies
2.4k
views
Using DESeq2 on single cell RNA Seq data of same cell type but different conditions that were pseudo bulked
DESeq2
scRNAseq
updated 3 months ago by
Kevin Blighe
★ 4.0k • written 6 months ago by
Raya
• 0
0
votes
2
replies
439
views
DESeq2 on non-RNASeq counts data
DESeq2
written 6 months ago by
vs
• 0
0
votes
2
replies
407
views
3-factor DESeq2 trime-series analysis
time-series
DESeq2
updated 3 months ago by
Kevin Blighe
★ 4.0k • written 5 months ago by
gogeni5529
• 0
0
votes
1
reply
266
views
[DESeq2] Changing dispersion estimates between runs of DESeq2
DESeq2
3 months ago • updated 11 days ago
colinmccornack
• 0
0
votes
4
replies
365
views
Getting a large intercept with DESeq2
DESeq2
DifferentialExpression
TimeCourse
3 months ago
Denise
• 0
0
votes
2
replies
428
views
Setting up a DESeq2 Design with Paired Data
DESeq2
3 months ago
Alexander
• 0
2
votes
4
replies
618
views
DESeq2 interactions
DESeq2
updated 3 months ago by
Michael Love
43k • written 5 months ago by
Michael
• 0
3
votes
14
replies
1.5k
views
DESeq2 used with zinbwave weights - Differences on contrasts calculations
zinbwave
DESeq2
written 6 months ago by
Aurélie
• 0
0
votes
3
replies
462
views
Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
RUVSeq
DESeq2
updated 5 months ago by
Michael Love
43k • written 5 months ago by
yongjie.wang
• 0
0
votes
1
reply
983
views
Which of apeglm and ashr may be more appropriate for pseudobulked DESeq2 analysis of single-cell RNA-seq data?
apeglm
DESeq2
updated 5 months ago by
Michael Love
43k • written 6 months ago by
KS
• 0
3
votes
5
replies
1.3k
views
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
DESeq2
updated 5 months ago by
James W. MacDonald
68k • written 6 months ago by
snijesh
▴ 200
17
votes
45
replies
14k
views
Can NanoString data be analyzed using DESeq2?
NanoString
Differential Expressed Genes Analysis
DESeq2
updated 6 months ago by
James W. MacDonald
68k • written 6.6 years ago by
lim6432
▴ 60
0
votes
11
replies
850
views
Getting way too many DEGs with LRT for time-series RNA-seq!
TimeCourse
DESeq2
6 months ago
Denise
• 0
2
votes
6
replies
708
views
Very different log2 Fold changes with DESeq2 and explict calculation
DESeq2
6 months ago
richardallenfriedmanbrooklyn
▴ 30
0
votes
3
replies
384
views
DESeq2 inconsistent fits depending on model design with interacting factors
des
DESeq2
written 6 months ago by
fedor.bezrukov
▴ 10
0
votes
4
replies
1.2k
views
Using shrunken LFC and s-value from DESeq2 as DGE results
apeglm
s-value
DESeq2
updated 6 months ago by
Michael Love
43k • written 7 months ago by
Erika
• 0
1
vote
2
replies
585
views
Differential expression on the transcript-level
DESeq2
updated 7 months ago by
swbarnes2
★ 1.4k • written 7 months ago by
泓朴
• 0
3
votes
13
replies
2.4k
views
Highly DE genes but small count difference within conditions
RNASeq
DESeq2
RNASeqData
written 16 months ago by
bioshot.com
• 0
4
votes
8
replies
2.2k
views
Should Xenium pseudobulk experiments be analysed like microarrays (limma) or like RNASeq (edgeR,Deseq2, or limma-voom)
Deseq2
edgeR
limma
xenium
Voom
7 months ago
richardallenfriedmanbrooklyn
▴ 30
7
votes
9
replies
5.9k
views
competitive enrichment test yields too few significant results, self-contained too many
limma
DESeq2
fry
edgeR
7 months ago
bbao
▴ 10
0
votes
1
reply
635
views
discrepancy in IDs from file and tx2gene (IDs don't match)
tximport
DESeq2
updated 7 months ago by
James W. MacDonald
68k • written 7 months ago by
IUH
• 0
0
votes
2
replies
1.1k
views
gene detection across experiments
DESeq2
Normalization
7 months ago
Mara
• 0
5
votes
11
replies
4.2k
views
parameters of filterByExpr
edgeR
DESeq2
filterByExpr
written 9 months ago by
caroline.zanchi
▴ 10
1
vote
5
replies
5.6k
views
Pre-filtering, adjusting independent filtering, or lfcthreshold() on DESeq2? Low counts and dropouts produce interesting volcano plots.
