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Showing :
Limma
•
reset
15
votes
22
replies
1.8k
views
The difference when using Limma compare between 3 conditions vs 2 conditions
limma
27 days ago • updated 5 days ago
Chris
• 0
12
votes
12
replies
781
views
Why p value inconsistent when using different number of pathway score?
limma
updated 4 days ago by
Robert Castelo
★ 3.3k • written 5 days ago by
Chris
• 0
11
votes
4
replies
1.8k
views
LIMMA dupcor() function time issue.
limma
microarray
block
updated 7 months ago by
Gordon Smyth
50k • written 5.2 years ago by
tleona3
• 0
9
votes
5
replies
5.1k
views
Differential expression analysis: Permutation Test
deseq2
limma
edger
permutation
updated 5 months ago by
Gordon Smyth
50k • written 8.2 years ago by
Radek
▴ 90
8
votes
6
replies
428
views
DGE analysis with LIMMA
limma
updated 7 weeks ago by
Gordon Smyth
50k • written 7 weeks ago by
Amit
• 0
7
votes
6
replies
434
views
Batch Effect Correction, Gene Filtering
DGE
limma
updated 4 weeks ago by
Gordon Smyth
50k • written 4 weeks ago by
Amit
• 0
7
votes
2
replies
771
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR
limma
voom
RNASeq
muscat
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
annikagable
• 0
6
votes
5
replies
6.1k
views
Using Limma R package For Proteomics differential expression
limma
ProteomicsWorkflow
updated 7 months ago by
Gordon Smyth
50k • written 2.5 years ago by
David Lyon
▴ 340
6
votes
7
replies
750
views
Adding a group of samples reduces DEGs in other groups
RNASeq
limma
updated 8 months ago by
Gordon Smyth
50k • written 8 months ago by
Jonathan
▴ 10
5
votes
8
replies
8.8k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 3 days ago by
hortense96
• 0 • written 7.7 years ago by
dmcleanuk
▴ 30
5
votes
25
replies
826
views
limma models out of memory issue on Slurm HPC
Regression
limma
STATISITICS
HPC
updated 21 days ago by
Gordon Smyth
50k • written 4 weeks ago by
Jitendra
▴ 10
5
votes
7
replies
430
views
DEG Filtering
MicroarrayData
limma
Genefiltering
updated 29 days ago by
Gordon Smyth
50k • written 5 weeks ago by
Amit
• 0
5
votes
2
replies
673
views
PhD student
TMM
limma
9 months ago
Nisha
• 0
5
votes
3
replies
692
views
limma: More differentially expressed genes when factor included in model than when used as blocking factor
voom
blocking
limma
updated 6 months ago by
Gordon Smyth
50k • written 6 months ago by
Gerhard Thallinger
▴ 180
5
votes
9
replies
860
views
Light difference when using coef vs omit a group when compare 3 groups.
limma
15 days ago • updated 7 days ago
Chris
• 0
5
votes
2
replies
536
views
Filter genes by expression for voomLmFit
DEG
edgeR
limma
voom
filterByExpr
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
annikagable
• 0
5
votes
6
replies
3.7k
views
Differential protein/biomarker expression using limma: is that possible?
Proteomics
NPX
limma
updated 3 months ago by
briankleiboeker
• 0 • written 3.1 years ago by
r.i.s.alnuwaysir
▴ 10
5
votes
9
replies
1.2k
views
Batch correction and repeated measures
RUVSeq
sva
limma
updated 8 days ago by
Gordon Smyth
50k • written 7 months ago by
Jonathan
▴ 10
5
votes
8
replies
1.4k
views
Can the limma package be applied to Log2 RUV-normalized data?
RUVnormalizeData
MicroarrayData
limma
updated 4 months ago by
Shaimaa Gamal
• 0 • written 11 months ago by
pg45863
▴ 10
5
votes
5
replies
780
views
limma output for epic array methylation data
linearregression
epicarray
MethylationArray
limma
8 months ago
Jitendra
▴ 10
4
votes
6
replies
558
views
filtering lowly expressed reads
RNASeq
limma
DifferentialExpression
Preprocessing
updated 11 weeks ago by
Gordon Smyth
50k • written 3 months ago by
Shaimaa Gamal
• 0
4
votes
8
replies
2.1k
views
Can we use rlog or vst data values in limma for differential expression analysis?
DESeq2
limma
Normalization
R
updated 11 months ago by
Gordon Smyth
50k • written 17 months ago by
Sabiha
▴ 20
4
votes
2
replies
1.2k
views
Gene set analysis: GSVA, Z-Score, and fold-changes
GSVA
RNASeq
limma
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
Jonathan
▴ 10
4
votes
3
replies
334
views
voom with quality weights after RUV correction
RUVSeq
limma
DifferentialExpression
updated 11 weeks ago by
Gordon Smyth
50k • written 11 weeks ago by
Shaimaa Gamal
• 0
4
votes
5
replies
859
views
Phenotype effect - building a contrast matrix - grouping vs interaction
limma
updated 7 months ago by
Gordon Smyth
50k • written 7 months ago by
Jonathan
▴ 10
4
votes
7
replies
2.0k
views
should statmod be a dependency of limma and not a suggests?
limma
updated 8 months ago by
Gordon Smyth
50k • written 7.9 years ago by
nathan
• 0
3
votes
3
replies
561
views
comparing contrasts in edger/lima using makeContrasts function
DifferentialExpression
edgeR
limma
updated 7 months ago by
James W. MacDonald
65k • written 7 months ago by
stuartthafass
• 0
3
votes
5
replies
861
views
limma-trend vs. limma-voom theoretical difference
limma
limma-trend
limma-voom
updated 3 months ago by
floriandeckert
▴ 30 • written 17 months ago by
hainct
• 0
3
votes
2
replies
438
views
For limma, if I leave coef=NULL, which test is the P value for?
