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News:
Bioc2020 - Call for BOF/HAK/FAQ Sections
conference
bioc2020
talks
deadline
News
5.8 years ago • updated 5.6 years ago
shepherl
4.3k
1
vote
0
replies
1.4k
views
Pathview : why are some genes not colored?
pathview
rna-seq
5.6 years ago
okna0215
▴ 10
0
votes
6
replies
2.8k
views
Workaround to using normalized counts as input for edgeR
edger
updated 5.6 years ago by
Gordon Smyth
53k • written 5.6 years ago by
sharvari gujja
▴ 40
0
votes
7
replies
25k
views
DESeq2 batch correction best practice.
deseq2
limma
RUVSeq
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
94133
• 0
0
votes
1
reply
1.6k
views
ropls package: Recreating scores calculation from an OPLS-DA model
ropls
updated 5.6 years ago by
Kevin Blighe
★ 4.0k • written 5.6 years ago by
tor.demmers
• 0
0
votes
0
replies
1.7k
views
News:
Bioc2020 - Call for BOF/HAK/FAQ Sections Still Open
conference
bioc2020
talks
News
5.6 years ago
shepherl
4.3k
2
votes
6
replies
3.6k
views
How to run tximport ?
tximport
updated 5.6 years ago by
ATpoint
★ 5.0k • written 5.6 years ago by
sunnykevin97
• 0
0
votes
0
replies
760
views
How can I fix and run "sigCVError" function ?
sigFeature
svm-rfe
sigCVError
feature selection
5.6 years ago
rekren
▴ 20
3
votes
9
replies
7.6k
views
DESeq2: Clarification on independent filtering threshold values
deseq2
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
nattzy94
▴ 20
1
vote
12
replies
2.1k
views
Taking much longer time for same script after R upgrade to 4.0
R
5.6 years ago
hsiowa2
▴ 20
1
vote
2
replies
1.4k
views
question for "Chapter 24 Zeisel mouse brain (STRT-Seq) in single-cell"
STRT-Seq
single-cell
tutorial
updated 5.6 years ago by
Aaron Lun
★ 29k • written 5.6 years ago by
tiphaine.martin
• 0
1
vote
1
reply
632
views
Unable to run tximport using sample.info ?
tximport
deseq2
edgeR
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
sunnykevin97
• 0
0
votes
4
replies
2.0k
views
tximport importer isoform abundance
tximport
updated 2.7 years ago by
tangming2005
▴ 200 • written 5.6 years ago by
Ina Hoeschele
▴ 620
2
votes
2
replies
2.8k
views
Using GSVA All the pathway score are same
GSVA
scRNA-seq data
updated 5.6 years ago by
Robert Castelo
★ 3.4k • written 5.6 years ago by
initialqy
• 0
3
votes
5
replies
1.3k
views
How to loop over two files ...and calculate R^2 between SNPs
loops
R
updated 5.6 years ago by
James W. MacDonald
68k • written 5.6 years ago by
anamaria
▴ 10
3
votes
2
replies
1.1k
views
Differential methylation analysis and paired samples
edger
bisulfite sequencing
updated 5.6 years ago by
Gordon Smyth
53k • written 5.6 years ago by
tim.meese
• 0
2
votes
10
replies
5.5k
views
How to compare two or more GSVA outputs, when it is not possible to run GSVA at once
gsva
updated 5.6 years ago by
Robert Castelo
★ 3.4k • written 5.6 years ago by
hsiowa2
▴ 20
3
votes
2
replies
1.5k
views
How to get MS spectrum from features extracted with findChromPeaks ?
xcms
updated 5.6 years ago by
Johannes Rainer
★ 2.1k • written 5.6 years ago by
julien.kermorvant
▴ 30
0
votes
9
replies
2.6k
views
ReactomePA error graph
r
rnaseq
bulk
GO
ReactomePA
5.6 years ago
camillab.
▴ 10
0
votes
3
replies
1.3k
views
tximport cannot inport RSEM FPKM data
tximport
updated 4.2 years ago by
Michael Love
43k • written 5.6 years ago by
137737756
• 0
5
votes
10
replies
2.0k
views
Correctly set contrast with DESeq2 for not full rank design matrix
DESeq2
DGE
contrast
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
llooll1
• 0
3
votes
4
replies
1.1k
views
DGE analysis (statistics)
edger
limma
updated 5.6 years ago by
Gordon Smyth
53k • written 5.6 years ago by
sandravelasco911012
• 0
4
votes
4
replies
8.3k
views
plotPCA in DESeq2
software error
deseq2
5.6 years ago
yueli7
▴ 20
5
votes
11
replies
2.6k
views
Rsubread: change in behavior when accepting mixed paired end and single end files
software error
Rsubread
updated 5.6 years ago by
Gordon Smyth
53k • written 5.6 years ago by
barrel0luck
• 0
0
votes
2
replies
2.2k
views
PureCN/ASCAT comparison and purity/ploidy of multi-regional sequencing data
PureCN
ASCAT
purity
ploidy
multi-regional sequencing
5.6 years ago
twtoal
▴ 10
2
votes
2
replies
1.6k
views
docker image with VariantAnnotation package?
