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23
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The difference when using Limma compare between 3 conditions vs 2 conditions
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5 weeks ago • updated 3 days ago Chris • 0
14
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Why p value inconsistent when using different number of pathway score?
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13 days ago • updated 2 days ago Chris • 0
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SGSeq: moving toward diffex from SGSeq analysis
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updated 3 days ago by ▴ 200 • written 6.7 years ago by ▴ 80
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19
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DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
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updated 3 days ago by • 0 • written 4.6 years ago by ▴ 10
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6
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Although I successfully installed the package "simpleaffy'' , I cannot library it. Why?
simpleaffy
updated 1 day ago by 65k • written 2.3 years ago by • 0
3
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3
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206
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DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
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updated 5 days ago by ★ 4.1k • written 5 days ago by ▴ 10
3
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4
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189
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Help with error using camera() in limma
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3 days ago Chris • 0
3
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2 days ago shepherl 3.9k
2
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2
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Perform gene set enrichment analysis using fgsea on two conditions that don't have differential express genes
fgsea
4 days ago Chris • 0
2
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2
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limma for gene expression analysis with big and unbalanced sample size
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updated 3 days ago by • 0 • written 7.9 years ago by • 0
2
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2
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98
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Too many significant genes when integrating gtex and tcga
RNA-seq DESeq2
updated 18 hours ago by 41k • written 18 hours ago by • 0
1
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2
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276
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CombineArrays for EPIC and EPIC V2
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4 weeks ago • updated 16 hours ago Kim • 0
1
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4
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473
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DEseq2 coefficient
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10 weeks ago • updated 2 days ago JKim • 0
1
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2
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248
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limma Intercept vs No-intercept models completely changing DMR results?
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updated 12 hours ago by 50k • written 16 hours ago by • 0
1
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1
reply
93
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Fold change calculation in Diffbind vs. DESEQ2?
DiffBind ChIPSeqData DESeq2
updated 4 days ago by 41k • written 4 days ago by • 0
0
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2
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153
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limma-voom: Calculate the log-fold change in gene expression within a sample
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written 11 days ago by • 0
0
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3
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110
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Differences in differential analysis methods for RNA-Seq
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updated 2 days ago by ★ 4.1k • written 2 days ago by • 0
0
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1
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40
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Job: Postdoctoral Position in Bioinformatics - Mainz, Germany
JobPosting
written 2 days ago by • 0
0
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2
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105
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More regions in union when increasing DBA$config$mergeOverlap
DiffBind
3 days ago • updated 1 day ago Ian D. ▴ 70
0
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0
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28
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How to create consensus peak sets for each group to use in DiffBind?
DiffBind
1 day ago Ian D. ▴ 70
0
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0
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36
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DiffBind: no peaks in DBA object
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1 day ago yvonneh • 0
0
votes
2
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74
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DESeq2 v1.35.0
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updated 1 day ago by 41k • written 1 day ago by • 0
0
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0
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29
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flowStats for CyTOF
flowStats
1 day ago tholmes • 0
0
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1
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76
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Gene-by-sample covariates in limma
Metabolomics limma
updated 1 day ago by 50k • written 1 day ago by • 0
0
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1
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81
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NEED FOR NESSASORY HELP
RNASeqData edgeR
updated 1 day ago by 50k • written 1 day ago by • 0
0
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2
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107
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Deseq2 and Normalization of RNA sequencing data
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2 days ago • updated 1 day ago kcarey • 0
0
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1
reply
168
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Why does GSEA on edgeR results for randomized samples give highly significant p-values?
