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Voom
•
reset
1
vote
2
replies
306
views
Difference between logFC in limma-voom and weighted limma-voom
limma
voom
weighting
11 weeks ago
richardallenfriedmanbrooklyn
▴ 30
1
vote
2
replies
2.4k
views
Non-monotonic voomByGroup plot in Xenium data
XeniumIO
voom
3 months ago
richardallenfriedmanbrooklyn
▴ 30
4
votes
8
replies
1.8k
views
Should Xenium pseudobulk experiments be analysed like microarrays (limma) or like RNASeq (edgeR,Deseq2, or limma-voom)
Deseq2
edgeR
limma
xenium
Voom
3 months ago
richardallenfriedmanbrooklyn
▴ 30
1
vote
2
replies
3.7k
views
Is voomByGroup available in limma?
limmaGUI
voom
4 months ago
richardallenfriedmanbrooklyn
▴ 30
6
votes
5
replies
1.0k
views
How do I fix an odd voom plot in a combined dataset?
limma
Normalization
voom
updated 9 months ago by
James W. MacDonald
68k • written 10 months ago by
1sunmic2
• 0
0
votes
2
replies
866
views
sva: get a sv for RIN scores
limma
sva
variancePartition
voom
RNASeq
updated 10 months ago by
Gordon Smyth
53k • written 10 months ago by
Lina
▴ 10
1
vote
8
replies
2.4k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 12 months ago by
Adam Marstrand
• 0
2
votes
4
replies
1.1k
views
how limma work with continous variables
GordonSmyth
limmaGUI
lmFit
voom
updated 15 months ago by
Gordon Smyth
53k • written 15 months ago by
adR
▴ 40
4
votes
4
replies
1.7k
views
Using both voom and removeBatchEffect, will they double adjust the data or is it the right way to perform this
limma
adjustment
voom
removeBatchEffect
covariates
16 months ago
sukeshinik5
▴ 20
1
vote
2
replies
1.0k
views
Multi-factorial, longitudinal disease progression analysis with unbalanced patient sampling - duplicateCorrelation or blocking?
rna-seq
duplicateCorrelation
limma
voom
18 months ago • updated 17 months ago
Dylan.Sheerin
• 0
0
votes
2
replies
766
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 18 months ago by
taur.vil
• 0
0
votes
2
replies
846
views
voom(calcNormFactors()) for normalization of RNAseq counts for WGCNA
Voom
Limma
WGCNA
19 months ago
Gimena
• 0
5
votes
2
replies
1.5k
views
Filter genes by expression for voomLmFit
DEG
edgeR
limma
voom
filterByExpr
updated 23 months ago by
Gordon Smyth
53k • written 23 months ago by
annikagable
• 0
12
votes
5
replies
5.0k
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR
limma
voom
RNASeq
muscat
updated 5 months ago by
Geoff
• 0 • written 23 months ago by
annikagable
• 0
3
votes
4
replies
1.4k
views
Is it appropriate to apply linear mixed models to Voom transformed data?
stats
r
RNASeqData
limma
voom
23 months ago
pl23
• 0
2
votes
4
replies
1.4k
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom
RNASeq
limma
23 months ago
Tyler Sagendorf
▴ 10
5
votes
3
replies
1.2k
views
limma: More differentially expressed genes when factor included in model than when used as blocking factor
voom
blocking
limma
updated 2.1 years ago by
Gordon Smyth
53k • written 2.1 years ago by
Gerhard Thallinger
▴ 180
1
vote
6
replies
3.7k
views
Can I use EdgeR log2(cpm) instead of voom log2(cpm) to be analyzed as microarray data?
limma
edger
timecourse
without replicate
voom
written 9.0 years ago by
Camila Orellana
▴ 10
1
vote
1
reply
2.1k
views
How to do GSEA over limma + voom DGE ?
