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DESeq2
•
reset
0
votes
0
replies
13
views
Deseq2 DE analysis of host-pathogen samples (model separately or jointly?)
DESeq2
3 hours ago
dudutchy
• 0
0
votes
1
reply
163
views
DESeq2 Apeglm Coefficient Reordering / Log2FoldChange Comparison Change
DESeq2
apeglm
updated 13 hours ago by
James W. MacDonald
68k • written 1 day ago by
sk
• 0
1
vote
5
replies
800
views
Heatmap of a transformed deseq object with pheatmap
DESeq2
heatmaps
updated 13 hours ago by
James W. MacDonald
68k • written 1 day ago by
caroline.zanchi
• 0
0
votes
0
replies
40
views
Difference in DESeq2 output and tidybulk DESeq2 method
DifferentialExpression
DESeq2
tidybulk
RNASeq
1 day ago
s.malik
• 0
0
votes
4
replies
926
views
DESEQ2 - Correctly altering log2foldchange when switching comparison direction (HELP GREATLY APPRECIATED)
DESeq2
9 days ago • updated 3 days ago
sk
• 0
8
votes
3
replies
2.3k
views
DESeq2 class error: superclass "ExpData" not defined in the environment of the object's class
DESeq2
updated 5 days ago by
Michael Love
43k • written 8 days ago by
Cassia
▴ 40
1
vote
5
replies
826
views
parameters of filterByExpr
edgeR
DESeq2
filterByExpr
updated 8 days ago by
Gordon Smyth
52k • written 11 days ago by
caroline.zanchi
• 0
0
votes
3
replies
606
views
Discrepancies in normalised count data vs unnormalised
DESeq2
updated 8 days ago by
swbarnes2
★ 1.4k • written 13 days ago by
AMgroup
• 0
2
votes
3
replies
1.3k
views
Filtering after DESeq
DESeq2
IHW
updated 8 days ago by
ChaseWendrix
• 0 • written 18 days ago by
caroline.zanchi
• 0
6
votes
4
replies
7.5k
views
DESeq2 with proteomics data
deseq2
updated 11 days ago by
wook2014
• 0 • written 6.8 years ago by
shyu
▴ 10
4
votes
4
replies
2.7k
views
Cannot extract size factor from DESeq2 analysis
deseq2
tximport
updated 13 days ago by
s.malik
• 0 • written 5.0 years ago by
zroger499
• 0
0
votes
2
replies
470
views
Design formula for circadian time-course data for vst() function
DESeq2
14 days ago
Vishnu
• 0
0
votes
2
replies
591
views
Analyzing TnSeq data with DESeq2
DESeq2
written 20 days ago by
newfunland
• 0
8
votes
10
replies
16k
views
GSEA preranked analysis downstream of DEseq2
deseq2
gsea
rnaseq
updated 15 days ago by
ATpoint
★ 4.8k • written 8.8 years ago by
EJ
▴ 20
0
votes
3
replies
2.0k
views
Controlling for batch and estrus cycle using DESeq2 in RNA-seq analysis
deseq2
random effects
batch effects
RNA-seq
rna-seq
updated 18 days ago by
donnycrimson
• 0 • written 7.8 years ago by
Dipro
• 0
0
votes
2
replies
2.8k
views
DESeq2 Design controlling for gender
deseq2
multiple factor design
updated 18 days ago by
donnycrimson
• 0 • written 7.7 years ago by
rejane.troudet
• 0
0
votes
2
replies
499
views
Differing results with DESeq2
DESeq2
updated 19 days ago by
JKim
• 0 • written 5 weeks ago by
hampsdiv
• 0
0
votes
2
replies
366
views
RNA Seq using Lrt
DESeq2
21 days ago • updated 20 days ago
Rob
• 0
2
votes
3
replies
2.3k
views
How to convert transcript counts to gene counts in R?
