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SCRAN
•
reset
1
vote
3
replies
138
views
Incosistencies in buildSNNGraph() function
scran
seurat
written 5 days ago by
Lucas
• 0
0
votes
2
replies
1.1k
views
Memory issue while using "quickcluster" and "computeSumFactors" functions on single cell RNA seq dataset with 7-9 lac cells
scran
updated 7 months ago by
ATpoint
★ 5.0k • written 8 months ago by
amlanjyoti
• 0
1
vote
2
replies
1.5k
views
Help with the code for "BiocNeighbors::generic_find_knn() function used by scran::quickCluster()
BiocNeighbors
generic_find_knn
scran
quickCluster
9 months ago
Arctic
• 0
2
votes
2
replies
1.2k
views
SingleR with multiple single cell references
SingleR
scran
11 months ago • updated 9 months ago
maltethodberg
▴ 180
0
votes
0
replies
1000
views
modelGeneVar in MulticoreParam: wrong args for environment subassignment
BiocParallel
scran
BiocParall
16 months ago
Lluís Revilla Sancho
▴ 760
0
votes
5
replies
1.1k
views
modelGeneVarByPoisson error selecting bandwidth automatically
scran
updated 18 months ago by
Peter Hickey
▴ 760 • written 18 months ago by
Lluís Revilla Sancho
▴ 760
0
votes
0
replies
502
views
Differential Gene Wrapper for Propotion of Detected
scran
19 months ago
Dario Strbenac
★ 1.6k
0
votes
4
replies
1.3k
views
Single Cell Experiment : Differential Expression "List of length 0" - Help!
DifferentialExpression
scran
scater
SingleCellExperiment
edgeR
19 months ago
vohara4
• 0
1
vote
2
replies
835
views
Reproducibility issue with scran together with lapply and BiocParallel
scran
BiocParallel
bluster
21 months ago
ATpoint
★ 5.0k
2
votes
1
reply
1.1k
views
Existence of Bioconductor versus Seurat Software Ecosystems Quantitative Comparison
scran
bluster
scater
scuttle
updated 22 months ago by
ATpoint
★ 5.0k • written 22 months ago by
Dario Strbenac
★ 1.6k
0
votes
3
replies
1.6k
views
scran package: quickCluster and calculateSumFactors parallelization
BiocParallel
scran
updated 2.0 years ago by
ATpoint
★ 5.0k • written 2.0 years ago by
qwang178
• 0
1
vote
2
replies
2.1k
views
ScRNAseq analysis scran :: quickcluster Error
scRNAseq
quickcluster
scRNA
scran
2.1 years ago
ruba-mahmoud
• 0
0
votes
1
reply
1.2k
views
Speeding up large scale scRNAseq analyses in R and improve memory
scran
updated 2.4 years ago by
ATpoint
★ 5.0k • written 2.4 years ago by
Maximilian
• 0
0
votes
2
replies
2.0k
views
runPCA in a SingleCellExperiment results ina colMeans error
scran
SingleCellExperiment
2.6 years ago
Lluís Revilla Sancho
▴ 760
2
votes
2
replies
11k
views
scran ScoreMarkers throws a warning
scoreMarkers
scran
updated 2.6 years ago by
Peter Hickey
▴ 760 • written 2.6 years ago by
Assa Yeroslaviz
★ 1.5k
2
votes
3
replies
2.2k
views
P-value histogram of testLinearModel is not uniform
smart-seq2
scRNAseq
scran
2.6 years ago
Lluís Revilla Sancho
▴ 760
0
votes
2
replies
1.7k
views
Scaling and normalization by deconvolution of SMART-Seq 2 data
scran
Clustering
SingleCell
Normalization
2.8 years ago
Assa Yeroslaviz
★ 1.5k
1
vote
0
replies
1.4k
views
Use scran's normalization by deconvolution for each individual sample or over all samples
scran
Normalization
scRNA
BatchEffect
3.0 years ago
r_grau
▴ 10
4
votes
3
replies
2.8k
views
SingleCellExperiment: Edit cluster IDs in colLabels
scran
Clustering
SingleCellExperiment
annotate
cluster
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
achoppe
▴ 10
1
vote
1
reply
1.4k
views
setting sizeFactors from scran :: caculateSumFactors.