GSEA
voom
limma
updated 2.3 years ago by
Gordon Smyth
53k • written 2.3 years ago by
manuelsokolov
• 0
1
vote
5
replies
1.9k
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI
normalization
limma
voom
Normalization
updated 2.3 years ago by
Gordon Smyth
53k • written 2.3 years ago by
manuelsokolov
• 0
0
votes
1
reply
869
views
How to do PFS after Voom + Limma DGE ?
RNASeqData
limma
voom
PFS
DGE
updated 2.4 years ago by
James W. MacDonald
68k • written 2.4 years ago by
manuelsokolov
• 0
0
votes
1
reply
1.0k
views
Decidetest results summary different from results written as .csv file
edgeR
voom
decidetests
DEG
updated 2.6 years ago by
Gordon Smyth
53k • written 2.6 years ago by
Ankita
• 0
3
votes
4
replies
2.2k
views
Processing data with voom "expression values" and "weights"
limma
voom
updated 2.8 years ago by
Gordon Smyth
53k • written 2.8 years ago by
Edmond
• 0
1
vote
1
reply
992
views
Alternatives to Voom in experiments without replicate
limma
edgeR
Voom
updated 2.8 years ago by
Gordon Smyth
53k • written 2.8 years ago by
Mohammad
• 0
1
vote
3
replies
1.8k
views
diagnosing shifted MD plots/biased DEGs despite filtering
edgeR
voom
normalization
DifferentialExpression
updated 2.9 years ago by
Gordon Smyth
53k • written 2.9 years ago by
wiscoyogi
• 0
1
vote
1
reply
1.3k
views
Voom variance values: Some minor confusion about which variance is fed to lowess when using a design matrix of covariates
voom
updated 2.9 years ago by
Gordon Smyth
53k • written 2.9 years ago by
Brian
• 0
1
vote
10
replies
3.9k
views
multiple voom runs with duplicateCorrelation and negative values in limma
limma
voom
3.2 years ago
Hilary
▴ 40
2
votes
2
replies
2.0k
views
How to judge DEG analysis of these samples could be done by using limma::voom
limma
Clustering
voom
RNA-Seq
3.3 years ago
Yang Shi
▴ 10
2
votes
4
replies
2.1k
views
pre-ranked GSEA or not?
log2FC
DESeq2
GSEA
voom
limma
updated 3.3 years ago by
Gordon Smyth
53k • written 3.3 years ago by
Yang Shi
▴ 10
5
votes
6
replies
2.7k
views
Removing the duplicated genes before limma::voom by using limma::avereps
RSEM
avereps
voom
limma
RNASeq
3.3 years ago
Yang Shi
▴ 10
2
votes
4
replies
2.7k
views
Doing normalization when using voom for DEG analysis?
limma
RSEM
voom
Normalization
3.3 years ago
Yang Shi
▴ 10
2
votes
9
replies
4.0k
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA
r
RNASeqData
voom
limma
3.4 years ago
Peipei
• 0
2
votes
2
replies
2.6k
views
Handling NA values in limma's voom design matrix
limma
voom
design
NAs
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
dr
▴ 10
2
votes
4
replies
5.1k
views
Limma: How to deal with multiple batch effects and what is going on under the hood
limma
voom
updated 3.5 years ago by
Gordon Smyth
53k • written 3.5 years ago by
jmannhei
▴ 20
0
votes
3
replies
2.1k
views
limma voom contrasts
contrasts
limma
voom
updated 3.7 years ago by
Gordon Smyth
53k • written 3.7 years ago by
S T
▴ 90
0
votes
3
replies
1.5k
views
Need help with a contrast design for limma with a missing group?
