Showing : Normalization • reset
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981
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gene detection across experiments
DESeq2 Normalization
5 months ago Mara • 0
0
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3
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1.7k
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RNA-seq: TPM batch effect correction
rnaseqDTU Normalization
updated 5 months ago by 68k • written 5 months ago by • 0
0
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5
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885
views
WGCNA
WGCNA Normalization RNASeq
written 9 months ago by • 0
0
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2
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527
views
Meffil: error in functional normalization function (Error in rg.to.mu(rg, probes))
methylationArrayAnalysis Normalization Epigenetics MethylationArrayData
10 months ago a.alkema • 0
6
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5
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1.1k
views
How do I fix an odd voom plot in a combined dataset?
limma Normalization voom
updated 10 months ago by 68k • written 11 months ago by • 0
0
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0
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370
views
Lipidomics Barplot: raw values or non-log, lipidr-normalized values?
Normalization Lipidomics lipidr
12 months ago David R ▴ 90
3
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4
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1.2k
views
Limma- Print-tip loess normalisation-printer layout information does not match M row dimension
Normalization limma within-arraynormalisation Printerlayout
12 months ago dseauer • 0
1
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2
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1.4k
views
Consistent Decimals In Data After Antibody Array Background Correction Using Limma:nec Function
limma MicroarrayData Normalization
13 months ago Ai • 0
0
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0
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590
views
Diffbind: Cut&Run: E-coli spike-in
Normalization DiffBind
13 months ago kim • 0
0
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1
reply
932
views
Spike-in normalization method in ATACseq
sperm Drosophila_melanogaster ATACSeq Normalization SpikeIn
updated 13 months ago by ★ 5.0k • written 13 months ago by • 0
3
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4
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1.3k
views
Are transformed values from rlog /vst log2 normalized counts?
Normalization DESeq2 rlog vst
13 months ago Eva ▴ 10
0
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0
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617
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Background normalization of Diffbind dba.normalize
Normalization DiffBind
13 months ago Young Boqin • 0
1
vote
1
reply
694
views
Normalization RNA-seq for multiple plates
Normalization DESeq2 RNASeqData edgeR
updated 14 months ago by ★ 5.0k • written 14 months ago by • 0
1
vote
5
replies
1.5k
views
RNASeq normlization for samples with global shift in gene expression
qsmooth DESeq2 limma Normalization
updated 15 months ago by 53k • written 15 months ago by • 0
0
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2
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684
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Analysis of transcription inhibition with DESeq2
SpikeIn DESeq2 Normalization
written 15 months ago by • 0
1
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3
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1.9k
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Combining two bulk RNA seq datasets
RNASeqData DESeq2 Normalization
17 months ago Tanvi • 0
2
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2
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882
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Control group between 2 batches have different expression in RNAseq data
BatchQC BatchEffect rnaseqGene Normalization
updated 18 months ago by ▴ 10 • written 18 months ago by • 0
0
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2
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975
views
Normalisation using clinical traits in RNA seq using DESeq2
DESeq2 clinicaltraits Normalization batchnormalisation
18 months ago sukeshinik5 ▴ 20
0
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1
reply
765
views
normalization across different species for DESeq2 analysis
DESeq2 normalization Normalization acrossspecies
updated 18 months ago by 43k • written 18 months ago by • 0
0
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1
reply
722
views
normalization using Deseq2
DESeq2 rnaseqGene Normalization
updated 19 months ago by ★ 5.0k • written 19 months ago by • 0
2
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2
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1.8k
views
[Diffbind] Blacklist error: Error: Invalid Normalization and Unable to apply Blacklist/Greylist
DiffBind Normalization
20 months ago • updated 19 months ago Henry ▴ 10
1
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6
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1.6k
views
DESeq2 step-by-step Normalization then DGE separately & Normalization methods and switchability
DESeq2 Normalization
updated 20 months ago by 43k • written 20 months ago by • 0
0
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2
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938
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edgeR analysis: glm functions and offset matrix
tximport edgeR Normalization
20 months ago Marianna ▴ 20
1
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4
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1.1k
views
EDAseq to normalise for both gene-length and GC-content
norma EDASeq EDA Normalization
20 months ago samuel.channon • 0
0
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0
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615
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TCGAbiolinks STAD project 450k Methylation Beta Value data
MethylationArray normalize450K TCGAbiolinks Normalization MethylationArrayData
21 months ago • updated 20 months ago Giovanni Calice ▴ 160
2
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1
reply
799
views
Normalization factors when there is a possible unidirectional expression
limma Normalization DifferentialExpression
updated 21 months ago by ★ 5.0k • written 21 months ago by • 0
2
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2
replies
1.7k
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2 Normalization RNASeq
22 months ago jackafiore • 0
2
votes
3
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1.1k
views
TMM normalization after RUVg correction
RUVSeq limma DifferentialExpression edgeR Normalization
updated 22 months ago by 68k • written 22 months ago by • 0
1
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3
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1.3k
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics Normalization RNASeq DESeq2
written 22 months ago by • 0
0
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1
reply
1.2k
views
WGCNA Soft Power
Normalization DESeq2 Network RNASeqData
updated 22 months ago by ★ 5.0k • written 22 months ago by • 0
2
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4
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2.2k
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization edgeR RNASeq
22 months ago Sabiha ▴ 20
1
vote
1
reply
1.0k
views
DESeq2 Normalization for QC Samples
DESeq2 Normalization
updated 23 months ago by 43k • written 23 months ago by • 0
2
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2
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3.6k
views
DESeq2 median of ratios normalization instead of VST or rlog for ordinations?