docker
updated 5.6 years ago by
Martin Morgan
25k • written 5.6 years ago by
Paul Shannon
▴ 470
0
votes
4
replies
2.4k
views
Wrong annotation (TSS/TTS) for many genes: keytype="GENEID" with mapIds(TxDb.Mmusculus.UCSC.mm10.knownGene)
AnnotationDbi
annotation
TxDb.Mmusculus.UCSC.mm10.knownGene
updated 5.6 years ago by
James W. MacDonald
68k • written 5.6 years ago by
94133
• 0
1
vote
0
replies
1.6k
views
Job:
Bioinformatics Data Analyst
R
RNA-seq
job
Job
5.6 years ago
António Miguel de Jesus Domingues
▴ 510
1
vote
1
reply
1.3k
views
DEXSeq-DTU normalisation suggestion - scaledTPM vs dtuScaledTPM
tximport
DEXSeq-DTU
DRIMSeq
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
aleksandar.baburski.g
• 0
0
votes
4
replies
2.6k
views
DiffBind or csaw between different transcription factors
DiffBind
csaw
updated 5.6 years ago by
Aaron Lun
★ 29k • written 5.6 years ago by
GFM
▴ 20
3
votes
1
reply
1.9k
views
Scaling option in fastMNN
fastmnn
scater
batchelor
updated 5.6 years ago by
Aaron Lun
★ 29k • written 5.6 years ago by
ATpoint
★ 5.0k
1
vote
1
reply
999
views
Problem in analyzing microarray with duplicate or triplicate data
microarray
limma
5.6 years ago
peirinl
• 0
1
vote
3
replies
963
views
Use Tx2Gene for Annotation
deseq2
annotation
r
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
pthom010
• 0
0
votes
9
replies
3.5k
views
R-devel - BiocGenerics - Warning: multiple methods tables found for which
BioGenerics
warning
5.6 years ago
ullrich
▴ 20
0
votes
1
reply
1.0k
views
PSEA deconvolution; some of the coefficient is NA?
PSEA
Deconvolution
5.6 years ago
kavator
▴ 30
0
votes
2
replies
1.4k
views
maSigPro Influential genes, step.methods and gene removal
maSigPro
TimeCourse
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
DcL-A
• 0
1
vote
2
replies
1.7k
views
DEXSeq dispersion estimation optimisation for large number of samples
DEXSeq
estimateDispersions
5.6 years ago
aleksandar.baburski.g
• 0
7
votes
10
replies
3.0k
views
is there a function in edger the same as vst or rlog in deseq2
edger
normalization
deseq2
updated 5.6 years ago by
Gordon Smyth
53k • written 5.6 years ago by
linouhao
▴ 20
2
votes
20
replies
4.4k
views
plotPCA in DESeq2
software error
deseq2
5.6 years ago
yueli7
▴ 20
0
votes
1
reply
553
views
Can't download DEDS Bioconducter V3.11, R V4.01
DEDS
updated 5.6 years ago by
James W. MacDonald
68k • written 5.6 years ago by
shui2
• 0
1
vote
5
replies
4.6k
views
Can't Download DESeq2 Package (Ubuntu 20.04; R 4.0.1; BioConductor 3.11)
deseq2
install
r4.0
bioconductor3.11
ubuntu20.04
5.6 years ago
ruwaa.ibrahem
• 0
1
vote
2
replies
2.4k
views
what does gseaparam argument in fgsea() means?
fgsea
updated 5.6 years ago by
alserg
▴ 280 • written 5.6 years ago by
kavator
▴ 30
0
votes
7
replies
3.0k
views
Filter Out Low Read Counts
deseq2
r
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
pthom010
• 0
0
votes
3
replies
664
views
What to do when two groups with untreated baseline show differentially expressed genes/taxa
deseq2
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
Puks
▴ 10
2
votes
2
replies
1.5k
views
biomart webservice error when using data from Phytozome
software error
go
5.6 years ago
e.lopez-cortegano
• 0
1
vote
2
replies
4.6k
views
How to do correlation analysis using DESeq2?
deseq2
updated 5.6 years ago by
Michael Love
43k • written 5.6 years ago by
zhaoy
• 0
1
vote
3
replies
1.3k
views
RSEM and Tximport
RSEM
tximport
deseq2
updated 5.6 years ago by
James W. MacDonald
68k • written 5.6 years ago by
pthom010
• 0
2
votes
10
replies
2.2k
views
How do you use the readBismark command in BiSeq properly?
BiSeq
DMR
updated 5.6 years ago by
James W. MacDonald
68k • written 5.6 years ago by
akhaira
• 0
0
votes
1
reply
949
views
Running DNAbarcodes with 12bp
dnabarcodes
updated 23 months ago by
nk130
• 0 • written 5.6 years ago by
roy.granit
• 0
1
vote
6
replies
14k
views
Change fontsize/remove name of annotation ComplexHeatmap package
r
rnaseq
complexHeatmap
annotation
updated 5.6 years ago by
Zuguang Gu
▴ 290 • written 5.6 years ago by
camillab.
▴ 10
46,725 results • Page
162 of 935
Recent ...
Replies
Answer: When to use edgeR or limma
by
Gordon Smyth
53k
The short answer is that we generally prefer edgeR for applications with lots of small counts and limma for complex designs with random eff…
Comment: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Gordon Smyth
53k
I updated the above answer today.
Answer: When to use edgeR or limma
by
James W. MacDonald
68k
I would use edgeR's quasi-likelihood model for any analysis with a smaller number of observations (like 3 vs 3 or similar), but if you have…
Answer: limpa-blank normalization and Spectronaut's PTM stoichiometry
by
Gordon Smyth
53k
The short answer is that limpa reads standard feature-level intensities from Spectronaut or other quantifications tools. limpa is designed …
Comment: Question about the filterByExpr function inputs in edgeR
by
mohammedtoufiq91
▴ 10
@gordonsmyth Noted, thank you very much.
Votes
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
A: deseq2 - paired samples in 2 sequencing types with very different library sizes
Comment: Question about the filterByExpr function inputs in edgeR
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