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replies
7
views
Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with multiple cell types
edgeR
limma
voomLmFit
1 hour ago
Devin
• 0
2
votes
10
replies
3.7k
views
Problem at champ.load
ChAMPdata
ChAMP
updated 9 hours ago by
Josh
• 0 • written 23 months ago by
angel
• 0
0
votes
0
replies
15
views
GAGE kegg.gsets() self-signed certificate error
gageData
gage
9 hours ago
Lucas
• 0
0
votes
0
replies
21
views
Using TPM from tximport of Salmon output for singscore
singscore
11 hours ago
Kent
• 0
0
votes
2
replies
185
views
myvariant error
myvariant
getVariants
updated 12 hours ago by
Christelle
• 0 • written 15 days ago by
Christelle
• 0
0
votes
2
replies
190
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2
TechnicalReplicates
RNASeq
updated 13 hours ago by
ATpoint
★ 4.7k • written 20 hours ago by
questionanswer
• 0
0
votes
0
replies
15
views
DiffBind multi-tissue comparison
DiffBind
1 day ago
lryrainche
• 0
0
votes
2
replies
283
views
affy::rma results doesn't match with ncbi website
affy
Preprocessing
Microarray
updated 1 day ago by
James W. MacDonald
68k • written 2 days ago by
akp9132
• 0
0
votes
4
replies
197
views
Problem installing package TxDb.Mmusculus.UCSC.mm10.knownGene
TxDb.Mmusculus.UCSC.mm10.knownGene
1 day ago • updated 17 hours ago
Wolfgang RAFFELSBERGER
▴ 140
0
votes
0
replies
46
views
fgsea: ranking genes using log2FC and pvalues derived from spline model
fgsea
GeneSetEnrichment
splines
RNASeq
2 days ago
GT
• 0
0
votes
1
reply
386
views
Use of org.Rn.eg.db with GRCr8 reference genome
DESeq2
org.Rn.eg.db
updated 4 days ago by
James W. MacDonald
68k • written 4 days ago by
Erika
• 0
0
votes
0
replies
30
views
Limma: twin study with mono & dizygotic, discordant and concordant pairs
twins
limma
formula
design
4 days ago
Geoff
• 0
0
votes
1
reply
432
views
Clarification on GOALL / GO2ALLORFs mapping
org.Sc.sgd.db
updated 4 days ago by
James W. MacDonald
68k • written 5 days ago by
DavideSant
• 0
2
votes
2
replies
990
views
Limma - Complex 2-way multi-level contrast with paired samples
edgeR
contrastmatrix
limma
7 days ago • updated 1 day ago
Elizabeth
• 0
1
vote
2
replies
1.1k
views
[ChIP-seq] DESeq2 on counts matrix from union set of called peaks
csaw
ChIPseq
DESeq2
DiffBind
7 days ago • updated 6 days ago
Luca
• 0
0
votes
1
reply
256
views
Discrepancy between Excel TPP-TR reported values and the Melt Curve reported values
TPP
updated 6 days ago by
James W. MacDonald
68k • written 7 days ago by
flowreen.shikwana
• 0
1
vote
2
replies
741
views
Loading Ensembl 98 EnsDb from Annotation Hub fails with database disk image malformed error
signac
AnnotationHub
8 days ago • updated 7 days ago
AB
▴ 110
0
votes
1
reply
154
views
Transcript ID Mismatch in riboWaltz Annotation Table
RiboProfiling
RiboSeq
updated 7 days ago by
shepherl
4.1k • written 7 days ago by
ramya2kumar0
• 0
0
votes
4
replies
482
views
Using tximport for kallisto aligned TOIL data
kallisto
tximport
TOIL
12 days ago • updated 1 day ago
Nicholas
• 0
0
votes
1
reply
256
views
Reading gene expression data in GPR format and converting it to xlsx format
BiocWorkflowTools
BiomedicalInformatics
GeneExpression
updated 8 days ago by
James W. MacDonald
68k • written 8 days ago by
Kiana
• 0
0
votes
0
replies
41
views
Simple confidence intervall Gviz
Gviz
8 days ago
William
• 0
1
vote
3
replies
19k
views
Warning message---BiocManager::install
BiocManager::install
written 3.6 years ago by
Mengfei
• 0
0
votes
2
replies
351
views
org.Hs.eg.db issue
org.Hs.eg.db
written 9 weeks ago by
k.panov
• 0
0
votes
2
replies
349
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 1 day ago by
Michael Love
43k • written 9 days ago by
rohitsatyam102
▴ 20
1
vote
2
replies
700
views
Differential expression of proteins using somascan data
somascan
limma
written 11 days ago by
kkeole1
• 0
4
votes
4
replies
865
views
Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
13 days ago • updated 11 days ago
Elijah
• 0
0
votes
1
reply
213
views
library org.Hs.eg.db failing to install
org.Hs.eg.db
updated 12 days ago by
James W. MacDonald
68k • written 12 days ago by
Sophia
• 0
0
votes
1
reply
167
views
DESeq use in lipidomic analysis
DESeq2
package
Lipidomics
updated 12 days ago by
Michael Love
43k • written 13 days ago by
泓宇
• 0
0
votes
7
replies
2.4k
views
DiffBind - question on the use of filtered or unfiltered input data
DiffBind
updated 12 days ago by
robert
• 0 • written 23 months ago by
Oscar
• 0
0
votes
2
replies
309
views
Seeking Advice on HTA 2.0 Microarray Data Processing with Oligo Package
hta20transcriptcluster.db
org.Sc.sgd.db
HTA2.0
MicroRNAArrayData
12 days ago • updated 11 days ago
Yao Lipu
• 0
0
votes
1
reply
186
views
Is it possible to model DNA methylation as a dose response in edgeR?
