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Showing :
Limma
•
reset
0
votes
11
replies
2.3k
views
limma::contrast.fit() potential bugs when weights from voom() included
bugs
limma
3.2 years ago
Zhenyu
• 0
2
votes
3
replies
1.2k
views
limma::goana - "Invalid value for odds"
limma
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
neilzhao
▴ 30
0
votes
0
replies
879
views
Finding DEGs between two different datasets using limma
Proteomics
limma
proteomi
3.2 years ago
mfaleevs
• 0
5
votes
6
replies
2.5k
views
Selecting individual samples from tximport and DGE matrix design with missing biological replicate
DESeq2
limma
tximport
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
Kent
• 0
0
votes
0
replies
799
views
Tuning the MetaVolcanoR plots
limma
MetaVolcanoR
3.2 years ago
JAcky
• 0
1
vote
10
replies
3.9k
views
multiple voom runs with duplicateCorrelation and negative values in limma
limma
voom
3.2 years ago
Hilary
▴ 40
0
votes
3
replies
1.5k
views
Design and contrast matrices for analyzing the effect of two factors of interest on protein expression, controlling for factors and covariates not of…
limma
Proteomics
ProteomicsWorkflow
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
samantha.v.goncalves
• 0
2
votes
2
replies
1.1k
views
paired samples, comparing expression given a continuous variable
limma
updated 3.3 years ago by
Gordon Smyth
53k • written 3.3 years ago by
naz
• 0
1
vote
1
reply
879
views
ordinal treatment group design / contrast matrix
limma
updated 3.3 years ago by
James W. MacDonald
68k • written 3.3 years ago by
Chris
• 0
0
votes
1
reply
1.4k
views
Building model matrix to correct for batch effect with biological and technical replicates: Problem with linear correlations
MassSpectrometryData
BatchEffect
r
limma
updated 3.3 years ago by
Gordon Smyth
53k • written 3.3 years ago by
mfaleevs
• 0
1
vote
1
reply
922
views
IS DGE possible with normalized microarray expression data and Limma ?
limma
MicroarrayData
updated 3.3 years ago by
James W. MacDonald
68k • written 3.3 years ago by
waltheja
• 0
2
votes
2
replies
2.0k
views
How to judge DEG analysis of these samples could be done by using limma::voom
limma
Clustering
voom
RNA-Seq
3.3 years ago
Yang Shi
▴ 10
2
votes
4
replies
2.1k
views
pre-ranked GSEA or not?
log2FC
DESeq2
GSEA
voom
limma
updated 3.3 years ago by
Gordon Smyth
53k • written 3.3 years ago by
Yang Shi
▴ 10
5
votes
6
replies
2.7k
views
Removing the duplicated genes before limma::voom by using limma::avereps
RSEM
avereps
voom
limma
RNASeq
3.3 years ago
Yang Shi
▴ 10
2
votes
4
replies
2.7k
views
Doing normalization when using voom for DEG analysis?
limma
RSEM
voom
Normalization
3.3 years ago
Yang Shi
▴ 10
0
votes
0
replies
946
views
MetaVolcanoR usage
limma
MetaVolcanoR
EnhancedVolcano
3.3 years ago
JAcky
• 0
3
votes
4
replies
1.5k
views
Can I use limma::avereps to remove dupicated genes before conduct DEG based on RSEM Expected_count data?
limma
RNASeq
Expected_count
DESeq2
3.3 years ago
Yang Shi
▴ 10
1
vote
1
reply
1.0k
views
Weight Array Quality before of after applying variance filtering?
limma
arrays
genefilter
updated 3.3 years ago by
James W. MacDonald
68k • written 3.3 years ago by
jacorvar
▴ 40
2
votes
6
replies
2.0k
views
Limma beta coefficient confidence intervals
limma
limmaGUI
beta
3.4 years ago
fluentin44
• 0
0
votes
1
reply
1.2k
views
Compare different cell lines with tissues using limma
limma
MicroarrayData
Microarray
Agilent
updated 3.4 years ago by
Gordon Smyth
53k • written 3.4 years ago by
Sam
• 0
3
votes
9
replies
2.9k
views
Help with limma contrast, difference of a difference?
limma
3.4 years ago
Ahdee
▴ 60
0
votes
8
replies
2.2k
views
Finding differentially expressed genes in a prospective cohort study
limma
MicroarrayData
Microarray
3.4 years ago
YC
• 0
0
votes
3
replies
3.1k
views
Problem in kegga() and others getting data from KEGG after switch from http to https
limma
KEGGREST
EGSEA
updated 3.4 years ago by
shepherl
4.2k • written 3.4 years ago by
Jenny Drnevich
★ 2.0k
0
votes
2
replies
949
views
Get confused about the mean log-intensity (Amean)
limma
3.4 years ago
OE
• 0
2
votes
9
replies
4.0k
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA
r
RNASeqData
voom
limma
3.4 years ago
Peipei
• 0
1
vote
8
replies
2.3k
views
edgeR design matrix with multiple cell types and combination treatments
DifferentialExpression
celltype
treatment
edgeR
limma
3.4 years ago
giBio
• 0
0
votes
2
replies
1.5k
views
How do I remove the outlier probes highlighted in the plotSA function of limma
limma
updated 3.4 years ago by
Gordon Smyth
53k • written 3.4 years ago by
S
• 0
1
vote
2
replies
1.3k
views
Problem with results of Differential expression in Affymetrix microarray
limma
MicroarrayData
Microarray
Affymetrix
3.4 years ago
Mikołaj Mierzejewski
• 0
1
vote
2
replies
1.8k
views
Affymetrix analysis of microarray by using Limma
limma
MicroarrayData
Microarray
Affymetrix
3.4 years ago
Mikołaj Mierzejewski
• 0
2
votes
1
reply
2.1k
views
removeBatchEffect: Coefficients not estimable when using "batch matrix" for `covariates` argument.
