26,437 results • Page 2 of 441
Dear all, I'm using DiffBind to find the differentially methylated regions. Libraries have been obtained by using the Methyl-binding...Should I go for the background or the Reads in Peak (RiP) normalization? Are there some specific "rules" for the MBD-seq? I know, and it is clearly written in the manual, that there are many strategies and none is the perfect one
updated 16 months ago • Marianna
Hello all, I've been trying to install WGCNA on our cluster. It has module system, so I've chosen R-3.1.2 and installed bunch of libs locally...a>") biocLite("GO.db") </pre> <pre> it returned ----- (download, installation logs here, no errors) --------- ** testing if installed package can be loaded Error in sqliteSendQuery(con, statement, bind.data) : error in state…
updated 10.1 years ago • predeus
div class="preformatted">Hi Diana, the 'issue' you are seeing is due to the "True Path Rule". I suspect that the geneID2GO mappings gives only the specific annotation to a particular GO term. However during the...are pushed up the hierarchy. So a GO term will contain the genes specifically annotated to it plus all the genes present in the children (and it goes recursively). The root of the h…
updated 12.4 years ago • Adrian Alexa
Hi all, anyone can help with the following from the ATACseqQC package: objs &lt;- splitBam(bamfile, tags=tags, outPath=outPath, + txs...txs, genome=genome, + conservation=phastCons100way.UCSC.hg19) ERROR as follows: &gt; [E::sam_parse1] unrecognized type N &gt; [W::sam_read1] Parse error at line 26 &gt; [E::sam_parse1] u…
updated 6.7 years ago • theodore.georgomanolis
div class="preformatted"> Apple MAC OS X 10.4 R version 2.3.1 Running biocLite() with groupName = "all" does yield an OS error "/Applications/R.app/Contents/Resources/sush: pipe error: Too many open files" Is it possible to close...files as the installation proceeds? &gt; biocLite(groupName = "all", destdir = "/Volumes/KilroyHD/kilroy/Software/BioConductor") Running getBioC version 0.1.…
I would like to use R. I have seen some annotation packages for R but specifically I need ACMG rules for my SNPs. Is there a way to do it in R? Is there any Bioconductor packages that gives ACMG rules as annotations? Thank you
updated 3.5 years ago • hamza_karakurt
Hi all, Would you have a suggestion how to fix this error? peak.counts &lt;- regionCounts(pe.bams, all.peaks, param=param) Error: BiocParallel...errors 1 remote errors, element index: 1 3 unevaluated and other errors first remote error: Error: position vector should be...1-based I found a similar error here: [https://support.bioconductor.org/p/9144941/#9148614]…
updated 2.8 years ago • Chris
div class="preformatted"> hi all i'm using the EBcoexpress package to analyze a series of 29 Tumor vs 29 Normal cancer microarray data. following the directions...in the pdf file i can create the first coexpression matrix with no errors, however, when i run the initializeHP() function (as decscribed in the package file) i get the following error: Error in mvnX...data = data, prior = prior):…
I am one of the managers of a computing system that has a very restrictive set of outbound firewall rules but that does allow access to https://bioconductor.org/. Recently, one of our users reported an error accessing URL https...https://mghp.osn.xsede.org/ is not in our set of allowed external sites, that explains the user's error. Although we were able to provide this user a workaround, I want …
updated 3.4 years ago • ems
div class="preformatted">Hello, Is there a way to get all genes' info in the SAM result table? sam.out &lt;- sam(samples.mat,cl,method="d.stat",rand=123,p0=0.95); sum.table&lt;-summary(sam.out...0,ll=F,file = "result.csv",sep=",",quote=F); Error in stats.cal(object at d, object at d.bar, object at vec.false, object at p0, : delta must be larger than 0. If I put the delta...number…