limma
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
S
• 0
3
votes
11
replies
2.4k
views
Adjusting for baseline when having longitudinal data, mixed model and no treatment
RNAseq
mixedmodels
baselineadjustement
limma
11 months ago • updated 10 months ago
Anna
▴ 20
3
votes
3
replies
1.2k
views
Differential expression analysis with only FPKM matrix available from total newbie in R
FPKM
RNASeqData
DEAnalysis
limma
7 months ago
Ethan Nguyen
• 0
3
votes
2
replies
275
views
Design matrix after RUVg correction
RUVSeq
limma
DifferentialExpression
11 weeks ago
Shaimaa Gamal
• 0
3
votes
1
reply
120
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 7 days ago by
Gordon Smyth
50k • written 8 days ago by
Shaimaa Gamal
• 0
3
votes
13
replies
1.3k
views
Timely differentially expressed genes
timecoursedata
limma
7 months ago • updated 4 months ago
triZZla
• 0
3
votes
4
replies
519
views
Is it appropriate to apply linear mixed models to Voom transformed data?
stats
r
RNASeqData
limma
voom
5 months ago
pl23
• 0
3
votes
11
replies
1.3k
views
SD filtering before DEG analysis with limma : possible or not ?
filtering
STD
DEG
limma
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
alexia.brunel
• 0
3
votes
3
replies
913
views
how to remove batch effects of RNA seq data based on continuous variables(like surrogate variables from sva package) using combatseq or limma (remove…
combatseq
RNASeqData
sva
limma
10 months ago
amnahsiddiqa
▴ 10
3
votes
5
replies
605
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 7 weeks ago by
Michael Love
41k • written 8 weeks ago by
sap275
▴ 10
3
votes
8
replies
482
views
Constrast for Time Course Experiments using Limma
limma
7 weeks ago
dl17032000
• 0
3
votes
4
replies
701
views
Anova table edgeR limma
limma
RNASeq
edgeR
11 months ago
KATHERINE CHUERE NUNES
• 0
3
votes
6
replies
832
views
Question on constructing design matrix and defining contrasts for analysis
wgcna
DESeq2
edgeR
limma
design
updated 11 months ago by
Gordon Smyth
50k • written 11 months ago by
mohammedtoufiq91
▴ 10
2
votes
4
replies
1.2k
views
RNAseq : identical sample-sample distances after using limma::removeBatchEffect
DESeq2
RNASeq
BatchEffect
limma
updated 7 months ago by
Michael Love
41k • written 2.9 years ago by
athiebaut
• 0
2
votes
4
replies
613
views
Correct the batch effect in methylation analysis in R
BatchEffect
limma
methylationArrayAnalysis
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
kyj2226
• 0
2
votes
1
reply
246
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 8 weeks ago by
Michael Love
41k • written 8 weeks ago by
sap275
▴ 10
2
votes
3
replies
1.1k
views
bulk RNA-seq analysis using R : batch effect : One question concerning Combat-seq and one question concerning DESeq2 design = batch + condition
BatchEffect
sva
DESeq2
RNASeqData
limma
updated 8 months ago by
James W. MacDonald
65k • written 8 months ago by
D-Bug
▴ 10
2
votes
5
replies
1.3k
views
removing the batch effect for vst counts and defining the batches in removeBatchEffect function
DESeq2
BatchEffect
limma
updated 8 months ago by
Gordon Smyth
50k • written 8 months ago by
Sara
• 0
2
votes
3
replies
353
views
TMM normalization after RUVg correction
RUVSeq
limma
DifferentialExpression
edgeR
Normalization
updated 11 weeks ago by
James W. MacDonald
65k • written 11 weeks ago by
Shaimaa Gamal
• 0
2
votes
1
reply
289
views
removeBatchEffect with non-linear model fit
limma
DESeq2
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
georgii.vdovin
• 0
2
votes
8
replies
497
views
Microarray Limma Model fitting and DEG
limma
DGEanalysis
updated 29 days ago by
Gordon Smyth
50k • written 4 weeks ago by
Amit
• 0
2
votes
4
replies
591
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom
RNASeq
limma
5 months ago
Tyler Sagendorf
▴ 10
50 results • Page
1 of 1
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Comment: Deseq2. decontXcounts not integers. Useful alternative?
by
ATpoint
★ 4.0k
I do not know this decontamination tool you use but generally: If these counts are basically "corrected" raw counts in the sense that they'…
Answer: Improving the design matrices vignette in RNAseq123
by
Gordon Smyth
50k
Thanks to Shian Su, we have confirmed that the problem in the design matrices vignette html is caused by a bug in knitr 1.44 (to do with sp…
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
by
winwater0928
• 0
Thank you for your comment! I'll review my code from top to bottom.
Comment: Math expression in shapeCustom legend in EnhancedVolcano
by
James W. MacDonald
65k
So basically you are using HTML markup to set your subscripts and whatnot, and then `element_markdown` does the rest.
Comment: Math expression in shapeCustom legend in EnhancedVolcano
by
James W. MacDonald
65k
Oh snap. Foiled again. Here it is with spaces & ge ; and & lt ;
Votes
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
biomaRt error: database disk image is malformed
Comment: biomaRt : No lock file for BiocFileCache
Answer: Cross-species analysis - how to compute the mid parent value?
Answer: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
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