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DESEQ
•
reset
1
vote
2
replies
314
views
evaluate the variable importance on genes
DESeq2
deseq
10 weeks ago • updated 9 weeks ago
Meme
• 0
0
votes
2
replies
334
views
Running DESeq on normalized data
deseq
DESeq2
updated 4 months ago by
ATpoint
★ 4.1k • written 4 months ago by
Hugo
• 0
2
votes
2
replies
519
views
Predicting missing values splines DESeq2
deseq
DESeq2
5 months ago
georgii.vdovin
• 0
0
votes
1
reply
380
views
Controling for Covariates With a Continuous Predictor Variable
Deseq
DESeq2
updated 5 months ago by
Michael Love
41k • written 5 months ago by
georgii.vdovin
• 0
0
votes
1
reply
650
views
Can I transform already normalized rna-seq data with Z-score?
Z-score
RNASeqData
Deseq
DESeq2
updated 7 months ago by
James W. MacDonald
65k • written 7 months ago by
khaserdene99
• 0
0
votes
3
replies
887
views
Difference between DESeqDataSetFromMatrix() function and DESeq() function
DESeq
DESeq2
updated 9 months ago by
ATpoint
★ 4.1k • written 9 months ago by
Abir.khazaal
▴ 10
6
votes
11
replies
26k
views
With DESeq2 "Not full rank" Error with design ~ line + time + condition
deseq
deseq2
updated 9 months ago by
Erika
• 0 • written 9.3 years ago by
c.lieftink@nki.nl
▴ 40
0
votes
4
replies
655
views
lfc direction of effect flipped for ashr, apeGLM, but accurate to biological data for non-shrunken
DESeq2
apeglm
DESeq
ApeGLM
Ashr
updated 9 months ago by
Michael Love
41k • written 9 months ago by
Ndimensional
▴ 20
2
votes
5
replies
5.6k
views
ERROR configuration failed for package RCurl during installation of DESeq
ERROR: configuration failed for package ‘RCurl’
DESeq
written 4.1 years ago by
krunal.24.6
▴ 10
1
vote
2
replies
610
views
summarizeToGene in tximeta causing vastly different results
DESeq2
deseq
txime
tximeta
updated 12 months ago by
Michael Love
41k • written 12 months ago by
akrunic
• 0
0
votes
4
replies
1.2k
views
Volcano plot becomes flipped when I add a third condition
DESeq2
volcanoplot
deseq
HELP
contrast
updated 14 months ago by
Basti
▴ 780 • written 14 months ago by
Michael
• 0
0
votes
1
reply
565
views
What is the difference between these two arguments for log fold change shrinkage?
DESeq
updated 15 months ago by
Michael Love
41k • written 15 months ago by
m-bihie
▴ 10
0
votes
1
reply
724
views
Is it possible to convert transcript-level to gene-level dataset to use it in DESeq2 ?
DESeq2
Transcriptomics
tximport
deseq
txi
updated 15 months ago by
Dario Strbenac
★ 1.5k • written 15 months ago by
Hicham
▴ 10
0
votes
1
reply
2.8k
views
voom() vs. RPKM/FPKM or otherwise normalized counts, and GC correction, when fitting models to a small number of responses (per-feature counts)
Normalization
graph
limma
convert
edgeR
DESeq
Rsubread
Normalization
graph
limma
convert
updated 16 months ago by
Gordon Smyth
50k • written 11.9 years ago by
Kasper Daniel Hansen
★ 6.5k
0
votes
2
replies
1.2k
views
suggestions/comments on DESeq transcript wise analysis
convert
DESeq
convert
DESeq
written 12.2 years ago by
Akula, Nirmala NIH/NIMH [C]
▴ 190
1
vote
1
reply
519
views
How to design multifactor DESEQ2 formula?