updated 17.7 years ago • wxu@msi.umn.edu
hi , &nbsp; When running the search for all promoters sequences&nbsp; in mice , with&nbsp; 1000 bp&nbsp; up /downstream, parameter the getPromoterSeq&nbsp; works fine...but increasing the up/downstream parameter to say 5000&nbsp; or 2000 it errors&nbsp; (log bellow): Any advice appreciated . Branko ------------------------------------------- <pre> libra…
updated 11.2 years ago • branislav misovic
div class="preformatted"> I am finding a new error in getBioC using R for Windows v1.6.1. I am getting error message. &gt; getBioC() [1] "Getting/installing reposTools and Biobase...Error in FUN(X[[1]], ...) : couldn't find function "note" And after trying the suggested (libName="all")-- the same. &gt; getBioC(libName="all") [1] "Getting...installing reposTools and Biobase" Error in…
updated 23.2 years ago • Stephen Henderson
<div class="preformatted">Dear Bioconductors, I am working with an array containing 17496 probes spotted in duplicate. To deal with duplicate spots I used duplicateCorrelation like this: cor&lt;-duplicateCorrelation(MA,ndups=2, spacing=1458) This results in a warning message: Warning message: Too much damping - convergence tolerance not achievable in: glmgam.fit(dx, dy, s tart = s…
updated 20.9 years ago • Georg Otto
analyze my RNASeq data with DESeq2. When trying to import files via tximport, I get the following error: ``` txi &lt;- tximport(files, type="salmon", tx2gene=tx2gene) Error in tximport(files, type = "salmon", tx2gene = tx2gene) : all(file.exists...issued by others I checked on my file path und directory, both are correct. - When performing: all(file.exists(files)) I receive [1] FAL…
colData = colData2, design = ~ condition) but I am getting this error: Error in DESeqDataSet(se, design = design, ignoreRank) : all variables in design formula must be columns in colData I checked
updated 3.7 years ago • Maryam.sed1400
data, and I've encountered an issue while attempting to plot the Total Ion Chromatogram (TIC) for all of my samples. Strangely, I was able to accomplish this task effortlessly in the past, but for some reason, I'm now facing difficulties...underlying cause. Your assistance in resolving this matter would be greatly appreciated. The error is : Error: BiocParallel errors 1 remote errors, elemen…
updated 2.4 years ago • mavamay
class="preformatted">Dear Group I am trying to plot a simple a scatter plot using ggplot, I removed all NA and all rows with zero variance as well but I am still getting the error below: ggplot(df aes(x=df[,1], y=df[,2])) + + geom_point(shape...line + theme(axis.text=element_text(size=1)) # (by default includes 95% confidence region) Error in seq.default(from = best$lmin, to = best$lma…
updated 12.0 years ago • Alyaa Mahmoud
Hi, I am encountering an error when trying to run the read.flowSet. I have a folder of .fcs files from a 96 well plate, one file per well. I concatenate the...info into a dataset, but recently I have started to have errors when running my code, which is due to the read.flowSet command returning Error: vector::\_M\_range\_check. The only option...I am loosing a lot of data doing so. It is notew…
0 1 fdee8bef.5ce2.4ca6.b6b9.3423219a1ea4 0 1 # I get an error when I try to apply voom: &gt; voom(tmp, design = design) Error in approxfun(l, rule = 2) : need at least two non-NA values to interpolate...pre> I searched for the error and I found that it is usually encountered when you have no replicates. However in my case I have enough replicates - st…
updated 9.4 years ago • komal.rathi
Hi, I've seen many posts in relation to my question but they all included replicates. My sample file looks like this (had to add bullet points so was readable and not inline): - run time sample...1 0 1 - 2 2 2 - 3 6 3 - 4 8 4 I want to compare all of them against baseline so time 0 and then pull the results table after running DESeq2 with all the contrast 0vs2, 0vs6...and 0v…
