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VOOM
•
reset
2
votes
9
replies
234
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA
r
RNASeqData
voom
limma
9 days ago • updated 4 days ago
Peipei
• 0
2
votes
2
replies
139
views
Handling NA values in limma's voom design matrix
limma
voom
design
NAs
updated 6 weeks ago by
Gordon Smyth
45k • written 6 weeks ago by
dr
▴ 10
1
vote
4
replies
288
views
Limma: How to deal with multiple batch effects and what is going on under the hood
limma
voom
updated 8 weeks ago by
Gordon Smyth
45k • written 9 weeks ago by
jmannhei
▴ 10
0
votes
3
replies
230
views
limma voom contrasts
contrasts
limma
voom
updated 3 months ago by
Gordon Smyth
45k • written 3 months ago by
S T
▴ 80
0
votes
3
replies
290
views
Need help with a contrast design for limma with a missing group?
limma
VOOM
updated 4 months ago by
Gordon Smyth
45k • written 4 months ago by
Ahdee
▴ 50
2
votes
5
replies
581
views
limma-trend and voom results do not agree
limma
limma-trend
voom
RNASeq
7 months ago • updated 6 months ago
Tyler Sagendorf
• 0
3
votes
2
replies
380
views
Multi-level design RRBS analysis with voomLmFit
edgeR
voomLmFit
limma
duplicateCorrelation
voom
7 months ago
altintas.ali
• 0
0
votes
2
replies
421
views
Mean-variance trend and identifying significant DE with large amount of biological change in expression in time-series data
voom
7 months ago • updated 6 months ago
hmviit
• 0
0
votes
2
replies
394
views
voom flips the sign relative to log(cpm)
limma
limma-voom
rnaseq
voom
updated 9 months ago by
Gordon Smyth
45k • written 9 months ago by
taur.vil
• 0
0
votes
1
reply
384
views
limma/voom for protein intensity data
limma
proteomics
voom
updated 9 months ago by
ATpoint
★ 1.3k • written 9 months ago by
Endre Sebestyén
▴ 60
0
votes
2
replies
353
views
limma voom; fitted lines, factors that aren't of interest, and hypothesis testing
topTable
voom
contrasts
10 months ago
James
• 0
0
votes
2
replies
366
views
voom-limma workflow for RNAseq samples with very different numbers of counts
voom
limma
RNASeq
15 months ago
annadv
• 0
0
votes
2
replies
1.1k
views
TPM data in limma/voom
voom
limma
DifferentialExpression
edgeR
16 months ago
RJM
• 0
1
vote
1
reply
663
views
Limma-Voom - Time series experiment with one group (duplicateCorrelation() function)
voom
duplicateCorrelation()
timeseries
limma
updated 18 months ago by
Gordon Smyth
45k • written 18 months ago by
heikki.sarin
▴ 10
6
votes
13
replies
1.7k
views
How to use RSEM value to find differentially expressed genes
RSEM
limma
edgeR
voom
normalization
updated 22 months ago by
Gordon Smyth
45k • written 22 months ago by
harelarik
▴ 50
0
votes
5
replies
520
views
isoform data, deep sequencing and voom
isoform
deep sequencing
voom
updated 22 months ago by
Gordon Smyth
45k • written 22 months ago by
Ina Hoeschele
▴ 620
0
votes
4
replies
391
views
mean-variance trend plots with isoform and gene-level data from tximport
tximport
isoform
voom
updated 23 months ago by
Gordon Smyth
45k • written 23 months ago by
Ina Hoeschele
▴ 620
1
vote
4
replies
376
views
How to compare a single RNA-Seq sample with microarray samples?
voom
rna-seq
microarray
updated 2.0 years ago by
James W. MacDonald
60k • written 2.0 years ago by
Sam
▴ 10
0
votes
1
reply
302
views
Coefficients not estimable voom Lima with multiple numeric covariate
r
limma
design
rnaseq
voom
updated 2.0 years ago by
Gordon Smyth
45k • written 2.0 years ago by
Will
• 0
2
votes
6
replies
617
views
linear mixed models using edgeR+limma
linear mixed model
edger
limma
voom
RNA-seq
updated 2.1 years ago by
Gordon Smyth
45k • written 2.1 years ago by
weichengz
• 0
1
vote
5
replies
482
views
Limma for proteomic data! Please Help!
