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2
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150
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competitive enrichment test yields too few significant results, self-contained too many
limma
DESeq2
fry
edgeR
10 hours ago • updated 3 hours ago
bbao
▴ 10
0
votes
1
reply
1.1k
views
How to avoid losing a sample as reference running an individual differential expression analysis (LIMMA)
Microarray
limma
BRAIN
Microarray
limma
BRAIN
updated 21 hours ago by
Gordon Smyth
52k • written 16.4 years ago by
Dmitriy Verkhoturov
▴ 10
0
votes
2
replies
296
views
Install old version of DESeq2
apeglm
Install
DESeq2
version
updated 14 hours ago by
James W. MacDonald
68k • written 1 day ago by
Asma
• 0
0
votes
1
reply
278
views
discrepancy in IDs from file and tx2gene (IDs don't match)
tximport
DESeq2
updated 1 day ago by
James W. MacDonald
68k • written 2 days ago by
IUH
• 0
1
vote
1
reply
1.3k
views
Best practice for handling large data (matrix with >2^31-1 non-zero elements) in SingleCellExperiment?
SingleCellExperiment
updated 2 days ago by
Aaron Lun
★ 28k • written 2 days ago by
Fabian
▴ 10
0
votes
0
replies
29
views
Boolean Gate Combination for Any 2 Positive Events in flowWorkspace
flowWorkspace
FlowCytometry
2 days ago
Valeria
• 0
0
votes
0
replies
25
views
News:
Announcing a New Journal - Bioinformatics Methods and Applications - Now Open for Submissions
Bioconductor
3 days ago
Wei Shi
★ 3.6k
1
vote
2
replies
1.5k
views
Regarding the issue of background genes in enrichment analysis
KEGG
goTools
seqTools
updated 2 days ago by
zalinkaelisa
• 0 • written 7 days ago by
17506483572
▴ 10
0
votes
3
replies
2.0k
views
Applying lmFit(), eBayes() and topTable() for modeling associations between NMR-derived metabolic measures and various traits
limma
Bioconductor
Metabolomics
updated 7 days ago by
Gordon Smyth
52k • written 10 days ago by
anni
• 0
2
votes
4
replies
2.9k
views
I would like to perform differential expression analysis of lncRNAs using DESeq2 and edgeR.
diffGeneAnalysis
lncRNA
10 days ago • updated 9 days ago
John
▴ 10
2
votes
1
reply
1.6k
views
ExperimentHub full metadata
ExperimentHub
updated 10 days ago by
shepherl
4.2k • written 11 days ago by
igor
▴ 50
0
votes
2
replies
760
views
gene detection across experiments
DESeq2
Normalization
12 days ago • updated 11 days ago
Mara
• 0
0
votes
3
replies
1.4k
views
RNA-seq: TPM batch effect correction
rnaseqDTU
Normalization
updated 14 days ago by
James W. MacDonald
68k • written 14 days ago by
Samuel
• 0
1
vote
3
replies
1.4k
views
How can I correctly use phyloseq with Docker?