updated 6.3 years ago • luciest.rodriguez
preformatted">Hi, I'm a little stuck with the charm analysis using MeDip arrays. It started with an error at "readCharm" and trickled into the finer details of what information the function needed. I have seen the previous...X Y DMD 537151_1_25 BLOCK1 interval rank target_tm:76.00;probe_tm:77.60;freq: 9.25;count:01;rules:0000;score:0812 chr19:6588109-6599163 AAAAGACCAGAAAACAG GGCACGGACGTAAGC…
div class="preformatted"> Hi all, I have been working with minfi and am trying to use the code for dmp finder. I am through with all the steps till now, but I am...M &lt;- getM(mset, type = "beta", betaThreshold = 0.001) On doing the above, I get the following error: Error in getM(mset, type = "beta", betaThreshold = 0.001) : unused argument(s) (betaThreshold = 0.001) Can anyone help…
updated 13.2 years ago • Guest User
Hi, When I try to calculate the overlap rate between peaksets, I get the following errors: &gt; names(broad_gtsf1$masks) # just list all [1] "K562" "H3K9Me3" "75" "313" "WT" "Mutant" "counts" "Consensus" "Replicate.1" "Replicate.2...Hi, When I try to calculate the overlap rate between peaksets, I get the following errors: …
updated 6.6 years ago • aviv.de-morgan
Hi,all I run deseq2 on Ubuntu 20.4 but I get this error. I Would be appropriate it to help me. CMD: R --no-save --no-restore --no-site-file --no...2_vs_SLRed.SH-2.vs.SLRed.Deseq2.Rscript /home/mah/miniconda3/lib/R/bin/exec/R: error while loading shared libraries: libreadline.so.6: cannot open shared object file: No such file or directory Error, cmd
updated 3.5 years ago • Mahsa
it. It is possible to take output form Cufflinks - transcripts.gtf and extract FPKM value for all genes and do sum of all FPKM's? Something like - awk 'OFS="t"{sum+=$16}END {print sum}' transcripts.gtf. And also when I print in R command...I am little desperate :-( Kind Regards, Petr. -- output of sessionInfo(): &gt; session(info) Error: could not find function "session" &gt; sess…
updated 11.7 years ago • Guest User
Hi, I would like to do differential expression analysis of one group of samples (miRNA) against all samples in the dataset (e.g samples A,B,C, against samples A...K ). At the moment I have tried to use DESeq2 for this purpure. I did...not find description of such possibility in DEseq2 manual, however it did not give me any errors while I was doing that. My question is if it is OK to use DESeq2 i…
updated 9.3 years ago • ofonov
use detectTranscripts() from groHMM using bams from a PRO-seq experiment. I am getting the following error: <code>Error in Fp[[i]] + 1 : non-numeric argument to binary operator<br/> In addition: Warning messages:<br/> 1: In mclapply(readsList...function(x) { :<br/> &nbsp; all scheduled cores encountered errors in user code<br/> 2: In mclapply(readsList, func…
updated 7.5 years ago • warrena
Hey Adi, i have used spia a copule of time now and i never got this error before but just recently i am having this error, all my data looks very fine to me at every step but when i try running spia...i get this error message.Although my DE-Colorectal has both the entrez ID and the logfoldchange in it. Error in spia(de = DE\_Colorectal...all = ALL\_Colorectal, organism = "hsa",&nbsp; : &…
updated 9.6 years ago • saamar.rajput
Hi all, I am using R package ReactomePA for enrichment analysis. But I am getting this type of error after reading each command...perfectly also. Error in x\[seq\_len(n)\] : object of type 'S4' is not subsettable Thanks! Any help
updated 9.5 years ago • Ram
Dear all, it turns out I cannot use biomaRt anymore. do you have any clue or know from what it might depend? ensembl_db &lt;- useEnsemblGenomes...biomart = "plants_mart") Error in `collect()`: ! Failed to collect lazy table. Caused by error in `db_collect()`: ! Arguments in `...` must be used. Problematic argument...1 = Inf Did you misspell an argument name? Run `rlang::las…