limma
voom
lmfit
ebayesfit
updated 2.2 years ago by
Gordon Smyth
45k • written 2.2 years ago by
sa825
• 0
0
votes
2
replies
224
views
estimating variability in absence of replicates for edgeR
edgeR
RNAseq
voom
variability estimator
updated 2.3 years ago by
Gordon Smyth
45k • written 2.3 years ago by
hcnbox
• 0
2
votes
6
replies
475
views
paired and unpaired test at the same time with voom limma
limma
voom
rna-seq
paired test
unpaired test
updated 2.3 years ago by
Gordon Smyth
45k • written 2.3 years ago by
dagsbio
• 0
6
votes
5
replies
778
views
How to know if I should use voomWithQualityWeights() or not?
limma
RNA-Seq
voom
voomWithQualityWeights
edgeR
updated 2.3 years ago by
Gordon Smyth
45k • written 2.3 years ago by
pedrodcb
• 0
0
votes
0
replies
282
views
RNAseq/miRNAseq normalization for calculating gene correlations, survival analysis, and exploratory graphing
limma
edger
voom
rnaseq
mirnaseq
2.4 years ago
atakanekiz
▴ 30
4
votes
7
replies
675
views
Can I use (length bias normalization) from packages like EDAseq or cqn for input into VOOM?
limma
voom
edaseq
edger
rnaseq
updated 2.5 years ago by
Gordon Smyth
45k • written 2.5 years ago by
Ahdee
▴ 50
3
votes
6
replies
1.3k
views
Understanding voom behavior when model matrix is used with/without intercept for a continuous variable
limma
voom
rnaseq
model.matrix
intercept
updated 2.6 years ago by
Gordon Smyth
45k • written 2.6 years ago by
atakanekiz
▴ 30
0
votes
0
replies
342
views
limma-voom group design and its effect on sva
limma
voom
group-design
sva
2.6 years ago
kentfung
• 0
2
votes
4
replies
628
views
Limma gives big p-values for large LFC
limma
voom
updated 2.7 years ago by
Gordon Smyth
45k • written 2.7 years ago by
chipolino
• 0
4
votes
5
replies
507
views
Defining the design for a complicated multi-level experiment in Limma
limma
RNASeq
R
voom
updated 2.7 years ago by
James W. MacDonald
60k • written 2.7 years ago by
Colari19
▴ 20
0
votes
1
reply
614
views
Variance unstable after fitting to linear model with limma-voom pipeline
limma
voom
2.7 years ago
kentfung
• 0
4
votes
3
replies
849
views
Blocking factor in limma/voom vs. variancePartition/dream analysis.
limma
voom
variancePartition
dream
updated 2.7 years ago by
gabriel.hoffman
▴ 90 • written 3.0 years ago by
Ben
▴ 50
0
votes
3
replies
372
views
Design matrix for cohort with samples outside "block"
limma
voom
sva
2.9 years ago
kentfung
• 0
0
votes
3
replies
426
views
How to apply a linear mixed model onto an RNA-Seq data?