scran
DESeq2
updated 3.5 years ago by
ATpoint
★ 5.0k • written 3.5 years ago by
OE
• 0
3
votes
3
replies
2.0k
views
RFC: Inconsistent software license for scran & automated license checks
agpl
license
gpl
scran
3.6 years ago • updated 3.4 years ago
matthias-doering
▴ 40
2
votes
1
reply
2.0k
views
Getting error Creating SingleCellExperiment instances "all assays must have the same nrow and ncol"
SingleCellExperiment
scran
scater
updated 3.8 years ago by
Basti
▴ 780 • written 3.8 years ago by
Lucky
• 0
2
votes
2
replies
2.3k
views
scuttle:: librarySizeFactors returning non-finite values
normalizeCounts
scran
librarySizeFactors
scuttle
scRNAseq
3.9 years ago
behr201
• 0
1
vote
2
replies
1.6k
views
How to answer reviewers question of physical cell size influencing single cell results?
SingleCellExperiment
scater
ExperimentalDesign
scran
3.9 years ago
msn
▴ 10
2
votes
2
replies
1.6k
views
Block weights in scran::pairwiseTTests
scran
4.2 years ago
Angelos Armen
• 0
1
vote
1
reply
1.8k
views
scran computeSumFactors showing error
scran
single
cell
updated 4.2 years ago by
Aaron Lun
★ 29k • written 4.2 years ago by
hemantcnaik
• 0
3
votes
3
replies
2.7k
views
Ensuring reproducibility in BiocParallel SingleCellExperiment workflows
scater
SingleCellExperiment
BiocParallel
scuttle
scran
4.2 years ago
enricoferrero
▴ 680
2
votes
2
replies
2.9k
views
loading scran package
scran
installation
4.9 years ago
TJ
• 0
5
votes
6
replies
2.8k
views
DE Analysis on Scran-Normalized Counts
DifferentialExpression
scRNAseq
scran
updated 4.9 years ago by
Aaron Lun
★ 29k • written 4.9 years ago by
LUCIA
• 0
4
votes
5
replies
4.0k
views
scuttle::aggregateAcrossCells() crashes R on large SingleCellExperiment
scran
SingleCellExperiment
scuttle
scater
updated 5.0 years ago by
Aaron Lun
★ 29k • written 5.0 years ago by
enricoferrero
▴ 680
0
votes
0
replies
1.4k
views
Creating a stable batch-corrected scRNA-Seq data with multiple datasets and multiple integrations
scater
singlecell
scran
BatchEffect
5.2 years ago
hamza_karakurt
▴ 60
1
vote
1
reply
1.5k
views
scran::computeSumFactors with single-cell ATAC-seq data
scran
updated 5.2 years ago by
Aaron Lun
★ 29k • written 5.2 years ago by
Angelos Armen
• 0
1
vote
1
reply
2.8k
views
findMarkers() {scran} doesn't really find markers
findMarkers
scran
updated 5.4 years ago by
Aaron Lun
★ 29k • written 5.4 years ago by
paula.vaccarello
• 0
2
votes
3
replies
2.9k
views
multiBatchNorm vs. calculateSumFactors on concatenated dataset
batchelor
scran
updated 5.4 years ago by
Aaron Lun
★ 29k • written 5.4 years ago by
Angelos Armen
• 0
5
votes
4
replies
9.1k
views
scRNA-seq pseudo-bulk differential expression analysis with pseudobulkDGE()
scran
scater
scuttle
updated 5.4 years ago by
Aaron Lun
★ 29k • written 5.4 years ago by
enricoferrero
▴ 680
2
votes
6
replies
2.0k
views
Why is an averaged reference used in DESeq2 normalisation?
