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Views
Votes
1
vote
4
replies
360
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
26 days ago • updated 24 days ago
winwater0928
• 0
1
vote
0
replies
335
views
News:
Last Call: Nominations for Bioconductor Awards 2024! Deadline May 15
Recognition
Awards
Bioconductor
Bioc204
8 weeks ago • updated 5 days ago
shepherl
3.9k
4
votes
5
replies
334
views
Method to find pathways different between 2 groups
fgsea
GSVA
CAMERA
14 days ago • updated 4 days ago
Chris
▴ 10
3
votes
1
reply
329
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 26 days ago by
Michael Love
42k • written 26 days ago by
i.sudbery
▴ 40
0
votes
5
replies
325
views
Error for AnnotationForge makeOrgPackageFromNCBI function
OrgDb
AnnotationForge
updated 25 days ago by
James W. MacDonald
65k • written 28 days ago by
Gayatri
• 0
5
votes
3
replies
317
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 10 days ago by
Gordon Smyth
50k • written 4 weeks ago by
Shaimaa Gamal
• 0
2
votes
5
replies
313
views
get BM error
ensembldb
updated 25 days ago by
James W. MacDonald
65k • written 26 days ago by
Fara
• 0
1
vote
3
replies
305
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 12 days ago by
ATpoint
★ 4.1k • written 18 days ago by
angelathuynh5
• 0
3
votes
5
replies
302
views
Help with running egsea()
EGSEA
13 days ago • updated 12 days ago
Chris
▴ 10
0
votes
6
replies
302
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 16 days ago by
swbarnes2
★ 1.4k • written 17 days ago by
arfranco
▴ 130
0
votes
3
replies
302
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 18 days ago by
Michael Love
42k • written 19 days ago by
HAK
• 0
1
vote
2
replies
294
views
Get genomic coordinates of CpGs sites (chromosomes, genomic position)
GenomeWideAssociation
SequenceAnnotation
MethylationArray
GenomicDistributionsData
19 days ago • updated 18 days ago
Yveto
• 0
3
votes
4
replies
289
views
Store output of matchPWM()
biostrings
Biostrings
18 days ago • updated 6 days ago
mat149
▴ 70
2
votes
4
replies
287
views
Error EPICv2 ChAMP
ChAMPdata
IlluminaHumanMethylationEPICv2anno.20a1.hg38
ChAMP
updated 27 days ago by
James W. MacDonald
65k • written 4 weeks ago by
Mirella Aurora
• 0
2
votes
2
replies
285
views
error in limma , contrast.matrix Number of rows of contrast matrix must match number of coefficients in fit
limma
12 days ago • updated 7 days ago
zzrammal
• 0
0
votes
3
replies
282
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
updated 19 days ago by
James W. MacDonald
65k • written 20 days ago by
SSSJec
• 0
1
vote
2
replies
280
views
Multi-factorial, longitudinal disease progression analysis with unbalanced patient sampling - duplicateCorrelation or blocking?
rna-seq
duplicateCorrelation
limma
voom
11 days ago • updated 10 days ago
Dylan.Sheerin
• 0
2
votes
3
replies
277
views
package goseq seems to be not available on the latest version of R
goseq
updated 14 days ago by
Gordon Smyth
50k • written 16 days ago by
Patrycja
▴ 20
3
votes
3
replies
271
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 21 days ago by
ATpoint
★ 4.1k • written 21 days ago by
bioinf
▴ 10
0
votes
2
replies
268
views
featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_get_next_read_BIN: Assertion `l_name < 256' failed. Aborted (core dumpe…
metaMSdata
27 days ago • updated 25 days ago
shuwangxinze1996
• 0
0
votes
1
reply
266
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots
HMMcopy
CopyNumberVariation
updated 19 days ago by
James W. MacDonald
65k • written 20 days ago by
SSSJec
• 0
3
votes
4
replies
266
views
Help with error using camera() in limma
CAMERA
limma
19 days ago
Chris
▴ 10
0
votes
4
replies
265
views
Minfi - estimatecellscount
min
minfi
updated 20 days ago by
James W. MacDonald
65k • written 7 weeks ago by
clacarion
• 0
4
votes
9
replies
264
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 4 days ago by
ATpoint
★ 4.1k • written 5 days ago by
pangtaihin
• 0
2
votes
5
replies
259
views
How to retrieve gene ontology GO class from gene list?
