Showing : Voom • reset
1
vote
2
replies
350
views
Difference between logFC in limma-voom and weighted limma-voom
limma voom weighting
3 months ago richardallenfriedmanbrooklyn ▴ 30
1
vote
2
replies
2.5k
views
Non-monotonic voomByGroup plot in Xenium data
XeniumIO voom
4 months ago richardallenfriedmanbrooklyn ▴ 30
4
votes
8
replies
1.9k
views
Should Xenium pseudobulk experiments be analysed like microarrays (limma) or like RNASeq (edgeR,Deseq2, or limma-voom)
Deseq2 edgeR limma xenium Voom
4 months ago richardallenfriedmanbrooklyn ▴ 30
1
vote
2
replies
3.7k
views
Is voomByGroup available in limma?
limmaGUI voom
5 months ago richardallenfriedmanbrooklyn ▴ 30
6
votes
5
replies
1.1k
views
How do I fix an odd voom plot in a combined dataset?
limma Normalization voom
updated 10 months ago by 68k • written 10 months ago by • 0
0
votes
2
replies
904
views
sva: get a sv for RIN scores
limma sva variancePartition voom RNASeq
updated 11 months ago by 53k • written 11 months ago by ▴ 10
1
vote
8
replies
2.5k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma voom RNASeq edgeR Microarray
written 12 months ago by • 0
2
votes
4
replies
1.2k
views
how limma work with continous variables
GordonSmyth limmaGUI lmFit voom
updated 15 months ago by 53k • written 15 months ago by ▴ 40
4
votes
4
replies
1.7k
views
Using both voom and removeBatchEffect, will they double adjust the data or is it the right way to perform this
limma adjustment voom removeBatchEffect covariates
17 months ago sukeshinik5 ▴ 20
1
vote
2
replies
1.1k
views
Multi-factorial, longitudinal disease progression analysis with unbalanced patient sampling - duplicateCorrelation or blocking?
rna-seq duplicateCorrelation limma voom
18 months ago Dylan.Sheerin • 0
0
votes
2
replies
787
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma RNASeq voom
written 19 months ago by • 0
0
votes
2
replies
869
views
voom(calcNormFactors()) for normalization of RNAseq counts for WGCNA
Voom Limma WGCNA
19 months ago Gimena • 0
5
votes
2
replies
1.5k
views
Filter genes by expression for voomLmFit
DEG edgeR limma voom filterByExpr
updated 23 months ago by 53k • written 23 months ago by • 0
12
votes
5
replies
5.0k
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR limma voom RNASeq muscat
updated 5 months ago by • 0 • written 24 months ago by • 0
3
votes
4
replies
1.4k
views
Is it appropriate to apply linear mixed models to Voom transformed data?
stats r RNASeqData limma voom
2.0 years ago • updated 24 months ago pl23 • 0
2
votes
4
replies
1.4k
views
limma + voom combine voomWithQualityWeights and duplicateCorrelation
voom RNASeq limma
2.0 years ago Tyler Sagendorf ▴ 10
5
votes
3
replies
1.2k
views
limma: More differentially expressed genes when factor included in model than when used as blocking factor
voom blocking limma
updated 2.1 years ago by 53k • written 2.1 years ago by ▴ 180
1
vote
6
replies
3.7k
views
Can I use EdgeR log2(cpm) instead of voom log2(cpm) to be analyzed as microarray data?
limma edger timecourse without replicate voom
written 9.0 years ago by ▴ 10
1
vote
1
reply
2.1k
views
How to do GSEA over limma + voom DGE ?
GSEA voom limma
updated 2.4 years ago by 53k • written 2.4 years ago by • 0
1
vote
5
replies
2.0k
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI normalization limma voom Normalization
updated 2.4 years ago by 53k • written 2.4 years ago by • 0
0
votes
1
reply
888
views
How to do PFS after Voom + Limma DGE ?
RNASeqData limma voom PFS DGE
updated 2.4 years ago by 68k • written 2.4 years ago by • 0
0
votes
1
reply
1.1k
views
Decidetest results summary different from results written as .csv file
edgeR voom decidetests DEG
updated 2.6 years ago by 53k • written 2.6 years ago by • 0
3
votes
4
replies
2.2k
views
Processing data with voom "expression values" and "weights"
limma voom
updated 2.8 years ago by 53k • written 2.8 years ago by • 0
1
vote
1
reply
1.0k
views
Alternatives to Voom in experiments without replicate
limma edgeR Voom
updated 2.9 years ago by 53k • written 2.9 years ago by • 0
1
vote
3
replies
1.8k
views
diagnosing shifted MD plots/biased DEGs despite filtering
edgeR voom normalization DifferentialExpression
updated 3.0 years ago by 53k • written 3.0 years ago by • 0
1
vote
1
reply
1.4k
views
Voom variance values: Some minor confusion about which variance is fed to lowess when using a design matrix of covariates
voom
updated 3.0 years ago by 53k • written 3.0 years ago by • 0
1
vote
10
replies
3.9k
views
multiple voom runs with duplicateCorrelation and negative values in limma
limma voom
3.2 years ago Hilary ▴ 40
2
votes
2
replies
2.0k
views
How to judge DEG analysis of these samples could be done by using limma::voom
limma Clustering voom RNA-Seq
3.3 years ago Yang Shi ▴ 10
2
votes
4
replies
2.1k
views
pre-ranked GSEA or not?