DESeq2 medianofratiostransformation StatisticalMethod Normalization dispersion
23 months ago Linton • 0
0
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0
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812
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SomaticSignatures normalization for trinucleotide motifs in reference genome
Normalization SomaticSignatures
2.0 years ago vasily.dobrovolsky • 0
0
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6
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2.3k
views
DESeq2 Normalization with 4 Groups
Normalization RNASeq DESeq2
2.1 years ago erika.maldonado-rosado • 0
0
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0
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730
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Dual species RNA-seq, Library size and normalization
Normalization edgeR RNASeq DESeq2
2.1 years ago Florian • 0
1
vote
11
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2.9k
views
DESeq2 gene length normalisation
Normalization DESeq2
updated 2.1 years ago by 43k • written 2.1 years ago by • 0
1
vote
4
replies
6.0k
views
How to process Nimblegen microarray .pair files to get normalized expression matrix
Microarray Preprocessing NimbleGen Normalization
written 3.0 years ago by • 0
1
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5
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2.0k
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI normalization limma voom Normalization
updated 2.4 years ago by 53k • written 2.4 years ago by • 0
0
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3
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1.1k
views
Normalization of different input materials
Normalization DESeq2
updated 2.4 years ago by ★ 5.0k • written 2.4 years ago by ▴ 10
0
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2
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1.5k
views
Can VST-transformed community composition data be expressed as relative abundance?
vst relativeabundance Normalization DESeq2
2.5 years ago dwas • 0
5
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11
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7.5k
views
Visualization of negative log CPM values
Visualization Normalization ggplot2 edgeR
written 2.5 years ago by ▴ 20
0
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3
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1.3k
views
Is the gene-specific PCR efficiency a serious concern for intrasample comparisons in RNA-Seq?
RNA-Seq normalization PCR_efficiency bias intrasample_comparison
updated 2.6 years ago by 68k • written 2.6 years ago by • 0
1
vote
2
replies
1.7k
views
Filter by expression and keep probes in limma
R filter limma Normalization Microarray
2.6 years ago Abdul ▴ 20
2
votes
2
replies
1.2k
views
Question about transforming log expression values to antilog values in limma
IlluminaChip limma AffymetrixChip Normalization Microarray
2.7 years ago Abdul ▴ 20
1
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2
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1.0k
views
RNA-Seq normalization of pre and post treatment groups
Normalization RNASeq
written 2.7 years ago by • 0
0
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2
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1.6k
views
Scaling and normalization by deconvolution of SMART-Seq 2 data
scran Clustering SingleCell Normalization
2.7 years ago Assa Yeroslaviz ★ 1.5k
1
vote
6
replies
3.0k
views
can negative value microarray matrix data analysed by limma directly
limma microarray normalization
updated 2.3 years ago by 53k • written 5.2 years ago by ▴ 20
2
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3
replies
3.7k
views
deseq2 normalization and statistical method
normalization deseq2 StatisticalMethod
updated 2.7 years ago by ★ 5.0k • written 2.7 years ago by ▴ 10
2
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1
reply
2.0k
views
deseq2 RLE normalization
RLE normalization deseq2
updated 2.7 years ago by ★ 5.0k • written 2.7 years ago by ▴ 10
1,761 results • Page 1 of 36
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Answer: problem reading bam files into nucleR by James W. MacDonald 68k
The first step is to update to the current versions of R/Bioconductor and try again. It's possible that you have run into a bug that's alre…
Answer: Question about DESeq2 Design for Paired Treatment Study by ATpoint ★ 5.0k
It's a paired analysis so you don't need any patient-specific covariates such as age and sex. What you call batch effect might just be the …
Comment: problem reading bam files into nucleR by efoss ▴ 10
I thought I might be able to track down a problematic line in the bam file by repeatedly splitting the bam file in and then running the co…
Answer: two questions about limma by Gordon Smyth 53k
Answered here: https://support.bioconductor.org/p/16318/
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication? by Ahmed Salah • 0
Thank you very much! will take that into consideration
Votes
Upcoming Ensembl API and data access changes - new blog post available
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Large logFC but somewhat high FDR
Comment: upstream git branches of bioc-release
Comment: upstream git branches of bioc-release
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