updated 24 months ago • goldenboy
asking me if I want to update some other packeges. How can I force the answer to be always "update all" without having to answer? source("http://bioconductor.org/biocLite.R") biocLite("Biostrings") [...] Update all/some/none? [a/s/n
updated 6.9 years ago • rgiannico
div class="preformatted"> Hi, first, thanks to you all for the help... I made my own package, using ABprimer.pdf vignette first and then using AnnBuilder.pdf. I didn't receive errors...packages looks like correctly created with all subdirectories etc etc... To install the packages (windows OS) I either copied the dirs and the subdirs in the R library directory...the "test" package, but with …
updated 20.9 years ago • Giulio Di Giovanni
This is a question on the dba.report function in DiffBind. I am trying to retrieve all sites (including non-differential and differential sites) by using the following script: GRanges.steady &lt;- dba.report...diff.analysis.steady, method=DBA\_DESEQ2\_BLOCK, th=1, bUsePval=FALSE) However, the following error message was returned: Error in .Call2("solve\_user\_SEW0", start, end, width, PAC…
updated 10.7 years ago • meisan406
I've performed my DEseq2 analysis on my samples. However, when I take a look at the resulting output, all of my p-adjusted values are the same for all genes and are equal to 0.9999068. Does this mean I have created an error upstream
updated 4.0 years ago • bjen731
ignoreRank = FALSE) # include your problematic code here with any corresponding output #Error in DESeqDataSet(se, design = design, ignoreRank) : all samples have 0 counts for all genes. check the counting script. # please...adding a column with row numbers (using the rowid_to_column) function but it returned the same error message. Anyone help?? I've going round and round for day…
updated 4.3 years ago • Sonia
Hi, All I am new to Rstudio , i Have to study expression data for the following But my DESeq2 data is having an error of Error in h(simpleError...msg, call)) : error in evaluating the argument 'x' in selecting a method for function 'ncol': object 'countData' not found It would be helpful
updated 4.1 years ago • mithu18mohan
errors in user code, all values of the jobs will be affected 5: In mclapply(x, "\[\[", j, mc.cores = cores) : &nbsp; scheduled cores 4 encountered...errors in user code, all values of the jobs will be affected 6: In mclapply(x, "\[\[", j, mc.cores = cores) : &nbsp; scheduled cores 4 encountered...errors in user code, all values of the jobs will be affected 7: In mclapply(x, "\[\[", j…
updated 9.1 years ago • Sylvain Foisy
I'm trying to set up a DEG analysis with deseq2. I've been getting an error running&nbsp;DESeqDataSetFromMatrix and I haven't been able to figure out the source from the error message. <code>&gt...Dose+Compound &nbsp; &nbsp; #must match testConditon<br/> + &nbsp; &nbsp; &nbsp; &nbsp; )<br/> Error in DESeqDataSetFromMatrix(countData = countdata,…
updated 9.3 years ago • lauren.fitch
div class="preformatted">Hi All any one encountered this error before ? I have the following error when using pvlcust for a transposed matrix. Please advise...pv &lt;- pvclust(as.matrix(t(bb)), method.dist="correlation", method.hclust="complete", nboot=10) Error in solve.default(crossprod(X, X/vv)) : Lapack routine dgesv: system is exactly singular In addition: There were 11 warnings.…
updated 13.6 years ago • Alyaa Mahmoud
Hi to all, Simple question for DiffBind that isn't clear to me despite a lot of searching and (over) thinking. How exactly is the count...scaled counts? &nbsp; Thank you for any responses! Bonus question: I realize there's no hard rules and you would need data to make a real suggestion, but is simply scaling counts by library-size be considered sufficient...normalization or would you r…
updated 9.0 years ago • siklenkak
