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Showing :
Normalization
•
reset
0
votes
4
replies
378
views
Limma- Print-tip loess normalisation-printer layout information does not match M row dimension
Normalization
limma
within-arraynormalisation
Printerlayout
7 days ago
dseauer
• 0
1
vote
2
replies
868
views
Consistent Decimals In Data After Antibody Array Background Correction Using Limma:nec Function
limma
MicroarrayData
Normalization
16 days ago • updated 15 days ago
Ai
• 0
0
votes
0
replies
107
views
Diffbind: Cut&Run: E-coli spike-in
Normalization
DiffBind
16 days ago
kim
• 0
0
votes
1
reply
332
views
Spike-in normalization method in ATACseq
sperm
Drosophila_melanogaster
ATACSeq
Normalization
SpikeIn
updated 17 days ago by
ATpoint
★ 4.5k • written 19 days ago by
Katerina
• 0
3
votes
4
replies
453
views
Are transformed values from rlog /vst log2 normalized counts?
Normalization
DESeq2
rlog
vst
5 weeks ago
Eva
▴ 10
0
votes
0
replies
134
views
Background normalization of Diffbind dba.normalize
Normalization
DiffBind
6 weeks ago
Young Boqin
• 0
1
vote
1
reply
225
views
Normalization RNA-seq for multiple plates
Normalization
DESeq2
RNASeqData
edgeR
updated 7 weeks ago by
ATpoint
★ 4.5k • written 7 weeks ago by
Pimpa
• 0
1
vote
5
replies
779
views
RNASeq normlization for samples with global shift in gene expression
qsmooth
DESeq2
limma
Normalization
updated 12 weeks ago by
Gordon Smyth
51k • written 3 months ago by
Xuan Yi
• 0
0
votes
2
replies
331
views
Analysis of transcription inhibition with DESeq2
SpikeIn
DESeq2
Normalization
written 3 months ago by
ADopico
• 0
1
vote
3
replies
846
views
Combining two bulk RNA seq datasets
RNASeqData
DESeq2
Normalization
5 months ago
Tanvi
• 0
2
votes
2
replies
501
views
Control group between 2 batches have different expression in RNAseq data
BatchQC
BatchEffect
rnaseqGene
Normalization
updated 5 months ago by
jessica.mcclintock
▴ 10 • written 6 months ago by
Đình Vinh
• 0
0
votes
2
replies
514
views
Normalisation using clinical traits in RNA seq using DESeq2
DESeq2
clinicaltraits
Normalization
batchnormalisation
6 months ago • updated 5 months ago
sukeshinik5
▴ 20
0
votes
1
reply
376
views
normalization across different species for DESeq2 analysis
DESeq2
normalization
Normalization
acrossspecies
updated 6 months ago by
Michael Love
42k • written 6 months ago by
piemmea
• 0
0
votes
1
reply
362
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 6 months ago by
ATpoint
★ 4.5k • written 6 months ago by
sajadahmad41454
• 0
2
votes
2
replies
896
views
[Diffbind] Blacklist error: Error: Invalid Normalization and Unable to apply Blacklist/Greylist
DiffBind
Normalization
7 months ago
Henry
▴ 10
1
vote
6
replies
828
views
DESeq2 step-by-step Normalization then DGE separately & Normalization methods and switchability
DESeq2
Normalization
updated 7 months ago by
Michael Love
42k • written 7 months ago by
RL
• 0
0
votes
2
replies
508
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
7 months ago
Marianna
▴ 20
1
vote
4
replies
640
views
EDAseq to normalise for both gene-length and GC-content
norma
EDASeq
EDA
Normalization
8 months ago
samuel.channon
• 0
0
votes
0
replies
375
views
TCGAbiolinks STAD project 450k Methylation Beta Value data
MethylationArray
normalize450K
TCGAbiolinks
Normalization
MethylationArrayData
8 months ago
Giovanni Calice
▴ 160
2
votes
1
reply
504
views
Normalization factors when there is a possible unidirectional expression
limma
Normalization
DifferentialExpression
updated 9 months ago by
ATpoint
★ 4.5k • written 9 months ago by
Shaimaa Gamal
• 0
2
votes
2
replies
983
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
9 months ago
jackafiore
• 0
2
votes
3
replies
660
views
TMM normalization after RUVg correction
RUVSeq
limma
DifferentialExpression
edgeR
Normalization
updated 9 months ago by
James W. MacDonald
67k • written 9 months ago by
Shaimaa Gamal
• 0
1
vote
3
replies
960
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics
Normalization
RNASeq
DESeq2
written 10 months ago by
georgii.vdovin
• 0
0
votes
1
reply
703
views
WGCNA Soft Power
Normalization
DESeq2
Network
RNASeqData
updated 10 months ago by
ATpoint
★ 4.5k • written 10 months ago by
Ömür Koray
• 0
2
votes
4
replies
1.4k
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
10 months ago
Sabiha
▴ 20
1
vote
1
reply
713
views
DESeq2 Normalization for QC Samples
DESeq2
Normalization
updated 10 months ago by
Michael Love
42k • written 10 months ago by
phmai1148
• 0
2
votes
2
replies
1.8k
views
DESeq2 median of ratios normalization instead of VST or rlog for ordinations?