GO.db
goTools
updated 24 days ago by
James W. MacDonald
65k • written 26 days ago by
bandconductor
• 0
2
votes
3
replies
257
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 15 days ago by
ATpoint
★ 4.1k • written 16 days ago by
Reza
• 0
1
vote
3
replies
255
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 24 days ago by
Michael Love
42k • written 24 days ago by
JalapenoCornbread
• 0
1
vote
3
replies
254
views
How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
DifferentialExpression
TimeCourse
timeOmics
edgeR
RNAseq
updated 5 days ago by
Gordon Smyth
50k • written 6 days ago by
Ashley
• 0
3
votes
3
replies
253
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 27 days ago by
Michael Love
42k • written 27 days ago by
WardDeb
• 0
1
vote
2
replies
249
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
DESeq2
eigencorplot
PCAtools
6 days ago • updated 3 days ago
BioinfGuru
▴ 30
0
votes
2
replies
245
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
4 weeks ago • updated 28 days ago
catv
• 0
2
votes
2
replies
238
views
Perform gene set enrichment analysis using fgsea on two conditions that don't have differential express genes
fgsea
20 days ago
Chris
▴ 10
1
vote
3
replies
238
views
Different Shrunk Log2FC according with reference
DESeq2
updated 26 days ago by
Michael Love
42k • written 26 days ago by
andrebolerbarros
▴ 20
3
votes
2
replies
238
views
Cannabis and KEGGgraph
KEGGREST
GenomicDistributionsData
KEGGgraph
KEGG
updated 28 days ago by
Guido Hooiveld
★ 4.0k • written 4 weeks ago by
fernanda.backsouza
▴ 10
1
vote
4
replies
233
views
minfi::read.metharray.exp
minfiData
minfi
updated 26 days ago by
Basti
▴ 780 • written 27 days ago by
clacarion
• 0
0
votes
3
replies
233
views
Couldn't connect to server while using "pathways"
graphite
updated 13 days ago by
Michael Love
42k • written 14 days ago by
fduguchunhui
• 0
2
votes
2
replies
232
views
fgsea significant result
fgsea
15 days ago • updated 14 days ago
Chris
▴ 10
0
votes
2
replies
229
views
PCA plot suggestions
DESeq2
PCAtools
updated 23 days ago by
Michael Love
42k • written 25 days ago by
Aaliya
• 0
0
votes
3
replies
227
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 18 days ago by
ATpoint
★ 4.1k • written 18 days ago by
james.zhang20
• 0
2
votes
5
replies
222
views
Error while installing BiocManager::install("org.Vdahliae.eg.db")
org.vdahlaie.eg.db
Bioconductor
4 days ago • updated 3 days ago
Patadù
▴ 10
1
vote
2
replies
216
views
Improving the design matrices vignette in RNAseq123
RNAseq123
28 days ago • updated 14 days ago
Lluís Revilla Sancho
▴ 730
0
votes
2
replies
215
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
18 days ago • updated 17 days ago
kcarey
• 0
1
vote
2
replies
205
views
Citation for edgeR user guide
edgeR
written 26 days ago by
k___
▴ 30
1
vote
1
reply
193
views
Handling multiple differential expression comparisons
DESeq2
updated 15 days ago by
Michael Love
42k • written 16 days ago by
Zainab
▴ 10
0
votes
2
replies
192
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 27 days ago by
taur.vil
• 0
0
votes
3
replies
190
views
Different number of exons in reference genome affects DEXSeq analysis
DEXSeq
updated 19 days ago by
Alejandro Reyes
★ 1.9k • written 4 weeks ago by
Alessia
• 0
0
votes
3
replies
188
views
ChAMP installation issue
Cha
ChAMP
7 days ago • updated 6 days ago
e1271094
• 0
2
votes
1
reply
181
views
minfi latest update for EPIC V2 chip
minfi
minfiDataEPIC
updated 13 days ago by
James W. MacDonald
65k • written 13 days ago by
e1271094
• 0
0
votes
1
reply
179
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 17 days ago by
Gordon Smyth
50k • written 17 days ago by
z_bahari
• 0
0
votes
1
reply
178
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 10 days ago by
ATpoint
★ 4.1k • written 10 days ago by
sajadahmad41454
• 0
169 results • Page
2 of 4
Recent ...
Replies
Comment: Numerical differences in DESeq2 output depending on different design matrix cons
by
Michael Love
42k
edgeR used a prior count which stabilizes LFC while DESeq2 does not. Note that the SE are larger in these cases so you don't have experime…
Comment: all variables in design formula must be columns in colData (Deseq2) error
by
Galina
• 0
Good day ATpoint, Could you pls advise also ehy this error could happened design contains one or more variables with all samples having t…
Comment: How to run allelic imbalance in apeglm
by
euge.eugenio
▴ 20
@mikelove could you help me? Thanks!
Comment: ENCORI or starBase : ceRNA data retrieval using Web API and curl
by
ATpoint
★ 4.1k
This is not related to Bioconductor. Biostars is more suited where you posted already.
Comment: all variables in design formula must be columns in colData (Deseq2) error
by
Galina
• 0
thank you!
Votes
biomaRt not working - lazy table error
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Comment: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Comment: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
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