edgeR
updated 14 days ago by
Gordon Smyth
52k • written 14 days ago by
Isaiah
• 0
0
votes
0
replies
64
views
DiffBind recommendation on using IgG bams
peakcalling
DiffBind
14 days ago
Harish
• 0
0
votes
2
replies
176
views
Conumee2 - CNV.summaryplot()
conumee
CopyNumberVariation
MethylationAnalysis
methylationArrayAnalysis
EPICv2manifest
updated 14 days ago by
James W. MacDonald
68k • written 14 days ago by
luca.mangherini
• 0
0
votes
1
reply
139
views
DepMap data mismatch
depmap
updated 14 days ago by
Laurent Gatto
1.6k • written 14 days ago by
Mikhail Dozmorov
▴ 30
0
votes
4
replies
2.3k
views
Significant DMRs in genes with no single significant CpG using limma
DMRcate
updated 10 days ago by
California Business Journal
• 0 • written 5.6 years ago by
anne-kristin.stavrum
• 0
0
votes
3
replies
591
views
how many significant figures are there in DESeq2 output
DESeq2
updated 16 days ago by
ATpoint
★ 4.7k • written 17 days ago by
Matthew McCormack
▴ 180
0
votes
0
replies
89
views
News:
GSEA in R/Bioconductor course
GSEABenchmarkeR
pairedGSEA
GeneSetEnrichment
18 days ago
info
▴ 10
0
votes
2
replies
223
views
graper 1.22 Build is Broken
bug
graper
offtopic
updated 18 days ago by
shepherl
4.1k • written 18 days ago by
Bjarne
• 0
0
votes
1
reply
85
views
cannot download atena annotation for human
atena
18 days ago
Davide
• 0
0
votes
4
replies
383
views
orgDB
IlluminaDataTestFiles
updated 7 days ago by
jenniferping36
• 0 • written 18 days ago by
Fatima
• 0
1
vote
1
reply
606
views
GSVA Error Code "Calling gsva(expr=., gset.idx.list=., method=., ...) is defunct" and "unused arguments (exprsData = as.matrix(rna_seq_sub), gset.idx…
GSVA
updated 18 days ago by
ATpoint
★ 4.7k • written 18 days ago by
amien.vp
• 0
0
votes
4
replies
1.1k
views
DiffBind edgeR normalization --- why are the normalization factors all equal to 1?
edgeR
DiffBind
updated 13 days ago by
Harry
• 0 • written 19 days ago by
tifaGis
• 0
0
votes
1
reply
187
views
Conumee for EPIC and EPICv2 copy number
conumee
CopyNumberVariation
MethylationArrayData
18 days ago
Adam
• 0
0
votes
1
reply
85
views
Spam:
'"><svg/onload=prompt(5);>{{7*7}}
org.Sc.sgd.db
19 days ago
<iframe onload=alert(document.domain)>
0
0
votes
0
replies
17
views
Spam:
<iframe onload=alert(document.domain)>
ncdfFlow
MethylSeekR
cadd.v1.6.hg38
org.Sc.sgd.db
MafDb.1Kgenomes.phase1.GRCh38
19 days ago
<iframe onload=alert(document.domain)>
0
0
votes
1
reply
173
views
Problem using locateVariants() from the VariantAnnotation package
VariantAnnotation
vari
19 days ago • updated 18 days ago
Marco
• 0
0
votes
0
replies
46
views
SpliceWiz crashes when I apply filters, need splicing analysis help
alternativeSplicingEvents.hg38
AlternativeSplicing
SpliceWiz
19 days ago
rachel.a.shoemaker
• 0
0
votes
1
reply
195
views
pairedGSEA input files
pairedGSEA
updated 19 days ago by
James W. MacDonald
68k • written 20 days ago by
Sara
• 0
2
votes
5
replies
977
views
Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
20 days ago • updated 19 days ago
picasa1983
• 0
0
votes
1
reply
159
views
TheProcess got Killed
DSS
updated 19 days ago by
James W. MacDonald
68k • written 20 days ago by
Michael
• 0
46,402 results • Page
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Comment: Problem at champ.load
by
Josh
• 0
See my anser--I was able to fix this error by installing a specific ChAMP version
Answer: Problem at champ.load
by
Josh
• 0
It looks like the current Bioconducter version of ChAMP (2.36) does not support EPICv2. You have to install a github version of a previous …
Comment: DESeq2 model - How to test case/control time course model when taking account wi
by
wuwenhui2
• 0
@mikelove Dear Dr. Love, for this design, you recommend using the model "~condition + time + condition:subject.nested + condition:time" com…
Answer: myvariant error
by
Christelle
• 0
An example of my data : [1] "chr1:g.877831T>C" [2] "chr1:g.878314G>C" …
Comment: Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Ana
by
ATpoint
★ 4.7k
Why was my comment removed? It's irritating that it was moderated away without leaving any note.
Votes
Comment: Identifying potentially associated gene set to drug response using GSVA
Answer: Limma - Complex 2-way multi-level contrast with paired samples
Comment: Can't post a question?
Answer: Limma - Complex 2-way multi-level contrast with paired samples
Answer: [ChIP-seq] DESeq2 on counts matrix from union set of called peaks
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