directory '/tmp/Rtmp5DxR9W/R.INSTALL42134b7bec9/lpsymphony/src/SYMPHONY/CoinUtils' Making all in Osi make[2]: Entering directory '/tmp/Rtmp5DxR9W/R.INSTALL42134b7bec9/lpsymphony/src/SYMPHONY/Osi' Making all in src...407: all] Error 2 make[3]: Leaving directory '/tmp/Rtmp5DxR9W/R.INSTALL42134b7bec9/lpsymphony/src/SYMPHONY/Osi/src/Osi' make[2]: *** [Makefile...532: all-recursive] Error 1 make…
updated 3.9 years ago • starsareintherose
Hello! I am new to both RSEM and tximport, so I am unsure which this is an issue with. All of my RNA seq samples are in separate SampleName.rsem.genes.results files in one directory. Based on the vignette, it...almost seems like all the samples should be in one gene.results file? Either way, I attempted to pack the files and read them in via the below code...genes.results") txi.rsem=tximport(rse…
updated 8.2 years ago • hs.lansdell
<div class="preformatted">Dear all, I am getting error when i try to read .bam files. The problem is that i don't understand what does not it mean and what I do to solve it. here's the error. " Error in queryHits(findOverlaps(query, subject, maxgap = maxgap, minoverlap = minoverlap, : error in evaluating the argument 'x' in selecting a method for function 'queryHits': Error in (functio…
updated 12.6 years ago • Reema Singh
example given in the trackViewer vignette but when I alter the code to plot fut3 variants I get this error, "Error: all(width(SNP.gr\[\[i\]\]) == 1) is not TRUE" &nbsp; Below is the code from the trackViewer vignette that works fine. <pre> &gt; library...IRanges(5842040,5852344, names="FUT3")) &gt; bgzip( "F:/WT 18 month project/Analysis/fut3.popvcf") Error in .zip(.bgzip,…
updated 8.1 years ago • rop.jesse
<div class="preformatted">hi i get an error when trying to install R 2.9.1 from source on an intel mac os 10.5.7. i have used the gcc4.2 from xcode 3.1 and gfortran 4.2 from http://r.research.att.com/tools/. i also tried using gcc and gfortran 4.3 installed with macports. same error. im not an expert in this and im a bit lost as to the "source" of my problem? here follows some of the out…
updated 16.5 years ago • Jesper Ryge
Dear all,&nbsp; &nbsp; I have a trouble with noGGi. When I tried to run "regionPlot" command using bam and bed files, a error occurred after...finishing reading Bam files. The error message is the below: "Error in&nbsp;Filtering regions which extend outside of genome boundaries... (function (classes, fdef
I am currently working in plant pathogen interaction and is trying to make a gene co-expression network using RNA-seq data. I used HTseq-count to get the read counts. My dataset consist of Resis_treated (1st day, 3rd day and 7th day) samples taken under 3 time point with 2 replicated for each time point. I have a factor with 3 levels condition Day1 Day1 Day3 Day3 Day7 Day7 I am…
updated 6.6 years ago • aishu.jp
Dear all, Our experiments is based on gene expression of a single tissue with contrasts between 3 treatments of 3 replicates. Each...indicate a tiny amount of fold-changes for the majority of genes in the experiment; this is true for all three possible contrasts for that species. MA plots look normal for the other species. We are trying to identify or rule
updated 6.8 years ago • Jack.Hearn
<div class="preformatted">Hi all, just a quick question on best practices to create datasets for RNA-seq analysis with edgeR or limma:::voom(). I must create a...div class="preformatted">Hi all, just a quick question on best practices to create datasets for RNA-seq analysis with edgeR or limma:::voom(). I must create...In order to do this I have at least a couple of options that may affe…
updated 13.5 years ago • Cittaro Davide
Hello, I have read all comments about the following error comes from GOstat package,&nbsp; <pre> &gt; res &lt;- summary(hgOver,pvalue=hgCutoff) Error...in .checkKeys(value, Lkeys(x), x@ifnotfound) : value for "GO:0098856" not found Error during wrapup: cannot open the connection</pre> <pre> &gt; traceback() No traceback available </pre> Almos…
