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DESeq2
•
reset
1
vote
8
replies
139
views
Slightly different count plots from R and spreadsheet
DESeq2
ggplot2
1 day ago • updated 21 hours ago
boczniak767
▴ 720
1
vote
3
replies
137
views
LRT, more than two conditions and pairwise comparisons
DESeq2
updated 2 days ago by
James W. MacDonald
66k • written 4 days ago by
boczniak767
▴ 720
2
votes
4
replies
217
views
DESeq2- Mysterious interaction suppresses DEGs
DESeq2
PCAplot
RNA-Seq
DesignModel
updated 3 days ago by
Michael Love
42k • written 6 days ago by
shaunpeterson
• 0
0
votes
6
replies
595
views
Numerical differences in DESeq2 output depending on different design matrix construction
DESeq2
6 days ago • updated 3 days ago
Ken C
• 0
1
vote
2
replies
271
views
How can I create a eigencorplot of pca created from deseq2 object with PCAtools
DESeq2
eigencorplot
PCAtools
9 days ago • updated 7 days ago
BioinfGuru
▴ 30
4
votes
9
replies
313
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 7 days ago by
ATpoint
★ 4.2k • written 8 days ago by
pangtaihin
• 0
0
votes
3
replies
154
views
Assymetric MA plot with no overall trend
DESeq2
DifferentialExpression
updated 8 days ago by
Michael Love
42k • written 9 days ago by
Kasper
• 0
0
votes
8
replies
557
views
Can I trust the differential expression analysis when the two groups overlap on the PCA?
DESeq2
pcaExplorer
updated 8 days ago by
BioinfGuru
▴ 30 • written 10 days ago by
Eric Katagirya
• 0
0
votes
1
reply
117
views
normalization across different species for DESeq2 analysis
DESeq2
normalization
Normalization
acrossspecies
updated 9 days ago by
Michael Love
42k • written 10 days ago by
piemmea
• 0
2
votes
7
replies
443
views
deseq2 results
DESeq2
DifferentialExpression
rnaseqGene
DifferentialRegulation
15 days ago • updated 12 days ago
sajadahmad41454
• 0
0
votes
2
replies
1.5k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 13 days ago by
maria.soler
• 0 • written 16 months ago by
kalavattam
▴ 10
0
votes
1
reply
192
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 13 days ago by
ATpoint
★ 4.2k • written 13 days ago by
sajadahmad41454
• 0
0
votes
1
reply
120
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 14 days ago by
ATpoint
★ 4.2k • written 14 days ago by
sajadahmad41454
• 0
1
vote
3
replies
320
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 15 days ago by
ATpoint
★ 4.2k • written 21 days ago by
angelathuynh5
• 0
1
vote
1
reply
199
views
Handling multiple differential expression comparisons
DESeq2
updated 18 days ago by
Michael Love
42k • written 20 days ago by
Zainab
▴ 10
2
votes
3
replies
267
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 18 days ago by
ATpoint
★ 4.2k • written 20 days ago by
Reza
• 0
0
votes
6
replies
312
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 19 days ago by
swbarnes2
★ 1.4k • written 20 days ago by
arfranco
▴ 130
0
votes
2
replies
176
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
23 days ago • updated 20 days ago
Aurora
• 0
0
votes
2
replies
219
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
21 days ago • updated 20 days ago
kcarey
• 0
0
votes
2
replies
182
views
DESeq2 v1.35.0
DESeq2
updated 21 days ago by
Michael Love
42k • written 21 days ago by
Espresso
• 0
0
votes
3
replies
238
views
Differences in differential analysis methods for RNA-Seq
DESeq2
StatisticalMethod
Proteomics
DifferentialExpression
RNASeq
updated 21 days ago by
ATpoint
★ 4.2k • written 21 days ago by
james.zhang20
• 0
0
votes
3
replies
309
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 21 days ago by
Michael Love
42k • written 22 days ago by
HAK
• 0
1
vote
4
replies
537
views
DEseq2 coefficient
DESeq2
coefficient
12 weeks ago • updated 22 days ago
JKim
• 0
6
votes
19
replies
2.6k
views
DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
deseq2
updated 23 days ago by
Sey R.
