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0
votes
0
replies
98
views
flowDensity on a flowset
flowDensity
28 days ago
fredrikwallberg
• 0
0
votes
2
replies
252
views
Odd behavior of Rsubread::featureCounts when setting `nonOverlap = 0`
featureCounts
Rsubread
4 weeks ago • updated 13 days ago
isaac.vock
• 0
0
votes
0
replies
94
views
News:
Call for proposals: European CarpentryConnect - 12-14 November 2024, at EMBL Heidelberg
carpentry
conference
24 days ago
Wolfgang Huber
★ 13k
0
votes
4
replies
592
views
How to correct for known and surrogate variables in DESeq2?
DESeq2
BatchEffect
sva
limma
updated 16 days ago by
Michael Love
41k • written 6 months ago by
Crystal571
• 0
0
votes
1
reply
177
views
How can I create a GO plot with ggpubr and clusterProfiler libraries (begginer)
clusterProfiler
ggpubr
updated 24 days ago by
James W. MacDonald
65k • written 25 days ago by
BCS12
• 0
0
votes
0
replies
74
views
Exon-exon Junction Analysis for Variable-length Exons
Rsubread
8 days ago
Dario Strbenac
★ 1.5k
0
votes
0
replies
72
views
how to do Differential splicing from SGSeq analysis
DEXSeq
SGSeq
8 days ago • updated 3 days ago
Sara
• 0
0
votes
1
reply
118
views
what is the sampleFiles
chipseq
updated 16 days ago by
James W. MacDonald
65k • written 16 days ago by
zhulin
• 0
0
votes
0
replies
78
views
Enzyme Engineering
BioTIP
15 days ago
bocchemistry
• 0
0
votes
1
reply
139
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
updated 14 days ago by
ATpoint
★ 4.1k • written 15 days ago by
quanah.hudson
• 0
0
votes
5
replies
1.7k
views
blockwiseModule crashes in the end and doesn't generate modules
WGCNA
written 12 months ago by
mauricio.microbiol
• 0
0
votes
3
replies
1.2k
views
News:
European Bioconductor Conference 2023 planning
Europe
Conference
EuroBioC
Events
News
updated 10 days ago by
lauryfriese932
• 0 • written 18 months ago by
Maria Doyle
▴ 70
0
votes
3
replies
143
views
Error retrieving ensembl gene id using biomaRt...
biomaRt
updated 13 days ago by
James W. MacDonald
65k • written 13 days ago by
bas_work
▴ 30
0
votes
2
replies
238
views
featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_get_next_read_BIN: Assertion `l_name < 256' failed. Aborted (core dumpe…
metaMSdata
9 days ago • updated 7 days ago
shuwangxinze1996
• 0
0
votes
0
replies
65
views
News:
Interested in the analysis of Spatial Omics data?
tidySpatialExperiment
SpatialExperiment
MoleculeExperiment
9 days ago
info
▴ 10
0
votes
8
replies
323
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 7 days ago by
swbarnes2
★ 1.3k • written 9 days ago by
Aaliya
• 0
0
votes
1
reply
131
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 8 days ago by
Michael Love
41k • written 8 days ago by
kcarey
• 0
0
votes
2
replies
196
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
13 days ago • updated 10 days ago
catv
• 0
0
votes
18
replies
2.5k
views
problem installing ENmix package
ENmix
Bioconductor
rtracklayer
written 3.2 years ago by
lea
• 0
0
votes
0
replies
55
views
Could we detect difference at pathway level but not significant different at gene level?
GSVA
3 days ago
Chris
• 0
0
votes
1
reply
336
views
Gene names from Matrix
BioCor
StarBioTrek
MatrixGenerics
updated 22 days ago by
Lluís Revilla Sancho
▴ 730 • written 7 months ago by
karmasstark
• 0
0
votes
0
replies
15
views
How to create consensus peak sets for each group to use in DiffBind?
DiffBind
10 hours ago
Ian D.
▴ 70
0
votes
1
reply
114
views
ATACseq merge peaks
DiffBind
updated 6 days ago by
ATpoint
★ 4.1k • written 8 days ago by
Shloka
• 0
0
votes
1
reply
204
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots
HMMcopy
CopyNumberVariation
updated 2 days ago by
James W. MacDonald
65k • written 2 days ago by
SSSJec
• 0
0
votes
0
replies
55
views
Empty table plot using plotGseaTable()
fgsea
1 day ago
Chris
• 0
0
votes
0
replies
82
views
One error problem occurred caused by data type during using GDCprepare to handle BRCA data
GDCRNATools
TCGA
7 days ago
glaciya2018
• 0
0
votes
0
replies
142
views
Why output of GSEA is not the same but inversed, when you have the same but inversed input data?