fgsea edgeR
updated 1 day ago by 50k • written 1 day ago by ▴ 10
0
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0
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105
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One error problem occurred caused by data type during using GDCprepare function of TCGAbiolinks to handle TCGAbiolinks BRCA data
GDCRNATools TCGA
9 days ago • updated 1 day ago glaciya2018 • 0
0
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2
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108
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Timecourse RNASeq analysis
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4 days ago • updated 1 day ago Aurora • 0
0
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0
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29
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dba.plotProfile() plotting and interpretation
DiffBind
20 hours ago Henry ▴ 10
0
votes
0
replies
31
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Handling multiple differential expression comparisons
DESeq2
19 hours ago Zainab • 0
0
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0
replies
69
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Empty table plot using plotGseaTable()
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2 days ago • updated 16 hours ago Chris • 0
0
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1
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43
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package goseq seems to be not available on the latest version of R
goseq
updated 14 hours ago by 50k • written 17 hours ago by • 0
0
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0
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78
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how to do Differential splicing from SGSeq analysis
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9 days ago • updated 5 days ago Sara • 0
0
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6
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151
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Once again a "Model matrix not full rank"
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updated 7 hours ago by ★ 1.3k • written 1 day ago by ▴ 130
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4
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240
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Minfi - estimatecellscount
min minfi
updated 4 days ago by 65k • written 5 weeks ago by • 0
0
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0
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63
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Could we detect difference at pathway level but not significant different at gene level?
GSVA
4 days ago Chris • 0
0
votes
1
reply
93
views
putting my own expression data in deseq2
DESeq2
updated 4 days ago by ★ 4.1k • written 4 days ago by • 0
0
votes
1
reply
70
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Version 3.15 S4Vectors packages are missing
S4Vectors
updated 4 days ago by 3.9k • written 4 days ago by • 0
0
votes
0
replies
51
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News: BioC2024 deadlines approaching
BioC2024 Bioconductor Community
4 days ago Maria Doyle ▴ 70
0
votes
1
reply
92
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Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 4 days ago by 41k • written 4 days ago by • 0
0
votes
3
replies
157
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Different number of exons in reference genome affects DEXSeq analysis
DEXSeq
updated 3 days ago by ★ 1.9k • written 16 days ago by • 0
0
votes
3
replies
223
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery ArrayExpress limma DifferentialExpression
updated 3 days ago by 65k • written 4 days ago by • 0
0
votes
1
reply
214
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots HMMcopy CopyNumberVariation
updated 3 days ago by 65k • written 4 days ago by • 0
0
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3
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1.5k
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Limma, eBayes different sample size of groups and adjustment
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updated 3 days ago by • 0 • written 13.0 years ago by ▴ 10
0
votes
3
replies
132
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Store output of matchPWM()
biostrings Biostrings
updated 2 days ago by 65k • written 2 days ago by ▴ 70
0
votes
1
reply
118
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
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updated 2 days ago by ★ 4.1k • written 2 days ago by • 0
0
votes
3
replies
231
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 2 days ago by 41k • written 3 days ago by • 0
0
votes
2
replies
206
views
Get genomic coordinates of CpGs sites (chromosomes, genomic position)
GenomeWideAssociation SequenceAnnotation MethylationArray GenomicDistributionsData
3 days ago • updated 2 days ago Yveto • 0
49 results • Page 1 of 1
Recent ...
Replies
Answer: Once again a "Model matrix not full rank" by swbarnes2 ★ 1.3k
Replicate numbers, like the 1 in control_1 are fine in sample names, but never add them to anything else in colData. From the computer's p…
Answer: limma Intercept vs No-intercept models completely changing DMR results? by Gordon Smyth 50k
I'll add a little bit of general advice to James' answer. You seem to have the misunderstanding that you can change the design matrix but …
Answer: package goseq seems to be not available on the latest version of R by Gordon Smyth 50k
It is true that goseq isn't available for Bioc 3.19. That is because goseq depends on txbmaker, which is itself not yet available for Bioc…
Answer: limma Intercept vs No-intercept models completely changing DMR results? by James W. MacDonald 65k
This part: ``` design_intercept <- model.matrix(~Alt+Sex+Age+PC1, data=targets_Sherpa) colnames(design_intercept) <- c("AltSHP_LA","Al…
Comment: CombineArrays for EPIC and EPIC V2 by Kim • 0
Thank you Tim, this is a great help in getting me started!
Votes
Answer: limma Intercept vs No-intercept models completely changing DMR results?
Answer: CombineArrays for EPIC and EPIC V2
Answer: Too many significant genes when integrating gtex and tcga
Comment: Too many significant genes when integrating gtex and tcga
A: Print Differentially Expressed Exons From Dexseq Results
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