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0
votes
0
replies
16
views
Error compiling Rbowtie2 in R-4.4 (Bioconductor 3.20 and probably 3.19)
Rbowtie
8 hours ago
ja.garcia
• 0
1
vote
2
replies
187
views
Detecting genomic signatures of drug resistance breast cancer in transcriptomic data
TransOmicsData
signatureSearchData
StatisticalMethod
written 8 weeks ago by
olusolaxx
• 0
0
votes
2
replies
46k
views
SingleR Label.Ont
SingleR
written 16 months ago by
saeed.khalili
• 0
0
votes
0
replies
20
views
Curiously shaped volcano plot with using oarfish + swish to do differential gene expression on long-read RNA-seq
fishpond
fish
11 hours ago
brown.annaleigh
• 0
0
votes
0
replies
16
views
cnvEQTL : Error in cpm.default(y, lib.size = lib.size) : NA counts not allowed
CNVRanger
16 hours ago
xiaofeiwang18266
▴ 50
0
votes
0
replies
22
views
SingleCellExperiment convenience function for metadata+expression?
SpatialExperiment
SingleCellExperiment
SpatialFeatureExperiment
21 hours ago
sarah.williams
• 0
2
votes
5
replies
353
views
Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
limma
edgeR
biomaRt
updated 1 day ago by
James W. MacDonald
67k • written 1 day ago by
Jonathan
▴ 10
0
votes
1
reply
75
views
XCMS Data Processing
r
xcms
metabolomi
NTA
Metabolomics
updated 1 day ago by
James W. MacDonald
67k • written 3 days ago by
raklein
• 0
0
votes
2
replies
105
views
Error using useMart or useEnsembl
biomaRt
updated 1 day ago by
James W. MacDonald
67k • written 1 day ago by
rayanelkholdi
• 0
2
votes
1
reply
94
views
Error in download.file for TCGAutils::symbolsToRanges
TCGAutils
updated 1 day ago by
James W. MacDonald
67k • written 1 day ago by
xiaofeiwang18266
▴ 50
0
votes
11
replies
376
views
Problems with oarfish + tximport + swish
fishpond
tximport
tximeta
updated 4 hours ago by
Michael Love
43k • written 2 days ago by
brown.annaleigh
• 0
0
votes
2
replies
194
views
DESeq2 Heatmap
heatmaps
updated 2 days ago by
swbarnes2
★ 1.4k • written 3 days ago by
pm_25
• 0
0
votes
4
replies
189
views
Biomart and ensembl not working?
ensembldb
biomaRt
updated 2 days ago by
James W. MacDonald
67k • written 2 days ago by
Zuzer
• 0
0
votes
1
reply
79
views
Unknown gene transcripts after annotatr
annotatr
updated 2 days ago by
James W. MacDonald
67k • written 2 days ago by
Zach
• 0
0
votes
1
reply
71
views
Improve performance of qusage aggregateGeneSet
qusage
updated 3 days ago by
James W. MacDonald
67k • written 3 days ago by
oliverd
• 0
0
votes
0
replies
53
views
gsameth GSA: probe annotation to multiple genes
missMethyl
gsameth
4 days ago • updated 3 days ago
nurkenber
• 0
0
votes
0
replies
41
views
Testing Differential Expression with Interaction Effect Between Categorical and Continuous Variable with Repeated (Paired) Measures using variancePar…
limma
variancePartition
4 days ago
GJ
• 0
0
votes
1
reply
68
views
promoters_txdb <- promoters(txdb, upstream = 2000) is not 2000
TxDb.Hsapiens.UCSC.hg38.knownGene
updated 4 days ago by
James W. MacDonald
67k • written 4 days ago by
linouhao
▴ 20
0
votes
0
replies
38
views
News:
Online course: Gene set enrichment analysis in R/Bioconductor
GSEABenchmarkeR
5 days ago
info
▴ 10
0
votes
1
reply
122
views
org.Hs.eg.db issue
org.Hs.eg.db
updated 4 days ago by
shepherl
4.1k • written 6 days ago by
k.panov
• 0
0
votes
2
replies
342
views
Paired analysis in proteomics data for DE while interested in only a particular subset of the data
limma
modelmatrix
pairedanalysis
Proteomics
written 7 days ago by
svlachavas
▴ 840
0
votes
2
replies
209
views
iam not able to do retrive data using GEOquery R package
HELP
updated 7 days ago by
James W. MacDonald
67k • written 7 days ago by
ramya20kumar20
• 0
2
votes
5
replies
275
views
Differential variability modelling with vst() expression
limma
DESeq2
written 8 days ago by
jtsy6383
• 0
0
votes
2
replies
116
views
Installation error for Rsamtools.
Rsamtools
updated 7 days ago by
ATpoint
★ 4.6k • written 8 days ago by
Sheila
• 0
2
votes
8
replies
488
views
Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) returning drastically different numbers of transcripts
AnnotationDbi
EnsDb.Mmusculus.v79
biomaRt
org.Mm.eg.db
updated 7 days ago by
James W. MacDonald
67k • written 8 days ago by
Nicholas
• 0
0
votes
2
replies
334
views
DESeq2 analysis with transcript counts
Transcriptomics
RNASeq
DESeq2
8 days ago • updated 6 days ago
sindhuri
• 0
0
votes
0
replies
47
views
Issue when attempting to use output from DECIPHER IDtaxa (pr2 V5) with dada2/phyloseq
DECIPHER
taxonomyassignment
phyloseq
dada2
Pr2V5
9 days ago
Maggie
• 0
2
votes
9
replies
691
views
Why did PCA plots look exactly the same after adjusting for batch covariate in DESeq2 design?