clusterProfiler
gseKEGG
27 days ago
andromeda
• 0
0
votes
1
reply
86
views
putting my own expression data in deseq2
DESeq2
updated 2 days ago by
ATpoint
★ 4.1k • written 2 days ago by
Dev
• 0
0
votes
4
replies
187
views
FGSEA pre-ranked input in increasing order or decreasing
fgsea
14 days ago
sropri
• 0
0
votes
1
reply
98
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 1 day ago by
ATpoint
★ 4.1k • written 1 day ago by
angelathuynh5
• 0
0
votes
1
reply
133
views
Can you find the correct transcript in scRNA seq data
scRNAseq
RNAseq
updated 22 days ago by
ATpoint
★ 4.1k • written 24 days ago by
Merav
• 0
0
votes
1
reply
130
views
Bioinformatics student
Des
DESeq2
updated 20 days ago by
hemantcnaik
• 0 • written 20 days ago by
Sahar
• 0
0
votes
1
reply
148
views
Adding columns to DEXSeqResults object
DEXSeq
updated 14 days ago by
Alejandro Reyes
★ 1.9k • written 16 days ago by
Clyde
• 0
0
votes
0
replies
91
views
I am trying to detect alternative splicing.
ASpli
12 days ago
Sebashish
• 0
0
votes
1
reply
30
views
Gene-by-sample covariates in limma
Metabolomics
limma
updated 2 hours ago by
Gordon Smyth
50k • written 6 hours ago by
c53aba27
• 0
0
votes
3
replies
49
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
4 hours ago
arfranco
▴ 130
0
votes
3
replies
93
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 14 hours ago by
ATpoint
★ 4.1k • written 1 day ago by
james.zhang20
• 0
0
votes
7
replies
687
views
News:
List of Deprecated Packages for Bioc3.19
Release
Devel
ExperimentData
Software
9 weeks ago • updated 9 days ago
shepherl
3.9k
0
votes
3
replies
208
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
updated 2 days ago by
James W. MacDonald
65k • written 3 days ago by
SSSJec
• 0
0
votes
0
replies
88
views
Syntaxt Error in IsorcorrectoR package with Molecule Info File
Syntaxerror
IsoCorrectoR
16 days ago
Maliha Tabassum
• 0
0
votes
0
replies
81
views
News:
Important Release 3.18 Freeze Announcment
Release
release
Bioconductor
Bioconducto
20 days ago
shepherl
3.9k
0
votes
0
replies
85
views
queryDGIdb lexical error for some genes
lexicalerror
rDGIdb
DGIdb
json
22 days ago
Dennis
• 0
0
votes
2
replies
88
views
More regions in union when increasing DBA$config$mergeOverlap
DiffBind
1 day ago • updated 10 hours ago
Ian D.
▴ 70
0
votes
6
replies
330
views
unable to access Annotationhub latest record
DNAMethylation
AnnotationHub
EPICv2manifest
annotation
updated 22 days ago by
Tim Peters
▴ 170 • written 4 weeks ago by
s.malik
• 0
0
votes
2
replies
158
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
29 days ago
Marianna
▴ 20
0
votes
1
reply
106
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 14 days ago by
Michael Love
41k • written 14 days ago by
09191919
• 0
0
votes
1
reply
157
views
IgG control in RIP-seq with Deseq2
DESeq2
RIP-seq
updated 23 days ago by
Michael Love
41k • written 23 days ago by
Adelaide
• 0
0
votes
7
replies
292
views
Methylation Analysis experiment design using Limma
DNAMethylation
limma
methylationArrayAnalysis
15 days ago • updated 9 days ago
hortense96
• 0
0
votes
1
reply
235
views
CombineArrays for EPIC and EPIC V2
EPICV1
Methylation
combineArrays
minifi
EPICv2manifest
updated 28 days ago by
Tim Peters
▴ 170 • written 29 days ago by
Kim
• 0
0
votes
1
reply
89
views
Why does GSEA on edgeR results for randomized samples give highly significant p-values?
fgsea
edgeR
updated just now by
Gordon Smyth
50k • written 6 hours ago by
gene_bioconductor
▴ 10
206 results • Page
3 of 5
Recent ...
Replies
Comment: Deseq2 and Normalization of RNA sequencing data
by
kcarey
• 0
Noise in terms of expression...to make sure that the information reported is not due to artifacts and actually expression. When I had FPKM …
Answer: NEED FOR NESSASORY HELP
by
Gordon Smyth
50k
Is this quesion related to your earlier question? https://support.bioconductor.org/p/9157127/ If you've already done an edgeR analysis, yo…
Answer: Gene-by-sample covariates in limma
by
Gordon Smyth
50k
Your first method would be valid if, instead of taking ratios, you instead used the differences between the log-transformed 13C and 12C int…
Answer: Why does GSEA on edgeR results for randomized samples give highly significant p-
by
Gordon Smyth
50k
You have discovered something that I have pointed out many times on this forum. fgsea does not implement the classic GSEA method published …
Comment: Once again a "Model matrix not full rank"
by
James W. MacDonald
65k
In that case both time and treatment are identical things, so choose one and go with it.
Votes
Bioconductor 3.19 is Released!
A: Error in DESeqDataSet : some values in assay are not integers
Bioconductor 3.19 is Released!
Bioconductor 3.19 is Released!
Answer: DEseq2 coefficient
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