limma
BatchEffect
updated 3.4 years ago by
Gordon Smyth
53k • written 3.4 years ago by
nhaus
▴ 70
6
votes
2
replies
3.2k
views
Pseudo-bulk Limma Approaches
limma
updated 3.4 years ago by
Gordon Smyth
53k • written 3.5 years ago by
andrew.j.skelton73
▴ 370
9
votes
3
replies
9.6k
views
Limma vs deseq2 results
limma
RNASeq
R
3.5 years ago • updated 3.4 years ago
k___
▴ 30
2
votes
2
replies
1.4k
views
Help with constructing design matrix for edgeR/limma
limma
design.matrix
model.matrix
edgeR
3.5 years ago • updated 3.4 years ago
adrianna_07
• 0
1
vote
4
replies
1.5k
views
How to figure out the FC value from Limma
limma
DEGseq
DifferentialExpression
updated 3.5 years ago by
Kevin Blighe
★ 4.0k • written 3.5 years ago by
beslinail
• 0
1
vote
1
reply
1.4k
views
Limma for paired and unpaired samples. Strange number of DEGs in unpaired samples
limma
lmfit
R
DifferentialExpression
updated 3.5 years ago by
ATpoint
★ 5.0k • written 3.5 years ago by
Dimitris
• 0
3
votes
3
replies
2.4k
views
Question on RNA-Seq single sample data analysis in EdgeR
R
edgeR
limma
RNASeq
Normalization
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
mohammedtoufiq91
▴ 10
0
votes
6
replies
2.0k
views
regression analysis for epic array methylation data using limma
limma
Epigenetics
STATISTICS
R
3.5 years ago
Jitendra
▴ 10
0
votes
2
replies
1.6k
views
Using limma to read Illumina HT-12 v4 data
limma
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
orbitofrontal
• 0
4
votes
6
replies
2.3k
views
How to best visualize multi-level transcriptomics experiment data?
ggplot2
Transcriptomics
Visualization
R
limma
3.5 years ago
mohammedtoufiq91
▴ 10
3
votes
4
replies
2.7k
views
Quantile Normalization after rma or not?
limma
Microarray
oligo
3.5 years ago
Yang Shi
▴ 10
2
votes
2
replies
2.6k
views
Handling NA values in limma's voom design matrix
limma
voom
design
NAs
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
dr
▴ 10
1
vote
2
replies
1.5k
views
toptable sort .by set
limma
rnaseqGene
3.5 years ago
snowmattin
• 0
1
vote
5
replies
3.1k
views
Unsuccessful DE analysis using limma - voom
limma
3.5 years ago
JAcky
• 0
2
votes
1
reply
1.6k
views
Extremely low P-values when using Limma on high sample size proteomics data
limma
Proteomics
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
roger.moreno.justicia
• 0
2
votes
1
reply
1.1k
views
Limma model orientation
limma
methylation
MethylationArray
updated 3.5 years ago by
James W. MacDonald
68k • written 3.5 years ago by
fluentin44
• 0
2
votes
4
replies
1.8k
views
Overfitting in limma
limma
Regression
overfitting
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
Marek Gierlinski
▴ 30
0
votes
0
replies
785
views
diffcyt - topTable function returns one total significance estimate instead of one per comparison
limma
diffcyt
edgeR
3.5 years ago
stefanos.bamopoulos
• 0
2
votes
4
replies
5.1k
views
Limma: How to deal with multiple batch effects and what is going on under the hood
limma
voom
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
jmannhei
▴ 20
0
votes
3
replies
1.0k
views
Limma time course with several conditions
limma
3.5 years ago
rina
▴ 30
0
votes
7
replies
1.9k
views
Which Limma guide is recommended ?
limma
R
DifferentialExpression
3.6 years ago • updated 3.5 years ago
JAcky
• 0
4,225 results • Page
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Comment: What R/Bioconductor tools would you recommend for the analysis of sncRNA, specif
by
Matthew Thornton
▴ 380
WOW! Thank you so much for the information. I will do this.
Comment: "Watched Tags" are being erased rather than updated
by
RalphSavage99
• 0
Thanks for sharing https://github.com/Bioconductor/support.bioconductor.org/issues/112
Answer: Add additional maintainer for MOFA2
by
shepherl
4.2k
These requests are best on the bioc-devel @ r-project.org or a request to maintainer @ bioconductor.org -- We would ideally need the curre…
Answer: After running samtools, the featureCounts command crashed.
by
Kevin Blighe
★ 4.0k
Hi Rui Tang, The "buffer overflow detected" error in featureCounts usually stems from a corrupted or malformed GTF file, often due to tr…
Answer: Problem with the TMB plot
by
Kevin Blighe
★ 4.0k
Hi, The linear TMB plot with no clustering suggests your variant calls may lack sample-specific discrimination, likely due to filtering ap…
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Answer: What R/Bioconductor tools would you recommend for the analysis of sncRNA, specif
Using edgeR or DESeq2 to analyze allele-specific expression?
Answer: DESeq2 design for haplotype MPRA
Answer: Failure to download resources (MeSHDb) from AnnotationHub
Answer: Failure to download resources (MeSHDb) from AnnotationHub
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