DESEQ
DESEQ2
updated 20 months ago by
ATpoint
★ 4.1k • written 20 months ago by
barrypraveen
• 0
3
votes
2
replies
922
views
DESeq2 is renaming gene names and lack of downregulated genes
DESeq
DESeq2
21 months ago
manwar
• 0
1
vote
1
reply
779
views
Gene wise dispersion estimate Process - From the prior to the posterior
GLM
prior
DESeq
LFC
updated 22 months ago by
Michael Love
41k • written 22 months ago by
stew
• 0
1
vote
5
replies
1.2k
views
Error in DEseq
DEseq
RNASeqData
updated 23 months ago by
ATpoint
★ 4.1k • written 23 months ago by
Sanatan
• 0
2
votes
1
reply
1.5k
views
Difference between raw counts and featureCounts for DESeq2
deseq
featurecounts
counts
DESeq2
updated 23 months ago by
swbarnes2
★ 1.3k • written 23 months ago by
Hicham
▴ 10
1
vote
5
replies
1.4k
views
Potential outlier/high variance
deseq
DESeq2
updated 2.0 years ago by
Michael Love
41k • written 2.1 years ago by
Amali Thrimawithana
• 0
1
vote
2
replies
913
views
Deseq2 with imbalanced design
Deseq
2.1 years ago
Alex
• 0
1
vote
4
replies
1.6k
views
Test if a gene is NOT differentially expressed in DESeq2?
DESeq2
deseq
updated 2.3 years ago by
Michael Love
41k • written 2.3 years ago by
penny.lane
• 0
0
votes
2
replies
1.1k
views
DESeqDataSet design issue
DESeq
DESeq2
2.4 years ago
matthew.valentine
• 0
19
votes
15
replies
58k
views
DESeq2 output explanation
deseq
deseq2
updated 2.5 years ago by
Michael Love
41k • written 9.5 years ago by
francesca.defilippis
▴ 100
9
votes
6
replies
10k
views
Error bars DESeq or DESeq2 fold change
rnaseq
deseq2
deseq
standard error
confidence interval
updated 2.5 years ago by
Michael Love
41k • written 8.1 years ago by
lisa.crossman
▴ 10
1
vote
1
reply
1.5k
views
DESeq2 rows did not converge in beta, how to solve
DESeq
DESeq2
updated 2.5 years ago by
Michael Love
41k • written 2.5 years ago by
n.vandis
• 0
4
votes
6
replies
1.7k
views
Interpretation of DESeq2 results with 3 factorial design with interaction terms.
r
DESeq
DESeq2
2.6 years ago
s-rhussien
• 0
2
votes
2
replies
2.3k
views
Correct way to make multiple comparisons on DESeq2?
DESeq
DESeq2
R
2.7 years ago • updated 2.6 years ago
jbnrodriguez
▴ 10
1
vote
2
replies
982
views
DESeq2 log2Foldchanges opposite sign for almost the same groups of comparison
RNASeq
DESeq
2.7 years ago
krawczyk.margarita
• 0
0
votes
2
replies
1.5k
views
same padj for all the genes after DEseq analysis
etLRT
WALD
edgeR
DESEq
updated 2.7 years ago by
Michael Love
41k • written 2.7 years ago by
aiswaryabioinfo
• 0
0
votes
1
reply
1.1k
views
Filtering DESeq2 results for | LFC | > 1 genes
RNAseq
LFC
DEseq
2.9 years ago
ayy2110
• 0
0
votes
7
replies
13k
views
Applying DESeq on RSEM output
edgeR
DESeq
edgeR
DESeq
updated 3.1 years ago by
mehdimerbah
• 0 • written 11.1 years ago by
dvir.tau@gmail.com
▴ 30
1
vote
2
replies
939
views
metage problem with DESeq
DESeq
metagene2
metagene
updated 3.1 years ago by
ericfournier2
▴ 10 • written 3.2 years ago by
GM
▴ 10
10
votes
14
replies
13k
views
how DESeq calculates p-value ?