DESeq2
tximport
updated 20 days ago by
ben
• 0 • written 2.6 years ago by
L
▴ 50
0
votes
1
reply
899
views
How do I set up my DESeq analysis when I know a few vehicle control samples provide background effects we don't want to see in any of the subsequent …
DESeq2
RUVSeq
updated 21 days ago by
Michael Love
43k • written 7 weeks ago by
Simran
• 0
0
votes
3
replies
1.7k
views
DeSeq Strategies for run to run Replication
DESeq2
Normalization
Clustering
replicate
updated 24 days ago by
Michael Love
43k • written 2.7 years ago by
Giorgio
• 0
0
votes
3
replies
758
views
Log2foldchange strange behavior
DESeq2
updated 29 days ago by
ATpoint
★ 4.8k • written 29 days ago by
RambakKulit
• 0
0
votes
1
reply
605
views
WGCNA
WGCNA
DESeq2
updated 4 weeks ago by
ATpoint
★ 4.8k • written 5 weeks ago by
bioinformaticssrm2011
• 0
0
votes
1
reply
874
views
Comparison of results obtained from a subset of the data versus the complete dataset for a few selected genes.
DESeq2
updated 4 weeks ago by
swbarnes2
★ 1.4k • written 4 weeks ago by
Bioinformatician_R
▴ 20
1
vote
1
reply
1.2k
views
PhD scholar
RNASeqAnalysis
DESeq2
updated 4 weeks ago by
shepherl
4.1k • written 4 weeks ago by
Richa
• 0
0
votes
2
replies
729
views
DESeq2 lfcShrink drastic shrinkage
DESeq2
updated 4 weeks ago by
Nick
• 0 • written 15 months ago by
theophile
• 0
1
vote
8
replies
2.3k
views
[ChIP-seq] DESeq2 on counts matrix from union set of called peaks
csaw
ChIPseq
DESeq2
DiffBind
9 weeks ago • updated 4 weeks ago
Luca
• 0
0
votes
1
reply
330
views
DESeq2 - am I extracting results incorrectly?
DESeq2
updated 5 weeks ago by
James W. MacDonald
68k • written 5 weeks ago by
bd2000
▴ 30
1
vote
3
replies
813
views
DESeq - help with pair wise comparisons
DESeq2
5 weeks ago
bd2000
▴ 30
1
vote
8
replies
2.6k
views
DESeq: Gene is not flagged as differentially expressed despite count data showing differences
DifferentialExpression
DESeq2
5 weeks ago
ktz
• 0
0
votes
11
replies
2.0k
views
Question about using LRT test for time course experiments with DESeq2
DESeq2
updated 5 weeks ago by
Rob
• 0 • written 6 months ago by
Denise
• 0
5
votes
30
replies
6.0k
views
DESeq2 model - How to test case/control time course model when taking account within sample variability?
deseq2
multiple time points
time course
casecontrol
model design
updated 6 weeks ago by
wuwenhui2
• 0 • written 7.9 years ago by
heikki.sarin
▴ 10
0
votes
1
reply
579
views
DESeq2
org.Mmu.eg.db
DESeq2
TimeCourse
DMSOvsdrug
RNASeqData
updated 6 weeks ago by
ATpoint
★ 4.8k • written 6 weeks ago by
Cosmic
• 0
4
votes
6
replies
1.3k
views
Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
updated 6 weeks ago by
ATpoint
★ 4.8k • written 9 weeks ago by
Elijah
• 0
1
vote
4
replies
498
views
DESEQ2 looking at main effects while controlling for a continuous variable
DESeq2
7 weeks ago
Lennie
• 0
1
vote
3
replies
5.7k
views
DESeq2 estimateSizeFactors with control genes
deseq2
differential gene expression
controls
references
updated 7 weeks ago by
sofia.rigou
• 0 • written 6.5 years ago by
cris.kgs
• 0
2
votes
4
replies
1.7k
views
deseq2 lfc shrinkage with few DEGs
RNASeq
DESeq2
updated 7 weeks ago by
Michael Love
43k • written 2.9 years ago by
Victor
▴ 10
38
votes
23
replies
17k
views
DESeq2 testing ratio of ratios (RIP-Seq, CLIP-Seq, ribosomal profiling)
deseq2