DESeq2
updated 7 months ago by
Michael Love
43k • written 8 months ago by
Alexander
• 0
1
vote
1
reply
2.3k
views
DESeq2 LRT: Testing Effects of Treatment1, Treatment2, and Their Interaction in a Four-Factor Design
DESeq2
multifactorialdesign
interaction
InteractionSet
updated 8 months ago by
Michael Love
43k • written 8 months ago by
Integrative
• 0
0
votes
2
replies
1.3k
views
6 groups to compare DESeq2 (Wald vs LRT)
DESeq2
updated 8 months ago by
Michael Love
43k • written 8 months ago by
Rocio
• 0
3
votes
2
replies
1.6k
views
Biological replicates consideration in DESeq2 design formula.
DESeq2
RNASeqData
RNASeq
written 8 months ago by
Christian
▴ 20
1
vote
2
replies
2.8k
views
Deseq2 paired sample in the design is considered as "batch"?
DESeq2
written 8 months ago by
corranna98
• 0
3
votes
1
reply
3.9k
views
DESeq2 empirical bayes: variance of the prior for log fold changes (LFC)
DESeq2
updated 8 months ago by
Michael Love
43k • written 8 months ago by
Chenghao
▴ 10
0
votes
1
reply
686
views
DESeq2 results
result
DESeq2
subsetting
updated 8 months ago by
ATpoint
★ 5.0k • written 8 months ago by
rajdeepboral00
• 0
0
votes
1
reply
509
views
Unclear DEseq2 replaceOutliers error message
DESeq2
updated 8 months ago by
Michael Love
43k • written 8 months ago by
Pak
• 0
0
votes
1
reply
686
views
Difference in DESeq2 output and tidybulk DESeq2 method
DifferentialExpression
DESeq2
tidybulk
RNASeq
updated 9 months ago by
Michael Love
43k • written 9 months ago by
s.malik
• 0
3
votes
3
replies
2.2k
views
Deseq2 DE analysis of host-pathogen samples (model separately or jointly?)
DESeq2
written 9 months ago by
dudutchy
▴ 10
2
votes
3
replies
1.7k
views
Differential gene expression analysis on haplotype-resolved diploid assembly
edgeR
DESeq2
haplotype
limma
9 months ago
Paulito
• 0
4
votes
4
replies
2.3k
views
Adding gene names as row annotations to an RNAseq heatmap made with ComplexHeatmap
DESeq2
ComplexHeatmap
9 months ago
caroline.zanchi
▴ 10
4,851 results • Page
1 of 98
Recent ...
Replies
Comment: Question on processing compound protein groups in limpa
by
JKim
• 0
Many thanks. I appreciate your advice.
Answer: Question on processing compound protein groups in limpa
by
Gordon Smyth
53k
Different people have different ways of dealing with compound protein groups. You could just leave them in the analysis, in which case limp…
Answer: Correct usage of limpa for protein input
by
Gordon Smyth
53k
limpa can work on protein-level quantifications, but they must be intensity-based and must be on the log scale. From what I read (<https://…
Comment: A problem occurs when running the monocle2
by
a2a2hset32aa
• 0
Troubleshooting monocle2 errors in R 4.5.1 often points to package incompatibilities, particularly with newer versions of igraph. For resea…
Comment: Deadline Extended: EuroBioC2026 Call for Abstracts
by
a2a2hset32aa
• 0
Attending EuroBioC2026 is an excellent way for researchers to explore the intersection of technology and biology. For those seeking high qu…
Votes
Answer: Question on processing compound protein groups in limpa
Answer: Correct usage of limpa for protein input
Answer: Correct usage of limpa for protein input
Answer: Correct usage of limpa for protein input
A: Interpretaion of dendrogram height in WGCNA?
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