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design matrix
•
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18
votes
37
replies
17k
views
Correct construction of design matrix in limma for multiple contrasts for gene expression profiling
limma
illumina
differential gene expression
multiple comparisons
design matrix
8.9 years ago • updated 8.8 years ago
svlachavas
▴ 830
1
vote
18
replies
2.0k
views
Microarray expression analysis for on factor taking account confounders (covariables)
limma
design matrix
matrix with covariables
updated 6.2 years ago by
Aaron Lun
★ 28k • written 6.2 years ago by
michel655
• 0
1
vote
17
replies
1.8k
views
design for edgeR
edger
design matrix
glmlrt
updated 7.4 years ago by
Aaron Lun
★ 28k • written 7.4 years ago by
simarsidhu25
• 0
11
votes
14
replies
4.3k
views
Setting up the Design Matrix
rnaseq
edgeR
multiple factor design
design matrix
8.3 years ago
Yogender Gowtham
▴ 20
2
votes
13
replies
1.9k
views
problem about (design matrix)
limma
limma design matrix
design matrix
updated 8.2 years ago by
Aaron Lun
★ 28k • written 8.2 years ago by
libya.tahani
• 0
2
votes
13
replies
2.1k
views
LIMMA : group specific effect of a treatment while controlling for individual effect
limma-voom
design matrix
limma time-course
limma design matrix
limma
6.2 years ago
SPbeginner
• 0
5
votes
12
replies
2.0k
views
how to use edgeR analysis RNA-seq data which has four factors
edger
design matrix
comparing more than two groups
multiple factor design
8.3 years ago • updated 8.1 years ago
xiaoaozqd
• 0
3
votes
12
replies
2.8k
views
Questions about complex design in limma regarding an agilent microarray dataset with multiple time points and different biological replicates
limma
design matrix
multiple time points
agilent microarrays
updated 8.2 years ago by
Aaron Lun
★ 28k • written 8.2 years ago by
Konstantinos Yeles
▴ 80
7
votes
11
replies
3.3k
views
Continuous variable interaction with Groups in design matrix
edger
design matrix
updated 8.3 years ago by
Aaron Lun
★ 28k • written 8.3 years ago by
jhj89
▴ 10
0
votes
11
replies
3.2k
views
Limma Design Matrix for Paired Samples with Confouding Factors
limma
design matrix
paired samples
confounding factors
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
yuabrahamliu
• 0
2
votes
10
replies
2.1k
views
Design matrix for unbalanced data
edgeR
RNA-Seq
design matrix
multifactorial design
8.2 years ago
Yogender Gowtham
▴ 20
2
votes
10
replies
2.8k
views
Time-series analysis with DESeq2
deseq2
time-series
design matrix
edger
updated 5.2 years ago by
Michael Love
42k • written 5.2 years ago by
Assa Yeroslaviz
★ 1.5k
2
votes
10
replies
1.8k
views
Am I on the right tracks with a 3 factor design in Limma?
r
limma
design matrix
biobase
updated 5.8 years ago by
Gordon Smyth
50k • written 5.8 years ago by
reubenmcgregor88
• 0
4
votes
9
replies
1.2k
views
Statistical background for computing intial estimates for the means of negative binomially distributed gene counts in DESeq2
deseq2
design matrix
5.3 years ago
nikosbosse
• 0
0
votes
8
replies
3.9k
views
Design question limma multi variables
limma
design matrix
updated 7.3 years ago by
Aaron Lun
★ 28k • written 7.3 years ago by
Stane
▴ 40
4
votes
8
replies
2.5k
views
Can't find any differentially expressed genes
rnaseq
differential gene expression
design matrix
edgeR
updated 9.1 years ago by
Gordon Smyth
50k • written 9.1 years ago by
s3462450
▴ 10
3
votes
8
replies
1.3k
views
Design matrix for a two-color experimental design testing for time effect on 3 treated cell-lines, each with its own time=0 control
limma
microarray
design matrix
twochannel
updated 5.8 years ago by
Gordon Smyth
50k • written 5.8 years ago by
rubi
▴ 110
1
vote
8
replies
1.6k
views
Design matrix for regressing gene expression on a continuous variable - age
limma
lmfit
design matrix
r
