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Inquiry on Using DESeq2 for Analyzing Differential Translation Efficiency
RiboSeq
DESeq2
written 17 months ago by
noi.cohen
• 0
1
vote
2
replies
528
views
Deseq2 - differenc in results
DESeq2
written 17 months ago by
rajan
• 0
3
votes
3
replies
1.0k
views
voom does not seem to properly correct for library size
limma
updated 17 months ago by
Gordon Smyth
53k • written 17 months ago by
ATpoint
★ 5.0k
1
vote
3
replies
651
views
Missing test data for RiboProfiling package
RiboProfiling
updated 17 months ago by
James W. MacDonald
68k • written 17 months ago by
max.ferretti
• 0
2
votes
8
replies
1.5k
views
AffylmGUI does not start wit R 4.4.1
affylmGUI
updated 16 months ago by
Gordon Smyth
53k • written 17 months ago by
richardallenfriedmanbrooklyn
▴ 30
0
votes
7
replies
1.2k
views
DESeq design with and without intercept is giving different results
DESeq2
written 17 months ago by
ktz
• 0
1
vote
5
replies
1.7k
views
RNASeq normlization for samples with global shift in gene expression
qsmooth
DESeq2
limma
Normalization
updated 17 months ago by
Gordon Smyth
53k • written 17 months ago by
Xuan Yi
• 0
1
vote
3
replies
1.7k
views
Non-zero exit status error when installing GenomeInfoDbData
DECIPHER
GenomeInfoDbData
Bioconductor
GenomeInfoDb
written 17 months ago by
dbjohnson7
• 0
0
votes
3
replies
1.0k
views
Merging multi-fastq samples in recount3
recount3
written 17 months ago by
Charlotte Capitanchik
• 0
0
votes
2
replies
1.4k
views
recount
recount
updated 17 months ago by
Leonardo Collado Torres
★ 1.1k • written 3.7 years ago by
nathan-temple
• 0
0
votes
3
replies
1.2k
views
More significant genes after lfcShrink in DEseq2?
DESeq2
written 17 months ago by
m.orlov
• 0
0
votes
0
replies
765
views
Job:
PhD position: Analysis of epistatic interactions in enzymes using long-read sequencing data
NGS
AI
Bioconductor
updated 17 months ago by
Tyler Sagendorf
▴ 10 • written 17 months ago by
Gerhard Thallinger
▴ 180
0
votes
0
replies
575
views
BioCAsia 2024 Abstract Deadline - August 26th
BioCAsia2024
Community
Conference
Bioconductor
17 months ago
Maria Doyle
▴ 110
0
votes
3
replies
1.0k
views
Differential expression analysis in time course experiments with DESeq2
DESeq2
limma
DifferentialExpression
updated 17 months ago by
Gordon Smyth
53k • written 17 months ago by
Jiahao Tian
• 0
0
votes
1
reply
496
views
CleanUpRNAseq package
CleanUpRNAseq
updated 17 months ago by
Haibo Liu
▴ 20 • written 17 months ago by
mrhm12r
• 0
0
votes
3
replies
819
views
Create TidySummarizedExperiment from RangedSummarizedExperiment?
tidyomics
written 17 months ago by
Ariel
• 0
0
votes
3
replies
1.5k
views
flowAI: flow rare check in R vs FlowJo
flowAI
updated 17 months ago by
mongianni1
• 0 • written 23 months ago by
Olena
• 0
0
votes
2
replies
732
views
Analysis of transcription inhibition with DESeq2
SpikeIn
DESeq2
Normalization
written 17 months ago by
ADopico
• 0
2
votes
4
replies
1.7k
views
# Invalid keytype: GOALL. Please use the keytypes method to see a listing of valid arguments.
clusterProfiler
makeOrgPackage
gseGO
updated 17 months ago by
James W. MacDonald
68k • written 17 months ago by
Nuria Mauri
• 0
6
votes
5
replies
4.7k
views
What is the importance of `--countReadPairs` in featureCounts on paired end data?
featureCounts
DESeq2
updated 12 months ago by
rohitsatyam102
▴ 20 • written 17 months ago by
Bioinformagician
• 0
0
votes
1
reply
773
views
DiffBind - observation that the default union overlap is actually a gap of 1 bp
DiffBind
17 months ago • updated 11 months ago
Ian D.
▴ 70
0
votes
1
reply
1.0k
views
installing "org.Hs.eg.db",go error
org.Hs.eg.db
updated 17 months ago by
shepherl
4.3k • written 17 months ago by
聪
• 0
4
votes
5
replies
13k
views
[sva] Error in density.default(x, adjust = adj) : 'x' contains missing values
sva
updated 17 months ago by
ndphuc1605
• 0 • written 8.3 years ago by
anton.kratz
▴ 60
3
votes
4
replies
1.4k
views
using limma trend workflow in CAMERA
CAMERA
limma
17 months ago
Jiahao Tian
• 0
0
votes
0
replies
531
views
Are equal whisker lengths important in an RLE plot?
