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rnaseqdata
•
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0
votes
1
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76
views
How to find degraded genes but unaffected by condition in a time course RNAseq experiment ?
DESeq2
RNASeqData
DifferentialExpression
updated 17 days ago by
ATpoint
★ 2.1k • written 17 days ago by
Julie
• 0
0
votes
1
reply
76
views
Differential RNA seq on TCGA set
DESeq2
RNASeqData
updated 24 days ago by
ATpoint
★ 2.1k • written 24 days ago by
SilverCat
• 0
1
vote
4
replies
135
views
Inverted Log2FC values after DEseq2 analysis
DESeq2
Log2FC
RNASeqData
4 weeks ago
Iwan
• 0
0
votes
1
reply
141
views
Using DESeq2 for time-series analysis with two conditions
DESeq2
RNASeqData
updated 7 weeks ago by
ATpoint
★ 2.1k • written 7 weeks ago by
perceval.vellosillo
• 0
0
votes
1
reply
127
views
Single-Cell Data Simulation from Bulk RNA-seq
SingleCell
RNASeq
RNASeqData
scRNAseq
updated 11 weeks ago by
Gordon Smyth
47k • written 11 weeks ago by
irvin.ng
• 0
0
votes
0
replies
132
views
how to create PHENO_DATA
RNASeq
RNASeqData
RNASeqRData
4 months ago
marymshahi2021
• 0
1
vote
2
replies
433
views
Understanding how to contrast two treatments in DESeq2
DESeq2
RNASeqData
updated 5 months ago by
swbarnes2
★ 1.2k • written 5 months ago by
Jennifer M
• 0
0
votes
0
replies
148
views
Can I loosely say that a gene is expressed by simply look at the gene count from RNA-Seq?
RNASeqData
RNASeq
6 months ago
Pang
• 0
0
votes
1
reply
223
views
I have a query regarding differential gene expression using limma-voom.
RNASeqData
DESeq2
updated 6 months ago by
Gordon Smyth
47k • written 6 months ago by
btadfaramin
• 0
1
vote
7
replies
485
views
DESeqDataSetFromMatrix error
BiocGenerics
DESeq2
DEGreport
RNASeqData
DEG
updated 6 months ago by
James W. MacDonald
62k • written 6 months ago by
Diya
• 0
2
votes
9
replies
802
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA
r
RNASeqData
voom
limma
7 months ago
Peipei
• 0
1
vote
5
replies
422
views
Error in DEseq
DEseq
RNASeqData
updated 8 months ago by
ATpoint
★ 2.1k • written 8 months ago by
Sanatan
• 0
1
vote
1
reply
301
views
NOIseq Probability column
RNAseq
RNASeqData
NOISeq
8 months ago • updated 7 months ago
Pratibha
▴ 10
3
votes
3
replies
437
views
How to formulate 2 factor nested design in DESeq2
nested_design
RNASeqData
DESeq2
8 months ago
JK Kim
▴ 10
0
votes
2
replies
560
views
How to run vst transformation
RNASeqData
vst
updated 8 months ago by
Michael Love
39k • written 9 months ago by
sropri
• 0
2
votes
3
replies
486
views
Problem with tximport IDs
tximport
tx2gene
rnas
DESeq2
RNASeqData
9 months ago
Maka
• 0
0
votes
5
replies
891
views
Can Differential Isoform expression analysis can be performed using DESeq2 package
RNASeqData
isoform
DESeq2
9 months ago • updated 8 months ago
priyanka.m
• 0
22
votes
14
replies
3.8k
views
biomaRt error unable to fix: need help troubleshooting
biomaRt
RNASeqData
R
updated 9 months ago by
Neri0304
• 0 • written 9 months ago by
Dina
▴ 50
0
votes
2
replies
547
views
Condition shown as 'Untreated vs. Treated' in output of results() in DESeq2
RNASeqData
DESeq2
updated 10 months ago by
Michael Love
39k • written 10 months ago by
treebig44
▴ 30
3
votes
2
replies
377
views
DEX-Seq returns Error in FUN(X[[i]], ...) : subscript out of bounds when I use DEXSeqDatasetFromHTseq()
R
DEXSeq
SequenceAnnotation
RNASeqData
10 months ago • updated 9 months ago
Dakarai
▴ 10
0
votes
1
reply
329
views
Differential Expression Analysis-Input is DESeq Standardized
rnaseq
RNASeqData
limma
limmaGUI
updated 10 months ago by
Michael Love
39k • written 10 months ago by
sushimoto
• 0
0
votes
2
replies
415
views
lncRNA Genes in a Dataset
ncRNAtools
RNASeqData
10 months ago
sushimoto
• 0
0
votes
0
replies
235
views
edgeR testing interactions between two factors while controlling for blocking factor
edgeR
ExpressionData
RNASeqData
10 months ago
Mafalda
• 0
1
vote
4
replies
548
views
lncRNA Differential Expression Analysis
DESeq2
limma
RNASeqData
10 months ago
sushimoto
• 0
0
votes
0
replies
278
views
How to perform transcription factor enrichment analysis (TFEA) for RNA-seq counts data.