Hi, I want to do pathway analysis with "ReactomePA" in R, I installed all dependencies but I get this error: Error : Functions found when exporting methods from the namespace ‘S4Vectors’ which are...not S4 generic: ‘grep’, ‘grepl’ Error: package or namespace load failed for ‘DOSE’ &nbsp
updated 8.8 years ago • niutster
with 2 columns with protein product and locus tag taken from NIH. However i am getting the following error when running the command for tximport ```txi &lt;- tximport(files, type="salmon", tx2gene=tx2gene)``` error: ```reading in files with...read_tsv 1 Error in tximport(files, type = "salmon", tx2gene = tx2gene) : all(c(abundanceCol, countsCol, lengthCol) %in% names(raw)) is …
updated 2.7 years ago • Joana
div class="preformatted">Hi, I got an error in annaffy function that I use a lot. I installed R 2.5 and all BioC package but still got the same error anntable &lt;- aafTableAnn
updated 18.7 years ago • Mayte Suarez-Farinas
<div class="preformatted">Hello there, I am writing for a second time to ask for some help with a LimmaGUI error I am having. I am trying to load some BlueFuse results files into LimmaGUI. I am able to load the data in (seen in R window as...preformatted">Hello there, I am writing for a second time to ask for some help with a LimmaGUI error I am having. I am trying to load some BlueFus…
according to the steps in the documentation. However, when trying to analyze the champ.block, I get errors, and from some reason - a different error in each run. My data includes 37 samples analyzed by EPIC array. All the analyses...lt;- champ.Block(beta=myNorm,pheno=myLoad$pd$Sample\_Group,arraytype="EPIC") And here are the error lines: Error in names(revOrder) &lt;- seq\_along(ret$idx) :…
updated 8.1 years ago • tiroshamit
div class="preformatted">Hi all, If I have a 2x2 design (gen[AB], treat[CD]), I've created a design matrix so that I have 4 classes: A.C, A.D, B.C and B.D. Now I'm interested...CF: 03 06 80 153 info:www.5xmille at hsr.it - www.5xmille.org Disclaimer added by CodeTwo Exchange Rules 2007 http://www.codetwo.com </div
updated 13.4 years ago • Cittaro Davide
Hi,&nbsp; I have a problem with a script. It gives error at the very beginning at I really don't know why. I used the exact same one few months ago and it was working absolutely...Hi,&nbsp; I have a problem with a script. It gives error at the very beginning at I really don't know why. I used the exact same one few months ago and it was working absolutely fine...the analyses and I can't…
updated 8.6 years ago • sil1
code sigCpGs &lt;- DMPs$Name[DMPs$adj.P.Val&lt;0.05] length(sigCpGs) [1] 311082 all &lt;- DMPs$Name length(all) [1] 870529 par(mfrow=c(1,1)) gst &lt;- gometh(sig.cpg=sigCpGs, all.cpg=all, plot.bias=TRUE) I got following...error All input CpGs are used for testing. Error in getMappedEntrezIDs(sig.cpg = sig.cpg, all.cpg = all.cpg, array…
Hi, all I'm trying to get some snp information, such as ID, position, etc., from biomaRt. The code I'm using is attached below: <pre> &gt; require...chr_name', values=22, mart=dataset)</pre> But after hours of waiting, I always get the same error message: <pre> Error in scan(file, what, nmax, sep, dec, quote, skip, nlines, na.strings, : line 545406 did not have 6 …
updated 11.2 years ago • Ting
Hi all: I am trying to analyze zebrafish ATAC seq data using DiffBind. When I run dba.count I get an error like this. Error: Error processing...Warning messages: 1: In mclapply(arglist, fn, ..., mc.preschedule = TRUE, mc.allow.recursive = TRUE) : all scheduled cores encountered errors in user code 2: error in evaluating the argument 'x' in selecting a method for function...assay': Bi…
updated 4.0 years ago • Ayyappa
26,437 results • Page 2 of 441
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