• 0 • written 4.6 years ago by
hsbio
▴ 10
1
vote
1
reply
162
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
updated 23 days ago by
Michael Love
42k • written 23 days ago by
marcusn
• 0
0
votes
1
reply
143
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 23 days ago by
Michael Love
42k • written 23 days ago by
Agung
• 0
0
votes
1
reply
143
views
putting my own expression data in deseq2
DESeq2
updated 23 days ago by
ATpoint
★ 4.2k • written 23 days ago by
Dev
• 0
3
votes
3
replies
279
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 24 days ago by
ATpoint
★ 4.2k • written 24 days ago by
bioinf
▴ 10
0
votes
2
replies
232
views
PCA plot suggestions
DESeq2
PCAtools
updated 27 days ago by
Michael Love
42k • written 28 days ago by
Aaliya
• 0
1
vote
2
replies
767
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
5 weeks ago • updated 27 days ago
Patadù
▴ 10
1
vote
3
replies
261
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 27 days ago by
Michael Love
42k • written 27 days ago by
JalapenoCornbread
• 0
1
vote
4
replies
365
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
29 days ago • updated 27 days ago
winwater0928
• 0
2
votes
9
replies
444
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 27 days ago by
James W. MacDonald
66k • written 28 days ago by
Alexandre
• 0
0
votes
8
replies
407
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 28 days ago by
swbarnes2
★ 1.4k • written 4 weeks ago by
Aaliya
• 0
0
votes
1
reply
170
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 28 days ago by
Michael Love
42k • written 29 days ago by
kcarey
• 0
1
vote
3
replies
243
views
Different Shrunk Log2FC according with reference
DESeq2
updated 29 days ago by
Michael Love
42k • written 29 days ago by
andrebolerbarros
▴ 20
3
votes
1
reply
335
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 29 days ago by
Michael Love
42k • written 4 weeks ago by
i.sudbery
▴ 40
3
votes
3
replies
262
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 4 weeks ago by
WardDeb
• 0
0
votes
4
replies
273
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
5 weeks ago • updated 4 weeks ago
lonn
• 0
1
vote
2
replies
273
views
How to retrieve detailed regression results from deseq2
DESeq2
6 weeks ago • updated 4 weeks ago
Weiqian
▴ 10
0
votes
7
replies
461
views
DESeq filtering specific to contrasts
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 3 months ago by
Emma
• 0
2
votes
5
replies
803
views
DESeq2 setting significant p-value
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 5.0 years ago by
Merlin
▴ 10
0
votes
4
replies
296
views
DEG with low counts
DESeq2
updated 4 weeks ago by
Michael Love
42k • written 5 weeks ago by
Rajat
• 0
0
votes
1
reply
160
views
rlog transformation
DESeq2
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
ishij9717
• 0
0
votes
1
reply
133
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
09191919
• 0
0
votes
1
reply
151
views
covariate with negative and positive values
DESeq2
updated 5 weeks ago by
Michael Love
42k • written 5 weeks ago by
Brynn
• 0
9
votes
12
replies
7.9k
views
Multi factor design edgeR/Deseq2
deseq2
edgeR
rna-seq
updated 5 weeks ago by
Elizabeth
• 0 • written 8.9 years ago by
gthm
▴ 30
0
votes
1
reply
171
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
updated 5 weeks ago by
ATpoint
★ 4.2k • written 5 weeks ago by
quanah.hudson
• 0
2
votes
5
replies
1.2k
views
DESeq2 remove batch effect
BatchEffect
RNASeqData
WGCNA
DESeq2
updated 5 weeks ago by
Ashley
• 0 • written 11 months ago by
zxc741xb
• 0
0
votes
4
replies
638
views
How to correct for known and surrogate variables in DESeq2?
DESeq2
BatchEffect
sva
limma
updated 5 weeks ago by
Michael Love
42k • written 7 months ago by
Crystal571
• 0
50 results • Page
1 of 1
Recent ...
Replies
Comment: Using the plotting functions of clusterProfiler / enrichplot with enrichment res
by
jovel_juan
▴ 30
enrichPlot requirements are rather low. All what it needs is to provide few columns named specifically. I student of mine wrote the first v…
Comment: Using featureCounts with SAF annotation file gives "Error: only GTF files contai
by
ATpoint
★ 4.2k
SAF files must not have a header.
Comment: Running ENmix with mouse methylation manifest: readidat error - Error in readman
by
xuz
• 0
Could you try the latest version (1.40.1) to see how it works, I am not able to replicate the error message on my computer here.
Comment: Not able to install any Bioconductor 3.18 package [unable to access index]
by
merv
▴ 120
Thanks! I can confirm it is working now - had been broken since sometime yesterday.
Comment: Errors in chromosome annotation-biomaRt
by
landenshanie
▴ 40
Thank you, I ran your exact code and it correct for Xist, however Ddx3y is still wrongly annotated to chr13 and, like your results, Dusp1 i…
Votes
Answer: Not able to install any Bioconductor 3.18 package [unable to access index]
Seurat/DESeq2 - single cell RNA-seq differential expression
Seurat to SingleCellExperiment Converting error in MAC
Error when converting Seurat to SingleCellExperiment
Comment: unable to access bioconductor packages - qvalue installation failed...
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