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
anupriya.dalmia
• 0
2
votes
8
replies
3.7k
views
EdgeR. Analysis with pairing across condition and treatment. Design Matrix
edger
ANOVA
Design matrix
updated 9.3 years ago by
Gordon Smyth
50k • written 9.3 years ago by
chwhite
• 0
3
votes
7
replies
1.6k
views
single continuous factor
edger
design matrix
continuous
updated 2.2 years ago by
Tongjun
• 0 • written 6.0 years ago by
ashley.lu
• 0
5
votes
7
replies
3.6k
views
Deseq2 design for one condition over 8 different time point
deseq2
rna-seq
design matrix
deseq
updated 5.6 years ago by
Michael Love
42k • written 5.6 years ago by
thindmarsmission
▴ 10
4
votes
7
replies
1.1k
views
Design matrix and contrast for RNA knockdown experiment
design matrix
limma
contrast matrix
updated 7.6 years ago by
Aaron Lun
★ 28k • written 7.6 years ago by
maltethodberg
▴ 180
3
votes
7
replies
2.0k
views
complex design for chip-seq analysis
chipseq
csaw
limma
design matrix
multiple factor design
updated 6.5 years ago by
Aaron Lun
★ 28k • written 6.5 years ago by
Assa Yeroslaviz
★ 1.5k
0
votes
7
replies
2.4k
views
DESeq2: how to identify confounding variable in the design matrix
deseq2
confounders
design matrix
rnaseq
7.0 years ago
lina.faller
▴ 10
3
votes
6
replies
1.7k
views
Design that evaluates response to continuous and categorical variables
limma
design and contrast matrix
design matrix
limma design matrix
updated 8.9 years ago by
James W. MacDonald
65k • written 8.9 years ago by
Moritz E. Beber
▴ 10
3
votes
6
replies
1.3k
views
EdgeR model formula design matrix
edger
design matrix
paired samples
updated 6.1 years ago by
Aaron Lun
★ 28k • written 6.1 years ago by
aiko
• 0
2
votes
6
replies
3.3k
views
RNA-seq paired analysis design
paired samples
design matrix
limma
deseq2
edger
updated 7.3 years ago by
Gordon Smyth
50k • written 7.3 years ago by
cjmb3
• 0
3
votes
6
replies
1.0k
views
Overlapping treatments in the design matrix
edger
design matrix
updated 8.3 years ago by
Aaron Lun
★ 28k • written 8.3 years ago by
jhj89
▴ 10
5
votes
5
replies
1.4k
views
Design and Contrast Matrix for Linear Model. To Combine or not to Combine?
limma
design and contrast matrix
design matrix
contrast matrix
updated 5.6 years ago by
James W. MacDonald
65k • written 5.6 years ago by
mikhael.manurung
▴ 270
0
votes
5
replies
1.1k
views
differential expression analysis of cell subtypes mixture
deseq2
edgeR
design matrix
multifactorial design
updated 6.1 years ago by
Aaron Lun
★ 28k • written 6.1 years ago by
Assa Yeroslaviz
★ 1.5k
2
votes
5
replies
3.8k
views
design model matrix for nested factors (edgeR)
edger
design matrix
model matrix
updated 9.1 years ago by
Aaron Lun
★ 28k • written 9.1 years ago by
silvia.paolucci81
• 0
0
votes
5
replies
1.7k
views
DESeq2 package : Error in checkFullRank(modelMatrix) while designing multi factor
DESeq2
multiple factor design
design model
design matrix
rna-seq
updated 5.4 years ago by
Michael Love
42k • written 5.4 years ago by
iammiso
• 0
2
votes
5
replies
847
views
a complicate scenario for Design Matrix
edgeR
design matrix
updated 8.0 years ago by
Aaron Lun
★ 28k • written 8.0 years ago by
kainblue100
• 0
1
vote
5
replies
951
views
DESEQ2 design matrix for double knockout interaction with condition
deseq2
model.matrix
design matrix
updated 5.0 years ago by
Michael Love
42k • written 5.0 years ago by
user9635643
• 0
7
votes
5
replies
1.6k
views
Which is the best design matrix to compare a timecourse between two conditions?
limma
voom
design matrix
timecourse
updated 7.3 years ago by
Gordon Smyth
50k • written 7.3 years ago by
Camila Orellana
▴ 10
4
votes
4
replies
1.7k
views
Is this design matrix correct (treament factor with three levels)?
edger
limma
design matrix
8.2 years ago
Ekarl2
▴ 80
4
votes
4
replies
1.7k
views
edgeR: How can I determine which factors significantly explain my DE genes?