DESeq2
BatchEffect
updated 2 days ago by
Michael Love
43k • written 9 days ago by
Quang
▴ 10
0
votes
5
replies
215
views
Diffbind interpretation of data - fold change
DiffBind
10 days ago
Vera
• 0
1
vote
1
reply
265
views
Considerations behind the choice of RNAseq Differential Expression Analysis Tools
fishpond
DESeq2
rnaseqDTU
updated 10 days ago by
Michael Love
43k • written 10 days ago by
muhammadsohailraza
• 0
0
votes
1
reply
75
views
No preprocessing allowed for EPICv2 with DMRcate
missMethyl
DMRcate
updated 10 days ago by
James W. MacDonald
67k • written 10 days ago by
maximuscfyeung
• 0
1
vote
2
replies
293
views
Package `not available for Bioconductor version '3.20'` when it should be
consensusOV
genefu
10 days ago
Levi Waldron
★ 1.1k
0
votes
0
replies
44
views
Trouble with double delimiters in .fcs file (flowCore package)
flowCore
FlowCytometry
FlowCytometryData
11 days ago
vpochic
• 0
0
votes
4
replies
211
views
Newbie Question - adding metadata to an analysis
Biobase
11 days ago • updated 9 days ago
Bryan H
• 0
0
votes
1
reply
146
views
Want numbers in DESeq to be seen as numerical, not categorical
DESeq2
updated 11 days ago by
swbarnes2
★ 1.4k • written 11 days ago by
natalievillafranca
• 0
0
votes
2
replies
237
views
MissMethyl failed to use gometh function for EPICv2
missMethyl
EPICv2manifest
updated 11 days ago by
James W. MacDonald
67k • written 11 days ago by
maximuscfyeung
• 0
0
votes
1
reply
101
views
Correlation between gene clusters
genomes
RNASeqData
written 11 days ago by
Le
• 0
0
votes
2
replies
195
views
Mean variance curve troubleshoot filtering
limmavoom
filtering
edgeR
RNASeqData
meanvariancetrend
13 days ago • updated 11 days ago
gingergeiger22
• 0
0
votes
0
replies
64
views
Error ("could not find function "EM_estimate"") when running ENmix mpreprocess() on Microsoft Azure
Preprocessing
ENmix
13 days ago
balf0020
• 0
0
votes
1
reply
330
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 14 days ago by
Michael Love
43k • written 24 days ago by
Jimmy Mak
• 0
2
votes
1
reply
331
views
Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2
RNASeqData
updated 14 days ago by
Michael Love
43k • written 21 days ago by
KR
• 0
0
votes
1
reply
1.3k
views
How to increase font for gene name and increase arrow size for GVIZ's ( UcscTrack function ) track?
DMRcate
Gviz
updated 15 days ago by
ming
• 0 • written 3.1 years ago by
Ahdee
▴ 50
6
votes
5
replies
518
views
How do I fix an odd voom plot in a combined dataset?
limma
Normalization
voom
updated 10 days ago by
James W. MacDonald
67k • written 16 days ago by
1sunmic2
• 0
1
vote
8
replies
787
views
Incorporating Salmon offset matrix into Wilcoxon DEG analysis
edgeR
17 days ago • updated 10 days ago
Andre
• 0
0
votes
2
replies
244
views
DESeq error: counts matrix should be numeric, currently it has mode: character
DESeq2
19 days ago • updated 18 days ago
Kucsera
• 0
0
votes
1
reply
169
views
combining raw counts and estimated counts
RNASeqData
GEO
TCGAbiolinks
updated 22 days ago by
O'Brien
• 0 • written 24 days ago by
Noor
• 0
0
votes
3
replies
1.2k
views
News:
EuroBioC2023 abstract submission closing soon!
presentation
EuroBioc2023
Conference
community
Bioconductor
written 21 months ago by
Maria Doyle
▴ 90
0
votes
5
replies
1.2k
views
News:
EuroBioC2024 abstract submission is open!
Conference
Bioconductor
EuroBioC2024
updated 21 days ago by
Alan Downey
• 0 • written 11 months ago by
Maria Doyle
▴ 90
0
votes
2
replies
442
views
Cross-validation with multiple control subgroups in limma
limma
multtest
28 days ago • updated 26 days ago
Ali Barry
▴ 40
1
vote
1
reply
357
views
Can DESeq2's design compensate for sequencing experimental design shortcomings?
DESeq2
updated 28 days ago by
Michael Love
43k • written 5 weeks ago by
txema.heredia
• 0
46,294 results • Page
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Comment: Problems with oarfish + tximport + swish
by
Michael Love
43k
Great thanks for posting the issue and sticking with it Annaleigh
Comment: Problems with oarfish + tximport + swish
by
brown.annaleigh
• 0
Awesome possum! Update works!! Thank you so much for the help and guidance!
Comment: Postdoctoral Researcher in Spatial Gene Regulatory Networks underlying Stomatal
by
iqratechnology4
• 0
Iqra Technology is IT Solutions and Services Company. We are salesforce and Microsoft partner company. https://iqratechnology.com/
Comment: Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
by
James W. MacDonald
67k
> BTW, shouldn't you be using keep.lib.sizes = F and re-do the calcNormFactors() after filtering of the dglst low-expression ENSGs, as Gord…
Comment: Problems with oarfish + tximport + swish
by
Michael Love
43k
Let me see if I can add some code to tximeta to cover this case. I've added an argument `skipRanges` in devel branch which should allow: …
Votes
Answer: Error in download.file for TCGAutils::symbolsToRanges
Answer: Error in download.file for TCGAutils::symbolsToRanges
Answer: Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
Answer: Differential expression of protein coding ENSGs (Ensembl IDs) | When to biomaRt?
Answer: Limma Interaction Effect with Covariates
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