DESeq2
medianofratiostransformation
StatisticalMethod
Normalization
dispersion
10 months ago
Linton
• 0
0
votes
0
replies
473
views
SomaticSignatures normalization for trinucleotide motifs in reference genome
Normalization
SomaticSignatures
12 months ago
vasily.dobrovolsky
• 0
0
votes
6
replies
1.5k
views
DESeq2 Normalization with 4 Groups
Normalization
RNASeq
DESeq2
13 months ago
erika.maldonado-rosado
• 0
0
votes
0
replies
492
views
Dual species RNA-seq, Library size and normalization
Normalization
edgeR
RNASeq
DESeq2
13 months ago
Florian
• 0
1
vote
11
replies
1.8k
views
DESeq2 gene length normalisation
Normalization
DESeq2
updated 13 months ago by
Michael Love
42k • written 13 months ago by
cm15245
• 0
1
vote
4
replies
4.0k
views
How to process Nimblegen microarray .pair files to get normalized expression matrix
Microarray
Preprocessing
NimbleGen
Normalization
written 24 months ago by
Sarah
• 0
1
vote
5
replies
1.4k
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI
normalization
limma
voom
Normalization
updated 16 months ago by
Gordon Smyth
51k • written 16 months ago by
manuelsokolov
• 0
0
votes
3
replies
838
views
Normalization of different input materials
Normalization
DESeq2
updated 17 months ago by
ATpoint
★ 4.5k • written 17 months ago by
altuda
▴ 10
0
votes
2
replies
1.1k
views
Can VST-transformed community composition data be expressed as relative abundance?
vst
relativeabundance
Normalization
DESeq2
17 months ago
dwas
• 0
5
votes
11
replies
4.9k
views
Visualization of negative log CPM values
Visualization
Normalization
ggplot2
edgeR
written 18 months ago by
Sabiha
▴ 20
0
votes
3
replies
976
views
Is the gene-specific PCR efficiency a serious concern for intrasample comparisons in RNA-Seq?
RNA-Seq
normalization
PCR_efficiency
bias
intrasample_comparison
updated 18 months ago by
James W. MacDonald
67k • written 18 months ago by
FedeXandeR
• 0
1
vote
2
replies
1.2k
views
Filter by expression and keep probes in limma
R
filter
limma
Normalization
Microarray
19 months ago
Abdul
▴ 20
2
votes
2
replies
883
views
Question about transforming log expression values to antilog values in limma
IlluminaChip
limma
AffymetrixChip
Normalization
Microarray
19 months ago
Abdul
▴ 20
1
vote
2
replies
792
views
RNA-Seq normalization of pre and post treatment groups
Normalization
RNASeq
written 19 months ago by
Sarah
• 0
0
votes
2
replies
1.1k
views
Scaling and normalization by deconvolution of SMART-Seq 2 data
scran
Clustering
SingleCell
Normalization
20 months ago
Assa Yeroslaviz
★ 1.5k
1
vote
6
replies
2.3k
views
can negative value microarray matrix data analysed by limma directly
limma
microarray
normalization
updated 15 months ago by
Gordon Smyth
51k • written 4.2 years ago by
linouhao
▴ 20
2
votes
3
replies
2.6k
views
deseq2 normalization and statistical method
normalization
deseq2
StatisticalMethod
updated 20 months ago by
ATpoint
★ 4.5k • written 20 months ago by
SH
▴ 10
2
votes
1
reply
1.4k
views
deseq2 RLE normalization
RLE
normalization
deseq2
updated 20 months ago by
ATpoint
★ 4.5k • written 20 months ago by
SH
▴ 10
0
votes
1
reply
843
views
Using DESeq2 only for normalization
DESeq2
Normalization
updated 20 months ago by
ATpoint
★ 4.5k • written 20 months ago by
phmai1148
• 0
0
votes
1
reply
579
views
processing samples with significantly different counts
DESeq2
Normalization
updated 20 months ago by
ATpoint
★ 4.5k • written 20 months ago by
Anastasiia
• 0
0
votes
5
replies
1.7k
views
DESeq Normalization and miRNAs Filtering
DESeq2
Normalization
updated 20 months ago by
ATpoint
★ 4.5k • written 20 months ago by
phmai1148
• 0
2
votes
3
replies
1.3k
views
Batch correction and Deseq2
DESeq2
WGCNA
Normalization
NormalyzerDE
BatchEffect
updated 20 months ago by
ATpoint
★ 4.5k • written 20 months ago by
suphamon.j
▴ 20
0
votes
1
reply
773
views
Normalization of RNA seq data before DESeq2 and PCA in case of strong batch effects
DESeq2
Normalization
sva
updated 20 months ago by
ATpoint
★ 4.5k • written 20 months ago by
marslena
• 0
3
votes
3
replies
1.0k
views
What is cpmByGroup used for?
edgeR
Normalization
updated 21 months ago by
James W. MacDonald
67k • written 21 months ago by
EM
• 0
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Comment: Is it recommended to analyze different tissues together when using edgeR
by
Gordon Smyth
51k
Just plot the samples with plotMDS(). Alternatively you would use `voomLmFit()` with `sample.weights=TRUE` or `var.group=tissue`. In princ…
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• 0
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67k
The advantage to using the LRT is that it's an omnibus test that tells you if there are changes at any time point. If you have lots of time…
Votes
Answer: Using voom-transformed counts for unsupervised analyses (PCA, Random Forest, Ela
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