10.7 years ago • updated 7 weeks ago
Michael Love
43k
0
votes
4
replies
720
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2
TechnicalReplicates
RNASeq
written 8 weeks ago by
questionanswer
• 0
0
votes
2
replies
494
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 8 weeks ago by
Michael Love
43k • written 9 weeks ago by
rohitsatyam102
▴ 20
1
vote
1
reply
825
views
Use of org.Rn.eg.db with GRCr8 reference genome
DESeq2
org.Rn.eg.db
updated 8 weeks ago by
James W. MacDonald
68k • written 8 weeks ago by
Erika
• 0
1
vote
5
replies
4.1k
views
DESeq2 swap ensembl gene id rownames with gene symbol column
deseq2
dataframe
ensembl gene id
gene symbol
entrezid
updated 8 weeks ago by
Erika
• 0 • written 5.4 years ago by
jason.williams
▴ 10
1
vote
6
replies
1.9k
views
how to analysis htseq-count
deseq2
written 7.3 years ago by
Bioinformatics
▴ 30
1
vote
3
replies
5.1k
views
Log2 fold change value in DESeq2
deseq2
rnaseq
written 7.6 years ago by
AP
• 0
5
votes
3
replies
28k
views
DESeq2: thousands of NA for pvalues?
deseq2
rna-seq
DEGs
cooks cutoff
written 6.3 years ago by
aishu.jp
▴ 30
0
votes
1
reply
242
views
DESeq use in lipidomic analysis
DESeq2
package
Lipidomics
updated 9 weeks ago by
Michael Love
43k • written 9 weeks ago by
泓宇
• 0
0
votes
3
replies
693
views
how many significant figures are there in DESeq2 output
DESeq2
updated 10 weeks ago by
ATpoint
★ 4.8k • written 10 weeks ago by
Matthew McCormack
▴ 180
2
votes
6
replies
1.8k
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 10 weeks ago by
Jane
• 0 • written 12 months ago by
Reza
• 0
2
votes
5
replies
1.1k
views
Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
10 weeks ago
picasa1983
• 0
3
votes
7
replies
2.1k
views
Vertical line at A=0, nothing else, on MA plot
deseq2
updated 10 weeks ago by
melissa
▴ 10 • written 6.2 years ago by
david.wheeler7
▴ 20
50 results • Page
1 of 1
Recent ...
Replies
Comment: How to extract coordinates of mismatches from a BAM file lodaded in as a GAlignm
by
Charles
• 0
I am trying this but it is slow. ``` aln2mismatches <- function (gal) { gr <- GRanges(gal) irl <- cigarRangesAlongReferenceSpace(cigar…
Answer: DESeq2 Apeglm Coefficient Reordering / Log2FoldChange Comparison Change
by
James W. MacDonald
68k
Yes, you can simply flip the sign of the logFC to reverse the direction of the comparison.
Comment: Heatmap of a transformed deseq object with pheatmap
by
James W. MacDonald
68k
The argument for annotation_col is meant to be a `data.frame`, not a vector. The hint in the error is that it has the wrong number of dimen…
Comment: Heatmap of a transformed deseq object with pheatmap
by
caroline.zanchi
• 0
I used this argument in the second paragraph of code forthe heatmap, this is wehre I got the message Error in `[.default`(annotation_col, c…
Answer: Heatmap of a transformed deseq object with pheatmap
by
James W. MacDonald
68k
Ah, yes. The non-ASCII character error. My favorite! Please note that `pheatmap` is not a Bioconductor package, so you should be asking …
Votes
Comment: Heatmap of a transformed deseq object with pheatmap
DESeq2: Question about the alpha in summary() and results()
Comment: How to load scRNA-seq data from GEO
limma correction for tumor purity
limma correction for tumor purity
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