phylo
phyloseq
Docker
written 10 weeks ago by
Franz Bachmann
• 0
5
votes
11
replies
2.7k
views
parameters of filterByExpr
edgeR
DESeq2
filterByExpr
written 10 weeks ago by
caroline.zanchi
▴ 10
1
vote
8
replies
5.0k
views
DiffBind - question on the use of filtered or unfiltered input data
DiffBind
updated 11 days ago by
Rivers
• 0 • written 2.2 years ago by
Oscar
▴ 10
0
votes
5
replies
1.8k
views
compute_fasta_digest does not match Salmon index hash
tximeta
written 3 months ago by
Alex
• 0
0
votes
5
replies
584
views
WGCNA
WGCNA
Normalization
RNASeq
written 4 months ago by
Vishnu
• 0
3
votes
4
replies
4.9k
views
Analysis of clusters of a heatmap with enrichGO of clusterProfiler: choice fo the categories to visualize
clusterProfiler
enrichGO
ComplexHeatmap
updated 15 days ago by
James W. MacDonald
68k • written 23 days ago by
caroline.zanchi
▴ 10
0
votes
3
replies
908
views
TopGO question
topGO
top
updated 16 days ago by
James W. MacDonald
68k • written 18 days ago by
Hannah
• 0
0
votes
2
replies
817
views
Starting with a clean bioconductor rstudio container
Bioconductor
updated 18 days ago by
ATpoint
★ 4.9k • written 22 days ago by
Ramiro
• 0
0
votes
1
reply
2.2k
views
ChAMP pipeline error (champ.import)
software error
updated 18 days ago by
macdonaldfrench7286566
• 0 • written 6.3 years ago by
mhebba
• 0
0
votes
2
replies
317
views
Problem in Chip-seq data analysis using MACS3
chipseq
MACSdata
updated 18 days ago by
ATpoint
★ 4.9k • written 21 days ago by
Gaurav
• 0
0
votes
4
replies
2.7k
views
Pre-filtering, adjusting independent filtering, or lfcthreshold() on DESeq2? Low counts and dropouts produce interesting volcano plots.
DESeq2
4 weeks ago • updated 17 days ago
Alexander
• 0
0
votes
1
reply
506
views
Which GTF file to use for creation of tx2gene files?
tximport
updated 21 days ago by
Michael Love
43k • written 22 days ago by
Nicholas
• 0
1
vote
1
reply
1.9k
views
DESeq2 LRT: Testing Effects of Treatment1, Treatment2, and Their Interaction in a Four-Factor Design
DESeq2
multifactorialdesign
interaction
InteractionSet
updated 21 days ago by
Michael Love
43k • written 22 days ago by
Integrative
• 0
0
votes
2
replies
990
views
6 groups to compare DESeq2 (Wald vs LRT)
DESeq2
updated 21 days ago by
Michael Love
43k • written 24 days ago by
Rocio
• 0
0
votes
3
replies
740
views
Many transcripts missing from tx2gene created from Ensembl GTF file
kallisto
tximport
ensembl
updated 14 days ago by
Abbott
• 0 • written 22 days ago by
Nicholas
• 0
1
vote
2
replies
3.5k
views
Is voomByGroup available in limma?
limmaGUI
voom
25 days ago
richardallenfriedmanbrooklyn
▴ 30
0
votes
4
replies
1.5k
views
How to use goana function with zebrafish genome from org.Dr.eg.db
org.Dr.eg.db
zebrafish
limma
25 days ago • updated 24 days ago
Eric
• 0
1
vote
3
replies
1.1k
views
News:
Ensembl 114 / Ensembl Genomes 61 has been released!
biomaRt
ensembldb
written 9 weeks ago by
Aleena_Ensembl
▴ 30
0
votes
0
replies
25
views
Spam:
wealthdt5
weaver
wealth
27 days ago
Enfurry
0
0
votes
0
replies
122
views
Y-axis break ihn Volcano Plot
EnhancedVolcano
Y-axis_break
Volcano_Plot
28 days ago
rajdeepboral00
• 0
0
votes
0
replies
102
views
getQC(RGset.raw) from IlluminaHumanMethylationMSAmanifest
getQC
hcg110.db
updated 29 days ago by
shepherl
4.2k • written 29 days ago by
zwang072005
• 0
3
votes
2
replies
1.2k
views
Biological replicates consideration in DESeq2 design formula.
DESeq2
RNASeqData
RNASeq
written 5 weeks ago by
Christian
▴ 20
1
vote
2
replies
2.4k
views
Deseq2 paired sample in the design is considered as "batch"?