deseq2
scran
batchelor
updated 5.6 years ago by
Aaron Lun
★ 29k • written 5.6 years ago by
Angelos Armen
• 0
9
votes
4
replies
7.2k
views
Removing cell cycle genes in large data set integration
scran
cyclone
updated 5.6 years ago by
Aaron Lun
★ 29k • written 5.6 years ago by
p.joshi
▴ 40
0
votes
4
replies
2.1k
views
scran's quickCluster error
scran
updated 5.7 years ago by
Aaron Lun
★ 29k • written 5.7 years ago by
marc.tormo
• 0
3
votes
1
reply
1.6k
views
Output lfc for Wilcox-based findMarkers
scran
updated 5.7 years ago by
Aaron Lun
★ 29k • written 5.7 years ago by
ATpoint
★ 5.0k
7
votes
5
replies
4.9k
views
Optimal cluster number identification using buildSNNgraph and igraph clusters
scran
gap statistics
single cell clustering
updated 5.7 years ago by
Aaron Lun
★ 29k • written 5.7 years ago by
p.joshi
▴ 40
7
votes
3
replies
8.4k
views
Downsample or not to downsample?
scrna-seq
downsampleBatches
scran
updated 5.7 years ago by
Aaron Lun
★ 29k • written 5.7 years ago by
p.joshi
▴ 40
0
votes
3
replies
1.7k
views
How can doubletCell() scores be converted to single/doublet annotation?
scran
doublet
updated 5.8 years ago by
Aaron Lun
★ 29k • written 5.8 years ago by
thkapell
▴ 10
2
votes
1
reply
3.0k
views
Batch Corrections (MNN) with TPM values
scRNA-Seq
scater
scran
rna-seq
MNN
updated 6.3 years ago by
Aaron Lun
★ 29k • written 6.3 years ago by
hamza_karakurt
▴ 60
1
vote
1
reply
1.3k
views
Stouffer's method weights in scran::pairwiseWilcox
scran
updated 6.3 years ago by
Aaron Lun
★ 29k • written 6.3 years ago by
Angelos Armen
• 0
0
votes
5
replies
3.9k
views
Cannot install Bioconductor v3.9
scran
cellassign
bioconductor
updated 6.3 years ago by
Martin Morgan
25k • written 6.3 years ago by
thkapell
▴ 10
1
vote
2
replies
2.0k
views
Mitigating cell cycle effect in scRNA-seq using a blocking factor or design matrix
scran
updated 6.3 years ago by
Aaron Lun
★ 29k • written 6.3 years ago by
s1437643
▴ 20
2
votes
1
reply
2.2k
views
scran: excluding genes with extremely high expression during normalization?
single-cell RNA-seq
scran
normalization
updated 6.4 years ago by
Aaron Lun
★ 29k • written 6.4 years ago by
daniel.borshagovski
• 0
1
vote
3
replies
1.5k
views
k argument in buildSNNGraph
scran
updated 6.4 years ago by
Aaron Lun
★ 29k • written 6.4 years ago by
Angelos Armen
• 0
1
vote
5
replies
2.5k
views
Preprocessing in scran::doubletCells
scran
simpleSingleCell
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
Angelos Armen
• 0
4
votes
4
replies
3.8k
views
using limma and edgeR for differential expression of Drop-seq/10xGenomics scRNA-seq data
limma
edgeR
simplesingleCell
scran
scater
updated 6.5 years ago by
Aaron Lun
★ 29k • written 6.5 years ago by
Bogdan
▴ 670
100 results • Page
1 of 2
Recent ...
Replies
Answer: When to use edgeR or limma
by
Gordon Smyth
53k
The short answer is that we generally prefer edgeR for applications with lots of small counts and limma for complex designs with random eff…
Comment: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Gordon Smyth
53k
I updated the above answer today.
Answer: When to use edgeR or limma
by
James W. MacDonald
68k
I would use edgeR's quasi-likelihood model for any analysis with a smaller number of observations (like 3 vs 3 or similar), but if you have…
Answer: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Gordon Smyth
53k
The short answer is that limpa reads standard feature-level intensities from Spectronaut or other quantifications tools. limpa is designed …
Comment: Question about the filterByExpr function inputs in edgeR
by
mohammedtoufiq91
▴ 10
@gordonsmyth Noted, thank you very much.
Votes
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
A: deseq2 - paired samples in 2 sequencing types with very different library sizes
Comment: Question about the filterByExpr function inputs in edgeR
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