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NanoString
•
reset
0
votes
0
replies
1.1k
views
Trying to use a NanoString expression set with batch effects to do GSVA
GSVA
Normalization
BatchEffect
GSVAdata
NanoString
23 months ago
Zhijie
• 0
0
votes
3
replies
2.7k
views
[ExpressionSet] Question: Error in validObject(.Object)
ExpressionSet
Assay
NanoString
Error
Data
updated 2.4 years ago by
James W. MacDonald
67k • written 2.4 years ago by
junli1988
• 0
2
votes
3
replies
2.0k
views
Housekeeping genes vary across contrast groups, using DESeq2 on NanoString data
NanoNormIter
DifferentialExpression
Housekeeping
DESeq2
NanoString
updated 2.9 years ago by
Michael Love
43k • written 2.9 years ago by
argonvibio
• 0
1
vote
5
replies
2.1k
views
how to plot multiple RLEs on same plot?
plotRLE
Nanostring
RUVSeq
DESEQ2
updated 4 months ago by
BioinfGuru
▴ 70 • written 3.5 years ago by
xiaofeiwang18266
▴ 50
3
votes
6
replies
2.2k
views
Why NanoStringDiff package so slow?
NanoStringDiff
NanoString
nCounter
DE analysis
updated 13 months ago by
georgersmith
• 0 • written 5.4 years ago by
lim6432
▴ 50
0
votes
1
reply
1.9k
views
Application of RUV to a small Nanostring dataset
RUV
Nanostring
RUV-III
updated 4.7 years ago by
hermidalc
▴ 20 • written 5.4 years ago by
raf4
▴ 30
0
votes
9
replies
1.8k
views
DESeq2 with nanostring data
deseq2
nanostring
5.0 years ago
acs1990
▴ 10
0
votes
4
replies
1.2k
views
Setting up contrasts with 'limma', patient data, small number of repeats
limma
nanostring
contrasts
updated 5.2 years ago by
Gordon Smyth
52k • written 5.2 years ago by
uridavid.akavia
• 0
16
votes
40
replies
10k
views
Can NanoString data be analyzed using DESeq2?
NanoString
Differential Expressed Genes Analysis
DESeq2
updated 3.0 years ago by
Clara
▴ 10 • written 5.4 years ago by
lim6432
▴ 50
1
vote
1
reply
1.7k
views
GSVA with NanoString nCounter data
GSVA
Nanostring
6.4 years ago • updated 5.7 years ago
SB
• 0
0
votes
5
replies
1.5k
views
NanoStringDiff analysis with confounding factors
differential gene expression
nanostring
confounding factors
updated 6.0 years ago by
James W. MacDonald
67k • written 6.0 years ago by
Guillaume Robert
• 0
1
vote
6
replies
2.3k
views
How to access normalized data in the NanoStringDiff package?
nanostringdiff
nanostring
NanoStringDiff
updated 6.5 years ago by
James W. MacDonald
67k • written 6.5 years ago by
casey.rimland
▴ 170
9
votes
13
replies
6.3k
views
DESeq2 on NanoString Data
deseq2
nanostring
updated 6.5 years ago by
Michael Love
43k • written 6.5 years ago by
casey.rimland
▴ 170
1
vote
1
reply
1.2k
views
DESeq2 confounding cartridge
deseq2
confounders
nanostring
rnaseq
differential gene expression
updated 6.7 years ago by
Michael Love
43k • written 6.7 years ago by
kim.malek88
• 0
0
votes
0
replies
1.5k
views
design matrix and contrast for paired experiment using NanoStringDiff for nCounter data
nanostring
NanoStringDiff
design and contrast matrix
differential gene expression
ncounter
7.4 years ago
c.kohler
• 0
3
votes
8
replies
3.8k
views
Using DESeq2 with Nanostring data (for VST only)
deseq2
variancestabilizingtransformation
nanostring
updated 7.7 years ago by
Michael Love
43k • written 7.7 years ago by
johnmcma
▴ 10
2
votes
0
replies
2.1k
views
NanoString Data Normalization Revisited
nanostring
normalization
9.2 years ago
alakatos
▴ 130
24
votes
12
replies
13k
views
Nanostring analysis with limma
nanostring
limma
updated 9.6 years ago by
ker61
▴ 20 • written 9.7 years ago by
mali salmon
▴ 370
18 results • Page
1 of 1
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Replies
Answer: Cross-validation with multiple control subgroups in limma
by
Gordon Smyth
52k
limma doesn't do cross-validation or resampling. Like most classical linear modeling procedures in statistics, limma uses a statistical mod…
Comment: Issues with seqlevelsStyle when making custom txdb objects for genomes/annotatio
by
Robert Castelo
★ 3.4k
Hi Hervé, thank you very much for your input into how to properly build and manage TxDb objects. gDNAx has two main inputs, one or more BAM…
Comment: Biostrings: Error writing long reads (>200 kbps) with writeQualityScaledXStringS
by
Aidan
▴ 60
Quick update: this is fixed in a branch awaiting PR to Biostrings (https://github.com/Bioconductor/Biostrings/pull/122). Should be in `deve…
Answer: Can DESeq2's design compensate for sequencing experimental design shortcomings?
by
Michael Love
43k
For statistical analysis plans, I recommend working with a local statistician or someone familiar with linear models in R. I have to reserv…
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
xiaofeiwang198266
• 0
Thanks for your reply!
Votes
C: edgeR and lack of counts ID on CPM matrix
A: DESeq2 Following RSEM
A: importing RSEM data into DESeq2
A: Expected counts from RSEM in DESeq2
A: Using RSEM reads for DESeq2
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