deseq
differential gene expression
statistics
p-value
updated 3.2 years ago by
Kai_Qi
▴ 20 • written 8.2 years ago by
Giard
▴ 30
0
votes
3
replies
2.2k
views
Understanding MA plot distortion
DESeq2
MAplot
deseq
updated 3.2 years ago by
Michael Love
41k • written 3.2 years ago by
sp
• 0
0
votes
5
replies
1.7k
views
ZINB-WaVE-DESeq2 with Bulk Data: Choice of Epsilon value, K and gene number
DESeq2
ZINB
zinbwave
deseq
updated 3.2 years ago by
Michael Love
41k • written 3.2 years ago by
rowcyclecamp
• 0
2
votes
12
replies
4.5k
views
Deseq2: How to adjust for baseline read counts when testing for groups differences at post-treatment
deseq2
deseq
updated 3.5 years ago by
Michael Love
41k • written 9.0 years ago by
Nick
• 0
1
vote
2
replies
979
views
DESeq Error (nbinomTest ())
Deseq
nbinomTest
3.9 years ago
canfield
▴ 10
0
votes
7
replies
1.8k
views
DESeq error: Error in validityMethod(object) : nrow(design) == ncol(object) is not TRUE
deseq2
DESeq
nrow == ncol is not TRUE
updated 3.9 years ago by
Kevin Blighe
★ 3.9k • written 3.9 years ago by
maniermk
• 0
2
votes
2
replies
862
views
Mocking data for understanding interactions in DESeq2
deseq2
deseq
updated 4.1 years ago by
Simon Anders
★ 3.7k • written 4.1 years ago by
blawney
• 0
3
votes
5
replies
2.1k
views
How to analyze pre-processed RNA-Seq data from GEO based on the deseq R package for filtering and DE analysis
deseq
rna-seq
filtering
DE
deseq2
updated 4.1 years ago by
Michael Love
41k • written 4.1 years ago by
svlachavas
▴ 830
1
vote
9
replies
1.3k
views
Wrong results of DESeq compared to DESeq2 for some test cases?
deseq2
DESeq
updated 4.1 years ago by
Simon Anders
★ 3.7k • written 4.1 years ago by
megapode32559
• 0
0
votes
7
replies
1.1k
views
how to analyze and find differentially expressed genes based on miRNA gene raw read counts?
deseq2
deseq
4.3 years ago
MANYA_BIOINFO
• 0
0
votes
1
reply
1.2k
views
DESeq2 counts function not working
deseq2
Deseq
RNAseq
updated 4.4 years ago by
Michael Love
41k • written 4.4 years ago by
mrfossl97
▴ 10
2
votes
3
replies
1.4k
views
DESeq2 2x2 factorial design: find genes unique to factor A?
deseq2
deseq
differential expression
contrasts
updated 4.5 years ago by
Michael Love
41k • written 4.5 years ago by
Keith Hughitt
▴ 180
4
votes
4
replies
4.5k
views
TMM Normalization: DESeqDataSet: some values in assay are negative
deseq2
deseq
normalization
updated 4.7 years ago by
wunderl
▴ 40 • written 4.8 years ago by
neekonsu
• 0
1
vote
2
replies
889
views
Filtering extraneous samples affects DESeq results. What is going on?
deseq
deseq2
updated 4.8 years ago by
Michael Love
41k • written 4.8 years ago by
sxv
• 0
0
votes
0
replies
940
views
Including sva results in SeqGSEA with data with batch effect
limma
deseq2
seqgsea
sva
deseq
4.8 years ago
kentfung
▴ 20
0
votes
9
replies
2.0k
views
NOOB question: How to use contrasts for subgroup analysis in DESeq2?
deseq
deseq2
contrast
subgroup
5.2 years ago
ariel
▴ 20
50 results • Page
1 of 1
Recent ...
Replies
Comment: Although I successfully installed the package "simpleaffy'' , I cannot library i
by
fatinataly
• 0
I tried also with oligo does not work, any other suggestion?
Comment: More regions in union when increasing DBA$config$mergeOverlap
by
Ian D.
▴ 70
Hello. Thanks for the reply. Sorry about this. My testing is telling me that 1 is the default (a gap), not -1 (1bp overlap). Negative …
Comment: Differences in differential analysis methods for RNA-Seq
by
ATpoint
★ 4.1k
> I am just re-processing due to being uncertain about how they used the edgeR analysis to reach gene level edgeR devs published something…
Comment: Differences in differential analysis methods for RNA-Seq
by
james.zhang20
• 0
Thanks for the explanations, it has helped clarify some things. Unfortunately, I am working from an early access public dataset (https://do…
Answer: Deseq2 and Normalization of RNA sequencing data
by
ATpoint
★ 4.1k
There is no expectation of normality that is realistic in terms of expression level. Levels are widely different between genes, and counts …
Votes
A: Error in DESeqDataSet : some values in assay are not integers
Bioconductor 3.19 is Released!
Bioconductor 3.19 is Released!
Answer: DEseq2 coefficient
SPIA plotP giving error
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