edger
differential gene expression
model.matrix
design matrix
updated 6.0 years ago by
Gordon Smyth
50k • written 6.0 years ago by
Robert Gilmore
• 0
6
votes
4
replies
2.6k
views
discrepance in DESeq2 results with different design structures
deseq2
differential expression
independent filtering
pre-filtering
design matrix
9.0 years ago
Assa Yeroslaviz
★ 1.5k
2
votes
4
replies
8.5k
views
Limma LmFit() giving error: Error in lm.fit(design, t(M)) : incompatible dimension
limma
differential expression
design matrix
error
eset
updated 8.8 years ago by
Aaron Lun
★ 28k • written 8.8 years ago by
Vani
▴ 20
1
vote
4
replies
825
views
EdgeR multiple factor design question
edger
design matrix
7.4 years ago
bioinformatics
▴ 10
3
votes
4
replies
4.9k
views
multiple comparison using limma
limma
design matrix
makecontrasts
multiple comparisons
7.3 years ago • updated 7.2 years ago
annabelle.congras
▴ 10
1
vote
4
replies
946
views
Deseq multifactor test design full rank error and how to determine one variable only effect.
deseq2
multiple factor design
design matrix
6.2 years ago
jaime.alvarez.benayas
• 0
0
votes
4
replies
1.6k
views
edgeR design matrix
edgeR
design and contrast matrix
design matrix
updated 6.1 years ago by
Gordon Smyth
50k • written 6.2 years ago by
cafelumiere12
▴ 20
1
vote
4
replies
1.6k
views
edgeR design matrix for 2 variables + batch effect
egdeR
design matrix
batch effect
updated 4.5 years ago by
Gordon Smyth
50k • written 4.5 years ago by
ahsindoomilup
• 0
2
votes
3
replies
1.3k
views
Design Matrix with paired and independent samples plus RUVg Spike-in normalization
edger
design matrix
paired samples
ruvseq
updated 5.7 years ago by
James W. MacDonald
65k • written 5.7 years ago by
ChrisM
• 0
1
vote
3
replies
927
views
Defining the design matrix
design matrix
7.8 years ago
humberto_munoz
• 0
0
votes
3
replies
894
views
DESeq2 accounting for two confounders with interaction in the design matrix
deseq2
design matrix
multi-confounders
linear models
rna-seq
updated 4.2 years ago by
Michael Love
42k • written 4.2 years ago by
mohamedrefaat.1.1992
• 0
0
votes
3
replies
1.3k
views
Question on DESeq2 nested model example
deseq2
design matrix
updated 8.5 years ago by
Michael Love
42k • written 8.5 years ago by
Guido Hooiveld
★ 3.9k
4
votes
3
replies
1.3k
views
What is the correct contrast matrix if one treatment must be compared to both of the other two treatments, but separately?
edger
limma
model.matrix
contrast matrix
design matrix
updated 7.8 years ago by
Gavin Kelly
▴ 680 • written 7.8 years ago by
Peter
• 0
0
votes
3
replies
698
views
Proper Design Matrix for Paired Samples where endpoint has multiple samples that pair to the same start sample
edger
design matrix
updated 8.0 years ago by
Aaron Lun
★ 28k • written 8.0 years ago by
laura
• 0
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Comment: More regions in union when increasing DBA$config$mergeOverlap
by
Ian D.
▴ 70
Nudging my question. I could do with confirmation before proceeding with my current projects. Thank you.
Comment: Extremely small p-values using Limma for proteomic data
by
Robert Castelo
★ 3.3k
From the point of view of visualization, I find handy the fact that raw p-values give more resolution than adjusted ones, which allows one …
Comment: deseq2 results
by
ATpoint
★ 4.1k
> the results are not satisfactory to me Well, what is your question? The DESeq2 vignette covers how to do analysis, and some diagnostics…
Comment: Extremely small p-values using Limma for proteomic data
by
ATpoint
★ 4.1k
It's not an implementation problem I am discussing (it's trivial obviously) -- more that for most purposes the y-axis metric should be the …
Comment: Extremely small p-values using Limma for proteomic data
by
Robert Castelo
★ 3.3k
For visualization purposes you can draw an horizontal line at the p-value cutoff that meets the experiment-wide FDR cutoff. For instance, i…
Votes
Answer: minfi latest update for EPIC V2 chip
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