limma
VOOM
updated 3.7 years ago by
Gordon Smyth
53k • written 3.7 years ago by
Ahdee
▴ 60
3
votes
5
replies
3.6k
views
limma-trend and voom results do not agree
limma
limma-trend
voom
RNASeq
4.0 years ago • updated 3.9 years ago
Tyler Sagendorf
▴ 10
3
votes
2
replies
2.3k
views
Multi-level design RRBS analysis with voomLmFit
edgeR
voomLmFit
limma
duplicateCorrelation
voom
updated 12 months ago by
Gordon Smyth
53k • written 3.9 years ago by
altintas.ali
• 0
0
votes
2
replies
3.2k
views
Mean-variance trend and identifying significant DE with large amount of biological change in expression in time-series data
voom
4.0 years ago • updated 3.9 years ago
hmviit
• 0
0
votes
2
replies
1.9k
views
voom flips the sign relative to log(cpm)
limma
limma-voom
rnaseq
voom
updated 4.1 years ago by
Gordon Smyth
53k • written 4.1 years ago by
taur.vil
• 0
0
votes
1
reply
3.1k
views
limma/voom for protein intensity data
limma
proteomics
voom
updated 4.1 years ago by
ATpoint
★ 5.0k • written 4.1 years ago by
Endre Sebestyén
▴ 70
0
votes
2
replies
1.4k
views
limma voom; fitted lines, factors that aren't of interest, and hypothesis testing
topTable
voom
contrasts
4.2 years ago
James
• 0
0
votes
2
replies
1.4k
views
voom-limma workflow for RNAseq samples with very different numbers of counts
voom
limma
RNASeq
4.6 years ago
annadv
• 0
0
votes
2
replies
7.9k
views
TPM data in limma/voom
voom
limma
DifferentialExpression
edgeR
4.7 years ago
RJM
• 0
1
vote
1
reply
2.0k
views
Limma-Voom - Time series experiment with one group (duplicateCorrelation() function)
voom
duplicateCorrelation()
timeseries
limma
updated 4.8 years ago by
Gordon Smyth
53k • written 4.9 years ago by
heikki.sarin
▴ 10
6
votes
13
replies
7.8k
views
How to use RSEM value to find differentially expressed genes
RSEM
limma
edgeR
voom
normalization
updated 3.3 years ago by
Gordon Smyth
53k • written 5.2 years ago by
harelarik
▴ 60
0
votes
5
replies
2.0k
views
isoform data, deep sequencing and voom
isoform
deep sequencing
voom
updated 5.2 years ago by
Gordon Smyth
53k • written 5.2 years ago by
Ina Hoeschele
▴ 620
0
votes
4
replies
1.7k
views
mean-variance trend plots with isoform and gene-level data from tximport
tximport
isoform
voom
updated 5.3 years ago by
Gordon Smyth
53k • written 5.3 years ago by
Ina Hoeschele
▴ 620
1
vote
4
replies
2.4k
views
How to compare a single RNA-Seq sample with microarray samples?
voom
rna-seq
microarray
updated 5.4 years ago by
James W. MacDonald
68k • written 5.4 years ago by
Sam
▴ 10
0
votes
1
reply
1.2k
views
Coefficients not estimable voom Lima with multiple numeric covariate
r
limma
design
rnaseq
voom
updated 5.4 years ago by
Gordon Smyth
53k • written 5.4 years ago by
Will
• 0
166 results • Page
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Replies
Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
I can reproduce this and have opened an issue on the code base https://github.com/Bioconductor/support.bioconductor.org/issues/112
Comment: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
we can investigate this issue. thank you for bringing it to our attention
Comment: deseq2 filter the low counts
by
Michael Love
43k
> But within each comparison (contrast), if I trace some DEGs back to the original salmon merged gene counts (or even to normalised counts)…
Comment: deseq2 filter the low counts
by
empty_mt
• 0
Thank you for you suggestion to look at the pre-filtering section in the DESeq2 vignettes - am I missing something? I have 6 treatments, …
Comment: deseq2 filter the low counts
by
Michael Love
43k
Have you seen this part: https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#pre-filtering
Votes
Bioconductor 3.22 Released!
Comment: "Watched Tags" are being erased rather than updated
Comment: deseq2 filter the low counts
Answer: DESeq2 interactions
Answer: Important: Bioconductor Release 3.22 Delayed
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