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Comment: Using DESeq2 on single cell RNA Seq data of same cell type but different conditi
by
ATpoint
★
4.9k
No, cells from the same donor are correlated due to the donor effect. There is literature on this you should read to get started.
https:/…
Comment: Using DESeq2 on single cell RNA Seq data of same cell type but different conditi
by
Raya
•
0
Wouldn't each sample in the single cell rna seq act as a biological replicate? each condition (eg: mab3_20C) has ~70 single cell samples.
Comment: Getting way too many DEGs with LRT for time-series RNA-seq!
by
James W. MacDonald
68k
>Sequential post-exposure measurements of the same set of genes is not the same thing as having multiple treated groups!
They are not the…
Comment: Getting way too many DEGs with LRT for time-series RNA-seq!
by
Denise
•
0
Sequential post-exposure measurements of the same set of genes is not the same thing as having multiple treated groups! And I suppose ther…
Comment: Using DESeq2 on single cell RNA Seq data of same cell type but different conditi
by
ATpoint
★
4.9k
There is no biological replication here. This is not supported by DESeq2 (or mos credible statistical tools). You would need different dono…
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