TFEA.ChIP
RNASeq
RNASeqData
10 months ago
treebig44
▴ 30
0
votes
0
replies
335
views
high number of reads mapped to too many loci
RNASeqData
10 months ago
vasa.broz
• 0
0
votes
1
reply
244
views
HSMMSingleCell metadata
ExperimentHubData
ExpressionData
CellDataSet
RNASeqData
10 months ago
karl.stamm
▴ 10
4
votes
1
reply
758
views
Finding significant up-regulated and down-regulated genes given a log2FoldChange threshold
DESeq2
RNASeq
RNASeqData
updated 10 months ago by
Michael Love
39k • written 10 months ago by
treebig44
▴ 30
1
vote
1
reply
280
views
Strange error while running DESeq() function.
DESeq2
RNASeq
RNASeqData
updated 10 months ago by
Michael Love
39k • written 10 months ago by
treebig44
▴ 30
2
votes
6
replies
4.0k
views
How to correct for age, sex, etc. from an RNA-seq data in DESeq2.
DESeq2
RNASeq
RNASeqData
10 months ago
treebig44
▴ 30
0
votes
1
reply
575
views
Limma interaction term
RNASeqData
limma
DifferentialExpression
updated 11 months ago by
Gordon Smyth
47k • written 11 months ago by
bageerathan.v
• 0
1
vote
2
replies
341
views
Pathway analysis of RNAseq data using goseq package
Pathways
goseq
RNASeqData
11 months ago • updated 10 months ago
m.glymenaki
• 0
1
vote
1
reply
349
views
edgeR for SDE analysis
RNASeqRData
edgeR
RNASeqR
RNASeqData
updated 11 months ago by
Gordon Smyth
47k • written 11 months ago by
vikycairoli
• 0
3
votes
3
replies
508
views
Replicating iDEP parameters in DESeq2; two conditions and an interaction term.
DESeq2
idep
RNASeqData
updated 11 months ago by
swbarnes2
★ 1.2k • written 11 months ago by
student2
• 0
0
votes
1
reply
544
views
'Negative' and 'Positive' bigWig RNAseq files: how to deal with these???
bigWig
RNASeqData
12 months ago
bas_work
▴ 10
35 results • Page
1 of 1
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Replies
Comment: Not able to download recount3 data
by
Leonardo Collado Torres
★ 1.0k
Please don't reopen that issue thread, as it will ping lots of people. Can you try using the newly documented Amazon AWS mirror? ```R lib…
Comment: mas5calls function doesn't work; mirna40cdf problem
by
Berna
• 0
Thanks for all answers. I have tried, but still get an error message. Do you know how I can work around this? > library(pd.mir…
Answer: struggling to create a heatmap
by
ATpoint
★ 2.1k
I am reluctant to read through that wall of code. Here is what would work. Note that prefiltering makes sense before, not after running `DE…
Answer: collapseReplicates error after tximeta
by
Michael Love
39k
The issue is that `collapseReplicates` just collapses `counts` and doesn't account for multiple assays which you need for e.g. Swish or oth…
Comment: Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it
by
ATpoint
★ 2.1k
That having said, I recommend to inspect the MA-plots (`plotMA()`), at best the ones produced right from `res <- results(dds); plotMA(res)`…
Votes
Significant reporter activity with mpralm function
Answer: I cannot install annotables package in Rstudio (macOS)
Answer: log2 of normalized counts for further analyzes
Answer: Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it
Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it should *not* be used
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• 0
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to
Guido Hooiveld
★ 3.5k
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James W. MacDonald
62k
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Michael Love
39k
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