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Recent ...
Replies
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
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I moved your post from an "answer" to a "comment".
I don't know about `offst()` function, what is the scale or direction, or whether you a…
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@atpoint Should I first standardise data and then average replicates?
Comment: 3-factor DESeq2 trime-series analysis
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Thans you @swbarnes2 for the answer.
I can see why trying to include all three parameters might be difficult to interpret. But we're inte…
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swbarnes2
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What does your PCA look like? A lot of times, I find that different cell lines are so different from each other, that they should not be d…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
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Thanks for your reply.
" normFactors <- exp(-1 * offst(set2))
normFactors <- normFactors / exp(rowMeans(log(normFactors)))
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