limma
linear mixed model
voom
rna-seq
2.9 years ago
Assa Yeroslaviz
★ 1.5k
4
votes
4
replies
495
views
normalization of RNA-seq data after over-expressing the transcription factors
limma
deseq2
voom
edgeR
updated 2.9 years ago by
Aaron Lun
★ 27k • written 2.9 years ago by
Bogdan
▴ 640
3
votes
4
replies
1.1k
views
MDS plotting within the limma/voom workflow
limma
voom
deseq2
updated 3.0 years ago by
Gordon Smyth
45k • written 3.0 years ago by
Ben
▴ 50
1
vote
5
replies
577
views
Time course experiment using limma with voom
limma
voom
RNA Seq
Time-course experiments
updated 3.0 years ago by
Aaron Lun
★ 27k • written 3.0 years ago by
mahes.muniandy
• 0
2
votes
2
replies
492
views
voomWithQualityWeights together with covariate in design matrix
limma
voom
RNA-seq
voomWithQualityWeights
updated 3.1 years ago by
Matthew Ritchie
▴ 1000 • written 3.1 years ago by
Julien Roux
▴ 90
1
vote
2
replies
499
views
Why does limma voom E value give different result than the voom object
limma
voom
rna-seq
updated 3.1 years ago by
thokall
▴ 160 • written 3.1 years ago by
Ahdee
▴ 50
5
votes
7
replies
1.1k
views
limma - blocking or regressing out nuisance variable
limma
voom
RNA-seq
updated 3.2 years ago by
James W. MacDonald
60k • written 3.2 years ago by
biomiha
▴ 20
0
votes
3
replies
650
views
~50% significant genes from RNA (NanoString) Differential Expression Analysis
limma
microarray
voom
3.3 years ago
rueben.bautista7
• 0
7
votes
3
replies
2.0k
views
VOOM vs VSN vs log2 to transform proteome data before LIMMA analysis
r
limma
voom
vsn
updated 3.3 years ago by
Wolfgang Huber
13k • written 3.3 years ago by
Sebastian Hesse
▴ 70
12
votes
4
replies
3.3k
views
differential expression analysis of htseq data with edgeR/voom
voom
htseq
edgeR
limma
RNASeq123
updated 3.5 years ago by
Gordon Smyth
45k • written 5.1 years ago by
wd
▴ 30
2
votes
2
replies
595
views
Different results from limma depending on whether or not I have an intercept term in my model
limma
lmfit
voom
limma design matrix
voomwithqualityweights
updated 3.5 years ago by
Gordon Smyth
45k • written 3.5 years ago by
kediggins
▴ 10
5
votes
5
replies
879
views
t-statistic from various limma-approaches does not follow the theoretical distribution
limma
t-statistic
voom
voomwithqualityweights
robust regression
3.6 years ago
brynedal
▴ 30
3
votes
6
replies
1.2k
views
Controlling numerical covariates: differential gene expression with limma voom
limma
voom
differential gene expression
rnaseq
R
updated 3.6 years ago by
mikhael.manurung
▴ 260 • written 3.6 years ago by
Onyi Ukay
• 0
2
votes
4
replies
789
views
Inconsistent result between limma/voom and DESeq2
deseq2
limma
voom
limma-voom
rnaseq
updated 3.6 years ago by
Gordon Smyth
45k • written 3.6 years ago by
bharata1803
▴ 40
5
votes
4
replies
786
views
Limma Voom duplicateCorrelation Design
limma
voom
duplicatecorrelation
updated 3.6 years ago by
Aaron Lun
★ 27k • written 3.6 years ago by
bruce.moran
▴ 30
2
votes
2
replies
718
views
voom variance modeling: lowess curve fitting notation misunderstanding?
voom
updated 3.6 years ago by
Gordon Smyth
45k • written 3.6 years ago by
njbernstein
• 0
8
votes
8
replies
3.7k
views
Voom transformation from counts and normalization to negative values
rnaseq
voom
limma
tcga
statistics
updated 3.8 years ago by
Ryan C. Thompson
7.8k • written 3.8 years ago by
Beginner
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Comment: Using Age or other numerical variable to model expression in deseq2
by
krushnach80
• 0
"Why is that" no as an example i was citing that.."certainly don't want to make the interaction between the nuisance variable and your vari…
Comment: Using Age or other numerical variable to model expression in deseq2
by
James W. MacDonald
60k
You have to use age from that post? Why is that? It is rarely optimal to categorize age rather than use as a continuous variable. It takes …
Comment: Using Age or other numerical variable to model expression in deseq2
by
krushnach80
• 0
if I have to use age from this [post][1] i think this is more cleaner way to make group or make category of age groups [1]: https://su…
Comment: Using Age or other numerical variable to model expression in deseq2
by
krushnach80
• 0
I know the result but i was not clear about the interpretation because if i use that numerical variable which is turned into factor for exa…
Comment: I got error message about DESeqDataSetFromMatrix
by
swbarnes2
★ 1.1k
Well, what do you think the error means based on what it says?
Votes
Comment: Using Age or other numerical variable to model expression in deseq2
Comment: Using Age or other numerical variable to model expression in deseq2
Answer: Using Age or other numerical variable to model expression in deseq2
Answer: Using Age or other numerical variable to model expression in deseq2
Answer: Trouble importing my own Gene Ontology Annotation into topGO
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