log2FC DESeq2 GSEA voom limma
updated 3.3 years ago by 53k • written 3.3 years ago by ▴ 10
5
votes
6
replies
2.7k
views
Removing the duplicated genes before limma::voom by using limma::avereps
RSEM avereps voom limma RNASeq
3.3 years ago Yang Shi ▴ 10
2
votes
4
replies
2.7k
views
Doing normalization when using voom for DEG analysis?
limma RSEM voom Normalization
3.3 years ago Yang Shi ▴ 10
2
votes
9
replies
4.0k
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA r RNASeqData voom limma
3.4 years ago Peipei • 0
2
votes
2
replies
2.7k
views
Handling NA values in limma's voom design matrix
limma voom design NAs
updated 3.5 years ago by 53k • written 3.5 years ago by ▴ 10
2
votes
4
replies
5.1k
views
Limma: How to deal with multiple batch effects and what is going on under the hood
limma voom
updated 3.6 years ago by 53k • written 3.6 years ago by ▴ 20
0
votes
3
replies
2.2k
views
limma voom contrasts
contrasts limma voom
updated 3.7 years ago by 53k • written 3.7 years ago by ▴ 90
0
votes
3
replies
1.5k
views
Need help with a contrast design for limma with a missing group?
limma VOOM
updated 3.7 years ago by 53k • written 3.7 years ago by ▴ 60
3
votes
5
replies
3.7k
views
limma-trend and voom results do not agree
limma limma-trend voom RNASeq
4.0 years ago Tyler Sagendorf ▴ 10
3
votes
2
replies
2.3k
views
Multi-level design RRBS analysis with voomLmFit
edgeR voomLmFit limma duplicateCorrelation voom
updated 13 months ago by 53k • written 4.0 years ago by • 0
0
votes
2
replies
3.3k
views
Mean-variance trend and identifying significant DE with large amount of biological change in expression in time-series data
voom
4.0 years ago • updated 3.9 years ago hmviit • 0
0
votes
2
replies
2.0k
views
voom flips the sign relative to log(cpm)
limma limma-voom rnaseq voom
updated 4.2 years ago by 53k • written 4.2 years ago by • 0
0
votes
1
reply
3.1k
views
limma/voom for protein intensity data
limma proteomics voom
updated 4.2 years ago by ★ 5.0k • written 4.2 years ago by ▴ 70
0
votes
2
replies
1.4k
views
limma voom; fitted lines, factors that aren't of interest, and hypothesis testing
topTable voom contrasts
4.2 years ago James • 0
0
votes
2
replies
1.4k
views
voom-limma workflow for RNAseq samples with very different numbers of counts
voom limma RNASeq
4.7 years ago • updated 4.6 years ago annadv • 0
0
votes
2
replies
8.0k
views
TPM data in limma/voom
voom limma DifferentialExpression edgeR
4.7 years ago RJM • 0
1
vote
1
reply
2.0k
views
Limma-Voom - Time series experiment with one group (duplicateCorrelation() function)
voom duplicateCorrelation() timeseries limma
updated 4.9 years ago by 53k • written 4.9 years ago by ▴ 10
6
votes
13
replies
7.9k
views
How to use RSEM value to find differentially expressed genes
RSEM limma edgeR voom normalization
updated 3.3 years ago by 53k • written 5.2 years ago by ▴ 60
0
votes
5
replies
2.0k
views
isoform data, deep sequencing and voom
isoform deep sequencing voom
updated 5.3 years ago by 53k • written 5.3 years ago by ▴ 620
0
votes
4
replies
1.7k
views
mean-variance trend plots with isoform and gene-level data from tximport
tximport isoform voom
updated 5.4 years ago by 53k • written 5.4 years ago by ▴ 620
1
vote
4
replies
2.4k
views
How to compare a single RNA-Seq sample with microarray samples?
voom rna-seq microarray
updated 5.4 years ago by 68k • written 5.4 years ago by ▴ 10
0
votes
1
reply
1.3k
views
Coefficients not estimable voom Lima with multiple numeric covariate
r limma design rnaseq voom
updated 5.4 years ago by 53k • written 5.4 years ago by • 0
166 results • Page 1 of 4
Recent ...
Replies
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication? by Ahmed Salah • 0
Thank you very much, @ATpoint and @kevin , for your valuable insights. I have carefully checked all your suggestions. First, I examined th…
Answer: Finding duplicated entries in GRangesList by Kevin Blighe ★ 4.0k
The duplicated and unique functions operate element-wise on GRangesList objects. This means that they apply the respective function to each…
Answer: DESeq2 on non-RNASeq counts data by Kevin Blighe ★ 4.0k
The use of DESeq2 for count data from artificial sequences is acceptable, as the program models counts via a negative binomial distribution…
Answer: After running samtools, the featureCounts command crashed. by Kevin Blighe ★ 4.0k
Hello Rui Tang, The buffer overflow error in featureCounts that you encountered is likely due to a compatibility issue between the Subre…
Answer: Problem with the TMB plot by Kevin Blighe ★ 4.0k
The issue that you describe with the tumor mutational burden (TMB) plots appearing linear and lacking consistent TMB values across samples …
Votes
Answer: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
Answer: Post translational modifications and phosphoproteomics in limpa?
Answer: Post translational modifications and phosphoproteomics in limpa?
Answer: Identifying differentially expressed genes in individual samples from a large co
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