DESeq2
written 5 weeks ago by
corranna98
• 0
0
votes
0
replies
112
views
Job:
Job: Ensembl Dataflow Coordinator
JobPosting
ensembldb
4 weeks ago
Aleena_Ensembl
▴ 30
0
votes
1
reply
388
views
Job:
Job: Ensembl Genebuild Project Leader
JobPosting
ensembldb
updated 11 days ago by
Kyleronsidine191
• 0 • written 4 weeks ago by
Aleena_Ensembl
▴ 30
0
votes
1
reply
1.2k
views
Retrieve Salmon transcript quantification file from recount3
recount3
updated 4 weeks ago by
Leonardo Collado Torres
★ 1.1k • written 5 weeks ago by
kyn
▴ 10
0
votes
1
reply
1.0k
views
error from library(IlluminaHumanMethylationMSAmanifest)
MSAarray25
hcg110.db
updated 4 weeks ago by
James W. MacDonald
68k • written 4 weeks ago by
zwang072005
• 0
0
votes
1
reply
779
views
Source / binary version mismatch for rGADEM
rGADEM
updated 4 weeks ago by
shepherl
4.2k • written 4 weeks ago by
Jay hesselberth
• 0
0
votes
9
replies
13k
views
Bioconductor version cannot be validated
BiocManager
Bioconductor
updated 4 weeks ago by
Hadi
• 0 • written 3.9 years ago by
charlotte
▴ 10
3
votes
1
reply
3.6k
views
DESeq2 empirical bayes: variance of the prior for log fold changes (LFC)
DESeq2
updated 4 weeks ago by
Michael Love
43k • written 5 weeks ago by
Chenghao
▴ 10
0
votes
2
replies
1.1k
views
Interaction in Design Experimental
rnaseqGene
5 weeks ago
Ferdinand David
• 0
0
votes
0
replies
139
views
Job:
Lab Head positions at the WEHI, Melbourne, Australia
JobPosting
Bioconductor
5 weeks ago
Gordon Smyth
52k
1
vote
3
replies
2.0k
views
Heatmap of the Count Matrix
rnaseqGene
updated 3 days ago by
gta5movilecom
• 0 • written 5 weeks ago by
Ferdinand David
• 0
0
votes
1
reply
369
views
DESeq2 results
result
DESeq2
subsetting
updated 5 weeks ago by
ATpoint
★ 4.9k • written 5 weeks ago by
rajdeepboral00
• 0
0
votes
0
replies
111
views
DiffBind plot.profile error
BiocParallel
DiffBind
5 weeks ago
this_is_me_trying
• 0
0
votes
2
replies
7.8k
views
Bioconductor version cannot be validated
Bioconductor
Annotation
Ann
updated 4 weeks ago by
Hadi
• 0 • written 22 months ago by
Aytaç
• 0
0
votes
4
replies
775
views
Position of the legend object in complexHeatmap
ComplexHeatmap
5 weeks ago
caroline.zanchi
▴ 10
46,560 results • Page
1 of 932
Recent ...
Replies
Comment: competitive enrichment test yields too few significant results, self-contained t
by
bbao
▴ 10
Hi Gordon, Thanks for the advice, I gave camera a try, and it yielded around ~1,000 statistically significant gene sets from the same 11,0…
Answer: competitive enrichment test yields too few significant results, self-contained t
by
Gordon Smyth
52k
Actually, preranked GSEA is known to be highly anti-conservative, because it ignores inter-gene correlations, and can give significant resu…
Comment: competitive enrichment test yields too few significant results, self-contained t
by
ATpoint
★ 4.9k
Lets see your code. 8000 significant genesets indicate a flaw somewhere.
Comment: Install old version of DESeq2
by
James W. MacDonald
68k
To add to what Lori asks, this builds for me, using Dockerhub on Windows: ``` C:\Users\jmacdon\Desktop>docker build . [+] Building 477.7s …
Comment: How to do simple Fisher' exact test for overlapped genomic interval in R?
by
Keepa
• 0
https://nowtext.app/
Votes
Comment: competitive enrichment test yields too few significant results, self-contained t
Answer: competitive enrichment test yields too few significant results, self-contained t
Regarding the issue of background genes in enrichment analysis
Best practice for handling large data (matrix with >2^31-1 non-zero elements) in SingleCellExperiment?
Comment: How can I avoid artifacts in gene set/pathway scoring by UCell and similar algor
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