EDASeq
EDA
17 months ago
BioinfGuru
▴ 70
0
votes
0
replies
775
views
News:
EuroBioC Updates: Important Deadlines
Conference
EuroBioC2024
Bioconductor
Community
EuroBioC
17 months ago
Maria Doyle
▴ 110
3
votes
3
replies
1.8k
views
the count matrix have decimal values
DESeq2
DifferentialExpression
updated 17 months ago by
James W. MacDonald
68k • written 17 months ago by
Ibrahim
• 0
0
votes
2
replies
1.3k
views
R is Removing the Gene Names in My Count Data
DESeq2
updated 17 months ago by
BioinfGuru
▴ 70 • written 17 months ago by
thatolivebranch
• 0
1
vote
4
replies
1.6k
views
Recommendations for DESeq2 Independent Filtering with Pseudobulk Data
DESeq2
independentfiltering
glmGamPoi
17 months ago
Bob
▴ 30
0
votes
1
reply
655
views
Using DESeq2 for Analyzing AMR Gene Variations in Water Samples Post-Treatment
DESeq2
NanoporeRNASeq
DNASeq
updated 17 months ago by
Michael Love
43k • written 17 months ago by
Dot
• 0
0
votes
2
replies
1.1k
views
DMPs enrichment for MOUSE DNA methylation array
minfi
mCSEA
missme
missMethyl
updated 17 months ago by
nrenke
• 0 • written 17 months ago by
Lucie
• 0
3
votes
3
replies
1.1k
views
One-sided tests with CAMERA-PR
CAMERA
limma
updated 17 months ago by
Gordon Smyth
53k • written 17 months ago by
Tyler Sagendorf
▴ 10
0
votes
2
replies
1.0k
views
FlowSOM library requires igraph but igraph does not install
FlowSOM
updated 9 months ago by
Szabolcs
• 0 • written 17 months ago by
Priyanka
• 0
0
votes
2
replies
547
views
get_snp
frmaTools
updated 17 months ago by
James W. MacDonald
68k • written 17 months ago by
Hans
• 0
0
votes
0
replies
616
views
hichip fragments extraction chip seq
ChIPSeqData
interact
GenomicRanges
HiC
17 months ago
user_bioinfo
• 0
0
votes
3
replies
1.2k
views
comparing common differentially expressed genes from two Rna-Seq Dataset.
DESeq2
edgeR
DifferentialExpression
written 17 months ago by
dancingcow
• 0
3
votes
5
replies
2.6k
views
DESeq2: How to Compare the Condition Effects of Two Different Treatments?
DESeq2
RNAseq
DifferentialExpression
updated 17 months ago by
swbarnes2
★ 1.4k • written 17 months ago by
BioNovice247
• 0
2
votes
1
reply
1.8k
views
DESeq2 is it OK to scale transformed data in order to plot heatmaps?
DESeq2
heatmaps
updated 17 months ago by
ATpoint
★ 5.0k • written 17 months ago by
jeba95
• 0
0
votes
1
reply
672
views
DiffBind remove Unplace Chromosome (chrUn)
DiffBind
17 months ago
mdidish
▴ 10
0
votes
0
replies
563
views
DiffBind dba.plotprofile y-axis range
DiffBind
17 months ago
Tara
• 0
2
votes
4
replies
892
views
Bioconductor Submission question
Bioconductor
17 months ago
XFWu
• 0
2
votes
3
replies
1.4k
views
RUV - Does my data need further normalisation?
EDASeq
plotRLE
RNAseq
17 months ago
BioinfGuru
▴ 70
2
votes
2
replies
945
views
Why is the inclusion of RUVs not reflected in the EDA plots produced from normalised counts extracted from deseq2?
RUVSeq
DESeq2
dese
17 months ago
BioinfGuru
▴ 70
0
votes
0
replies
498
views
proActiv-promoter analysis
proActiv
Transcriptomics
17 months ago
wang.xiangyu
• 0
2
votes
1
reply
2.6k
views
Expected memory usage for analyzing large single-cell seq datasets (> 450,000 cells)?
BigData
Clustering
memory
SingleCellExperiment
edgeR
updated 17 months ago by
Aaron Lun
★ 29k • written 17 months ago by
Joseph
• 0
0
votes
0
replies
593
views
News:
Annual Nominations Open for CAB and TAB! Deadline Aug 31
TAB
CAB
Bioconductor
17 months ago
shepherl
4.3k
1
vote
2
replies
837
views
Combining DESeq2 and Within-Group Variability to Identify Stochastic Genes
DESeq2
17 months ago
DL
• 0
0
votes
3
replies
759
views
How to combine multiple lists of cytoframes/cytocq data objects into one
flowWorkspace
cytocq
updated 17 months ago by
SamGG
▴ 360 • written 17 months ago by
anders.tondell
▴ 30
0
votes
1
reply
875
views
edgeR - design a complex matrix
design
model.matrix
edgeR
complexdesign
updated 17 months ago by
Gordon Smyth
53k • written 17 months ago by
Karla
• 0
1
vote
4
replies
1.6k
views
How to Query Only SNPs from the biomaRt Variation Database
biomaRt
17 months ago
kschecht
• 0
1,000 results • Page
18 of 20
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Replies
Answer: Array weights in dpcDE
by
Gordon Smyth
53k
The uncertainty of quantification is fully accounted for in the dpcDE() analysis, including in the computation of sample weights. Your use…
Answer: lfc shrinkage estimator in DESeq2
by
Michael Love
43k
Try the vignette which has a lot of information on these type of questions.
Comment: limpa analysis advice
by
Gordon Smyth
53k
The DPC slope is beta1. The estimated slopes for your data look pretty low, lower than I've ever used for a real dataset. Nevertheless, as …
Comment: limpa analysis advice
by
SamGG
▴ 360
Thanks for your feedback and for reassuring me. :-) As you suggested, I tried dpcCN() and set the slope to 0.7 in addition to the standard…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
chp265
• 0
Good point, I hadn't considered the fact quantitative `time` implies a (logit-)linear response *to* time. You're correct that I haven't exp…
Votes
A: DESeq2: Is it possible to convert read counts to expression values via TPM and r
A: Filtering read counts matrix: how to deal with duplicated gene symbols, differen
Comment: limpa analysis advice
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
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